Restriction map for
pFN21AE2701
(Circular) (Six-base) MAPSORT of: pFN21AE2701.seq Check: 8477 from: 1 to: 18346
pFN21AE2701 18346 bp
With 182 enzymes: SgfI *
August 18, 2017 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4444 6499 7759 8323 8542 8818 9850 12661 15320
Size: 2055 1260 564 219 276 1032 2811 2659
Cuts at: 15320 4444
Size: 7470
Fragments arranged by size:
7470 2811 2659 2055 1260 1032 564 276
219
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10716 14396 16156 278
Size: 53 83 186 936 9180 3680 1760 2468
Fragments arranged by size:
9180 3680 2468 1760 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 10689 13089 15689 1937
Size: 8752 2400 2600 4594
Fragments arranged by size:
8752 4594 2600 2400
Acc65I G'GTAC_C
Cuts at: 2526 15667 2526
Size: 13141 5205
AclI AA'CG_TT
Cuts at: 1563 16473 16846 1563
Size: 14910 373 3063
Fragments arranged by size:
14910 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2864 2990 3170 3500 4025 4178 4406 4775
Size: 1232 126 180 330 525 153 228 369
Cuts at: 4775 5420 7814 8375 8660 10133 11156 11384 11902
Size: 645 2394 561 285 1473 1023 228 518
Cuts at: 11902 12469 13973 14129 14387 15146 16409 17793 18061
Size: 567 1504 156 258 759 1263 1384 268
Cuts at: 18061 1632
Size: 1917
Fragments arranged by size:
2394 1917 1504 1473 1384 1263 1232 1023
759 645 567 561 525 518 369 330
285 268 258 228 228 180 156 153
126
AfeI AGC'GCT
Cuts at: 4955 5531 5564 6497 6785 6902 8795 9142 9218
Size: 576 33 933 288 117 1893 347 76
Cuts at: 9218 9524 10613 11018 14990 15161 4955
Size: 306 1089 405 3972 171 8140
Fragments arranged by size:
8140 3972 1893 1089 933 576 405 347
306 288 171 117 76 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 18149
AflIII A'CryG_T
Cuts at: 1203 6802 17245 18222 1203
Size: 5599 10443 977 1327
Fragments arranged by size:
10443 5599 1327 977
AgeI A'CCGG_T
Cuts at: 13922 14981 17177 13922
Size: 1059 2196 15091
Fragments arranged by size:
15091 2196 1059
AhdI GACnn_n'nnGTC
Cuts at: 2749 13513 17075 2749
Size: 10764 3562 4020
Fragments arranged by size:
10764 4020 3562
AleI CACnn'nnGTG
Cuts at: 1166 2193 4476 5056 10037 10889 12671 1166
Size: 1027 2283 580 4981 852 1782 6841
Fragments arranged by size:
6841 4981 2283 1782 1027 852 580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6362 6738 9882 10433 10961 12410 14144 15401
Size: 5178 376 3144 551 528 1449 1734 1257
Cuts at: 15401 1184
Size: 4129
Fragments arranged by size:
5178 4129 3144 1734 1449 1257 551 528
376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2906 2907 2938 2939 2906
Size: 1 31 1 18313
Fragments arranged by size:
18313 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2536 2968 3586 4225 4639 4906 4909 4918 5764
Size: 432 618 639 414 267 3 9 846
Cuts at: 5764 6277 6436 6616 7129 7198 7261 7369 8209
Size: 513 159 180 513 69 63 108 840
Cuts at: 8209 8536 8566 8638 9073 9304 9457 9475 9592
Size: 327 30 72 435 231 153 18 117
Cuts at: 9592 9646 9730 9784 9805 9976 10165 10606 11002
Size: 54 84 54 21 171 189 441 396
Cuts at: 11002 11116 11362 11395 11812 12069 12223 12283 12583
Size: 114 246 33 417 257 154 60 300
Cuts at: 12583 12913 13114 14935 15124 15181 15277 16089 17661
Size: 330 201 1821 189 57 96 812 1572
Cuts at: 17661 2536
Size: 3221
Fragments arranged by size:
3221 1821 1572 846 840 812 639 618
513 513 441 435 432 417 414 396
330 327 300 267 257 246 231 201
189 189 180 171 159 154 153 117
114 108 96 84 72 69 63 60
57 54 54 33 30 21 18 9
3
ApaI G_GGCC'C
Cuts at: 4502 5326 5416 6607 10286 11270 11515 11662 12263
Size: 824 90 1191 3679 984 245 147 601
Cuts at: 12263 12811 13269 13997 14840 15629 4502
Size: 548 458 728 843 789 7219
Fragments arranged by size:
7219 3679 1191 984 843 824 789 728
601 548 458 245 147 90
ApaLI G'TGCA_C
Cuts at: 4469 4929 5015 6441 10460 12437 16403 17559 4469
Size: 460 86 1426 4019 1977 3966 1156 5256
Fragments arranged by size:
5256 4019 3966 1977 1426 1156 460 86
ApoI r'AATT_y
Cuts at: 1493 3704 8519 15655 15881 16033 18142 1493
Size: 2211 4815 7136 226 152 2109 1697
Fragments arranged by size:
7136 4815 2211 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 16900 160
Size: 872 15868 1606
Fragments arranged by size:
15868 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18346
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2254 2271 3548 3762 4847 5378
Size: 73 93 218 17 1277 214 1085 531
Cuts at: 5378 5609 8292 8385 10286 10628 10707 11270 11382
Size: 231 2683 93 1901 342 79 563 112
Cuts at: 11382 11481 11655 12263 12325 12605 12794 12983 13034
Size: 99 174 608 62 280 189 189 51
Cuts at: 13034 13268 13997 14441 14713 15495 15577 15601 15671
Size: 234 729 444 272 782 82 24 70
Cuts at: 15671 1870
Size: 4545
Fragments arranged by size:
4545 2683 1901 1277 1085 782 729 608
563 531 444 342 280 272 234 231
218 214 189 189 174 112 99 93
93 82 79 73 70 62 51 24
17
BamHI G'GATC_C
Cuts at: 14756 15676 16043 14756
Size: 920 367 17059
Fragments arranged by size:
17059 920 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2337 2526 3073 3393 3543
Size: 325 217 627 550 189 547 320 150
Cuts at: 3543 3767 4576 4653 5663 6654 7112 7950 8070
Size: 224 809 77 1010 991 458 838 120
Cuts at: 8070 10945 11069 11192 11521 11598 13010 13593 13610
Size: 2875 124 123 329 77 1412 583 17
Cuts at: 13610 13796 14604 15290 15667 17122 618
Size: 186 808 686 377 1455 1842
Fragments arranged by size:
2875 1842 1455 1412 1010 991 838 809
808 686 627 583 550 547 458 377
329 325 320 224 217 189 186 150
124 123 120 77 77 17
BanII G_rGCy'C
Cuts at: 1391 4029 4502 4971 5326 5379 5416 5610 6290
Size: 2638 473 469 355 53 37 194 680
Cuts at: 6290 6607 8392 8694 9190 9270 10050 10286 10992
Size: 317 1785 302 496 80 780 236 706
Cuts at: 10992 11062 11270 11515 11589 11662 11676 11772 11955
Size: 70 208 245 74 73 14 96 183
Cuts at: 11955 11983 12041 12263 12811 13076 13269 13524 13902
Size: 28 58 222 548 265 193 255 378
Cuts at: 13902 13997 14840 15204 15629 15665 1391
Size: 95 843 364 425 36 4072
Fragments arranged by size:
4072 2638 1785 843 780 706 680 548
496 473 469 425 378 364 355 317
302 265 255 245 236 222 208 194
193 183 96 95 80 74 73 70
58 53 37 36 28 14
BbeI G_GCGC'C
Cuts at: 3547 5667 6658 7954 8074 14608 15294 3547
Size: 2120 991 1296 120 6534 686 6599
Fragments arranged by size:
6599 6534 2120 1296 991 686 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 3176 6773 7476 10068 12893 13810
Size: 971 315 962 3597 703 2592 2825 917
Cuts at: 13810 14396 928
Size: 586 4878
Fragments arranged by size:
4878 3597 2825 2592 971 962 917 703
586 315
BbvCI CC'TCA_GC
Cuts at: 2736 7326 7754 7962 8274 8985 9144 9201 9768
Size: 4590 428 208 312 711 159 57 567
Cuts at: 9768 11972 12651 12951 13568 2736
Size: 2204 679 300 617 7514
Fragments arranged by size:
7514 4590 2204 711 679 617 567 428
312 300 208 159 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4682 4716 5104 5138 6163 6197 9250
Size: 34 2745 34 388 34 1025 34 3053
Cuts at: 9250 9284 10078 10112 10960 10994 16537 16571 1903
Size: 34 794 34 848 34 5543 34 3678
Fragments arranged by size:
5543 3678 3053 2745 1025 848 794 388
34 34 34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 16238 17448 16238
Size: 1210 17136
BclI T'GATC_A
Cuts at: 1548 12858 14583 1548
Size: 11310 1725 5311
Fragments arranged by size:
11310 5311 1725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3002 3681 4364
Size: 107 122 71 631 605 1330 679 683
Cuts at: 4364 4604 5360 5390 5900 6320 6408 6584 6611
Size: 240 756 30 510 420 88 176 27
Cuts at: 6611 6869 6923 7274 7316 7409 8126 8267 8657
Size: 258 54 351 42 93 717 141 390
Cuts at: 8657 8870 9830 11265 11303 11519 12078 12155 12245
Size: 213 960 1435 38 216 559 77 90
Cuts at: 12245 12296 12747 13160 13415 15065 15119 16957 136
Size: 51 451 413 255 1650 54 1838 1525
Fragments arranged by size:
1838 1650 1525 1435 1330 960 756 717
683 679 631 605 559 510 451 420
413 390 351 258 255 240 216 213
176 141 122 107 93 90 88 77
71 54 54 51 42 38 30 27
BglII A'GATC_T
Cuts at: 2439 10359 15333 18341 2439
Size: 7920 4974 3008 2444
Fragments arranged by size:
7920 4974 3008 2444
BlpI GC'TnA_GC
Cuts at: 4529 4866 6057 6333 6834 8723 9150 9363 11624
Size: 337 1191 276 501 1889 427 213 2261
Cuts at: 11624 13296 15763 4529
Size: 1672 2467 7112
Fragments arranged by size:
7112 2467 2261 1889 1672 1191 501 427
337 276 213
Bme1580I G_kGCm'C
Cuts at: 1790 3078 3730 4473 4502 4560 4581 4933 5019
Size: 1288 652 743 29 58 21 352 86
Cuts at: 5019 5065 5326 5416 5473 6445 6607 6847 7429
Size: 46 261 90 57 972 162 240 582
Cuts at: 7429 8455 10286 10464 10479 11202 11270 11463 11515
Size: 1026 1831 178 15 723 68 193 52
Cuts at: 11515 11662 11935 12263 12441 12811 13013 13269 13997
Size: 147 273 328 178 370 202 256 728
Cuts at: 13997 14840 15225 15380 15629 16407 17563 1790
Size: 843 385 155 249 778 1156 2573
Fragments arranged by size:
2573 1831 1288 1156 1026 972 843 778
743 728 723 652 582 385 370 352
328 273 261 256 249 240 202 193
178 178 162 155 147 90 86 68
58 57 52 46 29 21 15
BmgBI CAC'GTC
Cuts at: 1318 2725 2806 3370 4711 5518 5593 10066 10486
Size: 1407 81 564 1341 807 75 4473 420
Cuts at: 10486 12463 15286 1318
Size: 1977 2823 4378
Fragments arranged by size:
4473 4378 2823 1977 1407 1341 807 564
420 81 75
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5627 11977 14049
Size: 416 53 83 399 24 4210 6350 2072
Cuts at: 14049 16077 17035 442
Size: 2028 958 1753
Fragments arranged by size:
6350 4210 2072 2028 1753 958 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 18346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 3994 4897 6574 7357 8713 11695 12055 2269
Size: 1725 903 1677 783 1356 2982 360 8560
Fragments arranged by size:
8560 2982 1725 1677 1356 903 783 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 2542 2584 2672 2914 3062 3158 3197
Size: 1025 278 42 88 242 148 96 39
Cuts at: 3197 3575 3869 3955 4019 4304 4640 4811 5012
Size: 378 294 86 64 285 336 171 201
Cuts at: 5012 5225 5246 5279 5789 5840 6131 6638 6725
Size: 213 21 33 510 51 291 507 87
Cuts at: 6725 6800 6917 7106 7220 7391 7664 7742 7856
Size: 75 117 189 114 171 273 78 114
Cuts at: 7856 7862 7925 8048 8609 9128 9152 9326 9389
Size: 6 63 123 561 519 24 174 63
Cuts at: 9389 9572 9752 9827 10214 10442 10628 10985 11117
Size: 183 180 75 387 228 186 357 132
Cuts at: 11117 11573 12110 12191 12419 12605 13196 13484 13925
Size: 456 537 81 228 186 591 288 441
Cuts at: 13925 14153 14531 14776 14891 14960 15188 15416 17006
Size: 228 378 245 115 69 228 228 1590
Cuts at: 17006 1239
Size: 2579
Fragments arranged by size:
2579 1590 1025 591 561 537 519 510
507 456 441 387 378 378 357 336
294 291 288 285 278 273 245 242
228 228 228 228 228 213 201 189
186 186 183 180 174 171 171 148
132 123 117 115 114 114 96 88
87 86 81 78 75 75 69 64
63 63 51 42 39 33 24 21
6
Bpu10I CC'TnA_GC
Cuts at: 2093 2736 5318 7326 7754 7962 8274 8391 8985
Size: 643 2582 2008 428 208 312 117 594
Cuts at: 8985 9144 9201 9768 11972 12651 12951 13568 13586
Size: 159 57 567 2204 679 300 617 18
Cuts at: 13586 2093
Size: 6853
Fragments arranged by size:
6853 2582 2204 2008 679 643 617 594
567 428 312 300 208 159 117 57
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3365 5252 5324 5579 5903 6896 7532 7556
Size: 312 1887 72 255 324 993 636 24
Cuts at: 7556 8717 8924 8963 9143 9401 11981 13118 15314
Size: 1161 207 39 180 258 2580 1137 2196
Cuts at: 15314 16471 17336 17634 3053
Size: 1157 865 298 3765
Fragments arranged by size:
3765 2580 2196 1887 1161 1157 1137 993
865 636 324 312 298 258 255 207
180 72 39 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2964 8588 11495 11693 11885 12309 12656
Size: 617 1465 5624 2907 198 192 424 347
Cuts at: 12656 17009 882
Size: 4353 2219
Fragments arranged by size:
5624 4353 2907 2219 1465 617 424 347
198 192
BsaAI yAC'GTr
Cuts at: 493 1204 3127 4666 5953 6805 10593 12570 12931
Size: 711 1923 1539 1287 852 3788 1977 361
Cuts at: 12931 13543 16137 493
Size: 612 2594 2702
Fragments arranged by size:
3788 2702 2594 1977 1923 1539 1287 852
711 612 361
BsaBI GATnn'nnATC
Cuts at: 2948 10880 12863 14549 18340 2948
Size: 7932 1983 1686 3791 2954
Fragments arranged by size:
7932 3791 2954 1983 1686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2784 3252 3544 5445
Size: 53 83 186 936 1251 468 292 1901
Cuts at: 5445 5664 5679 6655 7951 8071 9210 10713 11409
Size: 219 15 976 1296 120 1139 1503 696
Cuts at: 11409 11535 13641 13827 14393 14605 15006 15258 15291
Size: 126 2106 186 566 212 401 252 33
Cuts at: 15291 15525 16153 16535 17170 275
Size: 234 628 382 635 1451
Fragments arranged by size:
2106 1901 1503 1451 1296 1251 1139 976
936 696 635 628 566 468 401 382
292 252 234 219 212 186 186 126
120 83 53 33 15
BsaWI w'CCGG_w
Cuts at: 9222 13922 14901 14981 16779 17177 17451 17598 18009
Size: 4700 979 80 1798 398 274 147 411
Cuts at: 18009 9222
Size: 9559
Fragments arranged by size:
9559 4700 1798 979 411 398 274 147
80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 2275 2305 2540
Size: 30 165 30 816 30 26 30 235
Cuts at: 2540 2570 3095 3125 3878 3908 7130 7160 8081
Size: 30 525 30 753 30 3222 30 921
Cuts at: 8081 8111 10682 10712 11897 11927 12095 12125 12734
Size: 30 2571 30 1185 30 168 30 609
Cuts at: 12734 12764 13009 13039 14519 14549 14582 14612 14864
Size: 30 245 30 1480 30 33 30 252
Cuts at: 14864 14894 15161 15191 1178
Size: 30 267 30 4333
Fragments arranged by size:
4333 3222 2571 1480 1185 921 816 753
609 525 267 252 245 235 168 165
33 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2954 3245 3617 3814 3926 3929 3932 4778
Size: 1768 291 372 197 112 3 3 846
Cuts at: 4778 5324 5345 5786 5816 6053 6056 6059 6089
Size: 546 21 441 30 237 3 3 30
Cuts at: 6089 6215 6233 6278 6371 6374 6467 6500 6635
Size: 126 18 45 93 3 93 33 135
Cuts at: 6635 6656 6842 6893 6965 7019 7058 7217 7220
Size: 21 186 51 72 54 39 159 3
Cuts at: 7220 7343 7346 7421 7661 7694 7790 7793 7952
Size: 123 3 75 240 33 96 3 159
Cuts at: 7952 7955 7979 8009 8012 8075 8291 8300 8384
Size: 3 24 30 3 63 216 9 84
Cuts at: 8384 8606 8639 8699 8702 8732 8822 8852 8912
Size: 222 33 60 3 30 90 30 60
Cuts at: 8912 9095 9218 9251 9440 9614 9677 9707 9731
Size: 183 123 33 189 174 63 30 24
Cuts at: 9731 9770 9785 9824 9857 10019 10529 10910 11114
Size: 39 15 39 33 162 510 381 204
Cuts at: 11114 11513 11684 11897 12506 12698 12701 12812 13400
Size: 399 171 213 609 192 3 111 588
Cuts at: 13400 13703 13775 13982 14186 14240 14306 14545 14548
Size: 303 72 207 204 54 66 239 3
Cuts at: 14548 14555 14737 14752 14755 14843 15386 15562 15565
Size: 7 182 15 3 88 543 176 3
Cuts at: 15565 1186
Size: 3967
Fragments arranged by size:
3967 1768 846 609 588 546 543 510
441 399 381 372 303 291 240 239
237 222 216 213 207 204 204 197
192 189 186 183 182 176 174 171
162 159 159 135 126 123 123 112
111 96 93 93 90 88 84 75
72 72 66 63 63 60 60 54
54 51 45 39 39 39 33 33
33 33 33 30 30 30 30 30
30 24 24 21 21 18 15 15
9 7 3 3 3 3 3 3
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 3650 3667 4037 4399 4626 5064 5110 5215 5550
Size: 17 370 362 227 438 46 105 335
Cuts at: 5550 7692 7846 9213 9585 10541 11412 11441 11555
Size: 2142 154 1367 372 956 871 29 114
Cuts at: 11555 12034 12518 12810 13410 13523 14799 14975 17549
Size: 479 484 292 600 113 1276 176 2574
Cuts at: 17549 18045 3650
Size: 496 3951
Fragments arranged by size:
3951 2574 2142 1367 1276 956 871 600
496 484 479 438 372 370 362 335
292 227 176 154 114 113 105 46
29 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2414 2828 3107 3392 3419 3557
Size: 573 417 261 414 279 285 27 138
Cuts at: 3557 3671 4400 4488 4733 4766 4781 5670 6533
Size: 114 729 88 245 33 15 889 863
Cuts at: 6533 6744 7994 8198 8318 8729 8981 9239 9317
Size: 211 1250 204 120 411 252 258 78
Cuts at: 9317 9461 10277 13259 14938 15194 1163
Size: 144 816 2982 1679 256 4315
Fragments arranged by size:
4315 2982 1679 1250 889 863 816 729
573 417 414 411 285 279 261 258
256 252 245 211 204 144 138 120
114 88 78 33 27 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2575 2904 3943 7387 7818 7992
Size: 275 186 574 329 1039 3444 431 174
Cuts at: 7992 10314 10984 12838 13282 14025 14593 14650 15114
Size: 2322 670 1854 444 743 568 57 464
Cuts at: 15114 15589 15796 16557 17585 1540
Size: 475 207 761 1028 2301
Fragments arranged by size:
3444 2322 2301 1854 1039 1028 761 743
670 574 568 475 464 444 431 329
275 207 186 174 57
BsiHKAI G_wGCw'C
Cuts at: 2094 4029 4473 4530 4933 4971 5019 5319 5379
Size: 1935 444 57 403 38 48 300 60
Cuts at: 5379 5610 6290 6445 8694 9270 9936 10050 10407
Size: 231 680 155 2249 576 666 114 357
Cuts at: 10407 10464 10992 11589 11676 11772 11955 12441 13902
Size: 57 528 597 87 96 183 486 1461
Cuts at: 13902 16407 16492 17563 2094
Size: 2505 85 1071 2877
Fragments arranged by size:
2877 2505 2249 1935 1461 1071 680 666
597 576 528 486 444 403 357 300
231 183 155 114 96 87 85 60
57 57 48 38
BsiWI C'GTAC_G
Cuts at: 5963 5963
Size: 18346
BsmI GAATG_Cn'
Cuts at: 6255 15857 15950 6255
Size: 9602 93 8651
Fragments arranged by size:
9602 8651 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2562 2773 4997 6701 7160 9077 9458 10625 11022
Size: 211 2224 1704 459 1917 381 1167 397
Cuts at: 11022 11350 12602 14438 14794 14825 14858 2562
Size: 328 1252 1836 356 31 33 6050
Fragments arranged by size:
6050 2224 1917 1836 1704 1252 1167 459
397 381 356 328 211 33 31
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 3078 3730 4029 4473 4502 4530
Size: 399 304 984 652 299 444 29 28
Cuts at: 4530 4560 4581 4933 4971 5019 5065 5319 5326
Size: 30 21 352 38 48 46 254 7
Cuts at: 5326 5379 5416 5473 5610 6290 6445 6607 6847
Size: 53 37 57 137 680 155 162 240
Cuts at: 6847 7429 8392 8455 8694 9190 9270 9936 10050
Size: 582 963 63 239 496 80 666 114
Cuts at: 10050 10286 10407 10464 10479 10992 11062 11202 11270
Size: 236 121 57 15 513 70 140 68
Cuts at: 11270 11463 11515 11589 11662 11676 11772 11935 11955
Size: 193 52 74 73 14 96 163 20
Cuts at: 11955 11983 12041 12263 12441 12811 13013 13076 13269
Size: 28 58 222 178 370 202 63 193
Cuts at: 13269 13524 13902 13997 14840 15204 15225 15380 15629
Size: 255 378 95 843 364 21 155 249
Cuts at: 15629 15665 16407 16492 17563 1391
Size: 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 984 963 843 742 680 666
652 582 513 496 444 399 378 370
364 352 304 299 255 254 249 240
239 236 222 202 193 193 178 163
162 155 155 140 137 121 114 96
95 85 80 74 73 70 68 63
63 58 57 57 53 52 48 46
38 37 36 30 29 28 28 21
21 20 15 14 7
BspEI T'CCGG_A
Cuts at: 9222 18009 9222
Size: 8787 9559
BspHI T'CATG_A
Cuts at: 11278 16235 17965 11278
Size: 4957 1730 11659
Fragments arranged by size:
11659 4957 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2910 3585 3594 3654 3807 4299 4444 5221
Size: 2066 675 9 60 153 492 145 777
Cuts at: 5221 5967 6499 6679 7759 8323 8542 8622 8818
Size: 746 532 180 1080 564 219 80 196
Cuts at: 8818 9850 10086 10605 10833 11154 12661 13005 13101
Size: 1032 236 519 228 321 1507 344 96
Cuts at: 13101 13696 14316 15320 15701 844
Size: 595 620 1004 381 3489
Fragments arranged by size:
3489 2066 1507 1080 1032 1004 777 746
675 620 595 564 532 519 492 381
344 321 236 228 219 196 180 153
145 96 80 60 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2122 2686 3050 3142 3724 4725 5512
Size: 429 299 564 364 92 582 1001 787
Cuts at: 5512 5542 6085 6166 6652 6838 6868 6889 7501
Size: 30 543 81 486 186 30 21 612
Cuts at: 7501 7726 7945 8041 8087 8350 9118 11638 12589
Size: 225 219 96 46 263 768 2520 951
Cuts at: 12589 13225 13300 14386 14598 14652 14736 16233 1394
Size: 636 75 1086 212 54 84 1497 3507
Fragments arranged by size:
3507 2520 1497 1086 1001 951 787 768
636 612 582 564 543 486 429 364
299 263 225 219 212 186 96 92
84 81 75 54 46 30 30 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 16841 17015 65
Size: 1183 15593 174 1396
Fragments arranged by size:
15593 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2598 2637 4044 4854 5047 5359 5887
Size: 110 788 39 1407 810 193 312 528
Cuts at: 5887 6342 7382 7386 9986 10239 10494 10914 11189
Size: 455 1040 4 2600 253 255 420 275
Cuts at: 11189 11569 11601 12077 12305 12833 13310 13421 13613
Size: 380 32 476 228 528 477 111 192
Cuts at: 13613 13922 14181 14675 14723 14981 15152 15185 15476
Size: 309 259 494 48 258 171 33 291
Cuts at: 15476 15488 15500 15530 16990 17177 1700
Size: 12 12 30 1460 187 2869
Fragments arranged by size:
2869 2600 1460 1407 1040 810 788 528
528 494 477 476 455 420 380 312
309 291 275 259 258 255 253 228
193 192 187 171 111 110 48 39
33 32 30 12 12 4
BsrGI T'GTAC_A
Cuts at: 96 12675 96
Size: 12579 5767
BssHII G'CGCG_C
Cuts at: 1925 6587 6872 10318 14029 1925
Size: 4662 285 3446 3711 6242
Fragments arranged by size:
6242 4662 3711 3446 285
BssSI C'ACGA_G
Cuts at: 3437 5327 10580 12233 12272 13259 16406 17418 3437
Size: 1890 5253 1653 39 987 3147 1012 4365
Fragments arranged by size:
5253 4365 3147 1890 1653 1012 987 39
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3535 3586 4225 4660 4909 5023 5575 5764
Size: 2741 51 639 435 249 114 552 189
Cuts at: 5764 6193 6277 6436 6440 8209 8227 8536 8959
Size: 429 84 159 4 1769 18 309 423
Cuts at: 8959 9439 9457 9475 11252 11395 12069 13803 14935
Size: 480 18 18 1777 143 674 1734 1132
Cuts at: 14935 794
Size: 4205
Fragments arranged by size:
4205 2741 1777 1769 1734 1132 674 639
552 480 435 429 423 309 249 189
159 143 114 84 51 18 18 18
4
BstBI TT'CG_AA
Cuts at: 17150 17150
Size: 18346
BstEII G'GTnAC_C
Cuts at: 1184 9286 10768 11839 12841 13306 13765 1184
Size: 8102 1482 1071 1002 465 459 5765
Fragments arranged by size:
8102 5765 1482 1071 1002 465 459
BstXI CCAn_nnnn'nTGG
Cuts at: 2459 4037 5257 5281 5716 2459
Size: 1578 1220 24 435 15089
Fragments arranged by size:
15089 1578 1220 435 24
BstYI r'GATC_y
Cuts at: 1972 2439 2949 5700 6300 6363 7782 9309 10359
Size: 467 510 2751 600 63 1419 1527 1050
Cuts at: 10359 14238 14756 15333 15676 16043 16428 16445 17897
Size: 3879 518 577 343 367 385 17 1452
Cuts at: 17897 17983 17995 18341 1972
Size: 86 12 346 1977
Fragments arranged by size:
3879 2751 1977 1527 1452 1419 1050 600
577 518 510 467 385 367 346 343
86 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 2718 3839 10210 14169 2718
Size: 1121 6371 3959 6895
Fragments arranged by size:
6895 6371 3959 1121
BtgI C'CryG_G
Cuts at: 513 1065 4077 5290 5785 7134 8652 9912 10035
Size: 552 3012 1213 495 1349 1518 1260 123
Cuts at: 10035 10437 10479 11166 11235 11421 11730 11844 12414
Size: 402 42 687 69 186 309 114 570
Cuts at: 12414 12742 13077 13660 13683 13722 14116 14148 14190
Size: 328 335 583 23 39 394 32 42
Cuts at: 14190 14841 15511 15639 513
Size: 651 670 128 3220
Fragments arranged by size:
3220 3012 1518 1349 1260 1213 687 670
651 583 570 552 495 402 394 335
328 309 186 128 123 114 69 42
42 39 32 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2232 2362 2554 5056 5157 9343
Size: 683 56 1006 130 192 2502 101 4186
Cuts at: 9343 11065 13792 14614 487
Size: 1722 2727 822 4219
Fragments arranged by size:
4219 4186 2727 2502 1722 1006 822 683
192 130 101 56
BtsI GCAGTG_nn'
Cuts at: 4118 4235 6434 6727 8573 9849 11253 11790 12070
Size: 117 2199 293 1846 1276 1404 537 280
Cuts at: 12070 15862 16656 16676 4118
Size: 3792 794 20 5788
Fragments arranged by size:
5788 3792 2199 1846 1404 1276 794 537
293 280 117 20
ClaI AT'CG_AT
Cuts at: 1554 14619 1554
Size: 13065 5281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3492 3527 14707 14742 529
Size: 35 2928 35 11180 35 4133
Fragments arranged by size:
11180 4133 2928 35 35 35
DraI TTT'AAA
Cuts at: 15650 15650
Size: 18346
DraIII CAC_nnn'GTG
Cuts at: 2154 3619 3734 4650 6440 10474 11458 12451 2154
Size: 1465 115 916 1790 4034 984 993 8049
Fragments arranged by size:
8049 4034 1790 1465 993 984 916 115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2729 14570 15106 17353 809
Size: 507 1413 11841 536 2247 1802
Fragments arranged by size:
11841 2247 1802 1413 536 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2221 2481 2572
Size: 54 1425 50 275 18 391 260 91
Cuts at: 2572 2901 3129 4356 4434 4737 5361 5718 5901
Size: 329 228 1227 78 303 624 357 183
Cuts at: 5901 6090 6129 7131 7275 7308 7384 7401 7461
Size: 189 39 1002 144 33 76 17 60
Cuts at: 7461 7521 7815 7989 9171 9576 9651 9889 10311
Size: 60 294 174 1182 405 75 238 422
Cuts at: 10311 10351 11163 11571 12183 12835 13416 13615 13917
Size: 40 812 408 612 652 581 199 302
Cuts at: 13917 14022 15111 15154 15586 15642 15793 16682 18311
Size: 105 1089 43 432 56 151 889 1629
Cuts at: 18311 8
Size: 43
Fragments arranged by size:
1629 1425 1227 1182 1089 1002 889 812
652 624 612 581 432 422 408 405
391 357 329 303 302 294 275 260
238 228 199 189 183 174 151 144
105 91 78 76 75 60 60 56
54 50 43 43 40 39 33 18
17
EagI C'GGCC_G
Cuts at: 1537 1812 2572 2901 7384 7815 7989 10311 12835
Size: 275 760 329 4483 431 174 2322 2524
Cuts at: 12835 14022 15111 15586 15793 1537
Size: 1187 1089 475 207 4090
Fragments arranged by size:
4483 4090 2524 2322 1187 1089 760 475
431 329 275 207 174
EarI CTCTTCn'nnn_
Cuts at: 1353 2585 3365 4670 4932 6962 7431 7973 8148
Size: 1232 780 1305 262 2030 469 542 175
Cuts at: 8148 8334 8699 9566 10373 12350 12702 13355 14084
Size: 186 365 867 807 1977 352 653 729
Cuts at: 14084 14222 15562 16276 1353
Size: 138 1340 714 3423
Fragments arranged by size:
3423 2030 1977 1340 1305 1232 867 807
780 729 714 653 542 469 365 352
262 186 175 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2696 3353 4255 6176 6311 6464
Size: 1064 138 1107 657 902 1921 135 153
Cuts at: 6464 6473 6557 6653 6662 6746 6962 7025 7055
Size: 9 84 96 9 84 216 63 30
Cuts at: 7055 7121 7448 7511 7574 7601 7628 7844 7949
Size: 66 327 63 63 27 27 216 105
Cuts at: 7949 8165 8267 8297 8318 8474 8660 8849 9005
Size: 216 102 30 21 156 186 189 156
Cuts at: 9005 9092 9248 9970 10403 10550 12380 12527 12656
Size: 87 156 722 433 147 1830 147 129
Cuts at: 12656 12890 13036 13055 13868 14114 16933 17307 17453
Size: 234 146 19 813 246 2819 374 146
Cuts at: 17453 387
Size: 1280
Fragments arranged by size:
2819 1921 1830 1280 1107 1064 902 813
722 657 433 374 327 246 234 216
216 216 189 186 156 156 156 153
147 147 146 146 138 135 129 105
102 96 87 84 84 66 63 63
63 30 30 27 27 21 19 9
9
EcoICRI GAG'CTC
Cuts at: 4027 4969 5377 5608 6288 8692 9268 10048 10990
Size: 942 408 231 680 2404 576 780 942
Cuts at: 10990 11587 11674 11770 11953 13900 4027
Size: 597 87 96 183 1947 8473
Fragments arranged by size:
8473 2404 1947 942 942 780 680 597
576 408 231 183 96 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2542 2584 2672 2864 2914 2990
Size: 393 632 278 42 88 192 50 76
Cuts at: 2990 3062 3158 3170 3197 3500 3575 3869 3955
Size: 72 96 12 27 303 75 294 86
Cuts at: 3955 4019 4025 4178 4304 4406 4640 4775 4811
Size: 64 6 153 126 102 234 135 36
Cuts at: 4811 5012 5225 5246 5279 5420 5789 5840 6131
Size: 201 213 21 33 141 369 51 291
Cuts at: 6131 6638 6725 6800 6917 7106 7220 7391 7664
Size: 507 87 75 117 189 114 171 273
Cuts at: 7664 7742 7814 7856 7862 7925 8048 8375 8609
Size: 78 72 42 6 63 123 327 234
Cuts at: 8609 8660 9128 9152 9326 9389 9572 9752 9827
Size: 51 468 24 174 63 183 180 75
Cuts at: 9827 10133 10214 10442 10628 10985 11117 11156 11384
Size: 306 81 228 186 357 132 39 228
Cuts at: 11384 11573 11902 12110 12191 12419 12469 12605 13196
Size: 189 329 208 81 228 50 136 591
Cuts at: 13196 13484 13925 13973 14129 14153 14387 14531 14776
Size: 288 441 48 156 24 234 144 245
Cuts at: 14776 14891 14960 15146 15188 15416 16409 17006 17793
Size: 115 69 186 42 228 993 597 787
Cuts at: 17793 18061 1239
Size: 268 1524
Fragments arranged by size:
1524 993 787 632 597 591 507 468
441 393 369 357 329 327 306 303
294 291 288 278 273 268 245 234
234 234 228 228 228 228 213 208
201 192 189 189 186 186 183 180
174 171 156 153 144 141 136 135
132 126 123 117 115 114 102 96
88 87 86 81 81 78 76 75
75 75 72 72 69 64 63 63
51 51 50 50 48 42 42 42
39 36 33 27 24 24 21 12
6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2174 2243 2663 4295 4922 5768 6662 6794 6977
Size: 69 420 1632 627 846 894 132 183
Cuts at: 6977 8804 9350 10193 10421 11354 11366 11495 11900
Size: 1827 546 843 228 933 12 129 405
Cuts at: 11900 12170 12347 12398 12767 12962 13025 13493 14081
Size: 270 177 51 369 195 63 468 588
Cuts at: 14081 14132 15128 15230 2174
Size: 51 996 102 5290
Fragments arranged by size:
5290 1827 1632 996 933 894 846 843
627 588 546 468 420 405 369 270
228 195 183 177 132 129 102 69
63 51 51 12
EcoO109I rG'GnC_Cy
Cuts at: 2041 2097 2248 2533 2689 3583 3843 4288 4499
Size: 56 151 285 156 894 260 445 211
Cuts at: 4499 4636 5322 5761 6604 6613 8902 9472 10283
Size: 137 686 439 843 9 2289 570 811
Cuts at: 10283 10339 10387 10624 10822 10930 11267 11323 11359
Size: 56 48 237 198 108 337 56 36
Cuts at: 11359 11371 11392 11511 11512 11725 11968 12022 12136
Size: 12 21 119 1 213 243 54 114
Cuts at: 12136 12260 12364 12601 12955 13255 13265 13390 13744
Size: 124 104 237 354 300 10 125 354
Cuts at: 13744 13994 14050 14098 14485 14796 14837 15019 15085
Size: 250 56 48 387 311 41 182 66
Cuts at: 15085 15121 15235 15606 15625 15626 2041
Size: 36 114 371 19 1 4761
Fragments arranged by size:
4761 2289 894 843 811 686 570 445
439 387 371 354 354 337 311 300
285 260 250 243 237 237 213 211
198 182 156 151 137 125 124 119
114 114 108 104 66 56 56 56
56 54 48 48 41 36 36 21
19 12 10 9 1 1
EcoRI G'AATT_C
Cuts at: 3704 15655 16033 3704
Size: 11951 378 6017
Fragments arranged by size:
11951 6017 378
EcoRV GAT'ATC
Cuts at: 2449 2449
Size: 18346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 7819 10579 12556 13672 14290 2161
Size: 5658 2760 1977 1116 618 6217
Fragments arranged by size:
6217 5658 2760 1977 1116 618
FseI GG_CCGG'CC
Cuts at: 7386 12837 15156 7386
Size: 5451 2319 10576
Fragments arranged by size:
10576 5451 2319
FspI TGC'GCA
Cuts at: 6350 7700 7883 8420 9021 10937 13535 14414 16852
Size: 1350 183 537 601 1916 2598 879 2438
Cuts at: 16852 6350
Size: 7844
Fragments arranged by size:
7844 2598 2438 1916 1350 879 601 537
183
HaeII r_GCGC'y
Cuts at: 1678 3445 3547 3630 3986 4774 4957 5533 5566
Size: 1767 102 83 356 788 183 576 33
Cuts at: 5566 5667 5785 6403 6499 6658 6787 6904 6929
Size: 101 118 618 96 159 129 117 25
Cuts at: 6929 7129 7600 7687 7793 7954 8027 8074 8797
Size: 200 471 87 106 161 73 47 723
Cuts at: 8797 9144 9157 9220 9346 9526 10615 11020 11226
Size: 347 13 63 126 180 1089 405 206
Cuts at: 11226 12223 13567 13730 14422 14608 14992 15163 15294
Size: 997 1344 163 692 186 384 171 131
Cuts at: 15294 17493 1678
Size: 2199 2531
Fragments arranged by size:
2531 2199 1767 1344 1089 997 788 723
692 618 576 471 405 384 356 347
206 200 186 183 180 171 163 161
159 131 129 126 118 117 106 102
101 96 87 83 73 63 47 33
25 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2735 2767 7319 7351 8081
Size: 32 135 32 1163 32 4552 32 730
Cuts at: 8081 8113 11897 11929 12095 12127 12308 12340 12964
Size: 32 3784 32 166 32 181 32 624
Cuts at: 12964 12996 13028 13060 13499 13531 14042 14074 14415
Size: 32 32 32 439 32 511 32 341
Cuts at: 14415 14447 14582 14614 14696 14728 14864 14896 16987
Size: 32 135 32 82 32 136 32 2091
Cuts at: 16987 17019 17061 17093 1373
Size: 32 42 32 2626
Fragments arranged by size:
4552 3784 2626 2091 1163 730 624 511
439 341 181 166 136 135 135 82
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 10255 15013 15690 15936 669
Size: 988 281 223 8094 4758 677 246 3079
Fragments arranged by size:
8094 4758 3079 988 677 281 246 223
HindIII A'AGCT_T
Cuts at: 748 9484 748
Size: 8736 9610
HpaI GTT'AAC
Cuts at: 15936 15936
Size: 18346
KasI G'GCGC_C
Cuts at: 3543 5663 6654 7950 8070 14604 15290 3543
Size: 2120 991 1296 120 6534 686 6599
Fragments arranged by size:
6599 6534 2120 1296 991 686 120
KpnI G_GTAC'C
Cuts at: 2530 15671 2530
Size: 13141 5205
MfeI C'AATT_G
Cuts at: 15945 15945
Size: 18346
MluI A'CGCG_T
Cuts at: 18222 18222
Size: 18346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2448 3246 6366 9415 11157 11181 11385 12762
Size: 1143 798 3120 3049 1742 24 204 1377
Cuts at: 12762 13617 17460 17644 1305
Size: 855 3843 184 2007
Fragments arranged by size:
3843 3120 3049 2007 1742 1377 1143 855
798 204 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2223 3131 4358 4739 7277
Size: 54 1425 343 391 908 1227 381 2538
Cuts at: 7277 7310 9578 9653 10353 13418 10
Size: 33 2268 75 700 3065 4938
Fragments arranged by size:
4938 3065 2538 2268 1425 1227 908 700
391 381 343 75 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 2193 2621 3953 4476 5056 10037 10886
Size: 648 1027 428 1332 523 580 4981 849
Cuts at: 10886 10889 11165 12671 16304 16663 16822 518
Size: 3 276 1506 3633 359 159 2042
Fragments arranged by size:
4981 3633 2042 1506 1332 1027 849 648
580 523 428 359 276 159 3
MspA1I CmG'CkG
Cuts at: 1606 2562 2839 3094 3238 3304 3667 4132 4324
Size: 956 277 255 144 66 363 465 192
Cuts at: 4324 4384 4492 4906 4990 5035 5292 5500 5554
Size: 60 108 414 84 45 257 208 54
Cuts at: 5554 5629 5716 5746 5860 6067 6121 6127 6277
Size: 75 87 30 114 207 54 6 150
Cuts at: 6277 6601 6778 6820 7063 7096 7102 7136 7189
Size: 324 177 42 243 33 6 34 53
Cuts at: 7189 7195 7453 7459 7486 7633 7696 7762 7820
Size: 6 258 6 27 147 63 66 58
Cuts at: 7820 7921 7927 8014 8110 8116 8239 8492 8587
Size: 101 6 87 96 6 123 253 95
Cuts at: 8587 8635 8890 8917 9073 9100 9124 9130 9454
Size: 48 255 27 156 27 24 6 324
Cuts at: 9454 9517 9592 9610 9643 9730 9742 9796 9914
Size: 63 75 18 33 87 12 54 118
Cuts at: 9914 10015 10162 10816 11002 11416 11596 11614 11803
Size: 101 147 654 186 414 180 18 189
Cuts at: 11803 11922 12280 12580 12805 12814 13414 13513 13582
Size: 119 358 300 225 9 600 99 69
Cuts at: 13582 13621 13735 13803 14314 14365 14803 14932 15447
Size: 39 114 68 511 51 438 129 515
Cuts at: 15447 15464 15513 15762 16439 17587 17832 1606
Size: 17 49 249 677 1148 245 2120
Fragments arranged by size:
2120 1148 956 677 654 600 515 511
465 438 414 414 363 358 324 324
300 277 258 257 255 255 253 249
245 243 225 208 207 192 189 186
180 177 156 150 147 147 144 129
123 119 118 114 114 108 101 101
99 96 95 87 87 87 84 75
75 69 68 66 66 63 63 60
58 54 54 54 53 51 49 48
45 42 39 34 33 33 30 27
27 27 24 18 18 17 12 9
6 6 6 6 6 6 6
NaeI GCC'GGC
Cuts at: 5049 5361 6344 7384 9988 10916 11191 11571 11603
Size: 312 983 1040 2604 928 275 380 32
Cuts at: 11603 12079 12835 14677 15154 15502 5049
Size: 476 756 1842 477 348 7893
Fragments arranged by size:
7893 2604 1842 1040 983 928 756 477
476 380 348 312 275 32
NarI GG'CG_CC
Cuts at: 3544 5664 6655 7951 8071 14605 15291 3544
Size: 2120 991 1296 120 6534 686 6599
Fragments arranged by size:
6599 6534 2120 1296 991 686 120
NcoI C'CATG_G
Cuts at: 513 1065 13683 14116 513
Size: 552 12618 433 4743
Fragments arranged by size:
12618 4743 552 433
NdeI CA'TA_TG
Cuts at: 387 387
Size: 18346
NgoMIV G'CCGG_C
Cuts at: 5047 5359 6342 7382 9986 10914 11189 11569 11601
Size: 312 983 1040 2604 928 275 380 32
Cuts at: 11601 12077 12833 14675 15152 15500 5047
Size: 476 756 1842 477 348 7893
Fragments arranged by size:
7893 2604 1842 1040 983 928 756 477
476 380 348 312 275 32
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 18346
NotI GC'GGCC_GC
Cuts at: 7815 15793 7815
Size: 7978 10368
NspI r_CATG'y
Cuts at: 1132 1229 6196 14710 15704 17249 1132
Size: 97 4967 8514 994 1545 2229
Fragments arranged by size:
8514 4967 2229 1545 994 97
PasI CC'CwG_GG
Cuts at: 1348 15619 1348
Size: 14271 4075
PciI A'CATG_T
Cuts at: 17245 17245
Size: 18346
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3040 3094 4495 4639 12817 1108
Size: 1932 54 1401 144 8178 6637
Fragments arranged by size:
8178 6637 1932 1401 144 54
PfoI T'CCnGG_A
Cuts at: 2709 3135 5370 5943 9564 9804 10398 13002 13788
Size: 426 2235 573 3621 240 594 2604 786
Cuts at: 13788 13902 14792 14853 15165 2709
Size: 114 890 61 312 5890
Fragments arranged by size:
5890 3621 2604 2235 890 786 594 573
426 312 240 114 61
PmeI GTTT'AAAC
Cuts at: 15650 15650
Size: 18346
PmlI CAC'GTG
Cuts at: 3127 4666 6805 3127
Size: 1539 2139 14668
Fragments arranged by size:
14668 2139 1539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12944 12976 16415 16447 17928 17960 12944
Size: 32 3439 32 1481 32 13330
Fragments arranged by size:
13330 3439 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2689 3583 3843 4288 8902 10339 10624 10822
Size: 441 894 260 445 4614 1437 285 198
Cuts at: 10822 11323 11725 11968 12022 12601 12955 14050 14796
Size: 501 402 243 54 579 354 1095 746
Cuts at: 14796 15085 15606 2248
Size: 289 521 4988
Fragments arranged by size:
4988 4614 1437 1095 894 746 579 521
501 445 441 402 354 289 285 260
243 198 54
PshAI GACnn'nnGTC
Cuts at: 1613 12014 1613
Size: 10401 7945
PsiI TTA'TAA
Cuts at: 15916 15916
Size: 18346
PspOMI G'GGCC_C
Cuts at: 4498 5322 5412 6603 10282 11266 11511 11658 12259
Size: 824 90 1191 3679 984 245 147 601
Cuts at: 12259 12807 13265 13993 14836 15625 4498
Size: 548 458 728 843 789 7219
Fragments arranged by size:
7219 3679 1191 984 843 824 789 728
601 548 458 245 147 90
PspXI vC'TCGA_Gb
Cuts at: 5378 5609 5378
Size: 231 18115
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2618 2650 4157 4189 5993 6025 10577 10609 12554
Size: 32 1507 32 1804 32 4552 32 1945
Cuts at: 12554 12586 14288 14320 2618
Size: 32 1702 32 6644
Fragments arranged by size:
6644 4552 1945 1804 1702 1507 32 32
32 32 32 32
PstI C_TGCA'G
Cuts at: 830 2307 2838 3210 3264 3289 3531 3582 3804
Size: 1477 531 372 54 25 242 51 222
Cuts at: 3804 4107 4230 4299 4889 4914 4989 5496 5676
Size: 303 123 69 590 25 75 507 180
Cuts at: 5676 5751 6186 6273 6384 6432 6612 6636 6741
Size: 75 435 87 111 48 180 24 105
Cuts at: 6741 6795 6945 7224 7240 8115 8148 8214 8410
Size: 54 150 279 16 875 33 66 196
Cuts at: 8410 8586 8634 8805 9003 9045 9414 9462 9480
Size: 176 48 171 198 42 369 48 18
Cuts at: 9480 9726 9801 9981 10014 10083 10155 10602 11007
Size: 246 75 180 33 69 72 447 405
Cuts at: 11007 11691 12060 13734 14313 15243 15698 830
Size: 684 369 1674 579 930 455 3478
Fragments arranged by size:
3478 1674 1477 930 875 684 590 579
531 507 455 447 435 405 372 369
369 303 279 246 242 222 198 196
180 180 180 176 171 150 123 111
105 87 75 75 75 72 69 69
66 54 54 51 48 48 48 42
33 33 25 25 24 18 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 18346
PvuII CAG'CTG
Cuts at: 2562 2839 3238 3667 4132 4324 4384 4492 4906
Size: 277 399 429 465 192 60 108 414
Cuts at: 4906 4990 5554 5629 5746 5860 6127 6277 6778
Size: 84 564 75 117 114 267 150 501
Cuts at: 6778 6820 7102 7195 7459 7696 7927 8110 8116
Size: 42 282 93 264 237 231 183 6
Cuts at: 8116 8239 8492 8587 8890 9073 9130 9454 9592
Size: 123 253 95 303 183 57 324 138
Cuts at: 9592 9730 9796 10162 11002 11416 11614 12280 12580
Size: 138 66 366 840 414 198 666 300
Cuts at: 12580 12814 13414 13513 13582 13621 13735 13803 14314
Size: 234 600 99 69 39 114 68 511
Cuts at: 14314 14803 2562
Size: 489 6105
Fragments arranged by size:
6105 840 666 600 564 511 501 489
465 429 414 414 399 366 324 303
300 282 277 267 264 253 237 234
231 198 192 183 183 150 138 138
123 117 114 114 108 99 95 93
84 75 69 68 66 60 57 42
39 6
SacI G_AGCT'C
Cuts at: 4029 4971 5379 5610 6290 8694 9270 10050 10992
Size: 942 408 231 680 2404 576 780 942
Cuts at: 10992 11589 11676 11772 11955 13902 4029
Size: 597 87 96 183 1947 8473
Fragments arranged by size:
8473 2404 1947 942 942 780 680 597
576 408 231 183 96 87
SacII CC_GC'GG
Cuts at: 5293 7137 9915 15514 5293
Size: 1844 2778 5599 8125
Fragments arranged by size:
8125 5599 2778 1844
SalI G'TCGA_C
Cuts at: 1936 15688 1936
Size: 13752 4594
SanDI GG'GwC_CC
Cuts at: 11323 11323
Size: 18346
SapI GCTCTTCn'nnn_
Cuts at: 3365 4670 6962 7973 8699 9566 13355 3365
Size: 1305 2292 1011 726 867 3789 8356
Fragments arranged by size:
8356 3789 2292 1305 1011 867 726
SbfI CC_TGCA'GG
Cuts at: 3582 6612 6795 8805 10083 15698 3582
Size: 3030 183 2010 1278 5615 6230
Fragments arranged by size:
6230 5615 3030 2010 1278 183
ScaI AGT'ACT
Cuts at: 11786 16594 18306 11786
Size: 4808 1712 11826
Fragments arranged by size:
11826 4808 1712
SexAI A'CCwGG_T
Cuts at: 14907 14907
Size: 18346
SfcI C'TryA_G
Cuts at: 826 1045 2303 2834 3206 3260 3285 3527 3578
Size: 219 1258 531 372 54 25 242 51
Cuts at: 3578 3800 4103 4127 4226 4295 4885 4910 4985
Size: 222 303 24 99 69 590 25 75
Cuts at: 4985 5492 5672 5747 5971 6182 6269 6380 6428
Size: 507 180 75 224 211 87 111 48
Cuts at: 6428 6608 6632 6737 6791 6941 7220 7236 8111
Size: 180 24 105 54 150 279 16 875
Cuts at: 8111 8144 8198 8210 8406 8582 8630 8801 8999
Size: 33 54 12 196 176 48 171 198
Cuts at: 8999 9041 9410 9458 9476 9512 9722 9797 9977
Size: 42 369 48 18 36 210 75 180
Cuts at: 9977 10010 10079 10151 10598 11003 11545 11687 12056
Size: 33 69 72 447 405 542 142 369
Cuts at: 12056 12310 13730 14309 15239 15427 15436 15694 16829
Size: 254 1420 579 930 188 9 258 1135
Cuts at: 16829 17510 17701 826
Size: 681 191 1471
Fragments arranged by size:
1471 1420 1258 1135 930 875 681 590
579 542 531 507 447 405 372 369
369 303 279 258 254 242 224 222
219 211 210 198 196 191 188 180
180 180 176 171 150 142 111 105
99 87 75 75 75 72 69 69
54 54 54 51 48 48 48 42
36 33 33 25 25 24 24 18
16 12 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 4364 4604 5360 5390 5900 6611 7409 8126 8657
Size: 240 756 30 510 711 798 717 531
Cuts at: 8657 11265 15065 15119 4364
Size: 2608 3800 54 7591
Fragments arranged by size:
7591 3800 2608 798 756 717 711 531
510 240 54 30
SfoI GGC'GCC
Cuts at: 3545 5665 6656 7952 8072 14606 15292 3545
Size: 2120 991 1296 120 6534 686 6599
Fragments arranged by size:
6599 6534 2120 1296 991 686 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18346
SgrAI Cr'CCGG_yG
Cuts at: 1700 13421 14723 14981 1700
Size: 11721 1302 258 5065
Fragments arranged by size:
11721 5065 1302 258
SmaI CCC'GGG
Cuts at: 1872 2273 3764 8294 8387 10709 11384 15497 15673
Size: 401 1491 4530 93 2322 675 4113 176
Cuts at: 15673 1872
Size: 4545
Fragments arranged by size:
4545 4530 4113 2322 1491 675 401 176
93
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3068 3380 5267 5339 5378 5558
Size: 197 926 1125 312 1887 72 39 180
Cuts at: 5558 5609 5882 6911 7547 7571 8732 8939 8978
Size: 51 273 1029 636 24 1161 207 39
Cuts at: 8978 9158 9416 11996 12983 13034 13133 15329 16450
Size: 180 258 2580 987 51 99 2196 1121
Cuts at: 16450 17351 17613 820
Size: 901 262 1553
Fragments arranged by size:
2580 2196 1887 1553 1161 1125 1121 1029
987 926 901 636 312 273 262 258
207 197 180 180 99 72 51 51
39 39 24
SnaBI TAC'GTA
Cuts at: 493 16137 493
Size: 15644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 18346
SphI G_CATG'C
Cuts at: 1132 6196 15704 1132
Size: 5064 9508 3774
Fragments arranged by size:
9508 5064 3774
SrfI GCCC'GGGC
Cuts at: 8387 8387
Size: 18346
SspI AAT'ATT
Cuts at: 5 52 16270 5
Size: 47 16218 2081
Fragments arranged by size:
16218 2081 47
StuI AGG'CCT
Cuts at: 1517 2669 3684 4353 4808 4997 5393 7625 11543
Size: 1152 1015 669 455 189 396 2232 3918
Cuts at: 11543 14474 14951 15068 1517
Size: 2931 477 117 4795
Fragments arranged by size:
4795 3918 2931 2232 1152 1015 669 477
455 396 189 117
StyI C'CwwG_G
Cuts at: 513 1065 2404 2484 2997 3021 3828 4368 5388
Size: 552 1339 80 513 24 807 540 1020
Cuts at: 5388 5658 5913 6309 7278 8052 8922 9579 10554
Size: 270 255 396 969 774 870 657 975
Cuts at: 10554 10671 11525 11538 12099 12531 12846 13683 13833
Size: 117 854 13 561 432 315 837 150
Cuts at: 13833 14116 14265 15417 15785 513
Size: 283 149 1152 368 3074
Fragments arranged by size:
3074 1339 1152 1020 975 969 870 854
837 807 774 657 561 552 540 513
432 396 368 315 283 270 255 150
149 117 80 24 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3586 4413 5603 8612 9290 11723 14261
Size: 1255 2006 827 1190 3009 678 2433 2538
Cuts at: 14261 16373 16390 16543 16728 17173 325
Size: 2112 17 153 185 445 1498
Fragments arranged by size:
3009 2538 2433 2112 2006 1498 1255 1190
827 678 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3465 4332 5826
Size: 274 80 33 51 1443 1488 867 1494
Cuts at: 5826 6000 11784 12675 16592 18304 96
Size: 174 5784 891 3917 1712 138
Fragments arranged by size:
5784 3917 1712 1494 1488 1443 891 867
274 174 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3095 3127 3878 3910 9294 9326 12734 12766 13009
Size: 32 751 32 5384 32 3408 32 243
Cuts at: 13009 13041 14519 14551 18306 18338 3095
Size: 32 1478 32 3755 32 3103
Fragments arranged by size:
5384 3755 3408 3103 1478 751 243 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 15008 1316
Size: 13692 4654
XbaI T'CTAG_A
Cuts at: 15682 15682
Size: 18346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3208 7366 9364 11953 12193 13690 14800 3208
Size: 4158 1998 2589 240 1497 1110 6754
Fragments arranged by size:
6754 4158 2589 1998 1497 1110 240
XhoI C'TCGA_G
Cuts at: 1943 5378 5609 12983 13034 1943
Size: 3435 231 7374 51 7255
Fragments arranged by size:
7374 7255 3435 231 51
XmaI C'CCGG_G
Cuts at: 1870 2271 3762 8292 8385 10707 11382 15495 15671
Size: 401 1491 4530 93 2322 675 4113 176
Cuts at: 15671 1870
Size: 4545
Fragments arranged by size:
4545 4530 4113 2322 1491 675 401 176
93
XmnI GAAnn'nnTTC
Cuts at: 2144 15068 16475 2144
Size: 12924 1407 4015
Fragments arranged by size:
12924 4015 1407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10714 14394 16154 276
Size: 53 83 186 936 9180 3680 1760 2468
Fragments arranged by size:
9180 3680 2468 1760 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I FspAI NruI NsiI
PacI RsrII SwaI