Restriction map for
pFN21AE2726
(Circular) (Six-base) MAPSORT of: pFN21AE2726.seq Check: 3273 from: 1 to: 5971
pFN21AE2726 5971 bp
With 182 enzymes: SgfI *
July 3, 2018 16:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2804 2804
Size: 5971
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3212 4034 5418 5686 1632
Size: 1580 822 1384 268 1917
Fragments arranged by size:
1917 1580 1384 822 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 2276 1166
Size: 1110 4861
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 2415 3121 3714 5286 2415
Size: 706 593 1572 3100
Fragments arranged by size:
3100 1572 706 593
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2535 3008 3280 3506 3658 5767 1493
Size: 1042 473 272 226 152 2109 1697
Fragments arranged by size:
2109 1697 1042 473 272 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2154 4525 160
Size: 872 1122 2371 1606
Fragments arranged by size:
2371 1606 1122 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2130 3296 1870
Size: 73 187 1166 4545
Fragments arranged by size:
4545 1166 187 73
BamHI G'GATC_C
Cuts at: 2059 2819 2868 3301 3668 2059
Size: 760 49 433 367 4362
Fragments arranged by size:
4362 760 433 367 49
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2582 3292 4747 618
Size: 325 217 627 257 538 710 1455 1842
Fragments arranged by size:
1842 1455 710 627 538 325 257 217
BanII G_rGCy'C
Cuts at: 1391 3290 1391
Size: 1899 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2177 3200 928
Size: 971 278 1023 3699
Fragments arranged by size:
3699 1023 971 278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 2211 1548
Size: 663 5308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 2752 4032 5188 1790
Size: 962 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 962
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2425 2540 3702
Size: 416 53 83 399 24 1008 115 1162
Cuts at: 3702 4660 442
Size: 958 1753
Fragments arranged by size:
1753 1162 1008 958 416 399 115 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2939
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2583 2764 4631 1239
Size: 1344 181 1867 2579
Fragments arranged by size:
2579 1867 1344 181
Bpu10I CC'TnA_GC
Cuts at: 2903 2903
Size: 5971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2182 4634 882
Size: 617 683 2452 2219
Fragments arranged by size:
2452 2219 683 617
BsaAI yAC'GTr
Cuts at: 493 1204 3762 493
Size: 711 2558 2702
Fragments arranged by size:
2702 2558 711
BsaBI GATnn'nnATC
Cuts at: 2657 5965 2657
Size: 3308 2663
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2757 3224 3778 4160
Size: 53 83 186 936 1224 467 554 382
Cuts at: 4160 4795 275
Size: 635 1451
Fragments arranged by size:
1451 1224 936 635 554 467 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2211 2241 1178
Size: 30 165 30 808 30 4908
Fragments arranged by size:
4908 808 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5971
BseYI C'CCAG_C
Cuts at: 5174 5670 5174
Size: 496 5475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2974 4032 4117 5188 2974
Size: 1058 85 1071 3757
Fragments arranged by size:
3757 1071 1058 85
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2752 2974 3290 4032 4117 5188 1391
Size: 399 962 222 316 742 85 1071 2174
Fragments arranged by size:
2174 1071 962 742 399 316 222 85
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 2251 3860 5590 2251
Size: 1609 1730 2632
Fragments arranged by size:
2632 1730 1609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 3326 844
Size: 1960 522 3489
Fragments arranged by size:
3489 1960 522
BsrBI CCG'CTC
Cuts at: 1394 1823 2075 3858 1394
Size: 429 252 1783 3507
Fragments arranged by size:
3507 1783 429 252
BsrDI GCAATG_nn'
Cuts at: 65 1248 4466 4640 65
Size: 1183 3218 174 1396
Fragments arranged by size:
3218 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4615 4802 1700
Size: 110 2805 187 2869
Fragments arranged by size:
2869 2805 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 2016 4031 5043 2016
Size: 2015 1012 2944
Fragments arranged by size:
2944 2015 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2369 2978 794
Size: 1575 609 3787
Fragments arranged by size:
3787 1575 609
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 2059 2819 2868 3301 3668 4053 4070 5522
Size: 87 760 49 433 367 385 17 1452
Cuts at: 5522 5608 5620 5966 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 760 433 385 367 346 87
86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 3487 4281 4301 3487
Size: 794 20 5157
Fragments arranged by size:
5157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2104 2139 529
Size: 35 1540 35 4361
Fragments arranged by size:
4361 1540 35 35
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DraIII CAC_nnn'GTG
Cuts at: 3233 3233
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936
Size: 54 1425 50 275 18 1588 889 1629
Cuts at: 5936 8
Size: 43
Fragments arranged by size:
1629 1588 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2946 3237 3901 1353
Size: 1593 291 664 3423
Fragments arranged by size:
3423 1593 664 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2583 2764 3212 4034 4631 5418 5686
Size: 393 951 181 448 822 597 787 268
Cuts at: 5686 1239
Size: 1524
Fragments arranged by size:
1524 951 822 787 597 448 393 268
181
EcoO109I rG'GnC_Cy
Cuts at: 3138 3138
Size: 5971
EcoRI G'AATT_C
Cuts at: 2535 3008 3280 3658 2535
Size: 473 272 378 4848
Fragments arranged by size:
4848 473 378 272
EcoRV GAT'ATC
Cuts at: 2934 2934
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 5118 1678
Size: 3440 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 3193 3225 4612
Size: 32 135 32 479 32 1110 32 1387
Cuts at: 4612 4644 4686 4718 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1387 1110 479 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2548 5085 5269 1305
Size: 1243 2537 184 2007
Fragments arranged by size:
2537 2007 1243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 2313 3929 4288 4447 518
Size: 648 1110 37 1616 359 159 2042
Fragments arranged by size:
2042 1616 1110 648 359 159 37
MspA1I CmG'CkG
Cuts at: 1606 3061 3387 4064 5212 5457 1606
Size: 1455 326 677 1148 245 2120
Fragments arranged by size:
2120 1455 1148 677 326 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5419
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3329 4874 1132
Size: 97 2100 1545 2229
Fragments arranged by size:
2229 2100 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3138 3138
Size: 5971
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 3323 830
Size: 2493 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 3319 4454 5135 5326 826
Size: 219 2274 1135 681 191 1471
Fragments arranged by size:
2274 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 2132 3298 1872
Size: 260 1166 4545
Fragments arranged by size:
4545 1166 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4075 4976 5238 820
Size: 197 926 2132 901 262 1553
Fragments arranged by size:
2132 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 2508 3895 5
Size: 47 2456 1387 2081
Fragments arranged by size:
2456 2081 1387 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 3410 513
Size: 552 2345 3074
Fragments arranged by size:
3074 2345 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3998 4015 4168 4353 4798 325
Size: 1255 2418 17 153 185 445 1498
Fragments arranged by size:
2418 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2695 4217 5929
Size: 274 80 33 51 1443 718 1522 1712
Cuts at: 5929 96
Size: 138
Fragments arranged by size:
1712 1522 1443 718 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2211 2243 5931 5963 2211
Size: 32 3688 32 2219
Fragments arranged by size:
3688 2219 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3142 1316
Size: 1826 4145
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2188 2767 2188
Size: 579 5392
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 2130 3296 1870
Size: 260 1166 4545
Fragments arranged by size:
4545 1166 260
XmnI GAAnn'nnTTC
Cuts at: 2343 4100 2343
Size: 1757 4214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI