Restriction map for
pFN21AE2703
(Circular) (Six-base) MAPSORT of: pFN21AE2703.seq Check: 8284 from: 1 to: 7300
pFN21AE2703 7300 bp
With 182 enzymes: SgfI *
September 21, 2017 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3735 3735
Size: 7300
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5110 278
Size: 53 83 186 936 3574 2468
Fragments arranged by size:
3574 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2313 4643 1937
Size: 376 2330 4594
Fragments arranged by size:
4594 2330 376
Acc65I G'GTAC_C
Cuts at: 4621 4621
Size: 7300
AclI AA'CG_TT
Cuts at: 1563 3036 3045 5427 5800 1563
Size: 1473 9 2382 373 3063
Fragments arranged by size:
3063 2382 1473 373 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3827 3842 5363 6747 7015 1632
Size: 2195 15 1521 1384 268 1917
Fragments arranged by size:
2195 1917 1521 1384 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7103
AflIII A'CryG_T
Cuts at: 1203 4482 4597 6199 7176 1203
Size: 3279 115 1602 977 1327
Fragments arranged by size:
3279 1602 1327 977 115
AgeI A'CCGG_T
Cuts at: 6131 6131
Size: 7300
AhdI GACnn_n'nnGTC
Cuts at: 6029 6029
Size: 7300
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7300
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2666 2698 2666
Size: 32 7268
AlwNI CAG_nnn'CTG
Cuts at: 2288 3690 5043 6615 2288
Size: 1402 1353 1572 2973
Fragments arranged by size:
2973 1572 1402 1353
ApaI G_GGCC'C
Cuts at: 2460 2460
Size: 7300
ApaLI G'TGCA_C
Cuts at: 5357 6513 5357
Size: 1156 6144
ApoI r'AATT_y
Cuts at: 1493 2237 2724 3163 3271 3997 4378 4609 4835
Size: 744 487 439 108 726 381 231 226
Cuts at: 4835 4987 7096 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 744 726 487 439 381 231
226 152 108
AseI AT'TA_AT
Cuts at: 160 1032 3765 5854 160
Size: 872 2733 2089 1606
Fragments arranged by size:
2733 2089 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7300
AvaI C'yCGr_G
Cuts at: 1870 1943 3417 4625 1870
Size: 73 1474 1208 4545
Fragments arranged by size:
4545 1474 1208 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4323 4356 4323
Size: 33 7267
BamHI G'GATC_C
Cuts at: 4630 4997 4630
Size: 367 6933
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4621 6076 618
Size: 325 217 627 2834 1455 1842
Fragments arranged by size:
2834 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2460 4619 1391
Size: 1069 2159 4072
Fragments arranged by size:
4072 2159 1069
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3194 3439 3618 928
Size: 971 1295 245 179 4610
Fragments arranged by size:
4610 1295 971 245 179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5491 5525 1903
Size: 34 3554 34 3678
Fragments arranged by size:
3678 3554 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3583 5192 6402 3583
Size: 1609 1210 4481
Fragments arranged by size:
4481 1609 1210
BclI T'GATC_A
Cuts at: 1548 3498 1548
Size: 1950 5350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5911 136
Size: 107 122 71 631 605 4239 1525
Fragments arranged by size:
4239 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2689 7295 2689
Size: 4606 2694
BlpI GC'TnA_GC
Cuts at: 2864 4717 2864
Size: 1853 5447
Bme1580I G_kGCm'C
Cuts at: 1790 2460 5361 6517 1790
Size: 670 2901 1156 2573
Fragments arranged by size:
2901 2573 1156 670
BmgBI CAC'GTC
Cuts at: 1318 4483 1318
Size: 3165 4135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2650 3625 5031
Size: 416 53 83 399 24 1233 975 1406
Cuts at: 5031 5989 442
Size: 958 1753
Fragments arranged by size:
1753 1406 1233 975 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2613 5960 1239
Size: 1374 3347 2579
Fragments arranged by size:
3347 2579 1374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5425 6290 6588 5425
Size: 865 298 6137
Fragments arranged by size:
6137 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 2734 2792 5963 882
Size: 617 624 611 58 3171 2219
Fragments arranged by size:
3171 2219 624 617 611 58
BsaAI yAC'GTr
Cuts at: 493 1204 5091 493
Size: 711 3887 2702
Fragments arranged by size:
3887 2702 711
BsaBI GATnn'nnATC
Cuts at: 7294 7294
Size: 7300
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3444 5107 5489 6124
Size: 53 83 186 936 1911 1663 382 635
Cuts at: 6124 275
Size: 1451
Fragments arranged by size:
1911 1663 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3437 5733 6131 6405 6552 6963 3437
Size: 2296 398 274 147 411 3774
Fragments arranged by size:
3774 2296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2071 2101 4469 4499 1178
Size: 30 165 30 668 30 2368 30 3979
Fragments arranged by size:
3979 2368 668 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7300
BseYI C'CCAG_C
Cuts at: 2035 3310 6503 6999 2035
Size: 1275 3193 496 2336
Fragments arranged by size:
3193 2336 1275 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3858 1163
Size: 573 2122 4605
Fragments arranged by size:
4605 2122 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4750 5511 6539 1540
Size: 275 186 2749 761 1028 2301
Fragments arranged by size:
2749 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4085 5361 5446 6517 4085
Size: 1276 85 1071 4868
Fragments arranged by size:
4868 1276 1071 85
BsmI GAATG_Cn'
Cuts at: 2163 3227 4811 4904 2163
Size: 1064 1584 93 4559
Fragments arranged by size:
4559 1584 1064 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2460 4085 4619 5361 5446 6517 1391
Size: 399 670 1625 534 742 85 1071 2174
Fragments arranged by size:
2174 1625 1071 742 670 534 399 85
BspEI T'CCGG_A
Cuts at: 6963 6963
Size: 7300
BspHI T'CATG_A
Cuts at: 5189 6919 5189
Size: 1730 5570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 3134 3735 4655 844
Size: 2113 177 601 920 3489
Fragments arranged by size:
3489 2113 920 601 177
BsrBI CCG'CTC
Cuts at: 1394 1823 4325 5187 1394
Size: 429 2502 862 3507
Fragments arranged by size:
3507 2502 862 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5795 5969 65
Size: 1183 4547 174 1396
Fragments arranged by size:
4547 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5944 6131 1700
Size: 110 4134 187 2869
Fragments arranged by size:
4134 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7300
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7300
BssSI C'ACGA_G
Cuts at: 3562 5360 6372 3562
Size: 1798 1012 4490
Fragments arranged by size:
4490 1798 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7300
BstBI TT'CG_AA
Cuts at: 6104 6104
Size: 7300
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7300
BstXI CCAn_nnnn'nTGG
Cuts at: 2150 2150
Size: 7300
BstYI r'GATC_y
Cuts at: 1972 2689 4630 4997 5382 5399 6851 6937 6949
Size: 717 1941 367 385 17 1452 86 12
Cuts at: 6949 7295 1972
Size: 346 1977
Fragments arranged by size:
1977 1941 1452 717 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2091 2465 2091
Size: 374 6926
BtgI C'CryG_G
Cuts at: 513 1065 2004 2778 3015 513
Size: 552 939 774 237 4798
Fragments arranged by size:
4798 939 774 552 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6561
Fragments arranged by size:
6561 683 56
BtsI GCAGTG_nn'
Cuts at: 3796 4274 4816 5610 5630 3796
Size: 478 542 794 20 5466
Fragments arranged by size:
5466 794 542 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7300
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2404 2439 529
Size: 35 1840 35 5390
Fragments arranged by size:
5390 1840 35 35
DraI TTT'AAA
Cuts at: 4604 4604
Size: 7300
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6307 809
Size: 507 4991 1802
Fragments arranged by size:
4991 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4317 4747 5636
Size: 54 1425 50 275 18 2487 430 889
Cuts at: 5636 7265 8
Size: 1629 43
Fragments arranged by size:
2487 1629 1425 889 430 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4747 1537
Size: 275 2935 4090
Fragments arranged by size:
4090 2935 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2831 3282 3726 5230 1353
Size: 1478 451 444 1504 3423
Fragments arranged by size:
3423 1504 1478 451 444
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2736 5887 6261 6407 387
Size: 1064 138 1147 3151 374 146 1280
Fragments arranged by size:
3151 1280 1147 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2613 3827 3842 5363 5960 6747 7015
Size: 393 981 1214 15 1521 597 787 268
Cuts at: 7015 1239
Size: 1524
Fragments arranged by size:
1524 1521 1214 981 787 597 393 268
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2464 2464
Size: 7300
EcoO109I rG'GnC_Cy
Cuts at: 2457 2457
Size: 7300
EcoRI G'AATT_C
Cuts at: 2724 3163 4609 4987 2724
Size: 439 1446 378 5037
Fragments arranged by size:
5037 1446 439 378
EcoRV GAT'ATC
Cuts at: 3181 3181
Size: 7300
FspI TGC'GCA
Cuts at: 5806 5806
Size: 7300
HaeII r_GCGC'y
Cuts at: 1678 6447 1678
Size: 4769 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2733 2759 2765 2791 3206
Size: 32 135 32 1161 26 6 26 415
Cuts at: 3206 3238 3411 3443 5941 5973 6015 6047 1373
Size: 32 173 32 2498 32 42 32 2626
Fragments arranged by size:
2626 2498 1161 415 173 135 42 32
32 32 32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4644 4890 669
Size: 988 281 2706 246 3079
Fragments arranged by size:
3079 2706 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7300
HpaI GTT'AAC
Cuts at: 4890 4890
Size: 7300
KpnI G_GTAC'C
Cuts at: 4625 4625
Size: 7300
MfeI C'AATT_G
Cuts at: 4899 4899
Size: 7300
MluI A'CGCG_T
Cuts at: 7176 7176
Size: 7300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2555 2793 3100 3439 3445 3456 4113 6414
Size: 1250 238 307 339 6 11 657 2301
Cuts at: 6414 6598 1305
Size: 184 2007
Fragments arranged by size:
2301 2007 1250 657 339 307 238 184
11 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4319 10
Size: 54 1425 343 2487 2991
Fragments arranged by size:
2991 2487 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3304 4557 5258 5617 5776 518
Size: 648 2138 1253 701 359 159 2042
Fragments arranged by size:
2138 2042 1253 701 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2980 4716 5393 6541 6786 1606
Size: 1374 1736 677 1148 245 2120
Fragments arranged by size:
2120 1736 1374 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2778 513
Size: 552 939 774 5035
Fragments arranged by size:
5035 939 774 552
NdeI CA'TA_TG
Cuts at: 387 2254 3958 387
Size: 1867 1704 3729
Fragments arranged by size:
3729 1867 1704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7300
NotI GC'GGCC_GC
Cuts at: 4747 4747
Size: 7300
NspI r_CATG'y
Cuts at: 1132 1229 3404 4601 4658 6203 1132
Size: 97 2175 1197 57 1545 2229
Fragments arranged by size:
2229 2175 1545 1197 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7300
PciI A'CATG_T
Cuts at: 4597 6199 4597
Size: 1602 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7300
PmeI GTTT'AAAC
Cuts at: 4604 4604
Size: 7300
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5369 5401 6882 6914 5369
Size: 32 1481 32 5755
Fragments arranged by size:
5755 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7300
PsiI TTA'TAA
Cuts at: 4870 4870
Size: 7300
PspOMI G'GGCC_C
Cuts at: 2456 2456
Size: 7300
PstI C_TGCA'G
Cuts at: 830 3732 4269 4652 830
Size: 2902 537 383 3478
Fragments arranged by size:
3478 2902 537 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7300
PvuII CAG'CTG
Cuts at: 2980 2980
Size: 7300
SalI G'TCGA_C
Cuts at: 1936 4642 1936
Size: 2706 4594
SapI GCTCTTCn'nnn_
Cuts at: 2831 2831
Size: 7300
SbfI CC_TGCA'GG
Cuts at: 4652 4652
Size: 7300
ScaI AGT'ACT
Cuts at: 4457 5548 7260 4457
Size: 1091 1712 4497
Fragments arranged by size:
4497 1712 1091
SexAI A'CCwGG_T
Cuts at: 2547 2547
Size: 7300
SfcI C'TryA_G
Cuts at: 826 1045 3728 4065 4265 4648 5783 6464 6655
Size: 219 2683 337 200 383 1135 681 191
Cuts at: 6655 826
Size: 1471
Fragments arranged by size:
2683 1471 1135 681 383 337 219 200
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7300
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7300
SmaI CCC'GGG
Cuts at: 1872 4627 1872
Size: 2755 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5404 6305 6567 820
Size: 197 926 3461 901 262 1553
Fragments arranged by size:
3461 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5091 493
Size: 4598 2702
SpeI A'CTAG_T
Cuts at: 152 2176 152
Size: 2024 5276
SphI G_CATG'C
Cuts at: 1132 3404 4658 1132
Size: 2272 1254 3774
Fragments arranged by size:
3774 2272 1254
SspI AAT'ATT
Cuts at: 5 52 5224 5
Size: 47 5172 2081
Fragments arranged by size:
5172 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7300
StyI C'CwwG_G
Cuts at: 513 1065 2004 2670 2778 4739 513
Size: 552 939 666 108 1961 3074
Fragments arranged by size:
3074 1961 939 666 552 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2748 3302 3824 5327 5344 5497 5682
Size: 1255 1168 554 522 1503 17 153 185
Cuts at: 5682 6127 325
Size: 445 1498
Fragments arranged by size:
1503 1498 1255 1168 554 522 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2658 2855 3115
Size: 274 80 33 51 1443 681 197 260
Cuts at: 3115 4455 5546 7258 96
Size: 1340 1091 1712 138
Fragments arranged by size:
1712 1443 1340 1091 681 274 260 197
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2071 2103 7260 7292 2071
Size: 32 5157 32 2079
Fragments arranged by size:
5157 2079 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7300
XbaI T'CTAG_A
Cuts at: 3964 4327 4636 3964
Size: 363 309 6628
Fragments arranged by size:
6628 363 309
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2809 4516 2809
Size: 1707 5593
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7300
XmaI C'CCGG_G
Cuts at: 1870 4625 1870
Size: 2755 4545
XmnI GAAnn'nnTTC
Cuts at: 3737 4471 5429 3737
Size: 734 958 5608
Fragments arranged by size:
5608 958 734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5108 276
Size: 53 83 186 936 3574 2468
Fragments arranged by size:
3574 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI