Restriction map for
pFN21AE2734
(Circular) (Six-base) MAPSORT of: pFN21AE2734.seq Check: 5645 from: 1 to: 5644
pFN21AE2734 5644 bp
With 182 enzymes: SgfI *
September 25, 2018 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2146 2746 2146
Size: 600 5044
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2916 2965 2916
Size: 49 5595
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 3707 5091 5359 1632
Size: 551 1524 1384 268 1917
Fragments arranged by size:
1917 1524 1384 551 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2475 4543 5520 1203
Size: 1272 2068 977 1327
Fragments arranged by size:
2068 1327 1272 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 2891 1166
Size: 1725 3919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2174 1184
Size: 990 4654
AlwNI CAG_nnn'CTG
Cuts at: 2161 3387 4959 2161
Size: 1226 1572 2846
Fragments arranged by size:
2846 1572 1226
ApaI G_GGCC'C
Cuts at: 2317 2317
Size: 5644
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2394 4198 160
Size: 872 1362 1804 1606
Fragments arranged by size:
1804 1606 1362 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2457 2694 2969 1870
Size: 73 514 237 275 4545
Fragments arranged by size:
4545 514 275 237 73
BamHI G'GATC_C
Cuts at: 2186 2974 3341 2186
Size: 788 367 4489
Fragments arranged by size:
4489 788 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2916 2965 4420 618
Size: 325 217 627 1129 49 1455 1842
Fragments arranged by size:
1842 1455 1129 627 325 217 49
BanII G_rGCy'C
Cuts at: 1391 2317 2458 2574 2769 2822 2963 1391
Size: 926 141 116 195 53 141 4072
Fragments arranged by size:
4072 926 195 141 141 116 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2080 928
Size: 971 181 4492
Fragments arranged by size:
4492 971 181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2200 3536 4746 2200
Size: 1336 1210 3098
Fragments arranged by size:
3098 1336 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4255 136
Size: 107 122 71 631 605 337 2246 1525
Fragments arranged by size:
2246 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2317 3705 4861 1790
Size: 527 1388 1156 2573
Fragments arranged by size:
2573 1388 1156 527
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2269 2340 3375
Size: 416 53 83 399 24 852 71 1035
Cuts at: 3375 4333 442
Size: 958 1753
Fragments arranged by size:
1753 1035 958 852 416 399 83 71
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2700 2566
Size: 134 5510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 4304 1239
Size: 1430 1635 2579
Fragments arranged by size:
2579 1635 1430
Bpu10I CC'TnA_GC
Cuts at: 2909 2909
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2684 4307 882
Size: 617 1185 1623 2219
Fragments arranged by size:
2219 1623 1185 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2510 2540 1178
Size: 30 165 30 1107 30 4282
Fragments arranged by size:
4282 1107 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2849 1186
Size: 1009 654 3981
Fragments arranged by size:
3981 1009 654
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2574 3705 3790 4861 2574
Size: 1131 85 1071 3357
Fragments arranged by size:
3357 1131 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
BsmBI CGTCTCn'nnnn_
Cuts at: 2530 2530
Size: 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 2458 2574 2769 2822 2963 3705
Size: 399 527 141 116 195 53 141 742
Cuts at: 3705 3790 4861 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 527 399 195 141 141
116 85 53
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2146 2746 2999 844
Size: 1286 16 600 253 3489
Fragments arranged by size:
3489 1286 600 253 16
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2913 4288 4475 1700
Size: 110 1103 1375 187 2869
Fragments arranged by size:
2869 1375 1103 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2236 2137
Size: 99 5545
BstYI r'GATC_y
Cuts at: 1972 2186 2974 3341 3726 3743 5195 5281 5293
Size: 214 788 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 788 385 367 346 214 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2685 2685
Size: 5644
BtgI C'CryG_G
Cuts at: 513 1065 2004 2442 513
Size: 552 939 438 3715
Fragments arranged by size:
3715 939 552 438
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 3091 3980
Size: 54 1425 50 275 18 297 964 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 964 889 297 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2572 2572
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2669 3707 4304 5091 5359 1239
Size: 393 551 486 1038 597 787 268 1524
Fragments arranged by size:
1524 1038 787 597 551 486 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2314 2314
Size: 5644
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2395 2444 2104
Size: 291 49 5304
Fragments arranged by size:
5304 291 49
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 4791 1678
Size: 3113 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2769 2801 4285
Size: 32 135 32 599 32 566 32 1484
Cuts at: 4285 4317 4359 4391 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1484 599 566 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 2988 3234 669
Size: 988 281 535 515 246 3079
Fragments arranged by size:
3079 988 535 515 281 246
HindIII A'AGCT_T
Cuts at: 748 2666 748
Size: 1918 3726
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2920 2969 2920
Size: 49 5595
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2129 10
Size: 54 1425 343 297 3525
Fragments arranged by size:
3525 1425 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2156 2891 3602 3961 4120 518
Size: 648 990 735 711 359 159 2042
Fragments arranged by size:
2042 990 735 711 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2203 2933 3060 3737 4885 5130 1606
Size: 531 66 730 127 677 1148 245 2120
Fragments arranged by size:
2120 1148 730 677 531 245 127 66
NcoI C'CATG_G
Cuts at: 513 1065 2004 2442 513
Size: 552 939 438 3715
Fragments arranged by size:
3715 939 552 438
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2155 2437 2479 2662 2804 3002 4547
Size: 97 926 282 42 183 142 198 1545
Cuts at: 4547 1132
Size: 2229
Fragments arranged by size:
2229 1545 926 282 198 183 142 97
42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 2475 4543 2475
Size: 2068 3576
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2361 1108
Size: 1253 4391
PfoI T'CCnGG_A
Cuts at: 2813 2813
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2292 2324 3713 3745 5226 5258 2292
Size: 32 1389 32 1481 32 2678
Fragments arranged by size:
2678 1481 1389 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2313 2313
Size: 5644
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2027 2059 2027
Size: 32 5612
PstI C_TGCA'G
Cuts at: 830 2226 2996 830
Size: 1396 770 3478
Fragments arranged by size:
3478 1396 770
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2137 2137
Size: 5644
SacI G_AGCT'C
Cuts at: 2574 2574
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SexAI A'CCwGG_T
Cuts at: 2403 2403
Size: 5644
SfcI C'TryA_G
Cuts at: 826 1045 2222 2992 4127 4808 4999 826
Size: 219 1177 770 1135 681 191 1471
Fragments arranged by size:
1471 1177 1135 770 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2459 2696 2971 1872
Size: 587 237 275 4545
Fragments arranged by size:
4545 587 275 237
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3748 4649 4911 820
Size: 197 926 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 2155 2662 2804 3002 1132
Size: 1023 507 142 198 3774
Fragments arranged by size:
3774 1023 507 198 142
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 2307 2546 1517
Size: 790 239 4615
Fragments arranged by size:
4615 790 239
StyI C'CwwG_G
Cuts at: 513 1065 2004 2370 2442 2924 3083 513
Size: 552 939 366 72 482 159 3074
Fragments arranged by size:
3074 939 552 482 366 159 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2269 3671 3688 3841 4026 4471 325
Size: 1255 689 1402 17 153 185 445 1498
Fragments arranged by size:
1498 1402 1255 689 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3890 5602 96
Size: 274 80 33 51 1443 1913 1712 138
Fragments arranged by size:
1913 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 2939 1316
Size: 1623 4021
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2457 2694 2969 1870
Size: 587 237 275 4545
Fragments arranged by size:
4545 587 275 237
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI