Restriction map for
pFN21AE2723
(Circular) (Six-base) MAPSORT of: pFN21AE2723.seq Check: 8435 from: 1 to: 5611
pFN21AE2723 5611 bp
With 182 enzymes: SgfI *
July 7, 2018 16:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2891 2891
Size: 5611
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2954 1937
Size: 1017 4594
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 2747 3674 5058 5326 1632
Size: 620 495 927 1384 268 1917
Fragments arranged by size:
1917 1384 927 620 495 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5414
AflIII A'CryG_T
Cuts at: 1203 4510 5487 1203
Size: 3307 977 1327
Fragments arranged by size:
3307 1327 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 2651 1166
Size: 1485 4126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 3354 4926 3354
Size: 1572 4039
ApaLI G'TGCA_C
Cuts at: 3668 4824 3668
Size: 1156 4455
ApoI r'AATT_y
Cuts at: 1493 2864 2920 3146 3298 5407 1493
Size: 1371 56 226 152 2109 1697
Fragments arranged by size:
2109 1697 1371 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2849 2936 1870
Size: 73 906 87 4545
Fragments arranged by size:
4545 906 87 73
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2189 2555 2932 4387 618
Size: 325 217 627 402 366 377 1455 1842
Fragments arranged by size:
1842 1455 627 402 377 366 325 217
BanII G_rGCy'C
Cuts at: 1391 2930 1391
Size: 1539 4072
BbeI G_GCGC'C
Cuts at: 2193 2193
Size: 5611
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3802 3836 1903
Size: 34 1865 34 3678
Fragments arranged by size:
3678 1865 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3503 4713 3503
Size: 1210 4401
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5611
BfrBI ATG'CAT
Cuts at: 2806 2806
Size: 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4222 136
Size: 107 122 71 631 605 2550 1525
Fragments arranged by size:
2550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5606 5606
Size: 5611
BlpI GC'TnA_GC
Cuts at: 3028 3028
Size: 5611
Bme1580I G_kGCm'C
Cuts at: 1790 2267 3672 4828 1790
Size: 477 1405 1156 2573
Fragments arranged by size:
2573 1405 1156 477
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5611
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3342 4300 442
Size: 416 53 83 399 24 1925 958 1753
Fragments arranged by size:
1925 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2513 4271 1239
Size: 1274 1758 2579
Fragments arranged by size:
2579 1758 1274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 3736 4601 4899 2411
Size: 1325 865 298 3123
Fragments arranged by size:
3123 1325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4274 882
Size: 617 2775 2219
Fragments arranged by size:
2775 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3402 493
Size: 711 2198 2702
Fragments arranged by size:
2702 2198 711
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2158 2190 3418 3800
Size: 53 83 186 936 625 32 1228 382
Cuts at: 3800 4435 275
Size: 635 1451
Fragments arranged by size:
1451 1228 936 635 625 382 186 83
53 32
BsaWI w'CCGG_w
Cuts at: 4044 4442 4716 4863 5274 4044
Size: 398 274 147 411 4381
Fragments arranged by size:
4381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2297 2327 1178
Size: 30 165 30 894 30 4462
Fragments arranged by size:
4462 894 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2321 2414 2774 2777 2780 2867 1186
Size: 1075 60 93 360 3 3 87 3930
Fragments arranged by size:
3930 1075 360 93 87 60 3 3
BseYI C'CCAG_C
Cuts at: 2020 4814 5310 2020
Size: 2794 496 2321
Fragments arranged by size:
2794 2321 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2554 2786 2829 1163
Size: 573 818 232 43 3945
Fragments arranged by size:
3945 818 573 232 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3061 3822 4850 1540
Size: 275 186 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3672 3757 4828 3672
Size: 85 1071 4455
Fragments arranged by size:
4455 1071 85
BsmI GAATG_Cn'
Cuts at: 3122 3215 3122
Size: 93 5518
BsmBI CGTCTCn'nnnn_
Cuts at: 2231 2729 2231
Size: 498 5113
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2267 2930 3672 3757 4828 1391
Size: 399 477 663 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 663 477 399 85
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 2966 844
Size: 2047 75 3489
Fragments arranged by size:
3489 2047 75
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 1394
Size: 429 1675 3507
Fragments arranged by size:
3507 1675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4106 4280 65
Size: 1183 2858 174 1396
Fragments arranged by size:
2858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2135 4255 4442 1700
Size: 110 325 2120 187 2869
Fragments arranged by size:
2869 2120 325 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 2025 1925
Size: 100 5511
BssSI C'ACGA_G
Cuts at: 3671 4683 3671
Size: 1012 4599
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2271 2543 794
Size: 1477 272 3862
Fragments arranged by size:
3862 1477 272
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2250 2856 2941 3308 3693 3710 5162 5248
Size: 278 606 85 367 385 17 1452 86
Cuts at: 5248 5260 5606 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 606 385 367 346 278 86
85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2379 2386 2795 513
Size: 552 939 375 7 409 3329
Fragments arranged by size:
3329 939 552 409 375 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4872
Fragments arranged by size:
4872 683 56
BtsI GCAGTG_nn'
Cuts at: 3127 3921 3941 3127
Size: 794 20 4797
Fragments arranged by size:
4797 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DraIII CAC_nnn'GTG
Cuts at: 2271 2515 2271
Size: 244 5367
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4618 809
Size: 507 3302 1802
Fragments arranged by size:
3302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2506 3058
Size: 54 1425 50 275 18 492 184 552
Cuts at: 3058 3947 5576 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 552 492 275 184 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3058 1537
Size: 275 1246 4090
Fragments arranged by size:
4090 1246 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 3541 1353
Size: 1034 1154 3423
Fragments arranged by size:
3423 1154 1034
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2920 4198 4572 4718 387
Size: 1064 138 1331 1278 374 146 1280
Fragments arranged by size:
1331 1280 1278 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2513 2747 3674 4271 5058 5326
Size: 393 620 261 234 927 597 787 268
Cuts at: 5326 1239
Size: 1524
Fragments arranged by size:
1524 927 787 620 597 393 268 261
234
EcoO109I rG'GnC_Cy
Cuts at: 2107 2779 2107
Size: 672 4939
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 2193 2646 4758 1678
Size: 515 453 2112 2531
Fragments arranged by size:
2531 2112 515 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2041 2058 2073 4252
Size: 32 135 32 454 15 17 15 2179
Cuts at: 4252 4284 4326 4358 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2179 454 135 42 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2955 3201 669
Size: 988 281 1017 246 3079
Fragments arranged by size:
3079 1017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KasI G'GCGC_C
Cuts at: 2189 2189
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2059 4725 4909 1305
Size: 754 2666 184 2007
Fragments arranged by size:
2666 2007 754 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2508 10
Size: 54 1425 343 676 3113
Fragments arranged by size:
3113 1425 676 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2651 2678 2885 3569 3928 4087
Size: 648 1197 288 27 207 684 359 159
Cuts at: 4087 518
Size: 2042
Fragments arranged by size:
2042 1197 684 648 359 288 207 159
27
MspA1I CmG'CkG
Cuts at: 1606 3027 3704 4852 5097 1606
Size: 1421 677 1148 245 2120
Fragments arranged by size:
2120 1421 1148 677 245
NarI GG'CG_CC
Cuts at: 2190 2190
Size: 5611
NcoI C'CATG_G
Cuts at: 513 1065 2004 2386 2795 513
Size: 552 939 382 409 3329
Fragments arranged by size:
3329 939 552 409 382
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NsiI A_TGCA'T
Cuts at: 2808 2808
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2810 2969 4514 1132
Size: 97 1581 159 1545 2229
Fragments arranged by size:
2229 1581 1545 159 97
PasI CC'CwG_GG
Cuts at: 1348 2449 2614 1348
Size: 1101 165 4345
Fragments arranged by size:
4345 1101 165
PciI A'CATG_T
Cuts at: 4510 4510
Size: 5611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4709
PfoI T'CCnGG_A
Cuts at: 2334 2632 2334
Size: 298 5313
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 2107
Size: 5611
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PspXI vC'TCGA_Gb
Cuts at: 2849 2849
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2502 2963 830
Size: 1672 461 3478
Fragments arranged by size:
3478 1672 461
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SapI GCTCTTCn'nnn_
Cuts at: 2387 2387
Size: 5611
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SfcI C'TryA_G
Cuts at: 826 1045 2045 2498 2959 4094 4775 4966 826
Size: 219 1000 453 461 1135 681 191 1471
Fragments arranged by size:
1471 1135 1000 681 461 453 219 191
SfoI GGC'GCC
Cuts at: 2191 2191
Size: 5611
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 2135 1700
Size: 435 5176
SmaI CCC'GGG
Cuts at: 1872 2938 1872
Size: 1066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 2849 3715 4616 4878 820
Size: 197 926 483 423 866 901 262 1553
Fragments arranged by size:
1553 926 901 866 483 423 262 197
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2810 2969 1132
Size: 1678 159 3774
Fragments arranged by size:
3774 1678 159
SspI AAT'ATT
Cuts at: 5 52 3535 5
Size: 47 3483 2081
Fragments arranged by size:
3483 2081 47
StuI AGG'CCT
Cuts at: 1517 2474 1517
Size: 957 4654
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 2386 2469 2795 3050 513
Size: 552 939 321 61 83 326 255 3074
Fragments arranged by size:
3074 939 552 326 321 255 83 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2336 3638 3655 3808 3993 4438 325
Size: 1255 756 1302 17 153 185 445 1498
Fragments arranged by size:
1498 1302 1255 756 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3857 5569 96
Size: 274 80 33 51 1443 1880 1712 138
Fragments arranged by size:
1880 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5571 5603 5571
Size: 32 5579
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5611
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XhoI C'TCGA_G
Cuts at: 1943 2849 1943
Size: 906 4705
XmaI C'CCGG_G
Cuts at: 1870 2936 1870
Size: 1066 4545
XmnI GAAnn'nnTTC
Cuts at: 2894 3740 2894
Size: 846 4765
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspOMI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI