Restriction map for
pFN21AE2741
(Circular) (Six-base) MAPSORT of: pFN21AE2741.seq Check: 2164 from: 1 to: 5782
pFN21AE2741 5782 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2589 3592 278
Size: 53 83 186 936 1053 1003 2468
Fragments arranged by size:
2468 1053 1003 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2997 3845 5229 5497 1632
Size: 1365 848 1384 268 1917
Fragments arranged by size:
1917 1384 1365 848 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 2845 3525 5097 2845
Size: 680 1572 3530
Fragments arranged by size:
3530 1572 680
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2180 2363 2904 3091 3317 3469 5578 1493
Size: 687 183 541 187 226 152 2109 1697
Fragments arranged by size:
2109 1697 687 541 226 187 183 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 3107 1870
Size: 73 87 1077 4545
Fragments arranged by size:
4545 1077 87 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2915 3103 4558 618
Size: 325 217 627 1128 188 1455 1842
Fragments arranged by size:
1842 1455 1128 627 325 217 188
BanII G_rGCy'C
Cuts at: 1391 2828 2961 3101 1391
Size: 1437 133 140 4072
Fragments arranged by size:
4072 1437 140 133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 2985 928
Size: 971 347 739 3725
Fragments arranged by size:
3725 971 739 347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3973 4007 1903
Size: 34 50 34 1952 34 3678
Fragments arranged by size:
3678 1952 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2687 4393 136
Size: 107 122 71 631 605 1015 1706 1525
Fragments arranged by size:
1706 1525 1015 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2821 3843 4999 1790
Size: 1031 1022 1156 2573
Fragments arranged by size:
2573 1156 1031 1022
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2039 2288 2427 2455 2558 2786 2793
Size: 794 6 249 139 28 103 228 7
Cuts at: 2793 4442 1239
Size: 1649 2579
Fragments arranged by size:
2579 1649 794 249 228 139 103 28
7 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2586 3589 3971 4606
Size: 53 83 186 936 1053 1003 382 635
Cuts at: 4606 275
Size: 1451
Fragments arranged by size:
1451 1053 1003 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2908 4215 4613 4887 5034 5445 2908
Size: 1307 398 274 147 411 3245
Fragments arranged by size:
3245 1307 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5557
Fragments arranged by size:
5557 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2327 1186
Size: 1141 4641
BseYI C'CCAG_C
Cuts at: 2037 2875 4985 5481 2037
Size: 838 2110 496 2338
Fragments arranged by size:
2338 2110 838 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 3843 3928 4999 2256
Size: 1587 85 1071 3039
Fragments arranged by size:
3039 1587 1071 85
BsiWI C'GTAC_G
Cuts at: 2244 2244
Size: 5782
BsmI GAATG_Cn'
Cuts at: 2227 3293 3386 2227
Size: 1066 93 4623
Fragments arranged by size:
4623 1066 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2821 2828 2961 3101 3843 3928
Size: 399 466 565 7 133 140 742 85
Cuts at: 3928 4999 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 565 466 399 140 133
85 7
BspEI T'CCGG_A
Cuts at: 2908 5445 2908
Size: 2537 3245
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2601 3137 844
Size: 1757 536 3489
Fragments arranged by size:
3489 1757 536
BsrBI CCG'CTC
Cuts at: 1394 1823 2572 3669 1394
Size: 429 749 1097 3507
Fragments arranged by size:
3507 1097 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2067 2872 4277 4451 65
Size: 1183 819 805 1405 174 1396
Fragments arranged by size:
1405 1396 1183 819 805 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2845 794
Size: 2051 3731
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2707 2011
Size: 696 5086
BstYI r'GATC_y
Cuts at: 1972 2322 2402 3112 3479 3864 3881 5333 5419
Size: 350 80 710 367 385 17 1452 86
Cuts at: 5419 5431 5777 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 710 385 367 350 346 86
80 17 12
Bsu36I CC'TnA_GG
Cuts at: 2580 2580
Size: 5782
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 2331 2558 2691 3298 4092 4112 2331
Size: 227 133 607 794 20 4001
Fragments arranged by size:
4001 794 607 227 133 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2122 2157 529
Size: 35 1558 35 4154
Fragments arranged by size:
4154 1558 35 35
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2130 2679
Size: 54 1425 50 275 18 250 50 549
Cuts at: 2679 2781 2892 3229 4118 5747 8
Size: 102 111 337 889 1629 43
Fragments arranged by size:
1629 1425 889 549 337 275 250 111
102 54 50 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2162 3018 3712 1353
Size: 809 856 694 3423
Fragments arranged by size:
3423 856 809 694
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2039 2288 2427 2455 2558 2786
Size: 393 401 6 249 139 28 103 228
Cuts at: 2786 2793 2997 3845 4442 5229 5497 1239
Size: 7 204 848 597 787 268 1524
Fragments arranged by size:
1524 848 787 597 401 393 268 249
228 204 139 103 28 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2206 2206
Size: 5782
EcoO109I rG'GnC_Cy
Cuts at: 3007 3007
Size: 5782
EcoRI G'AATT_C
Cuts at: 2180 2363 3091 3469 2180
Size: 183 728 378 4493
Fragments arranged by size:
4493 728 378 183
EcoRV GAT'ATC
Cuts at: 2673 2673
Size: 5782
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2038 4929 1678
Size: 360 2891 2531
Fragments arranged by size:
2891 2531 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373
Size: 32 135 32 2851 32 42 32 2626
Fragments arranged by size:
2851 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2590 3126 3372 669
Size: 988 281 652 536 246 3079
Fragments arranged by size:
3079 988 652 536 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2380 2688 4896 5080 1305
Size: 1075 308 2208 184 2007
Fragments arranged by size:
2208 2007 1075 308 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 2894 10
Size: 54 1425 343 300 762 2898
Fragments arranged by size:
2898 1425 762 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3038 3740 4099 4258 518
Size: 648 1872 702 359 159 2042
Fragments arranged by size:
2042 1872 702 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2842 3198 3875 5023 5268 1606
Size: 405 831 356 677 1148 245 2120
Fragments arranged by size:
2120 1148 831 677 405 356 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2938 3140 4685 1132
Size: 97 1709 202 1545 2229
Fragments arranged by size:
2229 1709 1545 202 97
PasI CC'CwG_GG
Cuts at: 1348 2821 1348
Size: 1473 4309
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3007 3007
Size: 5782
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2301 3134 830
Size: 1471 833 3478
Fragments arranged by size:
3478 1471 833
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2842 2842
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2297 3130 4265 4946 5137 826
Size: 219 1252 833 1135 681 191 1471
Fragments arranged by size:
1471 1252 1135 833 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 2032 3109 1872
Size: 160 1077 4545
Fragments arranged by size:
4545 1077 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2133 2202 2700 3221 513
Size: 552 1068 69 498 521 3074
Fragments arranged by size:
3074 1068 552 521 498 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 2942 3809 3826 3979 4164 4609
Size: 1255 449 913 867 17 153 185 445
Cuts at: 4609 325
Size: 1498
Fragments arranged by size:
1498 1255 913 867 449 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2286 4028 5740
Size: 274 80 33 51 1443 309 1742 1712
Cuts at: 5740 96
Size: 138
Fragments arranged by size:
1742 1712 1443 309 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2731 2763 5742 5774 2731
Size: 32 2979 32 2739
Fragments arranged by size:
2979 2739 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2926 2926
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 2030 3107 1870
Size: 160 1077 4545
Fragments arranged by size:
4545 1077 160
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2587 3590 276
Size: 53 83 186 936 1053 1003 2468
Fragments arranged by size:
2468 1053 1003 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI