Restriction map for
pFN21AE2711
(Circular) (Six-base) MAPSORT of: pFN21AE2711.seq Check: 922 from: 1 to: 7498
pFN21AE2711 7498 bp
With 182 enzymes: SgfI *
January 19, 2018 17:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5308 278
Size: 53 83 186 936 3772 2468
Fragments arranged by size:
3772 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3360 4841 1937
Size: 1423 1481 4594
Fragments arranged by size:
4594 1481 1423
Acc65I G'GTAC_C
Cuts at: 4541 4819 4541
Size: 278 7220
AclI AA'CG_TT
Cuts at: 1563 5625 5998 1563
Size: 4062 373 3063
Fragments arranged by size:
4062 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 2787 2916 3222 4748 5561 6945 7213
Size: 1010 145 129 306 1526 813 1384 268
Cuts at: 7213 1632
Size: 1917
Fragments arranged by size:
1917 1526 1384 1010 813 306 268 145
129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7301
AflIII A'CryG_T
Cuts at: 1203 6397 7374 1203
Size: 5194 977 1327
Fragments arranged by size:
5194 1327 977
AgeI A'CCGG_T
Cuts at: 3666 6329 3666
Size: 2663 4835
AhdI GACnn_n'nnGTC
Cuts at: 6227 6227
Size: 7498
AleI CACnn'nnGTG
Cuts at: 1166 4127 1166
Size: 2961 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7498
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3039 3071 3039
Size: 32 7466
AlwNI CAG_nnn'CTG
Cuts at: 5241 6813 5241
Size: 1572 5926
ApaLI G'TGCA_C
Cuts at: 5555 6711 5555
Size: 1156 6342
ApoI r'AATT_y
Cuts at: 1493 2068 2726 3011 3347 4807 5033 5185 7294
Size: 575 658 285 336 1460 226 152 2109
Cuts at: 7294 1493
Size: 1697
Fragments arranged by size:
2109 1697 1460 658 575 336 285 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6052 160
Size: 872 5020 1606
Fragments arranged by size:
5020 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7498
AvaI C'yCGr_G
Cuts at: 1870 1943 2100 4199 4581 4823 1870
Size: 73 157 2099 382 242 4545
Fragments arranged by size:
4545 2099 382 242 157 73
BamHI G'GATC_C
Cuts at: 4828 5195 4828
Size: 367 7131
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4025 4541 4819 6274 618
Size: 325 217 627 2238 516 278 1455 1842
Fragments arranged by size:
2238 1842 1455 627 516 325 278 217
BanII G_rGCy'C
Cuts at: 1391 2963 3082 3276 3387 4817 1391
Size: 1572 119 194 111 1430 4072
Fragments arranged by size:
4072 1572 1430 194 119 111
BbeI G_GCGC'C
Cuts at: 4029 4029
Size: 7498
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2909 4084 4484 928
Size: 971 1010 1175 400 3942
Fragments arranged by size:
3942 1175 1010 971 400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 5689 5723 1903
Size: 34 2243 34 1475 34 3678
Fragments arranged by size:
3678 2243 1475 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2525 5390 6600 2525
Size: 2865 1210 3423
Fragments arranged by size:
3423 2865 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7498
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3152 6109 136
Size: 107 122 71 631 605 1480 2957 1525
Fragments arranged by size:
2957 1525 1480 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7493 7493
Size: 7498
BlpI GC'TnA_GC
Cuts at: 3659 4915 3659
Size: 1256 6242
Bme1580I G_kGCm'C
Cuts at: 1790 4273 5559 6715 1790
Size: 2483 1286 1156 2573
Fragments arranged by size:
2573 2483 1286 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7498
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2650 3087 3539
Size: 416 53 83 399 24 1233 437 452
Cuts at: 3539 4787 5229 6187 442
Size: 1248 442 958 1753
Fragments arranged by size:
1753 1248 1233 958 452 442 437 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7498
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 3830 3980 2575
Size: 1255 150 6093
Fragments arranged by size:
6093 1255 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 4547 6158 1239
Size: 993 2315 1611 2579
Fragments arranged by size:
2579 2315 1611 993
Bpu10I CC'TnA_GC
Cuts at: 3948 3948
Size: 7498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2999 3571 5623 6488 6786 2999
Size: 572 2052 865 298 3711
Fragments arranged by size:
3711 2052 865 572 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 4591 6161 882
Size: 617 759 2333 1570 2219
Fragments arranged by size:
2333 2219 1570 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 4182 5289 493
Size: 711 2978 1107 2702
Fragments arranged by size:
2978 2702 1107 711
BsaBI GATnn'nnATC
Cuts at: 2210 7492 2210
Size: 5282 2216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4026 5305 5687 6322
Size: 53 83 186 936 2493 1279 382 635
Cuts at: 6322 275
Size: 1451
Fragments arranged by size:
2493 1451 1279 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3368 3666 5931 6329 6603 6750 7161 3368
Size: 298 2265 398 274 147 411 3705
Fragments arranged by size:
3705 2265 411 398 298 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2152 2182 3311 3341 3819
Size: 30 165 30 749 30 1129 30 478
Cuts at: 3819 3849 1178
Size: 30 4827
Fragments arranged by size:
4827 1129 749 478 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 3366 3569 3572 3969 1186
Size: 861 1319 203 3 397 4715
Fragments arranged by size:
4715 1319 861 397 203 3
BseYI C'CCAG_C
Cuts at: 2761 3107 3427 4255 6701 7197 2761
Size: 346 320 828 2446 496 3062
Fragments arranged by size:
3062 2446 828 496 346 320
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6925
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4054 4948 5709 6737 1540
Size: 275 186 1714 339 894 761 1028 2301
Fragments arranged by size:
2301 1714 1028 894 761 339 275 186
BsiHKAI G_wGCw'C
Cuts at: 3106 3276 5559 5644 6715 3106
Size: 170 2283 85 1071 3889
Fragments arranged by size:
3889 2283 1071 170 85
BsmI GAATG_Cn'
Cuts at: 4191 5009 5102 4191
Size: 818 93 6587
Fragments arranged by size:
6587 818 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3023 4691 3023
Size: 1668 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2963 3082 3106 3276 3387 4273 4817
Size: 399 1173 119 24 170 111 886 544
Cuts at: 4817 5559 5644 6715 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1173 1071 886 742 544 399 170
119 111 85 24
BspEI T'CCGG_A
Cuts at: 3368 7161 3368
Size: 3793 3705
BspHI T'CATG_A
Cuts at: 5387 7117 5387
Size: 1730 5768
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3952 4853 844
Size: 3108 901 3489
Fragments arranged by size:
3489 3108 901
BsrBI CCG'CTC
Cuts at: 1394 1823 2246 3327 5385 1394
Size: 429 423 1081 2058 3507
Fragments arranged by size:
3507 2058 1081 429 423
BsrDI GCAATG_nn'
Cuts at: 65 1248 5993 6167 65
Size: 1183 4745 174 1396
Fragments arranged by size:
4745 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3666 6142 6329 1700
Size: 110 1856 2476 187 2869
Fragments arranged by size:
2869 2476 1856 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7498
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7498
BssSI C'ACGA_G
Cuts at: 3275 3380 5558 6570 3275
Size: 105 2178 1012 4203
Fragments arranged by size:
4203 2178 1012 105
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3476 794
Size: 2682 4816
BstBI TT'CG_AA
Cuts at: 6302 6302
Size: 7498
BstEII G'GTnAC_C
Cuts at: 1184 2329 3538 4322 1184
Size: 1145 1209 784 4360
Fragments arranged by size:
4360 1209 1145 784
BstXI CCAn_nnnn'nTGG
Cuts at: 3181 3301 3181
Size: 120 7378
BstYI r'GATC_y
Cuts at: 1972 4530 4645 4828 5195 5580 5597 7049 7135
Size: 2558 115 183 367 385 17 1452 86
Cuts at: 7135 7147 7493 1972
Size: 12 346 1977
Fragments arranged by size:
2558 1977 1452 385 367 346 183 115
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2582 3180 3331 4565 513
Size: 552 939 578 598 151 1234 3446
Fragments arranged by size:
3446 1234 939 598 578 552 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6759
Fragments arranged by size:
6759 683 56
BtsI GCAGTG_nn'
Cuts at: 2972 3078 4005 5014 5808 5828 2972
Size: 106 927 1009 794 20 4642
Fragments arranged by size:
4642 1009 927 794 106 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7498
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2833 2868 2959 2994 4137 4172 529
Size: 35 2269 35 91 35 1143 35 3855
Fragments arranged by size:
3855 2269 1143 91 35 35 35 35
DraI TTT'AAA
Cuts at: 2725 4802 2725
Size: 2077 5421
DraIII CAC_nnn'GTG
Cuts at: 2531 2531
Size: 7498
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2911 3764 6505 809
Size: 507 1595 853 2741 1802
Fragments arranged by size:
2741 1802 1595 853 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2473 2746
Size: 54 1425 50 275 18 177 466 273
Cuts at: 2746 2787 3132 3712 3726 4701 4945 5834 7463
Size: 41 345 580 14 975 244 889 1629
Cuts at: 7463 8
Size: 43
Fragments arranged by size:
1629 1425 975 889 580 466 345 275
273 244 177 54 50 43 41 18
14
EagI C'GGCC_G
Cuts at: 1537 1812 3712 4945 1537
Size: 275 1900 1233 4090
Fragments arranged by size:
4090 1900 1233 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 2951 5428 1353
Size: 692 906 2477 3423
Fragments arranged by size:
3423 2477 906 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6085 6459 6605 387
Size: 1064 138 4496 374 146 1280
Fragments arranged by size:
4496 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3274 3274
Size: 7498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2642 2787 2916 3222 4547 4748
Size: 393 600 410 145 129 306 1325 201
Cuts at: 4748 5561 6158 6945 7213 1239
Size: 813 597 787 268 1524
Fragments arranged by size:
1524 1325 813 787 600 597 410 393
306 268 201 145 129
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 2093
Size: 7498
EcoO109I rG'GnC_Cy
Cuts at: 3035 3154 3591 3035
Size: 119 437 6942
Fragments arranged by size:
6942 437 119
EcoRI G'AATT_C
Cuts at: 3011 3347 4807 5185 3011
Size: 336 1460 378 5324
Fragments arranged by size:
5324 1460 378 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4729 4729
Size: 7498
FspI TGC'GCA
Cuts at: 3439 6004 3439
Size: 2565 4933
HaeII r_GCGC'y
Cuts at: 1678 4029 6645 1678
Size: 2351 2616 2531
Fragments arranged by size:
2616 2531 2351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 2879 2911 3604
Size: 32 135 32 580 32 695 32 693
Cuts at: 3604 3636 3819 3851 3861 3893 6139 6171 6213
Size: 32 183 32 10 32 2246 32 42
Cuts at: 6213 6245 1373
Size: 32 2626
Fragments arranged by size:
2626 2246 695 693 580 183 135 42
32 32 32 32 32 32 32 32
32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2461 3361 4278 4423 4498 4842
Size: 988 281 523 900 917 145 75 344
Cuts at: 4842 5088 669
Size: 246 3079
Fragments arranged by size:
3079 988 917 900 523 344 281 246
145 75
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7498
HpaI GTT'AAC
Cuts at: 5088 5088
Size: 7498
KasI G'GCGC_C
Cuts at: 4025 4025
Size: 7498
KpnI G_GTAC'C
Cuts at: 4545 4823 4545
Size: 278 7220
MfeI C'AATT_G
Cuts at: 5097 5097
Size: 7498
MluI A'CGCG_T
Cuts at: 7374 7374
Size: 7498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6612 6796 1305
Size: 5307 184 2007
Fragments arranged by size:
5307 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2475 2789 10
Size: 54 1425 343 643 314 4719
Fragments arranged by size:
4719 1425 643 343 314 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 2552 3737 4127 5456 5815 5974
Size: 648 1267 119 1185 390 1329 359 159
Cuts at: 5974 518
Size: 2042
Fragments arranged by size:
2042 1329 1267 1185 648 390 359 159
119
MspA1I CmG'CkG
Cuts at: 1606 2252 2620 2637 2765 4914 5591 6739 6984
Size: 646 368 17 128 2149 677 1148 245
Cuts at: 6984 1606
Size: 2120
Fragments arranged by size:
2149 2120 1148 677 646 368 245 128
17
NarI GG'CG_CC
Cuts at: 4026 4026
Size: 7498
NcoI C'CATG_G
Cuts at: 513 1065 2004 3180 3331 513
Size: 552 939 1176 151 4680
Fragments arranged by size:
4680 1176 939 552 151
NdeI CA'TA_TG
Cuts at: 387 2174 387
Size: 1787 5711
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7498
NotI GC'GGCC_GC
Cuts at: 4945 4945
Size: 7498
NspI r_CATG'y
Cuts at: 1132 1229 4856 6401 1132
Size: 97 3627 1545 2229
Fragments arranged by size:
3627 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7498
PciI A'CATG_T
Cuts at: 6397 6397
Size: 7498
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7498
PfoI T'CCnGG_A
Cuts at: 3863 4472 4533 3863
Size: 609 61 6828
Fragments arranged by size:
6828 609 61
PmeI GTTT'AAAC
Cuts at: 4802 4802
Size: 7498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 5567 5599 7080 7112 3374
Size: 32 2161 32 1481 32 3760
Fragments arranged by size:
3760 2161 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3591 3591
Size: 7498
PshAI GACnn'nnGTC
Cuts at: 1613 4493 1613
Size: 2880 4618
PsiI TTA'TAA
Cuts at: 5068 5068
Size: 7498
PstI C_TGCA'G
Cuts at: 830 2911 4049 4293 4850 830
Size: 2081 1138 244 557 3478
Fragments arranged by size:
3478 2081 1138 557 244
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7498
PvuII CAG'CTG
Cuts at: 2252 2620 2765 2252
Size: 368 145 6985
Fragments arranged by size:
6985 368 145
SacI G_AGCT'C
Cuts at: 3276 3276
Size: 7498
SalI G'TCGA_C
Cuts at: 1936 3359 4840 1936
Size: 1423 1481 4594
Fragments arranged by size:
4594 1481 1423
SapI GCTCTTCn'nnn_
Cuts at: 2951 2951
Size: 7498
SbfI CC_TGCA'GG
Cuts at: 4850 4850
Size: 7498
ScaI AGT'ACT
Cuts at: 5746 7458 5746
Size: 1712 5786
SexAI A'CCwGG_T
Cuts at: 2527 2527
Size: 7498
SfcI C'TryA_G
Cuts at: 826 1045 2149 2566 2907 3889 4045 4225 4289
Size: 219 1104 417 341 982 156 180 64
Cuts at: 4289 4846 5981 6662 6853 826
Size: 557 1135 681 191 1471
Fragments arranged by size:
1471 1135 1104 982 681 557 417 341
219 191 180 156 64
SfoI GGC'GCC
Cuts at: 4027 4027
Size: 7498
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7498
SgrAI Cr'CCGG_yG
Cuts at: 1700 3666 1700
Size: 1966 5532
SmaI CCC'GGG
Cuts at: 1872 4583 4825 1872
Size: 2711 242 4545
Fragments arranged by size:
4545 2711 242
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2978 3586 5602 6503 6765 820
Size: 197 926 1035 608 2016 901 262 1553
Fragments arranged by size:
2016 1553 1035 926 901 608 262 197
SnaBI TAC'GTA
Cuts at: 493 5289 493
Size: 4796 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7498
SphI G_CATG'C
Cuts at: 1132 4856 1132
Size: 3724 3774
SspI AAT'ATT
Cuts at: 5 52 2083 5422 5
Size: 47 2031 3339 2081
Fragments arranged by size:
3339 2081 2031 47
StuI AGG'CCT
Cuts at: 1517 4725 1517
Size: 3208 4290
StyI C'CwwG_G
Cuts at: 513 1065 2004 2094 2844 3180 3331 4125 4336
Size: 552 939 90 750 336 151 794 211
Cuts at: 4336 4431 4937 513
Size: 95 506 3074
Fragments arranged by size:
3074 939 794 750 552 506 336 211
151 95 90
SwaI ATTT'AAAT
Cuts at: 2725 2725
Size: 7498
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3293 3752 5525 5542 5695 5880 6325
Size: 1255 1713 459 1773 17 153 185 445
Cuts at: 6325 325
Size: 1498
Fragments arranged by size:
1773 1713 1498 1255 459 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5744 7456 96
Size: 274 80 33 51 1443 3767 1712 138
Fragments arranged by size:
3767 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3781 3813 7458 7490 3781
Size: 32 3645 32 3789
Fragments arranged by size:
3789 3645 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3982 1316
Size: 2666 4832
XbaI T'CTAG_A
Cuts at: 4834 4834
Size: 7498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 3115 3407 4433 4552 2341
Size: 774 292 1026 119 5287
Fragments arranged by size:
5287 1026 774 292 119
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7498
XmaI C'CCGG_G
Cuts at: 1870 4581 4823 1870
Size: 2711 242 4545
Fragments arranged by size:
4545 2711 242
XmnI GAAnn'nnTTC
Cuts at: 4712 5627 4712
Size: 915 6583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5306 276
Size: 53 83 186 936 3772 2468
Fragments arranged by size:
3772 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SrfI