Restriction map for
pFN21AE2694
(Circular) (Six-base) MAPSORT of: pFN21AE2694.seq Check: 3146 from: 1 to: 8452
pFN21AE2694 8452 bp
With 182 enzymes: SgfI *
June 29, 2017 18:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2312 2419 3587 2312
Size: 107 1168 7177
Fragments arranged by size:
7177 1168 107
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2691 6262 278
Size: 53 83 186 936 1155 3571 2468
Fragments arranged by size:
3571 2468 1155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2802 5154 5795 1937
Size: 865 2352 641 4594
Fragments arranged by size:
4594 2352 865 641
Acc65I G'GTAC_C
Cuts at: 5773 5773
Size: 8452
AclI AA'CG_TT
Cuts at: 1563 6579 6952 1563
Size: 5016 373 3063
Fragments arranged by size:
5016 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 2521 3959 4382 4556 4796 5357 6515
Size: 434 455 1438 423 174 240 561 1158
Cuts at: 6515 7899 8167 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1438 1384 1158 561 455 434 423
268 240 174
AfeI AGC'GCT
Cuts at: 2043 2043
Size: 8452
AflII C'TTAA_G
Cuts at: 820 1017 4031 820
Size: 197 3014 5241
Fragments arranged by size:
5241 3014 197
AflIII A'CryG_T
Cuts at: 1203 4728 7351 8328 1203
Size: 3525 2623 977 1327
Fragments arranged by size:
3525 2623 1327 977
AgeI A'CCGG_T
Cuts at: 4325 7283 4325
Size: 2958 5494
AhdI GACnn_n'nnGTC
Cuts at: 7181 7181
Size: 8452
AleI CACnn'nnGTG
Cuts at: 1166 3857 4326 5478 1166
Size: 2691 469 1152 4140
Fragments arranged by size:
4140 2691 1152 469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2141 2669 1184
Size: 957 528 6967
Fragments arranged by size:
6967 957 528
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 4715 4747 2543
Size: 32 2140 32 6248
Fragments arranged by size:
6248 2140 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2209 2398 2683 2878 3176 3179 3718 4714 4834
Size: 189 285 195 298 3 539 996 120
Cuts at: 4834 5042 5335 6195 7767 2209
Size: 208 293 860 1572 2894
Fragments arranged by size:
2894 1572 996 860 539 298 293 285
208 195 189 120 3
ApaI G_GGCC'C
Cuts at: 2627 3545 4896 5635 2627
Size: 918 1351 739 5444
Fragments arranged by size:
5444 1351 918 739
ApaLI G'TGCA_C
Cuts at: 6509 7665 6509
Size: 1156 7296
ApoI r'AATT_y
Cuts at: 1493 5761 5987 6139 8248 1493
Size: 4268 226 152 2109 1697
Fragments arranged by size:
4268 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7006 160
Size: 872 5974 1606
Fragments arranged by size:
5974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8452
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 2468 2627 3487 3867 4043 4461
Size: 73 165 360 159 860 380 176 418
Cuts at: 4461 4860 4895 4974 5136 5608 5634 5777 1870
Size: 399 35 79 162 472 26 143 4545
Fragments arranged by size:
4545 860 472 418 399 380 360 176
165 162 159 143 79 73 35 26
BamHI G'GATC_C
Cuts at: 5020 5272 5401 5782 6149 5020
Size: 252 129 381 367 7323
Fragments arranged by size:
7323 381 367 252 129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5483 5773 7228 618
Size: 325 217 627 3696 290 1455 1842
Fragments arranged by size:
3696 1842 1455 627 325 290 217
BanII G_rGCy'C
Cuts at: 1391 2559 2627 2913 3059 3192 3244 3315 3545
Size: 1168 68 286 146 133 52 71 230
Cuts at: 3545 3647 3795 3852 3885 4896 5106 5635 5771
Size: 102 148 57 33 1011 210 529 136
Cuts at: 5771 1391
Size: 4072
Fragments arranged by size:
4072 1168 1011 529 286 230 210 148
146 136 133 102 71 68 57 52
33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4973 928
Size: 971 3074 4407
Fragments arranged by size:
4407 3074 971
BbvCI CC'TCA_GC
Cuts at: 2360 5175 2360
Size: 2815 5637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4143 4177 6643 6677 1903
Size: 34 2206 34 2466 34 3678
Fragments arranged by size:
3678 2466 2206 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6344 7554 6344
Size: 1210 7242
BclI T'GATC_A
Cuts at: 1548 2604 1548
Size: 1056 7396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2178 3111 3869
Size: 107 122 71 631 605 506 933 758
Cuts at: 3869 4097 4577 4634 4934 7063 136
Size: 228 480 57 300 2129 1525
Fragments arranged by size:
2129 1525 933 758 631 605 506 480
300 228 122 107 71 57
BglII A'GATC_T
Cuts at: 8447 8447
Size: 8452
BlpI GC'TnA_GC
Cuts at: 2597 3646 5869 2597
Size: 1049 2223 5180
Fragments arranged by size:
5180 2223 1049
Bme1580I G_kGCm'C
Cuts at: 1790 2580 2627 3545 4639 4896 5285 5621 5635
Size: 790 47 918 1094 257 389 336 14
Cuts at: 5635 6513 7669 1790
Size: 878 1156 2573
Fragments arranged by size:
2573 1156 1094 918 878 790 389 336
257 47 14
BmgBI CAC'GTC
Cuts at: 1318 5409 1318
Size: 4091 4361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3339 5272 6183
Size: 416 53 83 399 24 1922 1933 911
Cuts at: 6183 7141 442
Size: 958 1753
Fragments arranged by size:
1933 1922 1753 958 911 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8452
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3763 4543 3763
Size: 780 7672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2195 2231 2258 2732 2759 2908 2927
Size: 875 81 36 27 474 27 149 19
Cuts at: 2927 2969 3026 3266 3787 4008 4439 4523 4568
Size: 42 57 240 521 221 431 84 45
Cuts at: 4568 4595 4742 5207 5240 5338 5411 5480 5668
Size: 27 147 465 33 98 73 69 188
Cuts at: 5668 7112 1239
Size: 1444 2579
Fragments arranged by size:
2579 1444 875 521 474 465 431 240
221 188 149 147 98 84 81 73
69 57 45 42 36 33 27 27
27 19
Bpu10I CC'TnA_GC
Cuts at: 2360 2757 3357 3764 5175 5328 2360
Size: 397 600 407 1411 153 5484
Fragments arranged by size:
5484 1411 600 407 397 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5330 6577 7442 7740 5330
Size: 1247 865 298 6042
Fragments arranged by size:
6042 1247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2700 4367 4544 7115 882
Size: 617 1201 1667 177 2571 2219
Fragments arranged by size:
2571 2219 1667 1201 617 177
BsaAI yAC'GTr
Cuts at: 493 1204 2276 2377 2479 2584 3737 4414 6243
Size: 711 1072 101 102 105 1153 677 1829
Cuts at: 6243 493
Size: 2702
Fragments arranged by size:
2702 1829 1153 1072 711 677 105 102
101
BsaBI GATnn'nnATC
Cuts at: 8446 8446
Size: 8452
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2286 2493 2688 3257
Size: 53 83 186 936 753 207 195 569
Cuts at: 3257 3758 3777 4332 4704 5157 5421 6259 6641
Size: 501 19 555 372 453 264 838 382
Cuts at: 6641 7276 275
Size: 635 1451
Fragments arranged by size:
1451 936 838 753 635 569 555 501
453 382 372 264 207 195 186 83
53 19
BsaWI w'CCGG_w
Cuts at: 4325 4791 6885 7283 7557 7704 8115 4325
Size: 466 2094 398 274 147 411 4662
Fragments arranged by size:
4662 2094 466 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 3276 3306 4364
Size: 30 165 30 1140 30 703 30 1058
Cuts at: 4364 4394 4715 4745 4898 4928 5058 5088 1178
Size: 30 321 30 153 30 130 30 4542
Fragments arranged by size:
4542 1140 1058 703 321 165 153 130
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2765 2786 2789 3417 3537 3623 3977 4097
Size: 1579 21 3 628 120 86 354 120
Cuts at: 4097 4112 4834 4856 4859 4922 5213 5546 5596
Size: 15 722 22 3 63 291 333 50
Cuts at: 5596 1186
Size: 4042
Fragments arranged by size:
4042 1579 722 628 354 333 291 120
120 86 63 50 22 21 15 3
3
BseYI C'CCAG_C
Cuts at: 2056 2649 2871 3146 3418 3537 3659 4218 4383
Size: 593 222 275 272 119 122 559 165
Cuts at: 4383 4707 5142 5286 5416 7655 8151 2056
Size: 324 435 144 130 2239 496 2357
Fragments arranged by size:
2357 2239 593 559 496 435 324 275
272 222 165 144 130 122 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 2539 2885 3559 4391 1163
Size: 573 486 317 346 674 832 5224
Fragments arranged by size:
5224 832 674 573 486 346 317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4188 5902 6663 7691 1540
Size: 275 186 2187 1714 761 1028 2301
Fragments arranged by size:
2301 2187 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2559 2976 3744 3956 6513 6598 7669 2559
Size: 417 768 212 2557 85 1071 3342
Fragments arranged by size:
3342 2557 1071 768 417 212 85
BsmI GAATG_Cn'
Cuts at: 3002 4073 5963 6056 3002
Size: 1071 1890 93 5398
Fragments arranged by size:
5398 1890 1071 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2559 2580 2627 2913 2976 3059 3192
Size: 399 769 21 47 286 63 83 133
Cuts at: 3192 3244 3315 3545 3647 3744 3795 3852 3885
Size: 52 71 230 102 97 51 57 33
Cuts at: 3885 3956 4639 4896 5106 5285 5621 5635 5771
Size: 71 683 257 210 179 336 14 136
Cuts at: 5771 6513 6598 7669 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 769 742 683 399 336 286
257 230 210 179 136 133 102 97
85 83 71 71 63 57 52 51
47 33 21 14
BspEI T'CCGG_A
Cuts at: 8115 8115
Size: 8452
BspHI T'CATG_A
Cuts at: 2328 2547 6341 8071 2328
Size: 219 3794 1730 2709
Fragments arranged by size:
3794 2709 1730 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 2312 2419 2643 2733 3587 4074 5807
Size: 1394 74 107 224 90 854 487 1733
Cuts at: 5807 844
Size: 3489
Fragments arranged by size:
3489 1733 1394 854 487 224 107 90
74
BsrBI CCG'CTC
Cuts at: 1394 1823 2220 2922 3022 4515 4558 6339 1394
Size: 429 397 702 100 1493 43 1781 3507
Fragments arranged by size:
3507 1781 1493 702 429 397 100 43
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 6947 7121 65
Size: 1183 3107 2592 174 1396
Fragments arranged by size:
3107 2592 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4325 5308 7096 7283 1700
Size: 110 2515 983 1788 187 2869
Fragments arranged by size:
2869 2515 1788 983 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8452
BssHII G'CGCG_C
Cuts at: 1925 2320 2638 2640 2642 1925
Size: 395 318 2 2 7735
Fragments arranged by size:
7735 395 318 2 2
BssSI C'ACGA_G
Cuts at: 2957 4052 6512 7524 2957
Size: 1095 2460 1012 3885
Fragments arranged by size:
3885 2460 1095 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3176 3179 3718 4414 794
Size: 2382 3 539 696 4832
Fragments arranged by size:
4832 2382 696 539 3
BstBI TT'CG_AA
Cuts at: 7256 7256
Size: 8452
BstEII G'GTnAC_C
Cuts at: 1184 2587 5596 1184
Size: 1403 3009 4040
Fragments arranged by size:
4040 3009 1403
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 3244 3859 5480 2416
Size: 828 615 1621 5388
Fragments arranged by size:
5388 1621 828 615
BstYI r'GATC_y
Cuts at: 1972 5020 5068 5272 5401 5782 6149 6534 6551
Size: 3048 48 204 129 381 367 385 17
Cuts at: 6551 8003 8089 8101 8447 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3048 1977 1452 385 381 367 346 204
129 86 48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2079 2263 3192 3408 3591 3634 3855
Size: 552 1014 184 929 216 183 43 221
Cuts at: 3855 4575 4981 5016 5073 5210 5479 513
Size: 720 406 35 57 137 269 3486
Fragments arranged by size:
3486 1014 929 720 552 406 269 221
216 184 183 137 57 43 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3000 5268 487
Size: 683 56 1774 2268 3671
Fragments arranged by size:
3671 2268 1774 683 56
BtsI GCAGTG_nn'
Cuts at: 3686 5968 6762 6782 3686
Size: 2282 794 20 5356
Fragments arranged by size:
5356 2282 794 20
ClaI AT'CG_AT
Cuts at: 1554 4923 1554
Size: 3369 5083
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3985 4020 529
Size: 35 3421 35 4961
Fragments arranged by size:
4961 3421 35 35
DraI TTT'AAA
Cuts at: 5756 5756
Size: 8452
DraIII CAC_nnn'GTG
Cuts at: 2848 4414 2848
Size: 1566 6886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7459 809
Size: 507 6143 1802
Fragments arranged by size:
6143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2979 4098 4131
Size: 54 1425 50 275 18 1149 1119 33
Cuts at: 4131 4185 4578 4683 5046 5306 5899 6788 8417
Size: 54 393 105 363 260 593 889 1629
Cuts at: 8417 8
Size: 43
Fragments arranged by size:
1629 1425 1149 1119 889 593 393 363
275 260 105 54 54 50 43 33
18
EagI C'GGCC_G
Cuts at: 1537 1812 4185 5899 1537
Size: 275 2373 1714 4090
Fragments arranged by size:
4090 2373 1714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3908 5691 6382 1353
Size: 2555 1783 691 3423
Fragments arranged by size:
3423 2555 1783 691
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4853 7039 7413 7559 387
Size: 1064 138 3264 2186 374 146 1280
Fragments arranged by size:
3264 2186 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2557 2557
Size: 8452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2114 2195 2231 2258 2521 2732
Size: 393 434 48 81 36 27 263 211
Cuts at: 2732 2759 2908 2927 2969 3026 3266 3787 3959
Size: 27 149 19 42 57 240 521 172
Cuts at: 3959 4008 4382 4439 4523 4556 4568 4595 4742
Size: 49 374 57 84 33 12 27 147
Cuts at: 4742 4796 5207 5240 5338 5357 5411 5480 5668
Size: 54 411 33 98 19 54 69 188
Cuts at: 5668 6515 7112 7899 8167 1239
Size: 847 597 787 268 1524
Fragments arranged by size:
1524 847 787 597 521 434 411 393
374 268 263 240 211 188 172 149
147 98 84 81 69 57 57 54
54 49 48 42 36 33 33 27
27 27 19 19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3065 4697 5499 3065
Size: 1632 802 6018
Fragments arranged by size:
6018 1632 802
EcoO109I rG'GnC_Cy
Cuts at: 2395 2624 2632 2893 2944 3361 3541 3542 3655
Size: 229 8 261 51 417 180 1 113
Cuts at: 3655 4465 4756 4892 5146 5301 5332 5631 2395
Size: 810 291 136 254 155 31 299 5216
Fragments arranged by size:
5216 810 417 299 291 261 254 229
180 155 136 113 51 31 8 1
EcoRI G'AATT_C
Cuts at: 5761 6139 5761
Size: 378 8074
FspI TGC'GCA
Cuts at: 2165 3692 6958 2165
Size: 1527 3266 3659
Fragments arranged by size:
3659 3266 1527
HaeII r_GCGC'y
Cuts at: 1678 2045 2173 2246 4107 4609 7599 1678
Size: 367 128 73 1861 502 2990 2531
Fragments arranged by size:
2990 2531 1861 502 367 128 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4364 4396 5393 5425 7093
Size: 32 135 32 2792 32 997 32 1668
Cuts at: 7093 7125 7167 7199 1373
Size: 32 42 32 2626
Fragments arranged by size:
2792 2626 1668 997 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2149 2803 5796 6042 669
Size: 988 281 211 654 2993 246 3079
Fragments arranged by size:
3079 2993 988 654 281 246 211
HindIII A'AGCT_T
Cuts at: 748 4541 748
Size: 3793 4659
HpaI GTT'AAC
Cuts at: 6042 6042
Size: 8452
KpnI G_GTAC'C
Cuts at: 5777 5777
Size: 8452
MfeI C'AATT_G
Cuts at: 6051 6051
Size: 8452
MluI A'CGCG_T
Cuts at: 8328 8328
Size: 8452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2307 2433 3750 3981 4554 5175 7566 7750
Size: 1002 126 1317 231 573 621 2391 184
Cuts at: 7750 1305
Size: 2007
Fragments arranged by size:
2391 2007 1317 1002 621 573 231 184
126
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2981 4100 4133 5048 10
Size: 54 1425 343 1149 1119 33 915 3414
Fragments arranged by size:
3414 1425 1149 1119 915 343 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2546 3857 4326 5478 6410 6769
Size: 648 843 537 1311 469 1152 932 359
Cuts at: 6769 6928 518
Size: 159 2042
Fragments arranged by size:
2042 1311 1152 932 843 648 537 469
359 159
MspA1I CmG'CkG
Cuts at: 1606 2265 2313 2653 2680 2737 2932 3176 4150
Size: 659 48 340 27 57 195 244 974
Cuts at: 4150 4222 4264 4282 4456 4714 4834 4960 4983
Size: 72 42 18 174 258 120 126 23
Cuts at: 4983 5868 6545 7693 7938 1606
Size: 885 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 885 677 659 340 258
245 244 195 174 126 120 72 57
48 42 27 23 18
NcoI C'CATG_G
Cuts at: 513 1065 3408 3634 3855 5016 513
Size: 552 2343 226 221 1161 3949
Fragments arranged by size:
3949 2343 1161 552 226 221
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8452
NotI GC'GGCC_GC
Cuts at: 4185 5899 4185
Size: 1714 6738
NspI r_CATG'y
Cuts at: 1132 1229 4168 5810 7355 1132
Size: 97 2939 1642 1545 2229
Fragments arranged by size:
2939 2229 1642 1545 97
PasI CC'CwG_GG
Cuts at: 1348 5628 1348
Size: 4280 4172
PciI A'CATG_T
Cuts at: 7351 7351
Size: 8452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 3640 4417 5293 1108
Size: 1290 1242 777 876 4267
Fragments arranged by size:
4267 1290 1242 876 777
PfoI T'CCnGG_A
Cuts at: 2208 4587 4752 5684 2208
Size: 2379 165 932 4976
Fragments arranged by size:
4976 2379 932 165
PmeI GTTT'AAAC
Cuts at: 5756 5756
Size: 8452
PmlI CAC'GTG
Cuts at: 2479 3737 4414 2479
Size: 1258 677 6517
Fragments arranged by size:
6517 1258 677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2575 4715 4747 6521 6553 8034 8066 2543
Size: 32 2140 32 1774 32 1481 32 2929
Fragments arranged by size:
2929 2140 1774 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 3655 4465 4756 5146 2632
Size: 1023 810 291 390 5938
Fragments arranged by size:
5938 1023 810 390 291
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8452
PsiI TTA'TAA
Cuts at: 6022 6022
Size: 8452
PspOMI G'GGCC_C
Cuts at: 2623 3541 4892 5631 2623
Size: 918 1351 739 5444
Fragments arranged by size:
5444 1351 918 739
PstI C_TGCA'G
Cuts at: 830 2298 2679 2892 3584 3835 4455 5065 5374
Size: 1468 381 213 692 251 620 610 309
Cuts at: 5374 5500 5804 830
Size: 126 304 3478
Fragments arranged by size:
3478 1468 692 620 610 381 309 304
251 213 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8452
PvuII CAG'CTG
Cuts at: 2313 2653 2680 2737 2932 3176 4150 4222 4264
Size: 340 27 57 195 244 974 72 42
Cuts at: 4264 4282 4456 4714 4834 4960 2313
Size: 18 174 258 120 126 5805
Fragments arranged by size:
5805 974 340 258 244 195 174 126
120 72 57 42 27 18
SacI G_AGCT'C
Cuts at: 2559 2559
Size: 8452
SacII CC_GC'GG
Cuts at: 2266 4984 2266
Size: 2718 5734
SalI G'TCGA_C
Cuts at: 1936 2801 5794 1936
Size: 865 2993 4594
Fragments arranged by size:
4594 2993 865
SanDI GG'GwC_CC
Cuts at: 3655 3655
Size: 8452
SapI GCTCTTCn'nnn_
Cuts at: 3908 3908
Size: 8452
SbfI CC_TGCA'GG
Cuts at: 5065 5804 5065
Size: 739 7713
ScaI AGT'ACT
Cuts at: 6700 8412 6700
Size: 1712 6740
SfcI C'TryA_G
Cuts at: 826 1045 2294 2675 2888 3580 3831 4451 5061
Size: 219 1249 381 213 692 251 620 610
Cuts at: 5061 5370 5496 5800 6935 7616 7807 826
Size: 309 126 304 1135 681 191 1471
Fragments arranged by size:
1471 1249 1135 692 681 620 610 381
309 304 251 219 213 191 126
SfiI GGCCn_nnn'nGGCC
Cuts at: 3869 3869
Size: 8452
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8452
SgrAI Cr'CCGG_yG
Cuts at: 1700 4325 1700
Size: 2625 5827
SmaI CCC'GGG
Cuts at: 1872 3489 3869 4463 4897 4976 5138 5636 5779
Size: 1617 380 594 434 79 162 498 143
Cuts at: 5779 1872
Size: 4545
Fragments arranged by size:
4545 1617 594 498 434 380 162 143
79
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4031 5345 6556 7457 7719 820
Size: 197 926 2088 1314 1211 901 262 1553
Fragments arranged by size:
2088 1553 1314 1211 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6243 493
Size: 5750 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8452
SphI G_CATG'C
Cuts at: 1132 4168 5810 1132
Size: 3036 1642 3774
Fragments arranged by size:
3774 3036 1642
SspI AAT'ATT
Cuts at: 5 52 6376 5
Size: 47 6324 2081
Fragments arranged by size:
6324 2081 47
StuI AGG'CCT
Cuts at: 1517 3399 1517
Size: 1882 6570
StyI C'CwwG_G
Cuts at: 513 1065 3408 3634 3843 3855 3885 4212 5016
Size: 552 2343 226 209 12 30 327 804
Cuts at: 5016 5292 5397 5891 513
Size: 276 105 494 3074
Fragments arranged by size:
3074 2343 804 552 494 327 276 226
209 105 30 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2663 3067 3697 4568 5615 6479 6496
Size: 1255 1083 404 630 871 1047 864 17
Cuts at: 6496 6649 6834 7279 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1083 1047 871 864 630 445
404 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 4596 6698
Size: 274 80 33 51 1443 375 2244 2102
Cuts at: 6698 8410 96
Size: 1712 138
Fragments arranged by size:
2244 2102 1712 1443 375 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3276 3308 4898 4930 5058 5074 5090 5106 5257
Size: 32 1590 32 128 16 16 16 151
Cuts at: 5257 5264 5289 5296 8412 8444 3276
Size: 7 25 7 3116 32 3284
Fragments arranged by size:
3284 3116 1590 151 128 32 32 32
25 16 16 16 7 7
Tth111I GACn'n_nGTC
Cuts at: 1316 4378 5468 1316
Size: 3062 1090 4300
Fragments arranged by size:
4300 3062 1090
XbaI T'CTAG_A
Cuts at: 5788 5788
Size: 8452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3148 3632 4006 4219 5041 5413 3148
Size: 484 374 213 822 372 6187
Fragments arranged by size:
6187 822 484 374 372 213
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8452
XmaI C'CCGG_G
Cuts at: 1870 3487 3867 4461 4895 4974 5136 5634 5777
Size: 1617 380 594 434 79 162 498 143
Cuts at: 5777 1870
Size: 4545
Fragments arranged by size:
4545 1617 594 498 434 380 162 143
79
XmnI GAAnn'nnTTC
Cuts at: 4748 4952 5700 6581 4748
Size: 204 748 881 6619
Fragments arranged by size:
6619 881 748 204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2689 6260 276
Size: 53 83 186 936 1155 3571 2468
Fragments arranged by size:
3571 2468 1155 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SexAI
SfoI SrfI SwaI