Restriction map for
pFN21AE2731
(Circular) (Six-base) MAPSORT of: pFN21AE2731.seq Check: 3755 from: 1 to: 5677
pFN21AE2731 5677 bp
With 182 enzymes: SgfI *
August 17, 2018 20:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 3020 1937
Size: 834 249 4594
Fragments arranged by size:
4594 834 249
Acc65I G'GTAC_C
Cuts at: 2796 2998 2796
Size: 202 5475
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 3740 5124 5392 1632
Size: 402 1706 1384 268 1917
Fragments arranged by size:
1917 1706 1384 402 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 3420 4992 3420
Size: 1572 4105
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2273 2424 2986 3212 3364 5473 1493
Size: 780 151 562 226 152 2109 1697
Fragments arranged by size:
2109 1697 780 562 226 152 151
AseI AT'TA_AT
Cuts at: 160 1032 2936 4231 160
Size: 872 1904 1295 1606
Fragments arranged by size:
1904 1606 1295 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 3002 1870
Size: 73 1059 4545
Fragments arranged by size:
4545 1059 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2796 2998 4453 618
Size: 325 217 627 1009 202 1455 1842
Fragments arranged by size:
1842 1455 1009 627 325 217 202
BanII G_rGCy'C
Cuts at: 1391 2996 1391
Size: 1605 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2087 928
Size: 971 188 4518
Fragments arranged by size:
4518 971 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3868 3902 1903
Size: 34 50 34 1847 34 3678
Fragments arranged by size:
3678 1847 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2879 3569 4779 2879
Size: 690 1210 3777
Fragments arranged by size:
3777 1210 690
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 3738 4894 1790
Size: 1948 1156 2573
Fragments arranged by size:
2573 1948 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 2828 3408
Size: 416 53 83 399 24 843 568 580
Cuts at: 3408 4366 442
Size: 958 1753
Fragments arranged by size:
1753 958 843 580 568 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2364 4337 1239
Size: 1125 1973 2579
Fragments arranged by size:
2579 1973 1125
Bpu10I CC'TnA_GC
Cuts at: 2121 2121
Size: 5677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3484 3866 4501 275
Size: 53 83 186 936 1951 382 635 1451
Fragments arranged by size:
1951 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2337 2367 1178
Size: 30 165 30 934 30 4488
Fragments arranged by size:
4488 934 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2148 1186
Size: 962 4715
BseYI C'CCAG_C
Cuts at: 4880 5376 4880
Size: 496 5181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2768 3127 3888 4916 1540
Size: 275 186 767 359 761 1028 2301
Fragments arranged by size:
2301 1028 767 761 359 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2996 3738 3823 4894 1391
Size: 399 1206 742 85 1071 2174
Fragments arranged by size:
2174 1206 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2772 2772
Size: 5677
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 3987 4007 3193
Size: 794 20 4863
Fragments arranged by size:
4863 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2765 3124 4013
Size: 54 1425 50 275 18 935 359 889
Cuts at: 4013 5642 8
Size: 1629 43
Fragments arranged by size:
1629 1425 935 889 359 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2765 3124 1537
Size: 275 953 359 4090
Fragments arranged by size:
4090 953 359 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2888 3607 1353
Size: 1535 719 3423
Fragments arranged by size:
3423 1535 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2364 3740 4337 5124 5392 1239
Size: 393 402 330 1376 597 787 268 1524
Fragments arranged by size:
1524 1376 787 597 402 393 330 268
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
EcoRV GAT'ATC
Cuts at: 2281 2281
Size: 5677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2674 2410
Size: 264 5413
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 4824 1678
Size: 3146 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4318 4350 4392 4424 1373
Size: 32 135 32 2746 32 42 32 2626
Fragments arranged by size:
2746 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 2800 3002 2800
Size: 202 5475
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4791 4975 1305
Size: 3486 184 2007
Fragments arranged by size:
3486 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3855
Fragments arranged by size:
3855 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2589 3635 3994 4153 518
Size: 648 1423 1046 359 159 2042
Fragments arranged by size:
2042 1423 1046 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 3093 3770 4918 5163 1606
Size: 507 980 677 1148 245 2120
Fragments arranged by size:
2120 1148 980 677 507 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5125
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 3035 4580 1132
Size: 97 1806 1545 2229
Fragments arranged by size:
2229 1806 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PstI C_TGCA'G
Cuts at: 830 3029 830
Size: 2199 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 3025 4160 4841 5032 826
Size: 219 1980 1135 681 191 1471
Fragments arranged by size:
1980 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3781 4682 4944 820
Size: 197 926 1838 901 262 1553
Fragments arranged by size:
1838 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 2047 3601 5
Size: 47 1995 1554 2081
Fragments arranged by size:
2081 1995 1554 47
StuI AGG'CCT
Cuts at: 1517 2200 1517
Size: 683 4994
StyI C'CwwG_G
Cuts at: 513 1065 2872 3116 513
Size: 552 1807 244 3074
Fragments arranged by size:
3074 1807 552 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3704 3721 3874 4059 4504 325
Size: 1255 2124 17 153 185 445 1498
Fragments arranged by size:
2124 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3923 5635 96
Size: 274 80 33 51 1443 1946 1712 138
Fragments arranged by size:
1946 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 2047 2394 3806 2047
Size: 347 1412 3918
Fragments arranged by size:
3918 1412 347
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI