Restriction map for
pFN21AE2745
(Circular) (Six-base) MAPSORT of: pFN21AE2745.seq Check: 1723 from: 1 to: 5866
pFN21AE2745 5866 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3209 1937
Size: 1272 4594
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2843 2901 3929 5313 5581 1632
Size: 1211 58 1028 1384 268 1917
Fragments arranged by size:
1917 1384 1211 1028 268 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 2129 3083 1166
Size: 963 954 3949
Fragments arranged by size:
3949 963 954
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 2218 3609 5181 2218
Size: 1391 1572 2903
Fragments arranged by size:
2903 1572 1391
ApaI G_GGCC'C
Cuts at: 2491 2685 3105 2491
Size: 194 420 5252
Fragments arranged by size:
5252 420 194
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 2028 3175 3401 3553 5662 1493
Size: 535 1147 226 152 2109 1697
Fragments arranged by size:
2109 1697 1147 535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 2637 3126 3191 1870
Size: 73 694 489 65 4545
Fragments arranged by size:
4545 694 489 73 65
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3187 4642 618
Size: 325 217 627 1400 1455 1842
Fragments arranged by size:
1842 1455 1400 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2148 2280 2453 2491 2501 2685 2879 2976
Size: 757 132 173 38 10 184 194 97
Cuts at: 2976 2996 3105 3185 1391
Size: 20 109 80 4072
Fragments arranged by size:
4072 757 194 184 173 132 109 97
80 38 20 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2622 928
Size: 971 723 4172
Fragments arranged by size:
4172 971 723
BbvCI CC'TCA_GC
Cuts at: 2354 2354
Size: 5866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 2560 1548
Size: 1012 4854
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4477 136
Size: 107 122 71 631 605 2805 1525
Fragments arranged by size:
2805 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 2495 3283 2495
Size: 788 5078
Bme1580I G_kGCm'C
Cuts at: 1790 2491 2685 3105 3927 5083 1790
Size: 701 194 420 822 1156 2573
Fragments arranged by size:
2573 1156 822 701 420 194
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2679 3597 4555
Size: 416 53 83 399 24 1262 918 958
Cuts at: 4555 442
Size: 1753
Fragments arranged by size:
1753 1262 958 918 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4526 1239
Size: 3287 2579
Bpu10I CC'TnA_GC
Cuts at: 2274 2354 2274
Size: 80 5786
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2657 3991 4856 5154 2657
Size: 1334 865 298 3369
Fragments arranged by size:
3369 1334 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2639 3068 4529 882
Size: 617 1140 429 1461 2219
Fragments arranged by size:
2219 1461 1140 617 429
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 5860 5860
Size: 5866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2052 2748 2976 4299 4697 4971 5118 5529 2052
Size: 696 228 1323 398 274 147 411 2389
Fragments arranged by size:
2389 1323 696 411 398 274 228 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5641
Fragments arranged by size:
5641 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5866
BseYI C'CCAG_C
Cuts at: 2283 2446 2490 2500 2629 2908 5069 5565 2283
Size: 163 44 10 129 279 2161 496 2584
Fragments arranged by size:
2584 2161 496 279 163 129 44 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2344 2372 2652 1163
Size: 573 608 28 280 4377
Fragments arranged by size:
4377 608 573 280 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3026 3316 4077 5105 1540
Size: 275 186 1025 290 761 1028 2301
Fragments arranged by size:
2301 1028 1025 761 290 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2280 2976 3927 4012 5083 2148
Size: 132 696 951 85 1071 2931
Fragments arranged by size:
2931 1071 951 696 132 85
BsmI GAATG_Cn'
Cuts at: 2412 3377 3470 2412
Size: 965 93 4808
Fragments arranged by size:
4808 965 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2280 2453 2491 2501 2685 2879
Size: 399 358 132 173 38 10 184 194
Cuts at: 2879 2976 2996 3105 3185 3927 4012 5083 1391
Size: 97 20 109 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 194 184 173
132 109 97 85 80 38 20 10
BspEI T'CCGG_A
Cuts at: 2748 2976 5529 2748
Size: 228 2553 3085
Fragments arranged by size:
3085 2553 228
BspHI T'CATG_A
Cuts at: 2178 3755 5485 2178
Size: 1577 1730 2559
Fragments arranged by size:
2559 1730 1577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2277 2382 3221 844
Size: 1433 105 839 3489
Fragments arranged by size:
3489 1433 839 105
BsrBI CCG'CTC
Cuts at: 1394 1823 2105 3753 1394
Size: 429 282 1648 3507
Fragments arranged by size:
3507 1648 429 282
BsrDI GCAATG_nn'
Cuts at: 65 1248 4361 4535 65
Size: 1183 3113 174 1396
Fragments arranged by size:
3113 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2508 4510 4697 1700
Size: 110 698 2002 187 2869
Fragments arranged by size:
2869 2002 698 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 3926 4938 3926
Size: 1012 4854
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2521 794
Size: 1727 4139
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 3196 3563 3948 3965 5417 5503 5515 5861
Size: 1224 367 385 17 1452 86 12 346
Cuts at: 5861 1972
Size: 1977
Fragments arranged by size:
1977 1452 1224 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2583 2778 2583
Size: 195 5671
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 513
Size: 552 939 120 4255
Fragments arranged by size:
4255 939 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2725 487
Size: 683 56 1499 3628
Fragments arranged by size:
3628 1499 683 56
BtsI GCAGTG_nn'
Cuts at: 2384 2903 3070 3382 4176 4196 2384
Size: 519 167 312 794 20 4054
Fragments arranged by size:
4054 794 519 312 167 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DraIII CAC_nnn'GTG
Cuts at: 2119 3011 2119
Size: 892 4974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 3023 3313
Size: 54 1425 50 275 18 366 827 290
Cuts at: 3313 4202 5831 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 827 366 290 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3023 3313 1537
Size: 275 1211 290 4090
Fragments arranged by size:
4090 1211 290 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2762 2877 3796 1353
Size: 1409 115 919 3423
Fragments arranged by size:
3423 1409 919 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2278 2974 2146
Size: 132 696 5038
Fragments arranged by size:
5038 696 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2843 2901 3929 4526 5313 5581 1239
Size: 393 1211 58 1028 597 787 268 1524
Fragments arranged by size:
1524 1211 1028 787 597 393 268 58
EcoO109I rG'GnC_Cy
Cuts at: 2371 2487 2570 2681 3101 3102 2371
Size: 116 83 111 420 1 5135
Fragments arranged by size:
5135 420 116 111 83 1
EcoRI G'AATT_C
Cuts at: 2028 3175 3553 2028
Size: 1147 378 4341
Fragments arranged by size:
4341 1147 378
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 5013 1678
Size: 3335 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4507 4539 4581 4613 1373
Size: 32 135 32 2935 32 42 32 2626
Fragments arranged by size:
2935 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3210 3456 669
Size: 988 281 1272 246 3079
Fragments arranged by size:
3079 1272 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2778 3168 4980 5164 1305
Size: 1473 390 1812 184 2007
Fragments arranged by size:
2007 1812 1473 390 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 10
Size: 54 1425 343 366 3678
Fragments arranged by size:
3678 1425 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2327 2925 3083 3824 4183 4342
Size: 648 963 198 598 158 741 359 159
Cuts at: 4342 518
Size: 2042
Fragments arranged by size:
2042 963 741 648 598 359 198 159
158
MspA1I CmG'CkG
Cuts at: 1606 2090 2358 2494 3282 3959 5107 5352 1606
Size: 484 268 136 788 677 1148 245 2120
Fragments arranged by size:
2120 1148 788 677 484 268 245 136
NaeI GCC'GGC
Cuts at: 2510 2510
Size: 5866
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NgoMIV G'CCGG_C
Cuts at: 2508 2508
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 2524 3092 3224 4769 1132
Size: 97 1295 568 132 1545 2229
Fragments arranged by size:
2229 1545 1295 568 132 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5866
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5866
PfoI T'CCnGG_A
Cuts at: 2367 2367
Size: 5866
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 2570 2371
Size: 199 5667
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PspOMI G'GGCC_C
Cuts at: 2487 2681 3101 2487
Size: 194 420 5252
Fragments arranged by size:
5252 420 194
PstI C_TGCA'G
Cuts at: 830 2223 3218 830
Size: 1393 995 3478
Fragments arranged by size:
3478 1393 995
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
PvuII CAG'CTG
Cuts at: 2090 2358 2494 2090
Size: 268 136 5462
Fragments arranged by size:
5462 268 136
SacI G_AGCT'C
Cuts at: 2148 2280 2976 2148
Size: 132 696 5038
Fragments arranged by size:
5038 696 132
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SanDI GG'GwC_CC
Cuts at: 2570 2570
Size: 5866
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 2219 3214 4349 5030 5221 826
Size: 219 1174 995 1135 681 191 1471
Fragments arranged by size:
1471 1174 1135 995 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 2508 1700
Size: 808 5058
SmaI CCC'GGG
Cuts at: 1872 2639 3128 3193 1872
Size: 767 489 65 4545
Fragments arranged by size:
4545 767 489 65
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2672 3970 4871 5133 820
Size: 197 926 729 1298 901 262 1553
Fragments arranged by size:
1553 1298 926 901 729 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3092 3224 1132
Size: 1960 132 3774
Fragments arranged by size:
3774 1960 132
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 2004 2397 3305 513
Size: 552 939 393 908 3074
Fragments arranged by size:
3074 939 908 552 393
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2555 3893 3910 4063 4248 4693 325
Size: 1255 975 1338 17 153 185 445 1498
Fragments arranged by size:
1498 1338 1255 975 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4112 5824 96
Size: 274 80 33 51 1443 2135 1712 138
Fragments arranged by size:
2135 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2105 2137 5826 5858 2105
Size: 32 3689 32 2113
Fragments arranged by size:
3689 2113 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 3113 2443
Size: 670 5196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 2637 3126 3191 1870
Size: 767 489 65 4545
Fragments arranged by size:
4545 767 489 65
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI