Restriction map for
pFN21AE2697
(Circular) (Six-base) MAPSORT of: pFN21AE2697.seq Check: 3147 from: 1 to: 6601
pFN21AE2697 6601 bp
With 182 enzymes: SgfI *
June 16, 2017 17:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4411 278
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3285 3424 3944 1937
Size: 1348 139 520 4594
Fragments arranged by size:
4594 1348 520 139
Acc65I G'GTAC_C
Cuts at: 3199 3922 3199
Size: 723 5878
AclI AA'CG_TT
Cuts at: 1563 4728 5101 1563
Size: 3165 373 3063
Fragments arranged by size:
3165 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2871 3456 3507 3722 4664 6048 6316 1632
Size: 1239 585 51 215 942 1384 268 1917
Fragments arranged by size:
1917 1384 1239 942 585 268 215 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6404
AflIII A'CryG_T
Cuts at: 1203 5500 6477 1203
Size: 4297 977 1327
Fragments arranged by size:
4297 1327 977
AgeI A'CCGG_T
Cuts at: 5432 5432
Size: 6601
AhdI GACnn_n'nnGTC
Cuts at: 5330 5330
Size: 6601
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2442 1184
Size: 1258 5343
AlwNI CAG_nnn'CTG
Cuts at: 3515 4344 5916 3515
Size: 829 1572 4200
Fragments arranged by size:
4200 1572 829
ApaLI G'TGCA_C
Cuts at: 4658 5814 4658
Size: 1156 5445
ApoI r'AATT_y
Cuts at: 1493 2843 3010 3910 4136 4288 6397 1493
Size: 1350 167 900 226 152 2109 1697
Fragments arranged by size:
2109 1697 1350 900 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 5155 160
Size: 872 4123 1606
Fragments arranged by size:
4123 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
AvaI C'yCGr_G
Cuts at: 1870 1943 2653 3926 1870
Size: 73 710 1273 4545
Fragments arranged by size:
4545 1273 710 73
BamHI G'GATC_C
Cuts at: 3731 3931 4298 3731
Size: 200 367 6034
Fragments arranged by size:
6034 367 200
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2468 3199 3922 5377 618
Size: 325 217 627 681 731 723 1455 1842
Fragments arranged by size:
1842 1455 731 723 681 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 3920 1391
Size: 645 1884 4072
Fragments arranged by size:
4072 1884 645
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2415 928
Size: 971 516 5114
Fragments arranged by size:
5114 971 516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4792 4826 1903
Size: 34 2855 34 3678
Fragments arranged by size:
3678 2855 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4493 5703 4493
Size: 1210 5391
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6601
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2122 2435 5212
Size: 107 122 71 631 605 450 313 2777
Cuts at: 5212 136
Size: 1525
Fragments arranged by size:
2777 1525 631 605 450 313 122 107
71
BglII A'GATC_T
Cuts at: 2342 2686 6596 2342
Size: 344 3910 2347
Fragments arranged by size:
3910 2347 344
BlpI GC'TnA_GC
Cuts at: 4018 4018
Size: 6601
Bme1580I G_kGCm'C
Cuts at: 1790 3447 4662 5818 1790
Size: 1657 1215 1156 2573
Fragments arranged by size:
2573 1657 1215 1156
BmgBI CAC'GTC
Cuts at: 1318 3641 1318
Size: 2323 4278
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4332 5290 442
Size: 416 53 83 399 24 2915 958 1753
Fragments arranged by size:
2915 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6601
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3548
Size: 6601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2651 3784 5261 1239
Size: 1412 1133 1477 2579
Fragments arranged by size:
2579 1477 1412 1133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3164 4726 5591 5889 3164
Size: 1562 865 298 3876
Fragments arranged by size:
3876 1562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5264 882
Size: 617 3765 2219
Fragments arranged by size:
3765 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3695 4392 493
Size: 711 2491 697 2702
Fragments arranged by size:
2702 2491 711 697
BsaBI GATnn'nnATC
Cuts at: 6595 6595
Size: 6601
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4408 4790 5425 275
Size: 53 83 186 936 2875 382 635 1451
Fragments arranged by size:
2875 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5034 5432 5706 5853 6264 5034
Size: 398 274 147 411 5371
Fragments arranged by size:
5371 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3656 3686 3885 3915 1178
Size: 30 165 30 2253 30 199 30 3864
Fragments arranged by size:
3864 2253 199 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 3603 3660 1186
Size: 838 1579 57 4127
Fragments arranged by size:
4127 1579 838 57
BseYI C'CCAG_C
Cuts at: 5804 6300 5804
Size: 496 6105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2767 1163
Size: 573 1031 4997
Fragments arranged by size:
4997 1031 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4051 4812 5840 1540
Size: 275 186 2050 761 1028 2301
Fragments arranged by size:
2301 2050 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2548 4662 4747 5818 2548
Size: 2114 85 1071 3331
Fragments arranged by size:
3331 2114 1071 85
BsmI GAATG_Cn'
Cuts at: 4112 4205 4112
Size: 93 6508
BsmBI CGTCTCn'nnnn_
Cuts at: 3519 3585 3519
Size: 66 6535
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2548 3447 3920 4662 4747 5818
Size: 399 246 512 899 473 742 85 1071
Cuts at: 5818 1391
Size: 2174
Fragments arranged by size:
2174 1071 899 742 512 473 399 246
85
BspEI T'CCGG_A
Cuts at: 6264 6264
Size: 6601
BspHI T'CATG_A
Cuts at: 2074 2137 2662 4490 6220 2074
Size: 63 525 1828 1730 2455
Fragments arranged by size:
2455 1828 1730 525 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2371 2789 3956 844
Size: 1527 418 1167 3489
Fragments arranged by size:
3489 1527 1167 418
BsrBI CCG'CTC
Cuts at: 1394 1823 4488 1394
Size: 429 2665 3507
Fragments arranged by size:
3507 2665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5096 5270 65
Size: 1183 3848 174 1396
Fragments arranged by size:
3848 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5245 5432 1700
Size: 110 3435 187 2869
Fragments arranged by size:
3435 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6601
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6601
BssSI C'ACGA_G
Cuts at: 3884 4661 5673 3884
Size: 777 1012 4812
Fragments arranged by size:
4812 1012 777
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2303 2468 3497 794
Size: 1509 165 1029 3898
Fragments arranged by size:
3898 1509 1029 165
BstBI TT'CG_AA
Cuts at: 5405 5405
Size: 6601
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6601
BstYI r'GATC_y
Cuts at: 1972 2342 2686 3731 3931 4298 4683 4700 6152
Size: 370 344 1045 200 367 385 17 1452
Cuts at: 6152 6238 6250 6596 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1045 385 370 367 346 344
200 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5110
Fragments arranged by size:
5110 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3217 487
Size: 683 56 1991 3871
Fragments arranged by size:
3871 1991 683 56
BtsI GCAGTG_nn'
Cuts at: 2754 2823 4117 4911 4931 2754
Size: 69 1294 794 20 4424
Fragments arranged by size:
4424 1294 794 69 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6566
DraI TTT'AAA
Cuts at: 2455 3276 3905 2455
Size: 821 629 5151
Fragments arranged by size:
5151 821 629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5608 809
Size: 507 4292 1802
Fragments arranged by size:
4292 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4048 4937 6566
Size: 54 1425 50 275 18 2218 889 1629
Cuts at: 6566 8
Size: 43
Fragments arranged by size:
2218 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4048 1537
Size: 275 2236 4090
Fragments arranged by size:
4090 2236 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2985 3558 3613 3628 4531 1353
Size: 1632 573 55 15 903 3423
Fragments arranged by size:
3423 1632 903 573 55 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5188 5562 5708 387
Size: 1064 138 3599 374 146 1280
Fragments arranged by size:
3599 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2651 2871 3456 3507 3722 3784 4664
Size: 393 1019 220 585 51 215 62 880
Cuts at: 4664 5261 6048 6316 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1019 880 787 597 585 393 268
220 215 62 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2160 2160
Size: 6601
EcoRI G'AATT_C
Cuts at: 3910 4288 3910
Size: 378 6223
FspI TGC'GCA
Cuts at: 2582 3584 3821 5107 2582
Size: 1002 237 1286 4076
Fragments arranged by size:
4076 1286 1002 237
HaeII r_GCGC'y
Cuts at: 1678 2046 5748 1678
Size: 368 3702 2531
Fragments arranged by size:
3702 2531 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3656 3688 5242 5274 5316
Size: 32 135 32 2084 32 1554 32 42
Cuts at: 5316 5348 1373
Size: 32 2626
Fragments arranged by size:
2626 2084 1554 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3751 3945 4191 669
Size: 988 281 1813 194 246 3079
Fragments arranged by size:
3079 1813 988 281 246 194
HindIII A'AGCT_T
Cuts at: 748 2833 748
Size: 2085 4516
HpaI GTT'AAC
Cuts at: 4191 4191
Size: 6601
KpnI G_GTAC'C
Cuts at: 3203 3926 3203
Size: 723 5878
MfeI C'AATT_G
Cuts at: 2328 4200 2328
Size: 1872 4729
MluI A'CGCG_T
Cuts at: 6477 6477
Size: 6601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3019 3578 5715 5899 1305
Size: 1714 559 2137 184 2007
Fragments arranged by size:
2137 2007 1714 559 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4779
Fragments arranged by size:
4779 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2796 4559 4918 5077 518
Size: 648 1630 1763 359 159 2042
Fragments arranged by size:
2042 1763 1630 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2629 4017 4694 5842 6087 1606
Size: 1023 1388 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 1023 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5110
Fragments arranged by size:
5110 939 552
NdeI CA'TA_TG
Cuts at: 387 2550 387
Size: 2163 4438
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6601
NotI GC'GGCC_GC
Cuts at: 4048 4048
Size: 6601
NspI r_CATG'y
Cuts at: 1132 1229 2301 2566 3959 5504 1132
Size: 97 1072 265 1393 1545 2229
Fragments arranged by size:
2229 1545 1393 1072 265 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6601
PciI A'CATG_T
Cuts at: 5500 5500
Size: 6601
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6601
PfoI T'CCnGG_A
Cuts at: 3460 3460
Size: 6601
PmeI GTTT'AAAC
Cuts at: 3905 3905
Size: 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 6183 6215 4670
Size: 32 1481 32 5056
Fragments arranged by size:
5056 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6601
PsiI TTA'TAA
Cuts at: 2169 4171 2169
Size: 2002 4599
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2055 2087 2850 2882 2055
Size: 32 763 32 5774
Fragments arranged by size:
5774 763 32 32
PstI C_TGCA'G
Cuts at: 830 3953 830
Size: 3123 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6601
PvuII CAG'CTG
Cuts at: 2629 2629
Size: 6601
SalI G'TCGA_C
Cuts at: 1936 3943 1936
Size: 2007 4594
SbfI CC_TGCA'GG
Cuts at: 3953 3953
Size: 6601
ScaI AGT'ACT
Cuts at: 4849 6561 4849
Size: 1712 4889
SfcI C'TryA_G
Cuts at: 826 1045 2160 3949 5084 5765 5956 826
Size: 219 1115 1789 1135 681 191 1471
Fragments arranged by size:
1789 1471 1135 1115 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6601
SmaI CCC'GGG
Cuts at: 1872 3928 1872
Size: 2056 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2653 3179 4705 5606 5868 820
Size: 197 926 710 526 1526 901 262 1553
Fragments arranged by size:
1553 1526 926 901 710 526 262 197
SnaBI TAC'GTA
Cuts at: 493 4392 493
Size: 3899 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6601
SphI G_CATG'C
Cuts at: 1132 3959 1132
Size: 2827 3774
SspI AAT'ATT
Cuts at: 5 52 2725 4525 5
Size: 47 2673 1800 2081
Fragments arranged by size:
2673 2081 1800 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6601
StyI C'CwwG_G
Cuts at: 513 1065 2004 2870 4040 513
Size: 552 939 866 1170 3074
Fragments arranged by size:
3074 1170 939 866 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3107 4628 4645 4798 4983 5428 325
Size: 1255 1527 1521 17 153 185 445 1498
Fragments arranged by size:
1527 1521 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3113 4847 6559
Size: 274 80 33 51 1443 1136 1734 1712
Cuts at: 6559 96
Size: 138
Fragments arranged by size:
1734 1712 1443 1136 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6561 6593 6561
Size: 32 6569
Tth111I GACn'n_nGTC
Cuts at: 1316 3456 1316
Size: 2140 4461
XbaI T'CTAG_A
Cuts at: 2767 3347 3937 2767
Size: 580 590 5431
Fragments arranged by size:
5431 590 580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3250 2011
Size: 1239 5362
XhoI C'TCGA_G
Cuts at: 1943 2653 1943
Size: 710 5891
XmaI C'CCGG_G
Cuts at: 1870 3926 1870
Size: 2056 4545
XmnI GAAnn'nnTTC
Cuts at: 3481 4730 3481
Size: 1249 5352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4409 276
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI