Restriction map for
pFN21AE2722
(Circular) (Six-base) MAPSORT of: pFN21AE2722.seq Check: 1442 from: 1 to: 5605
pFN21AE2722 5605 bp
With 182 enzymes: SgfI *
June 6, 2018 18:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2170 3415 278
Size: 53 83 186 936 634 1245 2468
Fragments arranged by size:
2468 1245 936 634 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2346 2556 2948 1937
Size: 409 210 392 4594
Fragments arranged by size:
4594 409 392 210
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2653 3668 5052 5320 1632
Size: 1021 1015 1384 268 1917
Fragments arranged by size:
1917 1384 1021 1015 268
AfeI AGC'GCT
Cuts at: 2577 2577
Size: 5605
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2429 2461 2429
Size: 32 5573
AlwNI CAG_nnn'CTG
Cuts at: 2473 3348 4920 2473
Size: 875 1572 3158
Fragments arranged by size:
3158 1572 875
ApaI G_GGCC'C
Cuts at: 2188 2188
Size: 5605
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2914 3140 3292 5401 1493
Size: 1421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2175 2930 1870
Size: 73 79 153 755 4545
Fragments arranged by size:
4545 755 153 79 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 4381 618
Size: 325 217 627 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2188 2924 1391
Size: 797 736 4072
Fragments arranged by size:
4072 797 736
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4634
BbvCI CC'TCA_GC
Cuts at: 2295 2295
Size: 5605
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3796 3830 1903
Size: 34 50 34 1775 34 3678
Fragments arranged by size:
3678 1775 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2412 5600 2412
Size: 3188 2417
BlpI GC'TnA_GC
Cuts at: 2462 3022 2462
Size: 560 5045
Bme1580I G_kGCm'C
Cuts at: 1790 2188 3666 4822 1790
Size: 398 1478 1156 2573
Fragments arranged by size:
2573 1478 1156 398
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2780 4265 1239
Size: 1109 432 1485 2579
Fragments arranged by size:
2579 1485 1109 432
Bpu10I CC'TnA_GC
Cuts at: 2295 2295
Size: 5605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2518 2591 4268 882
Size: 617 1019 73 1677 2219
Fragments arranged by size:
2219 1677 1019 617 73
BsaAI yAC'GTr
Cuts at: 493 1204 2191 2284 3396 493
Size: 711 987 93 1112 2702
Fragments arranged by size:
2702 1112 987 711 93
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 2167 2343 3412
Size: 53 83 186 936 567 67 176 1069
Cuts at: 3412 3794 4429 275
Size: 382 635 1451
Fragments arranged by size:
1451 1069 936 635 567 382 186 176
83 67 53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2875 1186
Size: 1689 3916
BseYI C'CCAG_C
Cuts at: 2449 2548 2581 4808 5304 2449
Size: 99 33 2227 496 2750
Fragments arranged by size:
2750 2227 496 99 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2380 2789 1163
Size: 573 644 409 3979
Fragments arranged by size:
3979 644 573 409
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2334 2393 2794 3666 3751 4822 2334
Size: 59 401 872 85 1071 3117
Fragments arranged by size:
3117 1071 872 401 85 59
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2334 2393 2794 2924 3666 3751
Size: 399 398 146 59 401 130 742 85
Cuts at: 3751 4822 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 401 399 398 146 130
85 59
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2960 844
Size: 2116 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2520 2825 4100 4274 65
Size: 1183 1272 305 1275 174 1396
Fragments arranged by size:
1396 1275 1272 1183 305 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4249 4436 1700
Size: 110 2439 187 2869
Fragments arranged by size:
2869 2439 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2277 794
Size: 1483 4122
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2326
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2412 2424 2935 3302 3687 3704 5156 5242
Size: 440 12 511 367 385 17 1452 86
Cuts at: 5242 5254 5600 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 511 440 385 367 346 86
17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 2121 2232 513
Size: 552 1056 111 3886
Fragments arranged by size:
3886 1056 552 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2143 487
Size: 683 56 917 3949
Fragments arranged by size:
3949 917 683 56
BtsI GCAGTG_nn'
Cuts at: 3121 3915 3935 3121
Size: 794 20 4791
Fragments arranged by size:
4791 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2316 3052
Size: 54 1425 50 275 18 405 81 736
Cuts at: 3052 3941 5570 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 736 405 275 81 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2521 3535 1353
Size: 1168 1014 3423
Fragments arranged by size:
3423 1168 1014
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2653 2780 3668 4265 5052 5320
Size: 393 716 305 127 888 597 787 268
Cuts at: 5320 1239
Size: 1524
Fragments arranged by size:
1524 888 787 716 597 393 305 268
127
EcoNI CCTnn'n_nnAGG
Cuts at: 2053 2382 2053
Size: 329 5276
EcoO109I rG'GnC_Cy
Cuts at: 2184 2323 2184
Size: 139 5466
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 2549 2579 4752 1678
Size: 871 30 2173 2531
Fragments arranged by size:
2531 2173 871 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4246 4278 4320 4352 1373
Size: 32 135 32 2674 32 42 32 2626
Fragments arranged by size:
2674 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2347 2374 2814 2949 3195 669
Size: 988 281 409 27 440 135 246 3079
Fragments arranged by size:
3079 988 440 409 281 246 135 27
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4719 4903 1305
Size: 3414 184 2007
Fragments arranged by size:
3414 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 2318 10
Size: 54 1425 343 405 81 3297
Fragments arranged by size:
3297 1425 405 343 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2049 2699 2830 3563 3922 4081 518
Size: 648 883 650 131 733 359 159 2042
Fragments arranged by size:
2042 883 733 650 648 359 159 131
MspA1I CmG'CkG
Cuts at: 1606 2311 2739 3021 3698 4846 5091 1606
Size: 705 428 282 677 1148 245 2120
Fragments arranged by size:
2120 1148 705 677 428 282 245
NcoI C'CATG_G
Cuts at: 513 1065 2232 513
Size: 552 1167 3886
Fragments arranged by size:
3886 1167 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PfoI T'CCnGG_A
Cuts at: 2647 2647
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PmlI CAC'GTG
Cuts at: 2191 2284 2191
Size: 93 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 2532 3175 2532
Size: 643 4962
PspOMI G'GGCC_C
Cuts at: 2184 2184
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2247 2744 2957 830
Size: 1417 497 213 3478
Fragments arranged by size:
3478 1417 497 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2311 2739 2311
Size: 428 5177
SalI G'TCGA_C
Cuts at: 1936 2345 2947 1936
Size: 409 602 4594
Fragments arranged by size:
4594 602 409
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2243 2740 2953 4088 4769 4960 826
Size: 219 1198 497 213 1135 681 191 1471
Fragments arranged by size:
1471 1198 1135 681 497 219 213 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2024 2177 2932 1872
Size: 152 153 755 4545
Fragments arranged by size:
4545 755 153 152
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2232 2319 2619 3044 513
Size: 552 1167 87 300 425 3074
Fragments arranged by size:
3074 1167 552 425 300 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2540 3632 3649 3802 3987 4432 325
Size: 1255 960 1092 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1092 960 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2022 2175 2930 1870
Size: 152 153 755 4545
Fragments arranged by size:
4545 755 153 152
XmnI GAAnn'nnTTC
Cuts at: 2719 3734 2719
Size: 1015 4590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2168 3413 276
Size: 53 83 186 936 634 1245 2468
Fragments arranged by size:
2468 1245 936 634 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI