Restriction map for
pFN21AE2696
(Circular) (Six-base) MAPSORT of: pFN21AE2696.seq Check: 3242 from: 1 to: 5626
pFN21AE2696 5626 bp
With 182 enzymes: SgfI *
June 29, 2017 18:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2127 3436 278
Size: 53 83 186 936 591 1309 2468
Fragments arranged by size:
2468 1309 936 591 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2403 2969 1937
Size: 466 566 4594
Fragments arranged by size:
4594 566 466
Acc65I G'GTAC_C
Cuts at: 2310 2947 2310
Size: 637 4989
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3689 5073 5341 1632
Size: 2057 1384 268 1917
Fragments arranged by size:
2057 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 4525 5502 1203
Size: 3322 977 1327
Fragments arranged by size:
3322 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 2480 1166
Size: 1314 4312
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2745 1184
Size: 1561 4065
AlwNI CAG_nnn'CTG
Cuts at: 3369 4941 3369
Size: 1572 4054
ApaLI G'TGCA_C
Cuts at: 2597 3683 4839 2597
Size: 1086 1156 3384
Fragments arranged by size:
3384 1156 1086
ApoI r'AATT_y
Cuts at: 1493 2935 3161 3313 5422 1493
Size: 1442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 1870
Size: 73 1008 4545
Fragments arranged by size:
4545 1008 73
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2310 2947 4402 618
Size: 325 217 627 523 637 1455 1842
Fragments arranged by size:
1842 1455 637 627 523 325 217
BanII G_rGCy'C
Cuts at: 1391 2945 1391
Size: 1554 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2366 928
Size: 971 467 4188
Fragments arranged by size:
4188 971 467
BbvCI CC'TCA_GC
Cuts at: 2315 2315
Size: 5626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3817 3851 1903
Size: 34 1880 34 3678
Fragments arranged by size:
3678 1880 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4237 136
Size: 107 122 71 631 605 2565 1525
Fragments arranged by size:
2565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 2225 3043 2225
Size: 818 4808
Bme1580I G_kGCm'C
Cuts at: 1790 2601 3687 4843 1790
Size: 811 1086 1156 2573
Fragments arranged by size:
2573 1156 1086 811
BmgBI CAC'GTC
Cuts at: 1318 2242 2379 1318
Size: 924 137 4565
Fragments arranged by size:
4565 924 137
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2287 3357 4315
Size: 416 53 83 399 24 870 1070 958
Cuts at: 4315 442
Size: 1753
Fragments arranged by size:
1753 1070 958 870 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2237 4286 1239
Size: 860 138 2049 2579
Fragments arranged by size:
2579 2049 860 138
Bpu10I CC'TnA_GC
Cuts at: 2315 2394 2315
Size: 79 5547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3751 4616 4914 3751
Size: 865 298 4463
Fragments arranged by size:
4463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2439 4289 882
Size: 617 940 1850 2219
Fragments arranged by size:
2219 1850 940 617
BsaAI yAC'GTr
Cuts at: 493 1204 2596 3417 493
Size: 711 1392 821 2702
Fragments arranged by size:
2702 1392 821 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 3433 3815 4450
Size: 53 83 186 936 591 1309 382 635
Cuts at: 4450 275
Size: 1451
Fragments arranged by size:
1451 1309 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5401
Fragments arranged by size:
5401 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2543 1186
Size: 1111 246 4269
Fragments arranged by size:
4269 1111 246
BseYI C'CCAG_C
Cuts at: 2043 2049 2157 4829 5325 2043
Size: 6 108 2672 496 2344
Fragments arranged by size:
2672 2344 496 108 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 2843 1163
Size: 573 480 627 3946
Fragments arranged by size:
3946 627 573 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2250 2659 2788 3076 3837 4865
Size: 275 186 249 409 129 288 761 1028
Cuts at: 4865 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 409 288 275 249 186
129
BsiHKAI G_wGCw'C
Cuts at: 2601 3687 3772 4843 2601
Size: 1086 85 1071 3384
Fragments arranged by size:
3384 1086 1071 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2601 2945 3687 3772 4843 1391
Size: 399 811 344 742 85 1071 2174
Fragments arranged by size:
2174 1071 811 742 399 344 85
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 2872 3515 5245 2872
Size: 643 1730 3253
Fragments arranged by size:
3253 1730 643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 844
Size: 2137 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4121 4295 65
Size: 1183 2873 174 1396
Fragments arranged by size:
2873 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4270 4457 1700
Size: 110 2460 187 2869
Fragments arranged by size:
2869 2460 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 2206 1925
Size: 281 5345
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5626
BstBI TT'CG_AA
Cuts at: 2735 4430 2735
Size: 1695 3931
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2685 2956 3323 3708 3725 5177 5263 5275
Size: 713 271 367 385 17 1452 86 12
Cuts at: 5275 5621 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 713 385 367 346 271 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2250 2296 513
Size: 552 939 246 46 3843
Fragments arranged by size:
3843 939 552 246 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2145 2559 487
Size: 683 56 919 414 3554
Fragments arranged by size:
3554 919 683 414 56
BtsI GCAGTG_nn'
Cuts at: 2210 3142 3936 3956 2210
Size: 932 794 20 3880
Fragments arranged by size:
3880 932 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DraIII CAC_nnn'GTG
Cuts at: 2647 2647
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2247 2336
Size: 54 1425 50 275 18 177 240 89
Cuts at: 2336 2785 3073 3962 5591 8
Size: 449 288 889 1629 43
Fragments arranged by size:
1629 1425 889 449 288 275 240 177
89 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2247 2785 3073 1537
Size: 275 435 538 288 4090
Fragments arranged by size:
4090 538 435 288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3556 1353
Size: 2203 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2237 3689 4286 5073 5341 1239
Size: 393 467 138 1452 597 787 268 1524
Fragments arranged by size:
1524 1452 787 597 467 393 268 138
EcoO109I rG'GnC_Cy
Cuts at: 2109 2572 2671 2109
Size: 463 99 5064
Fragments arranged by size:
5064 463 99
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 5626
FspI TGC'GCA
Cuts at: 2192 4132 2192
Size: 1940 3686
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373
Size: 32 135 32 2695 32 42 32 2626
Fragments arranged by size:
2695 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2314 2951 2314
Size: 637 4989
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 4740 4924 1305
Size: 1128 2307 184 2007
Fragments arranged by size:
2307 2007 1128 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2338 10
Size: 54 1425 343 506 3298
Fragments arranged by size:
3298 1425 506 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2480 3584 3943 4102 518
Size: 648 1020 294 1104 359 159 2042
Fragments arranged by size:
2042 1104 1020 648 359 294 159
MspA1I CmG'CkG
Cuts at: 1606 2252 2629 2784 2796 3042 3719 4867 5112
Size: 646 377 155 12 246 677 1148 245
Cuts at: 5112 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 646 377 246 245 155
12
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4135
Fragments arranged by size:
4135 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 2247 3073 2247
Size: 826 4800
NspI r_CATG'y
Cuts at: 1132 1229 2500 2605 2984 4529 1132
Size: 97 1271 105 379 1545 2229
Fragments arranged by size:
2229 1545 1271 379 105 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 2647 1108
Size: 1194 345 4087
Fragments arranged by size:
4087 1194 345
PfoI T'CCnGG_A
Cuts at: 2115 2115
Size: 5626
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PmlI CAC'GTG
Cuts at: 2596 2596
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2109 2671 2109
Size: 562 5064
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2978 830
Size: 2148 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
SacII CC_GC'GG
Cuts at: 2253 2253
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SanDI GG'GwC_CC
Cuts at: 2109 2671 2109
Size: 562 5064
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2404 2974 4109 4790 4981 826
Size: 219 1359 570 1135 681 191 1471
Fragments arranged by size:
1471 1359 1135 681 570 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3730 4631 4893 820
Size: 197 926 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 2867 3550 5
Size: 47 2815 683 2081
Fragments arranged by size:
2815 2081 683 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 2004 2757 3065 513
Size: 552 939 753 308 3074
Fragments arranged by size:
3074 939 753 552 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2492 3653 3670 3823 4008 4453 325
Size: 1255 912 1161 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1161 912 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3872 5584 96
Size: 274 80 33 51 1443 1895 1712 138
Fragments arranged by size:
1895 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 2868 2891 3755 2868
Size: 23 864 4739
Fragments arranged by size:
4739 864 23
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2125 3434 276
Size: 53 83 186 936 591 1309 2468
Fragments arranged by size:
2468 1309 936 591 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII
SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI