Restriction map for
pFN21AE2720
(Circular) (Six-base) MAPSORT of: pFN21AE2720.seq Check: 3127 from: 1 to: 5629
pFN21AE2720 5629 bp
With 182 enzymes: SgfI *
June 6, 2018 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2522 2522
Size: 5629
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3439 278
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2688 2972 1937
Size: 751 284 4594
Fragments arranged by size:
4594 751 284
Acc65I G'GTAC_C
Cuts at: 2840 2950 2840
Size: 110 5519
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2582 3692 5076 5344 1632
Size: 950 1110 1384 268 1917
Fragments arranged by size:
1917 1384 1110 950 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 2254 4528 5505 1203
Size: 1051 2274 977 1327
Fragments arranged by size:
2274 1327 1051 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AlwNI CAG_nnn'CTG
Cuts at: 3372 4944 3372
Size: 1572 4057
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2456 2854 2913 2938 3164 3316 5425 1493
Size: 963 398 59 25 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 398 226 152 59 25
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 1870
Size: 73 1011 4545
Fragments arranged by size:
4545 1011 73
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2711 2840 2950 4405 618
Size: 325 217 627 924 129 110 1455 1842
Fragments arranged by size:
1842 1455 924 627 325 217 129 110
BanII G_rGCy'C
Cuts at: 1391 2382 2948 1391
Size: 991 566 4072
Fragments arranged by size:
4072 991 566
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 928
Size: 971 285 4373
Fragments arranged by size:
4373 971 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3820 3854 1903
Size: 34 50 34 1799 34 3678
Fragments arranged by size:
3678 1799 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2202 3521 4731 2202
Size: 1319 1210 3100
Fragments arranged by size:
3100 1319 1210
BclI T'GATC_A
Cuts at: 1548 2113 2227 1548
Size: 565 114 4950
Fragments arranged by size:
4950 565 114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4240 136
Size: 107 122 71 631 605 2568 1525
Fragments arranged by size:
2568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2560 2810 5624 2560
Size: 250 2814 2565
Fragments arranged by size:
2814 2565 250
BlpI GC'TnA_GC
Cuts at: 3046 3046
Size: 5629
Bme1580I G_kGCm'C
Cuts at: 1790 3690 4846 1790
Size: 1900 1156 2573
Fragments arranged by size:
2573 1900 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3360 4318 442
Size: 416 53 83 399 24 1943 958 1753
Fragments arranged by size:
1943 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4289 1239
Size: 3050 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2203 3754 4619 4917 2203
Size: 1551 865 298 2915
Fragments arranged by size:
2915 1551 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4292 882
Size: 617 2793 2219
Fragments arranged by size:
2793 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 5623 5623
Size: 5629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3436 3818 4453 275
Size: 53 83 186 936 1903 382 635 1451
Fragments arranged by size:
1903 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2205 4062 4460 4734 4881 5292 2205
Size: 1857 398 274 147 411 2542
Fragments arranged by size:
2542 1857 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5404
Fragments arranged by size:
5404 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2365 2368 1186
Size: 1179 3 4447
Fragments arranged by size:
4447 1179 3
BseYI C'CCAG_C
Cuts at: 2715 4832 5328 2715
Size: 2117 496 3016
Fragments arranged by size:
3016 2117 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2878 1163
Size: 573 1142 3914
Fragments arranged by size:
3914 1142 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 3840 4868 1540
Size: 275 186 1078 761 1028 2301
Fragments arranged by size:
2301 1078 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2382 3690 3775 4846 2382
Size: 1308 85 1071 3165
Fragments arranged by size:
3165 1308 1071 85
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2948 3690 3775 4846 1391
Size: 399 592 566 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 592 566 399 85
BspEI T'CCGG_A
Cuts at: 2205 5292 2205
Size: 3087 2542
BspHI T'CATG_A
Cuts at: 2338 2697 3518 5248 2338
Size: 359 821 1730 2719
Fragments arranged by size:
2719 1730 821 359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2522 2984 844
Size: 1678 462 3489
Fragments arranged by size:
3489 1678 462
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 1394
Size: 429 1693 3507
Fragments arranged by size:
3507 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4124 4298 65
Size: 1183 2876 174 1396
Fragments arranged by size:
2876 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4273 4460 1700
Size: 110 2463 187 2869
Fragments arranged by size:
2869 2463 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 3689 4701 3689
Size: 1012 4617
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5629
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2632 2143
Size: 489 5140
BstYI r'GATC_y
Cuts at: 1972 2500 2560 2810 2959 3326 3711 3728 5180
Size: 528 60 250 149 367 385 17 1452
Cuts at: 5180 5266 5278 5624 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 528 385 367 346 250 149
86 60 17 12
Bsu36I CC'TnA_GG
Cuts at: 2348 2348
Size: 5629
BtgI C'CryG_G
Cuts at: 513 1065 2012 513
Size: 552 947 4130
Fragments arranged by size:
4130 947 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4890
Fragments arranged by size:
4890 683 56
BtsI GCAGTG_nn'
Cuts at: 3145 3939 3959 3145
Size: 794 20 4815
Fragments arranged by size:
4815 794 20
ClaI AT'CG_AT
Cuts at: 1554 2901 1554
Size: 1347 4282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2638 2673 529
Size: 35 2074 35 3485
Fragments arranged by size:
3485 2074 35 35
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DraIII CAC_nnn'GTG
Cuts at: 2923 2923
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2009 2193 3076
Size: 54 1425 50 275 18 179 184 883
Cuts at: 3076 3965 5594 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 883 275 184 179 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 1537
Size: 275 1264 4090
Fragments arranged by size:
4090 1264 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2242 2259 3559 1353
Size: 889 17 1300 3423
Fragments arranged by size:
3423 1300 889 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4216 4590 4736 387
Size: 1064 138 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2380 2380
Size: 5629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2582 3692 4289 5076 5344 1239
Size: 393 950 1110 597 787 268 1524
Fragments arranged by size:
1524 1110 950 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2061 2219 2061
Size: 158 5471
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2288 2288
Size: 5629
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 2752 4776 1678
Size: 1074 2024 2531
Fragments arranged by size:
2531 2024 1074
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2802 2834 4270 4302 4344
Size: 32 135 32 1230 32 1436 32 42
Cuts at: 4344 4376 1373
Size: 32 2626
Fragments arranged by size:
2626 1436 1230 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2150 748
Size: 1402 4227
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KpnI G_GTAC'C
Cuts at: 2844 2954 2844
Size: 110 5519
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 2254 5505 2254
Size: 3251 2378
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2012 4743 4927 1305
Size: 707 2731 184 2007
Fragments arranged by size:
2731 2007 707 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3807
Fragments arranged by size:
3807 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2451 3587 3946 4105 518
Size: 648 1285 1136 359 159 2042
Fragments arranged by size:
2042 1285 1136 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2581 2887 3045 3722 4870 5115 1606
Size: 975 306 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 975 677 306 245 158
NcoI C'CATG_G
Cuts at: 513 1065 2012 513
Size: 552 947 4130
Fragments arranged by size:
4130 947 552
NdeI CA'TA_TG
Cuts at: 387 2707 387
Size: 2320 3309
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2450 2802 2987 4532 1132
Size: 97 1221 352 185 1545 2229
Fragments arranged by size:
2229 1545 1221 352 185 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5629
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2018 2683 1108
Size: 910 665 4054
Fragments arranged by size:
4054 910 665
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2061 2061
Size: 5629
PshAI GACnn'nnGTC
Cuts at: 1613 2833 1613
Size: 1220 4409
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2618 2981 830
Size: 1788 363 3478
Fragments arranged by size:
3478 1788 363
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2887 2887
Size: 5629
SacI G_AGCT'C
Cuts at: 2382 2382
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SapI GCTCTTCn'nnn_
Cuts at: 2259 2259
Size: 5629
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2241 2393 2614 2977 4112 4793 4984
Size: 219 1196 152 221 363 1135 681 191
Cuts at: 4984 826
Size: 1471
Fragments arranged by size:
1471 1196 1135 681 363 221 219 191
152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2182 3733 4634 4896 820
Size: 197 926 239 1551 901 262 1553
Fragments arranged by size:
1553 1551 926 901 262 239 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 2785 1517
Size: 1268 4361
StyI C'CwwG_G
Cuts at: 513 1065 2012 2607 3068 513
Size: 552 947 595 461 3074
Fragments arranged by size:
3074 947 595 552 461
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2729 3656 3673 3826 4011 4456 325
Size: 1255 1149 927 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1149 927 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5587 96
Size: 274 80 33 51 1443 1898 1712 138
Fragments arranged by size:
1898 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5589 5621 5589
Size: 32 5597
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2323
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 2574 3758 2574
Size: 1184 4445
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3437 276
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI