Restriction map for
pFN21AE2704
(Circular) (Six-base) MAPSORT of: pFN21AE2704.seq Check: 1588 from: 1 to: 8752
pFN21AE2704 8752 bp
With 182 enzymes: SgfI *
September 21, 2017 12:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6562 278
Size: 53 83 186 936 5026 2468
Fragments arranged by size:
5026 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2351 2959 3386 6095 1937
Size: 414 608 427 2709 4594
Fragments arranged by size:
4594 2709 608 427 414
Acc65I G'GTAC_C
Cuts at: 6073 6073
Size: 8752
AclI AA'CG_TT
Cuts at: 1563 2382 6879 7252 1563
Size: 819 4497 373 3063
Fragments arranged by size:
4497 3063 819 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3246 3504 4466 4914 5048 5075 5217 5399
Size: 1614 258 962 448 134 27 142 182
Cuts at: 5399 5694 5769 5994 6815 8199 8467 1632
Size: 295 75 225 821 1384 268 1917
Fragments arranged by size:
1917 1614 1384 962 821 448 295 268
258 225 182 142 134 75 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8555
AflIII A'CryG_T
Cuts at: 1203 7651 8628 1203
Size: 6448 977 1327
Fragments arranged by size:
6448 1327 977
AgeI A'CCGG_T
Cuts at: 7583 7583
Size: 8752
AhdI GACnn_n'nnGTC
Cuts at: 7481 7481
Size: 8752
AleI CACnn'nnGTG
Cuts at: 1166 5741 1166
Size: 4575 4177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8752
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4827 4859 5675 5707 4827
Size: 32 816 32 7872
Fragments arranged by size:
7872 816 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2166 5972 6495 8067 2166
Size: 3806 523 1572 2851
Fragments arranged by size:
3806 2851 1572 523
ApaLI G'TGCA_C
Cuts at: 6809 7965 6809
Size: 1156 7596
ApoI r'AATT_y
Cuts at: 1493 2097 2203 2245 2702 2846 3525 3582 4235
Size: 604 106 42 457 144 679 57 653
Cuts at: 4235 4422 4518 4813 6061 6287 6439 8548 1493
Size: 187 96 295 1248 226 152 2109 1697
Fragments arranged by size:
2109 1697 1248 679 653 604 457 295
226 187 152 144 106 96 57 42
AseI AT'TA_AT
Cuts at: 160 1032 2307 7306 160
Size: 872 1275 4999 1606
Fragments arranged by size:
4999 1606 1275 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8752
AvaI C'yCGr_G
Cuts at: 1870 1943 2767 4660 4947 5767 6077 1870
Size: 73 824 1893 287 820 310 4545
Fragments arranged by size:
4545 1893 824 820 310 287 73
BamHI G'GATC_C
Cuts at: 5460 6082 6449 5460
Size: 622 367 7763
Fragments arranged by size:
7763 622 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2721 6073 7528 618
Size: 325 217 627 934 3352 1455 1842
Fragments arranged by size:
3352 1842 1455 934 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2085 2092 2768 4884 6071 1391
Size: 694 7 676 2116 1187 4072
Fragments arranged by size:
4072 2116 1187 694 676 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2637 3627 3840 4020 4920 5495 5732
Size: 971 738 990 213 180 900 575 237
Cuts at: 5732 928
Size: 3948
Fragments arranged by size:
3948 990 971 900 738 575 237 213
180
BbvCI CC'TCA_GC
Cuts at: 5097 5103 5646 5097
Size: 6 543 8203
Fragments arranged by size:
8203 543 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6943 6977 1903
Size: 34 5006 34 3678
Fragments arranged by size:
5006 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6644 7854 6644
Size: 1210 7542
BclI T'GATC_A
Cuts at: 1548 5883 1548
Size: 4335 4417
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5581 7363 136
Size: 107 122 71 631 605 3909 1782 1525
Fragments arranged by size:
3909 1782 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2443 3265 3788 5267 8747 2443
Size: 822 523 1479 3480 2448
Fragments arranged by size:
3480 2448 1479 822 523
BlpI GC'TnA_GC
Cuts at: 6169 6169
Size: 8752
Bme1580I G_kGCm'C
Cuts at: 1790 4660 5703 6813 7969 1790
Size: 2870 1043 1110 1156 2573
Fragments arranged by size:
2870 2573 1156 1110 1043
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6483 7441 442
Size: 416 53 83 399 24 5066 958 1753
Fragments arranged by size:
5066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5592 5592
Size: 8752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2460 2620 3452 4083 4930 5677 7412 1239
Size: 1221 160 832 631 847 747 1735 2579
Fragments arranged by size:
2579 1735 1221 847 832 747 631 160
Bpu10I CC'TnA_GC
Cuts at: 4329 4847 5097 5103 5646 4329
Size: 518 250 6 543 7435
Fragments arranged by size:
7435 543 518 250 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3335 4081 4320 6877 7742 8040 3335
Size: 746 239 2557 865 298 4047
Fragments arranged by size:
4047 2557 865 746 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7415 882
Size: 617 5916 2219
Fragments arranged by size:
5916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6543 493
Size: 711 5339 2702
Fragments arranged by size:
5339 2702 711
BsaBI GATnn'nnATC
Cuts at: 6028 8746 6028
Size: 2718 6034
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 4145 6559 6941
Size: 53 83 186 936 792 1820 2414 382
Cuts at: 6941 7576 275
Size: 635 1451
Fragments arranged by size:
2414 1820 1451 936 792 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3702 7185 7583 7857 8004 8415 3702
Size: 3483 398 274 147 411 4039
Fragments arranged by size:
4039 3483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5531 5561 5675 5705 5961
Size: 30 165 30 4128 30 114 30 256
Cuts at: 5961 5991 1178
Size: 30 3939
Fragments arranged by size:
4128 3939 256 165 114 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2703 2779 3182 4700 4703 4976 4981 5120
Size: 1517 76 403 1518 3 273 5 139
Cuts at: 5120 5141 5371 5680 1186
Size: 21 230 309 4258
Fragments arranged by size:
4258 1518 1517 403 309 273 230 139
76 21 5 3
BseYI C'CCAG_C
Cuts at: 2424 2530 3622 4228 4668 7955 8451 2424
Size: 106 1092 606 440 3287 496 2725
Fragments arranged by size:
3287 2725 1092 606 496 440 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3858 1163
Size: 573 2122 6057
Fragments arranged by size:
6057 2122 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6202 6963 7991 1540
Size: 275 186 4201 761 1028 2301
Fragments arranged by size:
4201 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2092 2122 3656 6813 6898 7969 2085
Size: 7 30 1534 3157 85 1071 2868
Fragments arranged by size:
3157 2868 1534 1071 85 30 7
BsmI GAATG_Cn'
Cuts at: 6263 6356 6263
Size: 93 8659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2092 2122 2768 3656 4660 4884
Size: 399 295 7 30 646 888 1004 224
Cuts at: 4884 5703 6071 6813 6898 7969 1391
Size: 819 368 742 85 1071 2174
Fragments arranged by size:
2174 1071 1004 888 819 742 646 399
368 295 224 85 30 7
BspEI T'CCGG_A
Cuts at: 8415 8415
Size: 8752
BspHI T'CATG_A
Cuts at: 4522 4993 5514 6641 8371 4522
Size: 471 521 1127 1730 4903
Fragments arranged by size:
4903 1730 1127 521 471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4211 6107 844
Size: 3367 1896 3489
Fragments arranged by size:
3489 3367 1896
BsrBI CCG'CTC
Cuts at: 1394 1823 4693 5155 6639 1394
Size: 429 2870 462 1484 3507
Fragments arranged by size:
3507 2870 1484 462 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4540 7247 7421 65
Size: 1183 3292 2707 174 1396
Fragments arranged by size:
3292 2707 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7396 7583 1700
Size: 110 5586 187 2869
Fragments arranged by size:
5586 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8752
BssSI C'ACGA_G
Cuts at: 3672 6812 7824 3672
Size: 3140 1012 4600
Fragments arranged by size:
4600 3140 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6012 794
Size: 5218 3534
BstBI TT'CG_AA
Cuts at: 7556 7556
Size: 8752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8752
BstXI CCAn_nnnn'nTGG
Cuts at: 4148 4825 5743 4148
Size: 677 918 7157
Fragments arranged by size:
7157 918 677
BstYI r'GATC_y
Cuts at: 1972 2443 3265 3788 4100 4197 5267 5360 5460
Size: 471 822 523 312 97 1070 93 100
Cuts at: 5460 6082 6449 6834 6851 8303 8389 8401 8747
Size: 622 367 385 17 1452 86 12 346
Cuts at: 8747 1972
Size: 1977
Fragments arranged by size:
1977 1452 1070 822 622 523 471 385
367 346 312 100 97 93 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 5965 5965
Size: 8752
BtgI C'CryG_G
Cuts at: 513 1065 4323 6048 513
Size: 552 3258 1725 3217
Fragments arranged by size:
3258 3217 1725 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8013
Fragments arranged by size:
8013 683 56
BtsI GCAGTG_nn'
Cuts at: 5479 6268 7062 7082 5479
Size: 789 794 20 7149
Fragments arranged by size:
7149 794 789 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3840 3875 529
Size: 35 3276 35 5406
Fragments arranged by size:
5406 3276 35 35
DraI TTT'AAA
Cuts at: 6056 6056
Size: 8752
DraIII CAC_nnn'GTG
Cuts at: 4354 4354
Size: 8752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7759 809
Size: 507 6443 1802
Fragments arranged by size:
6443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5745 6038 6199
Size: 54 1425 50 275 18 3915 293 161
Cuts at: 6199 7088 8717 8
Size: 889 1629 43
Fragments arranged by size:
3915 1629 1425 889 293 275 161 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6199 1537
Size: 275 4387 4090
Fragments arranged by size:
4387 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2478 3279 3492 4148 4770 4953 5064
Size: 719 406 801 213 656 622 183 111
Cuts at: 5064 5353 5423 5515 5965 6682 1353
Size: 289 70 92 450 717 3423
Fragments arranged by size:
3423 801 719 717 656 622 450 406
289 213 183 111 92 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7339 7713 7859 387
Size: 1064 138 5750 374 146 1280
Fragments arranged by size:
5750 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2083 2090 2083
Size: 7 8745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2460 2620 3246 3452 3504 4083 4466
Size: 393 828 160 626 206 52 579 383
Cuts at: 4466 4914 4930 5048 5075 5217 5399 5677 5694
Size: 448 16 118 27 142 182 278 17
Cuts at: 5694 5769 5994 6815 7412 8199 8467 1239
Size: 75 225 821 597 787 268 1524
Fragments arranged by size:
1524 828 821 787 626 597 579 448
393 383 278 268 225 206 182 160
142 118 75 52 27 17 16
EcoNI CCTnn'n_nnAGG
Cuts at: 4736 4736
Size: 8752
EcoRI G'AATT_C
Cuts at: 4518 4813 6061 6439 4518
Size: 295 1248 378 6831
Fragments arranged by size:
6831 1248 378 295
EcoRV GAT'ATC
Cuts at: 2797 2797
Size: 8752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2444 3276 4554 2444
Size: 832 1278 6642
Fragments arranged by size:
6642 1278 832
FspI TGC'GCA
Cuts at: 7258 7258
Size: 8752
HaeII r_GCGC'y
Cuts at: 1678 4372 5140 7899 1678
Size: 2694 768 2759 2531
Fragments arranged by size:
2759 2694 2531 768
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2104 2136 2457 2489 2870
Size: 32 135 32 532 32 321 32 381
Cuts at: 2870 2902 3353 3385 4037 4069 5276 5308 5961
Size: 32 451 32 652 32 1207 32 653
Cuts at: 5961 5993 7393 7425 7467 7499 1373
Size: 32 1400 32 42 32 2626
Fragments arranged by size:
2626 1400 1207 653 652 532 451 381
321 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3726 5485 6096 6342 669
Size: 988 281 1788 1759 611 246 3079
Fragments arranged by size:
3079 1788 1759 988 611 281 246
HindIII A'AGCT_T
Cuts at: 748 2312 2394 2749 3907 748
Size: 1564 82 355 1158 5593
Fragments arranged by size:
5593 1564 1158 355 82
HpaI GTT'AAC
Cuts at: 3726 6342 3726
Size: 2616 6136
KpnI G_GTAC'C
Cuts at: 6077 6077
Size: 8752
MfeI C'AATT_G
Cuts at: 6351 6351
Size: 8752
MluI A'CGCG_T
Cuts at: 8628 8628
Size: 8752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2027 4670 5488 7866 8050 1305
Size: 722 2643 818 2378 184 2007
Fragments arranged by size:
2643 2378 2007 818 722 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5747 10
Size: 54 1425 343 3915 3015
Fragments arranged by size:
3915 3015 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 3659 5741 6710 7069 7228 518
Size: 648 969 1524 2082 969 359 159 2042
Fragments arranged by size:
2082 2042 1524 969 969 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3157 5457 5650 5714 5809 6168 6845 7993
Size: 1551 2300 193 64 95 359 677 1148
Cuts at: 7993 8238 1606
Size: 245 2120
Fragments arranged by size:
2300 2120 1551 1148 677 359 245 193
95 64
NcoI C'CATG_G
Cuts at: 513 1065 4323 6048 513
Size: 552 3258 1725 3217
Fragments arranged by size:
3258 3217 1725 552
NdeI CA'TA_TG
Cuts at: 387 5840 387
Size: 5453 3299
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8752
NotI GC'GGCC_GC
Cuts at: 6199 6199
Size: 8752
NspI r_CATG'y
Cuts at: 1132 1229 4864 5680 6110 7655 1132
Size: 97 3635 816 430 1545 2229
Fragments arranged by size:
3635 2229 1545 816 430 97
PasI CC'CwG_GG
Cuts at: 1348 5896 1348
Size: 4548 4204
PciI A'CATG_T
Cuts at: 7651 7651
Size: 8752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5565 1108
Size: 4457 4295
PfoI T'CCnGG_A
Cuts at: 2437 2437
Size: 8752
PmeI GTTT'AAAC
Cuts at: 6056 6056
Size: 8752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5330 5362 5675 5707 6821 6853 8334 8366 5330
Size: 32 313 32 1114 32 1481 32 5716
Fragments arranged by size:
5716 1481 1114 313 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 5631 1613
Size: 4018 4734
PsiI TTA'TAA
Cuts at: 2871 6322 2871
Size: 3451 5301
PspXI vC'TCGA_Gb
Cuts at: 2767 2767
Size: 8752
PstI C_TGCA'G
Cuts at: 830 2171 3273 3360 4740 6104 830
Size: 1341 1102 87 1380 1364 3478
Fragments arranged by size:
3478 1380 1364 1341 1102 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8752
PvuII CAG'CTG
Cuts at: 3157 5457 5650 5714 5809 3157
Size: 2300 193 64 95 6100
Fragments arranged by size:
6100 2300 193 95 64
SacI G_AGCT'C
Cuts at: 2085 2092 2085
Size: 7 8745
SalI G'TCGA_C
Cuts at: 1936 6094 1936
Size: 4158 4594
SapI GCTCTTCn'nnn_
Cuts at: 5064 5965 5064
Size: 901 7851
SbfI CC_TGCA'GG
Cuts at: 6104 6104
Size: 8752
ScaI AGT'ACT
Cuts at: 4823 7000 8712 4823
Size: 2177 1712 4863
Fragments arranged by size:
4863 2177 1712
SfcI C'TryA_G
Cuts at: 826 1045 2167 2447 3269 3356 3387 4736 6100
Size: 219 1122 280 822 87 31 1349 1364
Cuts at: 6100 7235 7916 8107 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1364 1349 1135 1122 822 681 280
219 191 87 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8752
SmaI CCC'GGG
Cuts at: 1872 6079 1872
Size: 4207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2767 3314 4096 4335 6856 7757
Size: 197 926 824 547 782 239 2521 901
Cuts at: 7757 8019 820
Size: 262 1553
Fragments arranged by size:
2521 1553 926 901 824 782 547 262
239 197
SnaBI TAC'GTA
Cuts at: 493 6543 493
Size: 6050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8752
SphI G_CATG'C
Cuts at: 1132 6110 1132
Size: 4978 3774
SspI AAT'ATT
Cuts at: 5 52 4495 5321 6676 5
Size: 47 4443 826 1355 2081
Fragments arranged by size:
4443 2081 1355 826 47
StuI AGG'CCT
Cuts at: 1517 5191 1517
Size: 3674 5078
StyI C'CwwG_G
Cuts at: 513 1065 2717 4323 5002 5579 6048 6191 513
Size: 552 1652 1606 679 577 469 143 3074
Fragments arranged by size:
3074 1652 1606 679 577 552 469 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4660 6779 6796 6949 7134 7579 325
Size: 1255 3080 2119 17 153 185 445 1498
Fragments arranged by size:
3080 2119 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 4821 5181
Size: 274 80 33 51 1443 546 2298 360
Cuts at: 5181 6000 6998 8710 96
Size: 819 998 1712 138
Fragments arranged by size:
2298 1712 1443 998 819 546 360 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4338 4370 8712 8744 4338
Size: 32 4342 32 4346
Fragments arranged by size:
4346 4342 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2162 3463 1316
Size: 846 1301 6605
Fragments arranged by size:
6605 1301 846
XbaI T'CTAG_A
Cuts at: 2275 6088 2275
Size: 3813 4939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5740 5740
Size: 8752
XhoI C'TCGA_G
Cuts at: 1943 2767 1943
Size: 824 7928
XmaI C'CCGG_G
Cuts at: 1870 6077 1870
Size: 4207 4545
XmnI GAAnn'nnTTC
Cuts at: 2899 3067 3138 3412 4479 4989 5776 6881 2899
Size: 168 71 274 1067 510 787 1105 4770
Fragments arranged by size:
4770 1105 1067 787 510 274 168 71
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6560 276
Size: 53 83 186 936 5026 2468
Fragments arranged by size:
5026 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI