(Circular) (Six-base) MAPSORT of: pFN21AE2725.seq Check: 9581 from: 1 to: 5914
pFN21AE2725 5914 bp
With 182 enzymes: SgfI *
October 2, 2018 19:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2985 3257 1937
Size: 1048 272 4594
Fragments arranged by size:
4594 1048 272
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2311 2891 3977 5361 5629 1632
Size: 679 580 1086 1384 268 1917
Fragments arranged by size:
1917 1384 1086 679 580 268
AfeI AGC'GCT
Cuts at: 2674 2674
Size: 5914
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 2944 3030 4813 5790 1203
Size: 1741 86 1783 977 1327
Fragments arranged by size:
1783 1741 1327 977 86
AgeI A'CCGG_T
Cuts at: 2110 4745 2110
Size: 2635 3279
AhdI GACnn_n'nnGTC
Cuts at: 3100 4643 3100
Size: 1543 4371
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2219 1184
Size: 1035 4879
AlwNI CAG_nnn'CTG
Cuts at: 2305 2416 2974 3657 5229 2305
Size: 111 558 683 1572 2990
Fragments arranged by size:
2990 1572 683 558 111
ApaI G_GGCC'C
Cuts at: 2480 2540 2480
Size: 60 5854
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 3223 3449 3601 5710 1493
Size: 1730 226 152 2109 1697
Fragments arranged by size:
2109 1730 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2598 2598
Size: 5914
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 2236 2242 2312 2333 3085 3110 3239
Size: 73 293 6 70 21 752 25 129
Cuts at: 3239 1870
Size: 4545
Fragments arranged by size:
4545 752 293 129 73 70 25 21
6
BamHI G'GATC_C
Cuts at: 3244 3611 3244
Size: 367 5547
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2203 2227 2438 2444 2606
Size: 325 217 627 416 24 211 6 162
Cuts at: 2606 2735 3235 4690 618
Size: 129 500 1455 1842
Fragments arranged by size:
1842 1455 627 500 416 325 217 211
162 129 24 6
BanII G_rGCy'C
Cuts at: 1391 2165 2243 2480 2540 3132 3233 1391
Size: 774 78 237 60 592 101 4072
Fragments arranged by size:
4072 774 592 237 101 78 60
BbeI G_GCGC'C
Cuts at: 2231 2448 2610 2739 2231
Size: 217 162 129 5406
Fragments arranged by size:
5406 217 162 129
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2277 928
Size: 971 378 4565
Fragments arranged by size:
4565 971 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4105 4139 1903
Size: 34 2168 34 3678
Fragments arranged by size:
3678 2168 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3152 3806 5016 3152
Size: 654 1210 4050
Fragments arranged by size:
4050 1210 654
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 2426 2630
Size: 107 122 71 631 605 470 284 204
Cuts at: 2630 4525 136
Size: 1895 1525
Fragments arranged by size:
1895 1525 631 605 470 284 204 122
107 71
BglII A'GATC_T
Cuts at: 3003 5909 3003
Size: 2906 3008
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 2208 2480 2540 3975 5131 1790
Size: 418 272 60 1435 1156 2573
Fragments arranged by size:
2573 1435 1156 418 272 60
BmgBI CAC'GTC
Cuts at: 1318 2947 1318
Size: 1629 4285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2905 3645 4603
Size: 416 53 83 399 24 1488 740 958
Cuts at: 4603 442
Size: 1753
Fragments arranged by size:
1753 1488 958 740 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2972 2992 4574 1239
Size: 1121 612 20 1582 2579
Fragments arranged by size:
2579 1582 1121 612 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2978 4039 4904 5202 2978
Size: 1061 865 298 3690
Fragments arranged by size:
3690 1061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2111 4577 882
Size: 617 612 2466 2219
Fragments arranged by size:
2466 2219 617 612
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 5908 5908
Size: 5914
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2228 2445 2607 2736
Size: 53 83 186 936 695 217 162 129
Cuts at: 2736 3721 4103 4738 275
Size: 985 382 635 1451
Fragments arranged by size:
1451 985 936 695 635 382 217 186
162 129 83 53
BsaWI w'CCGG_w
Cuts at: 2110 4347 4745 5019 5166 5577 2110
Size: 2237 398 274 147 411 2447
Fragments arranged by size:
2447 2237 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2771 2801 1178
Size: 30 165 30 1368 30 4291
Fragments arranged by size:
4291 1368 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 2870 1186
Size: 1297 387 4230
Fragments arranged by size:
4230 1297 387
BseYI C'CCAG_C
Cuts at: 2216 2231 2320 2642 2675 3180 5117 5613 2216
Size: 15 89 322 33 505 1937 496 2517
Fragments arranged by size:
2517 1937 505 496 322 89 33 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2215 2726 2753 2954 1163
Size: 573 479 511 27 201 4123
Fragments arranged by size:
4123 573 511 479 201 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2125 2146 2346 2430 2517 2595
Size: 275 186 124 21 200 84 87 78
Cuts at: 2595 2625 2634 2782 3364 4125 5153 1540
Size: 30 9 148 582 761 1028 2301
Fragments arranged by size:
2301 1028 761 582 275 200 186 148
124 87 84 78 30 21 9
BsiHKAI G_wGCw'C
Cuts at: 2189 3975 4060 5131 2189
Size: 1786 85 1071 2972
Fragments arranged by size:
2972 1786 1071 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
BsmBI CGTCTCn'nnnn_
Cuts at: 2153 2153
Size: 5914
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2189 2208 2243 2480 2540 3132
Size: 399 375 24 19 35 237 60 592
Cuts at: 3132 3233 3975 4060 5131 1391
Size: 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 592 399 375 237 101
85 60 35 24 19
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 3269 844
Size: 1202 1223 3489
Fragments arranged by size:
3489 1223 1202
BsrBI CCG'CTC
Cuts at: 1394 1823 2529 3801 1394
Size: 429 706 1272 3507
Fragments arranged by size:
3507 1272 706 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2028 4409 4583 65
Size: 1183 780 2381 174 1396
Fragments arranged by size:
2381 1396 1183 780 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2110 2288 2435 2441 2570 2594 2633
Size: 110 300 178 147 6 129 24 39
Cuts at: 2633 2759 2829 4558 4745 1700
Size: 126 70 1729 187 2869
Fragments arranged by size:
2869 1729 300 187 178 147 129 126
110 70 39 24 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 2399 2598 1925
Size: 474 199 5241
Fragments arranged by size:
5241 474 199
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2193 794
Size: 1399 4515
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 2803 1184
Size: 1619 4295
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 2053
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 3003 3244 3611 3996 4013 5465 5551 5563
Size: 1031 241 367 385 17 1452 86 12
Cuts at: 5563 5909 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1031 385 367 346 241 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 2421 2490 2511 2610 2899
Size: 552 939 54 363 69 21 99 289
Cuts at: 2899 513
Size: 3528
Fragments arranged by size:
3528 939 552 363 289 99 69 54
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2137 487
Size: 683 56 911 4264
Fragments arranged by size:
4264 911 683 56
BtsI GCAGTG_nn'
Cuts at: 3430 4224 4244 3430
Size: 794 20 5100
Fragments arranged by size:
5100 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 3218 3218
Size: 5914
DraIII CAC_nnn'GTG
Cuts at: 2193 2193
Size: 5914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4921 809
Size: 507 3605 1802
Fragments arranged by size:
3605 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2143 2418
Size: 54 1425 50 275 18 225 88 275
Cuts at: 2418 2427 2514 2572 2592 2622 2631 2667 3361
Size: 9 87 58 20 30 9 36 694
Cuts at: 3361 4250 5879 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 694 275 275 225 88
87 58 54 50 43 36 30 20
18 9 9
EagI C'GGCC_G
Cuts at: 1537 1812 2143 2427 2514 2592 2622 2631 3361
Size: 275 331 284 87 78 30 9 730
Cuts at: 3361 1537
Size: 4090
Fragments arranged by size:
4090 730 331 284 275 87 78 30
9
EarI CTCTTCn'nnn_
Cuts at: 1353 2873 3844 1353
Size: 1520 971 3423
Fragments arranged by size:
3423 1520 971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2488 2718 2899 4501 4875 5021
Size: 1064 138 899 230 181 1602 374 146
Cuts at: 5021 387
Size: 1280
Fragments arranged by size:
1602 1280 1064 899 374 230 181 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2311 2360 2891 2972 2992 3977 4574
Size: 393 679 49 531 81 20 985 597
Cuts at: 4574 5361 5629 1239
Size: 787 268 1524
Fragments arranged by size:
1524 985 787 679 597 531 393 268
81 49 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2996 2996
Size: 5914
EcoO109I rG'GnC_Cy
Cuts at: 2477 2477
Size: 5914
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 2231 2321 2354 2448 2610 2643 2676 2739
Size: 553 90 33 94 162 33 33 63
Cuts at: 2739 5061 1678
Size: 2322 2531
Fragments arranged by size:
2531 2322 553 162 94 90 63 33
33 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2771 2803 3086 3118 4555
Size: 32 135 32 1199 32 283 32 1437
Cuts at: 4555 4587 4629 4661 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1437 1199 283 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 3258 3504 669
Size: 988 281 1048 272 246 3079
Fragments arranged by size:
3079 1048 988 281 272 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KasI G'GCGC_C
Cuts at: 2227 2444 2606 2735 2227
Size: 217 162 129 5406
Fragments arranged by size:
5406 217 162 129
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 3030 5790 3030
Size: 2760 3154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 2814 2937 3123 5028 5212 1305
Size: 753 756 123 186 1905 184 2007
Fragments arranged by size:
2007 1905 756 753 186 184 123
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 10
Size: 54 1425 343 225 3867
Fragments arranged by size:
3867 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2813 3872 4231 4390 518
Size: 648 1014 633 1059 359 159 2042
Fragments arranged by size:
2042 1059 1014 648 633 359 159
MspA1I CmG'CkG
Cuts at: 1606 2305 2416 2423 2463 2513 2612 2800 2836
Size: 699 111 7 40 50 99 188 36
Cuts at: 2836 2901 2971 3066 3330 4007 5155 5400 1606
Size: 65 70 95 264 677 1148 245 2120
Fragments arranged by size:
2120 1148 699 677 264 245 188 111
99 95 70 65 50 40 36 7
NaeI GCC'GGC
Cuts at: 2443 2572 2596 2761 2443
Size: 129 24 165 5596
Fragments arranged by size:
5596 165 129 24
NarI GG'CG_CC
Cuts at: 2228 2445 2607 2736 2228
Size: 217 162 129 5406
Fragments arranged by size:
5406 217 162 129
NcoI C'CATG_G
Cuts at: 513 1065 2004 2058 513
Size: 552 939 54 4369
Fragments arranged by size:
4369 939 552 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NgoMIV G'CCGG_C
Cuts at: 2441 2570 2594 2759 2441
Size: 129 24 165 5596
Fragments arranged by size:
5596 165 129 24
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 2514 2622 3361 2514
Size: 108 739 5067
Fragments arranged by size:
5067 739 108
NspI r_CATG'y
Cuts at: 1132 1229 3272 4817 1132
Size: 97 2043 1545 2229
Fragments arranged by size:
2229 2043 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5914
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3171 1108
Size: 2063 3851
PfoI T'CCnGG_A
Cuts at: 2949 2949
Size: 5914
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PspOMI G'GGCC_C
Cuts at: 2476 2536 2476
Size: 60 5854
PspXI vC'TCGA_Gb
Cuts at: 3085 3085
Size: 5914
PstI C_TGCA'G
Cuts at: 830 2979 3266 830
Size: 2149 287 3478
Fragments arranged by size:
3478 2149 287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
PvuII CAG'CTG
Cuts at: 2305 2416 2800 2836 2971 2305
Size: 111 384 36 135 5248
Fragments arranged by size:
5248 384 135 111 36
SacII CC_GC'GG
Cuts at: 2424 2514 2613 2902 2424
Size: 90 99 289 5436
Fragments arranged by size:
5436 289 99 90
SalI G'TCGA_C
Cuts at: 1936 2984 3256 1936
Size: 1048 272 4594
Fragments arranged by size:
4594 1048 272
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SfcI C'TryA_G
Cuts at: 826 1045 2975 3262 4397 5078 5269 826
Size: 219 1930 287 1135 681 191 1471
Fragments arranged by size:
1930 1471 1135 681 287 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2426 2630 2426
Size: 204 5710
SfoI GGC'GCC
Cuts at: 2229 2446 2608 2737 2229
Size: 217 162 129 5406
Fragments arranged by size:
5406 217 162 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 2759 1700
Size: 1059 4855
SmaI CCC'GGG
Cuts at: 1872 2238 3241 1872
Size: 366 1003 4545
Fragments arranged by size:
4545 1003 366
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2993 3085 4018 4919 5181 820
Size: 197 926 1050 92 933 901 262 1553
Fragments arranged by size:
1553 1050 933 926 901 262 197 92
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 3272 1132
Size: 2140 3774
SrfI GCCC'GGGC
Cuts at: 2238 2238
Size: 5914
SspI AAT'ATT
Cuts at: 5 52 3838 5
Size: 47 3786 2081
Fragments arranged by size:
3786 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5914
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 2451 3353 513
Size: 552 939 54 393 902 3074
Fragments arranged by size:
3074 939 902 552 393 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 3941 3958 4111 4296 4741 325
Size: 1255 555 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 555 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3190 4160 5872
Size: 274 80 33 51 1443 1213 970 1712
Cuts at: 5872 96
Size: 138
Fragments arranged by size:
1712 1443 1213 970 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5874 5906 5874
Size: 32 5882
Tth111I GACn'n_nGTC
Cuts at: 1316 2342 2809 1316
Size: 1026 467 4421
Fragments arranged by size:
4421 1026 467
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 3139 2050
Size: 1089 4825
XhoI C'TCGA_G
Cuts at: 1943 3085 1943
Size: 1142 4772
XmaI C'CCGG_G
Cuts at: 1870 2236 3239 1870
Size: 366 1003 4545
Fragments arranged by size:
4545 1003 366
XmnI GAAnn'nnTTC
Cuts at: 4043 4043
Size: 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PpuMI PsrI RsrII SacI
SanDI SapI SexAI SwaI