Restriction map for
pFN21AE2693
(Circular) (Six-base) MAPSORT of: pFN21AE2693.seq Check: 2775 from: 1 to: 15727
pFN21AE2693 15727 bp
With 182 enzymes: SgfI *
August 25, 2017 13:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3334 3577 5114 6718 7894 9960 10803 10962 12648
Size: 243 1537 1604 1176 2066 843 159 1686
Cuts at: 12648 3334
Size: 6413
Fragments arranged by size:
6413 2066 1686 1604 1537 1176 843 243
159
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 13537 278
Size: 53 83 186 936 12001 2468
Fragments arranged by size:
12001 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 13009 13070 1937
Size: 11072 61 4594
Fragments arranged by size:
11072 4594 61
Acc65I G'GTAC_C
Cuts at: 9069 12599 13048 9069
Size: 3530 449 11748
Fragments arranged by size:
11748 3530 449
AclI AA'CG_TT
Cuts at: 1563 9772 13854 14227 1563
Size: 8209 4082 373 3063
Fragments arranged by size:
8209 4082 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2885 3545 3989 4988 5276 5603 7964 8309
Size: 1253 660 444 999 288 327 2361 345
Cuts at: 8309 8360 8540 8579 8613 9616 9991 10604 10684
Size: 51 180 39 34 1003 375 613 80
Cuts at: 10684 11118 12356 12509 13790 15174 15442 1632
Size: 434 1238 153 1281 1384 268 1917
Fragments arranged by size:
2361 1917 1384 1281 1253 1238 1003 999
660 613 444 434 375 345 327 288
268 180 153 80 51 39 34
AfeI AGC'GCT
Cuts at: 2807 2807
Size: 15727
AflII C'TTAA_G
Cuts at: 820 1017 3329 12835 820
Size: 197 2312 9506 3712
Fragments arranged by size:
9506 3712 2312 197
AflIII A'CryG_T
Cuts at: 1203 6106 9103 9105 14626 15603 1203
Size: 4903 2997 2 5521 977 1327
Fragments arranged by size:
5521 4903 2997 1327 977 2
AgeI A'CCGG_T
Cuts at: 14558 14558
Size: 15727
AhdI GACnn_n'nnGTC
Cuts at: 14456 14456
Size: 15727
AleI CACnn'nnGTG
Cuts at: 1166 2591 3821 7856 9936 1166
Size: 1425 1230 4035 2080 6957
Fragments arranged by size:
6957 4035 2080 1425 1230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2733 5036 5862 7874 12267 1184
Size: 1549 2303 826 2012 4393 4644
Fragments arranged by size:
4644 4393 2303 2012 1549 826
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2070 2102 12450 12482 2070
Size: 32 10348 32 5315
Fragments arranged by size:
10348 5315 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2749 2767 3037 3227 3244 3409 3460 3697 3874
Size: 18 270 190 17 165 51 237 177
Cuts at: 3874 3919 4716 5221 5251 5344 5422 5485 5581
Size: 45 797 505 30 93 78 63 96
Cuts at: 5581 5614 5935 6355 6373 6814 6868 7447 7645
Size: 33 321 420 18 441 54 579 198
Cuts at: 7645 7687 7717 7900 8518 8692 8701 8755 8863
Size: 42 30 183 618 174 9 54 108
Cuts at: 8863 9172 9208 9469 9610 9655 9676 9730 10222
Size: 309 36 261 141 45 21 54 492
Cuts at: 10222 10243 10459 10777 10816 10933 11017 11413 11473
Size: 21 216 318 39 117 84 396 60
Cuts at: 11473 11669 11932 11980 12010 12121 12142 12196 12217
Size: 196 263 48 30 111 21 54 21
Cuts at: 12217 12289 12487 12640 12675 12934 13470 15042 2749
Size: 72 198 153 35 259 536 1572 3434
Fragments arranged by size:
3434 1572 797 618 579 536 505 492
441 420 396 321 318 309 270 263
261 259 237 216 198 198 196 190
183 177 174 165 153 141 117 111
108 96 93 84 78 72 63 60
54 54 54 54 51 48 45 45
42 39 36 35 33 30 30 30
21 21 21 21 18 18 17 9
ApaI G_GGCC'C
Cuts at: 3895 3904 4512 4654 4675 5290 6328 6862 7816
Size: 9 608 142 21 615 1038 534 954
Cuts at: 7816 8512 9382 12235 3895
Size: 696 870 2853 7387
Fragments arranged by size:
7387 2853 1038 954 870 696 615 608
534 142 21 9
ApaLI G'TGCA_C
Cuts at: 2339 9242 11529 12979 13784 14940 2339
Size: 6903 2287 1450 805 1156 3126
Fragments arranged by size:
6903 3126 2287 1450 1156 805
ApoI r'AATT_y
Cuts at: 1493 10177 13036 13262 13414 15523 1493
Size: 8684 2859 226 152 2109 1697
Fragments arranged by size:
8684 2859 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 14281 160
Size: 872 13249 1606
Fragments arranged by size:
13249 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15727
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2039 2317 2774 4151 4200 4443
Size: 73 85 11 278 457 1377 49 243
Cuts at: 4443 5790 7292 7488 7986 8358 8667 9114 9308
Size: 1347 1502 196 498 372 309 447 194
Cuts at: 9308 9947 10851 10920 11963 12494 13052 1870
Size: 639 904 69 1043 531 558 4545
Fragments arranged by size:
4545 1502 1377 1347 1043 904 639 558
531 498 457 447 372 309 278 243
196 194 85 73 69 49 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 9092 9125 9092
Size: 33 15694
BamHI G'GATC_C
Cuts at: 4147 8496 8814 11039 13057 13424 4147
Size: 4349 318 2225 2018 367 6450
Fragments arranged by size:
6450 4349 2225 2018 367 318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2052 2389 2562 3815 4031
Size: 325 217 627 265 337 173 1253 216
Cuts at: 4031 4359 5292 5539 6384 6665 6774 7664 8352
Size: 328 933 247 845 281 109 890 688
Cuts at: 8352 9069 9624 9942 9972 10791 11513 11769 12599
Size: 717 555 318 30 819 722 256 830
Cuts at: 12599 13048 14503 618
Size: 449 1455 1842
Fragments arranged by size:
1842 1455 1253 933 890 845 830 819
722 717 688 627 555 449 337 328
325 318 281 265 256 247 217 216
173 109 30
BanII G_rGCy'C
Cuts at: 1391 2037 2292 2368 3367 3766 3895 3904 4481
Size: 646 255 76 999 399 129 9 577
Cuts at: 4481 4512 4654 4675 4728 5096 5290 6027 6249
Size: 31 142 21 53 368 194 737 222
Cuts at: 6249 6328 6338 6603 6699 6862 7046 7816 8083
Size: 79 10 265 96 163 184 770 267
Cuts at: 8083 8243 8512 9382 9478 9494 9755 9843 10047
Size: 160 269 870 96 16 261 88 204
Cuts at: 10047 10419 10441 10479 10647 11028 11676 12235 12468
Size: 372 22 38 168 381 648 559 233
Cuts at: 12468 12787 12910 12941 13046 1391
Size: 319 123 31 105 4072
Fragments arranged by size:
4072 999 870 770 737 648 646 577
559 399 381 372 368 319 269 267
265 261 255 233 222 204 194 184
168 163 160 142 129 123 105 96
96 88 79 76 53 38 31 31
22 21 16 10 9
BbeI G_GCGC'C
Cuts at: 4035 4363 5296 8356 11773 4035
Size: 328 933 3060 3417 7989
Fragments arranged by size:
7989 3417 3060 933 328
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2183 2197 2579 5141 8275 8348 9798
Size: 971 284 14 382 2562 3134 73 1450
Cuts at: 9798 11702 928
Size: 1904 4953
Fragments arranged by size:
4953 3134 2562 1904 1450 971 382 284
73 14
BbvCI CC'TCA_GC
Cuts at: 4062 4118 4355 4857 5426 7221 8487 10097 10533
Size: 56 237 502 569 1795 1266 1610 436
Cuts at: 10533 12033 12300 4062
Size: 1500 267 7489
Fragments arranged by size:
7489 1795 1610 1500 1266 569 502 436
267 237 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8249 8283 11234 11268 12260 12294 13918
Size: 34 6312 34 2951 34 992 34 1624
Cuts at: 13918 13952 1903
Size: 34 3678
Fragments arranged by size:
6312 3678 2951 1624 992 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5888 13619 14829 5888
Size: 7731 1210 6786
Fragments arranged by size:
7731 6786 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 15727
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2223 3079 3104
Size: 107 122 71 631 605 551 856 25
Cuts at: 3104 3380 3899 4397 4536 5285 6428 6446 6470
Size: 276 519 498 139 749 1143 18 24
Cuts at: 6470 7877 8588 10271 10367 10529 10673 11108 11838
Size: 1407 711 1683 96 162 144 435 730
Cuts at: 11838 12311 12813 14338 136
Size: 473 502 1525 1525
Fragments arranged by size:
1683 1525 1525 1407 1143 856 749 730
711 631 605 551 519 502 498 473
435 276 162 144 139 122 107 96
71 25 24 18
BglII A'GATC_T
Cuts at: 2481 4093 7537 9187 15722 2481
Size: 1612 3444 1650 6535 2486
Fragments arranged by size:
6535 3444 2486 1650 1612
BlpI GC'TnA_GC
Cuts at: 2739 2760 3611 3783 4572 4875 5373 5415 9677
Size: 21 851 172 789 303 498 42 4262
Cuts at: 9677 11052 11670 12051 13144 2739
Size: 1375 618 381 1093 5322
Fragments arranged by size:
5322 4262 1375 1093 851 789 618 498
381 303 172 42 21
Bme1580I G_kGCm'C
Cuts at: 1790 2055 2343 3818 3895 3904 4512 4654 4675
Size: 265 288 1475 77 9 608 142 21
Cuts at: 4675 5290 5544 5812 6328 6670 6777 6862 7147
Size: 615 254 268 516 342 107 85 285
Cuts at: 7147 7410 7667 7816 7831 7957 8122 8512 9246
Size: 263 257 149 15 126 165 390 734
Cuts at: 9246 9382 9627 9945 9964 10487 10868 10896 11301
Size: 136 245 318 19 523 381 28 405
Cuts at: 11301 11533 11995 12235 12354 12983 13788 14944 1790
Size: 232 462 240 119 629 805 1156 2573
Fragments arranged by size:
2573 1475 1156 805 734 629 615 608
523 516 462 405 390 381 342 318
288 285 268 265 263 257 254 245
240 232 165 149 142 136 126 119
107 85 77 28 21 19 15 9
BmgBI CAC'GTC
Cuts at: 1318 2782 5059 5722 12040 1318
Size: 1464 2277 663 6318 5005
Fragments arranged by size:
6318 5005 2277 1464 663
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2088 2926 3190
Size: 416 53 83 399 24 671 838 264
Cuts at: 3190 6835 6856 8722 9609 10878 11846 13458 14416
Size: 3645 21 1866 887 1269 968 1612 958
Cuts at: 14416 442
Size: 1753
Fragments arranged by size:
3645 1866 1753 1612 1269 968 958 887
838 671 416 399 264 83 53 24
21
BmtI G_CTAG'C
Cuts at: 1056 11237 11987 1056
Size: 10181 750 4796
Fragments arranged by size:
10181 4796 750
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 3655 3820 5078 5235 8663 8682 9513 9580
Size: 1344 165 1258 157 3428 19 831 67
Cuts at: 9580 2311
Size: 8458
Fragments arranged by size:
8458 3428 1344 1258 831 165 157 67
19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2369 2641 2891 3011 3233 3440 3671
Size: 1067 63 272 250 120 222 207 231
Cuts at: 3671 3758 3929 4178 4328 4394 4436 4467 4534
Size: 87 171 249 150 66 42 31 67
Cuts at: 4534 4592 4712 4760 4811 4979 5051 5093 5168
Size: 58 120 48 51 168 72 42 75
Cuts at: 5168 5468 5648 5885 5930 5936 6233 6329 6346
Size: 300 180 237 45 6 297 96 17
Cuts at: 6346 6416 6530 6683 6857 6938 7060 7202 7637
Size: 70 114 153 174 81 122 142 435
Cuts at: 7637 7667 7712 7841 8000 8207 8777 8798 8885
Size: 30 45 129 159 207 570 21 87
Cuts at: 8885 9212 9251 9413 9752 9887 9899 9929 10058
Size: 327 39 162 339 135 12 30 129
Cuts at: 10058 10310 10337 10421 10433 10520 10691 10838 10955
Size: 252 27 84 12 87 171 147 117
Cuts at: 10955 11051 11147 11219 11354 11435 11540 11660 11795
Size: 96 96 72 135 81 105 120 135
Cuts at: 11795 12239 12311 12652 14387 1239
Size: 444 72 341 1735 2579
Fragments arranged by size:
2579 1735 1067 570 444 435 341 339
327 300 297 272 252 250 249 237
231 222 207 207 180 174 171 171
168 162 159 153 150 147 142 135
135 135 129 129 122 120 120 120
117 114 105 96 96 96 87 87
87 84 81 81 75 72 72 72
70 67 66 63 58 51 48 45
45 42 42 39 31 30 30 27
21 17 12 12 6
Bpu10I CC'TnA_GC
Cuts at: 4062 4118 4355 4475 4857 5426 5735 5774 6587
Size: 56 237 120 382 569 309 39 813
Cuts at: 6587 7098 7221 8487 10097 10533 10998 12033 12300
Size: 511 123 1266 1610 436 465 1035 267
Cuts at: 12300 4062
Size: 7489
Fragments arranged by size:
7489 1610 1266 1035 813 569 511 465
436 382 309 267 237 123 120 56
39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3729 4645 6044 8960 13852 14717 15015 3729
Size: 916 1399 2916 4892 865 298 4441
Fragments arranged by size:
4892 4441 2916 1399 916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2424 4340 6479 7508 8218 8686 11136
Size: 617 925 1916 2139 1029 710 468 2450
Cuts at: 11136 11336 14390 882
Size: 200 3054 2219
Fragments arranged by size:
3054 2450 2219 2139 1916 1029 925 710
617 468 200
BsaAI yAC'GTr
Cuts at: 493 1204 4366 6109 9106 13518 493
Size: 711 3162 1743 2997 4412 2702
Fragments arranged by size:
4412 3162 2997 2702 1743 711
BsaBI GATnn'nnATC
Cuts at: 15721 15721
Size: 15727
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4032 4360 4448 5293
Size: 53 83 186 936 2499 328 88 845
Cuts at: 5293 8353 10707 11358 11770 12014 13534 13916 14551
Size: 3060 2354 651 412 244 1520 382 635
Cuts at: 14551 275
Size: 1451
Fragments arranged by size:
3060 2499 2354 1520 1451 936 845 651
635 412 382 328 244 186 88 83
53
BsaWI w'CCGG_w
Cuts at: 2235 6029 6501 9066 9369 14160 14558 14832 14979
Size: 3794 472 2565 303 4791 398 274 147
Cuts at: 14979 15390 2235
Size: 411 2572
Fragments arranged by size:
4791 3794 2572 2565 472 411 398 303
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2669 2699 2831 2861 3753
Size: 30 165 30 1266 30 132 30 892
Cuts at: 3753 3783 4301 4331 4715 4745 6142 6172 6344
Size: 30 518 30 384 30 1397 30 172
Cuts at: 6344 6374 7028 7058 7856 7886 7982 8012 8877
Size: 30 654 30 798 30 96 30 865
Cuts at: 8877 8907 8941 8971 11999 12029 12450 12480 12815
Size: 30 34 30 3028 30 421 30 335
Cuts at: 12815 12845 1178
Size: 30 4060
Fragments arranged by size:
4060 3028 1397 1266 892 865 798 654
518 421 384 335 172 165 132 96
34 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2522 2893 3668 3796 3965 3968 4433
Size: 841 495 371 775 128 169 3 465
Cuts at: 4433 4697 5054 5167 5207 5465 5923 5935 5938
Size: 264 357 113 40 258 458 12 3
Cuts at: 5938 6947 7067 7925 8204 8624 9239 9489 10055
Size: 1009 120 858 279 420 615 250 566
Cuts at: 10055 10139 10307 10676 11342 11578 12038 12236 1186
Size: 84 168 369 666 236 460 198 4677
Fragments arranged by size:
4677 1009 858 841 775 666 615 566
495 465 460 458 420 371 369 357
279 264 258 250 236 198 169 168
128 120 113 84 40 12 3 3
BseYI C'CCAG_C
Cuts at: 2069 2632 2749 3201 3409 3504 3858 4567 5842
Size: 563 117 452 208 95 354 709 1275
Cuts at: 5842 6924 7815 8013 8017 8511 8685 10261 10297
Size: 1082 891 198 4 494 174 1576 36
Cuts at: 10297 10543 10861 10889 11007 11238 12079 12261 12318
Size: 246 318 28 118 231 841 182 57
Cuts at: 12318 14930 15426 2069
Size: 2612 496 2370
Fragments arranged by size:
2612 2370 1576 1275 1082 891 841 709
563 496 494 452 354 318 246 231
208 198 182 174 118 117 95 57
36 28 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2693 4790 5006 5032 5681 5832 6554
Size: 573 957 2097 216 26 649 151 722
Cuts at: 6554 7136 7289 7297 7730 7766 9221 9905 9914
Size: 582 153 8 433 36 1455 684 9
Cuts at: 9914 10751 10904 10946 11486 11900 11928 12365 1163
Size: 837 153 42 540 414 28 437 4525
Fragments arranged by size:
4525 2097 1455 957 837 722 684 649
582 573 540 437 433 414 216 153
153 151 42 36 28 26 9 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4341 4401 6944 8182 8919 13177
Size: 275 186 2340 60 2543 1238 737 4258
Cuts at: 13177 13938 14966 1540
Size: 761 1028 2301
Fragments arranged by size:
4258 2543 2340 2301 1238 1028 761 737
275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2037 2292 2343 2368 4728 5064 5096 5436 5780
Size: 255 51 25 2360 336 32 340 344
Cuts at: 5780 6249 6338 6603 6621 6699 7046 7299 8243
Size: 469 89 265 18 78 347 253 944
Cuts at: 8243 8871 9246 9755 9907 10047 10306 10479 11028
Size: 628 375 509 152 140 259 173 549
Cuts at: 11028 11533 11676 12468 12562 12983 13788 13873 14944
Size: 505 143 792 94 421 805 85 1071
Cuts at: 14944 2037
Size: 2820
Fragments arranged by size:
2820 2360 1071 944 805 792 628 549
509 505 469 421 375 347 344 340
336 265 259 255 253 173 152 143
140 94 89 85 78 51 32 25
18
BsmI GAATG_Cn'
Cuts at: 3044 4000 13238 13331 3044
Size: 956 9238 93 5440
Fragments arranged by size:
9238 5440 956 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2120 2830 5049 6966 12566 2120
Size: 710 2219 1917 5600 5281
Fragments arranged by size:
5600 5281 2219 1917 710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2055 2292 2343 2368 3367 3766
Size: 399 247 18 237 51 25 999 399
Cuts at: 3766 3818 3895 3904 4481 4512 4654 4675 4728
Size: 52 77 9 577 31 142 21 53
Cuts at: 4728 5064 5096 5290 5436 5544 5780 5812 6027
Size: 336 32 194 146 108 236 32 215
Cuts at: 6027 6249 6328 6338 6603 6621 6670 6699 6777
Size: 222 79 10 265 18 49 29 78
Cuts at: 6777 6862 7046 7147 7299 7410 7667 7816 7831
Size: 85 184 101 152 111 257 149 15
Cuts at: 7831 7957 8083 8122 8243 8512 8871 9246 9382
Size: 126 126 39 121 269 359 375 136
Cuts at: 9382 9478 9494 9627 9755 9843 9907 9945 9964
Size: 96 16 133 128 88 64 38 19
Cuts at: 9964 10047 10306 10419 10441 10479 10487 10647 10868
Size: 83 259 113 22 38 8 160 221
Cuts at: 10868 10896 11028 11301 11533 11676 11995 12235 12354
Size: 28 132 273 232 143 319 240 119
Cuts at: 12354 12468 12562 12787 12910 12941 12983 13046 13788
Size: 114 94 225 123 31 42 63 742
Cuts at: 13788 13873 14944 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 999 742 577 399 399 375
359 336 319 273 269 265 259 257
247 240 237 236 232 225 222 221
215 194 184 160 152 149 146 143
142 136 133 132 128 126 126 123
121 119 114 113 111 108 101 96
94 88 85 85 83 79 78 77
64 63 53 52 51 49 42 39
38 38 32 32 31 31 29 28
25 22 21 19 18 18 16 15
10 9 8
BspEI T'CCGG_A
Cuts at: 2235 6029 6501 9369 15390 2235
Size: 3794 472 2868 6021 2572
Fragments arranged by size:
6021 3794 2868 2572 472
BspHI T'CATG_A
Cuts at: 2814 3166 8616 13616 15346 2814
Size: 352 5450 5000 1730 3195
Fragments arranged by size:
5450 5000 3195 1730 352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3334 3577 3741 5114 5451 6115 6363 6718
Size: 2490 243 164 1373 337 664 248 355
Cuts at: 6718 7236 7894 7908 9960 10803 10962 12129 12648
Size: 518 658 14 2052 843 159 1167 519
Cuts at: 12648 13082 844
Size: 434 3489
Fragments arranged by size:
3489 2490 2052 1373 1167 843 664 658
519 518 434 355 337 248 243 164
159 14
BsrBI CCG'CTC
Cuts at: 1394 1823 4294 7672 7825 8335 9235 9497 11056
Size: 429 2471 3378 153 510 900 262 1559
Cuts at: 11056 11173 11524 13614 1394
Size: 117 351 2090 3507
Fragments arranged by size:
3507 3378 2471 2090 1559 900 510 429
351 262 153 117
BsrDI GCAATG_nn'
Cuts at: 65 1248 2754 8739 9441 14222 14396 65
Size: 1183 1506 5985 702 4781 174 1396
Fragments arranged by size:
5985 4781 1506 1396 1183 702 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3667 7374 8370 8527 14371 14558 1700
Size: 110 1857 3707 996 157 5844 187 2869
Fragments arranged by size:
5844 3707 2869 1857 996 187 157 110
BsrGI T'GTAC_A
Cuts at: 96 2244 96
Size: 2148 13579
BssHII G'CGCG_C
Cuts at: 1925 4387 6035 1925
Size: 2462 1648 11617
Fragments arranged by size:
11617 2462 1648
BssSI C'ACGA_G
Cuts at: 4097 7576 7658 8276 9221 9487 10838 10907 13787
Size: 3479 82 618 945 266 1351 69 2880
Cuts at: 13787 14799 4097
Size: 1012 5025
Fragments arranged by size:
5025 3479 2880 1351 1012 945 618 266
82 69
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3227 3607 3733 3919 4753 5251 5614 6355
Size: 2433 380 126 186 834 498 363 741
Cuts at: 6355 6739 7303 7447 7687 8036 8701 8875 9208
Size: 384 564 144 240 349 665 174 333
Cuts at: 9208 9448 9610 9862 10222 11305 12640 12643 794
Size: 240 162 252 360 1083 1335 3 3878
Fragments arranged by size:
3878 2433 1335 1083 834 741 665 564
498 384 380 363 360 349 333 252
240 240 186 174 162 144 126 3
BstBI TT'CG_AA
Cuts at: 14531 14531
Size: 15727
BstEII G'GTnAC_C
Cuts at: 1184 8846 1184
Size: 7662 8065
BstXI CCAn_nnnn'nTGG
Cuts at: 3853 4512 6257 7594 8017 8233 10766 3853
Size: 659 1745 1337 423 216 2533 8814
Fragments arranged by size:
8814 2533 1745 1337 659 423 216
BstYI r'GATC_y
Cuts at: 1972 2232 2481 2892 4012 4093 4147 5454 7444
Size: 260 249 411 1120 81 54 1307 1990
Cuts at: 7444 7537 8193 8496 8814 9187 11039 12858 13057
Size: 93 656 303 318 373 1852 1819 199
Cuts at: 13057 13424 13809 13826 15278 15364 15376 15722 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1990 1977 1852 1819 1452 1307 1120 656
411 385 373 367 346 318 303 260
249 199 93 86 81 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2378 4496 6278 6769 6989 7193 7211 7731 8723
Size: 2118 1782 491 220 204 18 520 992
Cuts at: 8723 8741 8991 9326 10637 11498 2378
Size: 18 250 335 1311 861 6607
Fragments arranged by size:
6607 2118 1782 1311 992 861 520 491
335 250 220 204 18 18
BtgI C'CryG_G
Cuts at: 513 1065 2004 2109 3757 4689 5505 6444 6451
Size: 552 939 105 1648 932 816 939 7
Cuts at: 6451 6628 7047 7422 9052 9319 9834 9915 9922
Size: 177 419 375 1630 267 515 81 7
Cuts at: 9922 10944 12609 12688 12811 513
Size: 1022 1665 79 123 3429
Fragments arranged by size:
3429 1665 1648 1630 1022 939 939 932
816 552 515 419 375 267 177 123
105 81 79 7 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2640 10016 487
Size: 683 56 1414 7376 6198
Fragments arranged by size:
7376 6198 1414 683 56
BtsI GCAGTG_nn'
Cuts at: 2705 4824 5240 5615 5664 5993 6809 7145 7788
Size: 2119 416 375 49 329 816 336 643
Cuts at: 7788 7939 8869 8966 10515 10538 10576 12918 13243
Size: 151 930 97 1549 23 38 2342 325
Cuts at: 13243 14037 14057 2705
Size: 794 20 4375
Fragments arranged by size:
4375 2342 2119 1549 930 816 794 643
416 375 336 329 325 151 97 49
38 23 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 15727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 15692
DraI TTT'AAA
Cuts at: 13031 13031
Size: 15727
DraIII CAC_nnn'GTG
Cuts at: 2190 5000 5002 6796 7342 10912 12358 2190
Size: 2810 2 1794 546 3570 1446 5559
Fragments arranged by size:
5559 3570 2810 1794 1446 546 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7459 14734 809
Size: 507 6143 7275 1802
Fragments arranged by size:
7275 6143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 3381 3669
Size: 54 1425 50 275 18 385 1166 288
Cuts at: 3669 4338 4398 5644 6160 6459 6471 6531 6941
Size: 669 60 1246 516 299 12 60 410
Cuts at: 6941 7161 7365 8179 8457 8916 10344 10749 10768
Size: 220 204 814 278 459 1428 405 19
Cuts at: 10768 10914 11379 11871 13174 14063 15692 8
Size: 146 465 492 1303 889 1629 43
Fragments arranged by size:
1629 1428 1425 1303 1246 1166 889 814
669 516 492 465 459 410 405 385
299 288 278 275 220 204 146 60
60 54 50 43 19 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 4338 4398 6941 8179 8916 13174 1537
Size: 275 2526 60 2543 1238 737 4258 4090
Fragments arranged by size:
4258 4090 2543 2526 1238 737 275 60
EarI CTCTTCn'nnn_
Cuts at: 1353 3119 4964 5991 6477 8064 8168 8244 9528
Size: 1766 1845 1027 486 1587 104 76 1284
Cuts at: 9528 10010 10593 11430 12069 12417 12876 13657 1353
Size: 482 583 837 639 348 459 781 3423
Fragments arranged by size:
3423 1845 1766 1587 1284 1027 837 781
639 583 486 482 459 348 104 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4283 6769 7400 7988 8432 8782
Size: 1064 138 2694 2486 631 588 444 350
Cuts at: 8782 10202 10399 11300 14314 14688 14834 387
Size: 1420 197 901 3014 374 146 1280
Fragments arranged by size:
3014 2694 2486 1420 1280 1064 901 631
588 444 374 350 197 146 138
EcoICRI GAG'CTC
Cuts at: 2035 2290 2366 4726 5094 6247 6336 6601 6697
Size: 255 76 2360 368 1153 89 265 96
Cuts at: 6697 7044 8241 9753 10045 10477 11026 11674 12466
Size: 347 1197 1512 292 432 549 648 792
Cuts at: 12466 2035
Size: 5296
Fragments arranged by size:
5296 2360 1512 1197 1153 792 648 549
432 368 347 292 265 255 96 89
76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2369 2641 2885 2891 3011 3233
Size: 393 674 63 272 244 6 120 222
Cuts at: 3233 3440 3545 3671 3758 3929 3989 4178 4328
Size: 207 105 126 87 171 60 189 150
Cuts at: 4328 4394 4436 4467 4534 4592 4712 4760 4811
Size: 66 42 31 67 58 120 48 51
Cuts at: 4811 4979 4988 5051 5093 5168 5276 5468 5603
Size: 168 9 63 42 75 108 192 135
Cuts at: 5603 5648 5885 5930 5936 6233 6329 6346 6416
Size: 45 237 45 6 297 96 17 70
Cuts at: 6416 6530 6683 6857 6938 7060 7202 7637 7667
Size: 114 153 174 81 122 142 435 30
Cuts at: 7667 7712 7841 7964 8000 8207 8309 8360 8540
Size: 45 129 123 36 207 102 51 180
Cuts at: 8540 8579 8613 8777 8798 8885 9212 9251 9413
Size: 39 34 164 21 87 327 39 162
Cuts at: 9413 9616 9752 9887 9899 9929 9991 10058 10310
Size: 203 136 135 12 30 62 67 252
Cuts at: 10310 10337 10421 10433 10520 10604 10684 10691 10838
Size: 27 84 12 87 84 80 7 147
Cuts at: 10838 10955 11051 11118 11147 11219 11354 11435 11540
Size: 117 96 67 29 72 135 81 105
Cuts at: 11540 11660 11795 12239 12311 12356 12509 12652 13790
Size: 120 135 444 72 45 153 143 1138
Cuts at: 13790 14387 15174 15442 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1138 787 674 597 444 435 393
327 297 272 268 252 244 237 222
207 207 203 192 189 180 174 171
168 164 162 153 153 150 147 143
142 136 135 135 135 135 129 126
123 122 120 120 120 117 114 108
105 105 102 96 96 87 87 87
84 84 81 81 80 75 72 72
70 67 67 67 66 63 63 62
60 58 51 51 48 45 45 45
45 42 42 39 39 36 34 31
30 30 29 27 21 17 12 12
9 7 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2377 2858 3437 3485 3620 3737 5223 5447 5465
Size: 481 579 48 135 117 1486 224 18
Cuts at: 5465 5714 6122 7214 7283 7556 8726 8804 8855
Size: 249 408 1092 69 273 1170 78 51
Cuts at: 8855 8944 9164 9932 10564 11477 11708 11873 12125
Size: 89 220 768 632 913 231 165 252
Cuts at: 12125 2377
Size: 5979
Fragments arranged by size:
5979 1486 1170 1092 913 768 632 579
481 408 273 252 249 231 224 220
165 135 117 89 78 69 51 48
18
EcoO109I rG'GnC_Cy
Cuts at: 2086 2305 3106 3153 3172 3241 3457 3891 3901
Size: 219 801 47 19 69 216 434 10
Cuts at: 3901 4051 4131 4508 4509 4651 4671 4846 4963
Size: 150 80 377 1 142 20 175 117
Cuts at: 4963 5077 5557 5731 5786 6115 6153 6324 6325
Size: 114 480 174 55 329 38 171 1
Cuts at: 6325 6352 6541 7188 7225 7288 7603 7727 7812
Size: 27 189 647 37 63 315 124 85
Cuts at: 7812 7897 8542 8698 8719 8860 8986 9090 9169
Size: 85 645 156 21 141 126 104 79
Cuts at: 9169 9378 9693 9727 9820 9874 10020 10369 10456
Size: 209 315 34 93 54 146 349 87
Cuts at: 10456 11110 11410 11866 12118 12232 12265 12534 12580
Size: 654 300 456 252 114 33 269 46
Cuts at: 12580 12693 2086
Size: 113 5120
Fragments arranged by size:
5120 801 654 647 645 480 456 434
377 349 329 315 315 300 269 252
219 216 209 189 175 174 171 156
150 146 142 141 126 124 117 114
114 113 104 93 87 85 85 80
79 69 63 55 54 47 46 38
37 34 33 27 21 20 19 10
1 1
EcoRI G'AATT_C
Cuts at: 13036 13414 13036
Size: 378 15349
EcoRV GAT'ATC
Cuts at: 4895 4895
Size: 15727
FspI TGC'GCA
Cuts at: 3941 5381 6018 14233 3941
Size: 1440 637 8215 5435
Fragments arranged by size:
8215 5435 1440 637
HaeII r_GCGC'y
Cuts at: 1678 2809 3295 3451 4035 4125 4363 5296 8356
Size: 1131 486 156 584 90 238 933 3060
Cuts at: 8356 8395 9401 9462 10004 11542 11773 11971 14874
Size: 39 1006 61 542 1538 231 198 2903
Cuts at: 14874 1678
Size: 2531
Fragments arranged by size:
3060 2903 2531 1538 1131 1006 933 584
542 486 238 231 198 156 90 61
39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 2792 2824 4101
Size: 32 135 32 1097 32 91 32 1277
Cuts at: 4101 4133 4283 4301 4315 4333 5393 5425 6344
Size: 32 150 18 14 18 1060 32 919
Cuts at: 6344 6376 7856 7888 8877 8909 9889 9921 11024
Size: 32 1480 32 989 32 980 32 1103
Cuts at: 11024 11056 11999 12031 12996 13028 14368 14400 14442
Size: 32 943 32 965 32 1340 32 42
Cuts at: 14442 14474 1373
Size: 32 2626
Fragments arranged by size:
2626 1480 1340 1277 1103 1097 1060 989
980 965 943 919 150 135 91 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 18
18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 13071 13317 669
Size: 988 281 11133 246 3079
Fragments arranged by size:
11133 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6210 12275 748
Size: 5462 6065 4200
Fragments arranged by size:
6065 5462 4200
HpaI GTT'AAC
Cuts at: 13317 13317
Size: 15727
KasI G'GCGC_C
Cuts at: 4031 4359 5292 8352 11769 4031
Size: 328 933 3060 3417 7989
Fragments arranged by size:
7989 3417 3060 933 328
KpnI G_GTAC'C
Cuts at: 9073 12603 13052 9073
Size: 3530 449 11748
Fragments arranged by size:
11748 3530 449
MfeI C'AATT_G
Cuts at: 13326 13326
Size: 15727
MluI A'CGCG_T
Cuts at: 15603 15603
Size: 15727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 3889 4659 4942 7443 8908 9663 11574
Size: 1350 1234 770 283 2501 1465 755 1911
Cuts at: 11574 14841 15025 1305
Size: 3267 184 2007
Fragments arranged by size:
3267 2501 2007 1911 1465 1350 1234 770
755 283 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3383 6162 6533 8459 10346
Size: 54 1425 343 1551 2779 371 1926 1887
Cuts at: 10346 10770 11873 10
Size: 424 1103 3864
Fragments arranged by size:
3864 2779 1926 1887 1551 1425 1103 424
371 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2591 3821 7856 9936 10145 13685
Size: 648 999 426 1230 4035 2080 209 3540
Cuts at: 13685 14044 14203 518
Size: 359 159 2042
Fragments arranged by size:
4035 3540 2080 2042 1230 999 648 426
359 209 159
MspA1I CmG'CkG
Cuts at: 1606 2569 2764 2944 2989 3265 3271 3504 3649
Size: 963 195 180 45 276 6 233 145
Cuts at: 3649 3697 3925 3985 4189 4571 4957 4990 5050
Size: 48 228 60 204 382 386 33 60
Cuts at: 5050 5071 5122 5377 5419 5485 5846 5869 6077
Size: 21 51 255 42 66 361 23 208
Cuts at: 6077 6286 6307 6394 6550 6946 7138 7360 7627
Size: 209 21 87 156 396 192 222 267
Cuts at: 7627 7678 7819 7942 8017 8095 8518 8689 9016
Size: 51 141 123 75 78 423 171 327
Cuts at: 9016 9620 9718 9742 9924 10054 10228 10754 10933
Size: 604 98 24 182 130 174 526 179
Cuts at: 10933 11242 11317 11332 11669 12142 12565 12684 12690
Size: 309 75 15 337 473 423 119 6
Cuts at: 12690 12743 13143 13820 14968 15213 1606
Size: 53 400 677 1148 245 2120
Fragments arranged by size:
2120 1148 963 677 604 526 473 423
423 400 396 386 382 361 337 327
309 276 267 255 245 233 228 222
209 208 204 195 192 182 180 179
174 171 156 145 141 130 123 119
98 87 78 75 75 66 60 60
53 51 51 48 45 42 33 24
23 21 21 15 6 6
NaeI GCC'GGC
Cuts at: 3669 7376 3669
Size: 3707 12020
NarI GG'CG_CC
Cuts at: 4032 4360 5293 8353 11770 4032
Size: 328 933 3060 3417 7989
Fragments arranged by size:
7989 3417 3060 933 328
NcoI C'CATG_G
Cuts at: 513 1065 2004 2109 4689 5505 6444 6451 6628
Size: 552 939 105 2580 816 939 7 177
Cuts at: 6628 7047 7422 9052 9834 10944 12609 513
Size: 419 375 1630 782 1110 1665 3631
Fragments arranged by size:
3631 2580 1665 1630 1110 939 939 816
782 552 419 375 177 105 7
NdeI CA'TA_TG
Cuts at: 387 387
Size: 15727
NgoMIV G'CCGG_C
Cuts at: 3667 7374 3667
Size: 3707 12020
NheI G'CTAG_C
Cuts at: 1052 11233 11983 1052
Size: 10181 750 4796
Fragments arranged by size:
10181 4796 750
NotI GC'GGCC_GC
Cuts at: 4338 6941 8179 13174 4338
Size: 2603 1238 4995 6891
Fragments arranged by size:
6891 4995 2603 1238
NspI r_CATG'y
Cuts at: 1132 1229 2164 3418 3601 6625 6849 8444 10207
Size: 97 935 1254 183 3024 224 1595 1763
Cuts at: 10207 13085 14630 1132
Size: 2878 1545 2229
Fragments arranged by size:
3024 2878 2229 1763 1595 1545 1254 935
224 183 97
PasI CC'CwG_GG
Cuts at: 1348 3811 4003 4505 4541 5782 9964 12910 1348
Size: 2463 192 502 36 1241 4182 2946 4165
Fragments arranged by size:
4182 4165 2946 2463 1241 502 192 36
PciI A'CATG_T
Cuts at: 14626 14626
Size: 15727
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3409 3460 3649 5176 5506 6394 6457 6814
Size: 2301 51 189 1527 330 888 63 357
Cuts at: 6814 7038 7363 7645 8755 8863 9172 9655 10036
Size: 224 325 282 1110 108 309 483 381
Cuts at: 10036 10084 10594 10816 10912 11068 11932 12675 1108
Size: 48 510 222 96 156 864 743 4160
Fragments arranged by size:
4160 2301 1527 1110 888 864 743 510
483 381 357 330 325 309 282 224
222 189 156 108 96 63 51 48
PfoI T'CCnGG_A
Cuts at: 2346 2907 3315 4483 5952 6477 7128 7563 8064
Size: 561 408 1168 1469 525 651 435 501
Cuts at: 8064 8775 8817 9566 10278 11028 11067 11196 12723
Size: 711 42 749 712 750 39 129 1527
Cuts at: 12723 2346
Size: 5350
Fragments arranged by size:
5350 1527 1469 1168 750 749 712 711
651 561 525 501 435 408 129 42
39
PmeI GTTT'AAAC
Cuts at: 13031 13031
Size: 15727
PmlI CAC'GTG
Cuts at: 4366 6109 9106 4366
Size: 1743 2997 10987
Fragments arranged by size:
10987 2997 1743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12450 12482 13796 13828 15309 15341 12450
Size: 32 1314 32 1481 32 12836
Fragments arranged by size:
12836 1481 1314 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 5557 6352 7188 7225 7288 7603 7897 8542
Size: 3471 795 836 37 63 315 294 645
Cuts at: 8542 8719 8860 8986 9820 9874 10020 11410 12118
Size: 177 141 126 834 54 146 1390 708
Cuts at: 12118 12580 12693 2086
Size: 462 113 5120
Fragments arranged by size:
5120 3471 1390 836 834 795 708 645
462 315 294 177 146 141 126 113
63 54 37
PshAI GACnn'nnGTC
Cuts at: 1613 2822 1613
Size: 1209 14518
PsiI TTA'TAA
Cuts at: 13297 13297
Size: 15727
PspOMI G'GGCC_C
Cuts at: 3891 3900 4508 4650 4671 5286 6324 6858 7812
Size: 9 608 142 21 615 1038 534 954
Cuts at: 7812 8508 9378 12231 3891
Size: 696 870 2853 7387
Fragments arranged by size:
7387 2853 1038 954 870 696 615 608
534 142 21 9
PspXI vC'TCGA_Gb
Cuts at: 8667 8667
Size: 15727
PstI C_TGCA'G
Cuts at: 830 2050 2928 3249 3264 3270 3624 3663 3723
Size: 1220 878 321 15 6 354 39 60
Cuts at: 3723 3837 3924 4041 4230 4251 4712 5025 5085
Size: 114 87 117 189 21 461 313 60
Cuts at: 5085 5121 5247 5481 5637 5748 5874 6054 6123
Size: 36 126 234 156 111 126 180 69
Cuts at: 6123 6291 6378 6522 6549 6555 6873 7152 7233
Size: 168 87 144 27 6 318 279 81
Cuts at: 7233 7452 7683 7702 7791 7905 8044 8088 8142
Size: 219 231 19 89 114 139 44 54
Cuts at: 8142 8391 8697 8790 8880 8973 9444 9723 10059
Size: 249 306 93 90 93 471 279 336
Cuts at: 10059 10071 10227 10251 10653 10800 10992 11181 11247
Size: 12 156 24 402 147 192 189 66
Cuts at: 11247 11310 11322 11635 11757 11857 11904 12192 12213
Size: 63 12 313 122 100 47 288 21
Cuts at: 12213 12273 12477 12925 13079 830
Size: 60 204 448 154 3478
Fragments arranged by size:
3478 1220 878 471 461 448 402 354
336 321 318 313 313 306 288 279
279 249 234 231 219 204 192 189
189 180 168 156 156 154 147 144
139 126 126 122 117 114 114 111
100 93 93 90 89 87 87 81
69 66 63 60 60 60 54 47
44 39 36 27 24 21 21 19
15 12 12 6 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 15727
PvuII CAG'CTG
Cuts at: 2764 2944 2989 3265 3504 3649 3697 4189 4571
Size: 180 45 276 239 145 48 492 382
Cuts at: 4571 4957 4990 5050 5071 5122 5377 5485 5846
Size: 386 33 60 21 51 255 108 361
Cuts at: 5846 5869 6077 6286 6307 6394 6550 7138 7627
Size: 23 208 209 21 87 156 588 489
Cuts at: 7627 7678 7819 8017 8518 9718 9742 10054 10933
Size: 51 141 198 501 1200 24 312 879
Cuts at: 10933 11242 11317 11332 11669 12142 12565 12684 2764
Size: 309 75 15 337 473 423 119 5807
Fragments arranged by size:
5807 1200 879 588 501 492 489 473
423 386 382 361 337 312 309 276
255 239 209 208 198 180 156 145
141 119 108 87 75 60 51 51
48 45 33 24 23 21 21 15
SacI G_AGCT'C
Cuts at: 2037 2292 2368 4728 5096 6249 6338 6603 6699
Size: 255 76 2360 368 1153 89 265 96
Cuts at: 6699 7046 8243 9755 10047 10479 11028 11676 12468
Size: 347 1197 1512 292 432 549 648 792
Cuts at: 12468 2037
Size: 5296
Fragments arranged by size:
5296 2360 1512 1197 1153 792 648 549
432 368 347 292 265 255 96 89
76
SacII CC_GC'GG
Cuts at: 9925 12691 9925
Size: 2766 12961
SalI G'TCGA_C
Cuts at: 1936 13069 1936
Size: 11133 4594
SanDI GG'GwC_CC
Cuts at: 2086 7188 8986 9820 10020 12580 2086
Size: 5102 1798 834 200 2560 5233
Fragments arranged by size:
5233 5102 2560 1798 834 200
SapI GCTCTTCn'nnn_
Cuts at: 3119 8064 8168 9528 10010 10593 12069 12876 3119
Size: 4945 104 1360 482 583 1476 807 5970
Fragments arranged by size:
5970 4945 1476 1360 807 583 482 104
SbfI CC_TGCA'GG
Cuts at: 7233 7905 8088 10071 10653 10800 11310 11904 12192
Size: 672 183 1983 582 147 510 594 288
Cuts at: 12192 13079 7233
Size: 887 9881
Fragments arranged by size:
9881 1983 887 672 594 582 510 288
183 147
ScaI AGT'ACT
Cuts at: 4253 6896 13975 15687 4253
Size: 2643 7079 1712 4293
Fragments arranged by size:
7079 4293 2643 1712
SexAI A'CCwGG_T
Cuts at: 8100 10110 8100
Size: 2010 13717
SfcI C'TryA_G
Cuts at: 826 1045 2046 2147 2924 3083 3122 3245 3260
Size: 219 1001 101 777 159 39 123 15
Cuts at: 3260 3266 3569 3620 3659 3719 3833 3920 4037
Size: 6 303 51 39 60 114 87 117
Cuts at: 4037 4226 4247 4708 5021 5081 5117 5243 5477
Size: 189 21 461 313 60 36 126 234
Cuts at: 5477 5633 5744 5870 6050 6119 6287 6329 6374
Size: 156 111 126 180 69 168 42 45
Cuts at: 6374 6518 6545 6551 6869 7016 7148 7229 7448
Size: 144 27 6 318 147 132 81 219
Cuts at: 7448 7679 7698 7787 7901 8040 8084 8138 8387
Size: 231 19 89 114 139 44 54 249
Cuts at: 8387 8693 8786 8876 8969 9440 9713 9719 9737
Size: 306 93 90 93 471 273 6 18
Cuts at: 9737 10055 10067 10223 10247 10649 10796 10988 11177
Size: 318 12 156 24 402 147 192 189
Cuts at: 11177 11243 11306 11318 11631 11753 11816 11853 11900
Size: 66 63 12 313 122 63 37 47
Cuts at: 11900 12188 12209 12269 12473 12921 13075 14210 14891
Size: 288 21 60 204 448 154 1135 681
Cuts at: 14891 15082 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1001 777 681 471 461 448
402 318 318 313 313 306 303 288
273 249 234 231 219 219 204 192
191 189 189 180 168 159 156 156
154 147 147 144 139 132 126 126
123 122 117 114 114 111 101 93
93 90 89 87 81 69 66 63
63 60 60 60 54 51 47 45
44 42 39 39 37 36 27 24
21 21 19 18 15 12 12 6
6 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 3104 3899 5285 10367 10673 12311 3104
Size: 795 1386 5082 306 1638 6520
Fragments arranged by size:
6520 5082 1638 1386 795 306
SfoI GGC'GCC
Cuts at: 4033 4361 5294 8354 11771 4033
Size: 328 933 3060 3417 7989
Fragments arranged by size:
7989 3417 3060 933 328
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15727
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 15727
SmaI CCC'GGG
Cuts at: 1872 2030 4153 4202 4445 7988 8360 10853 10922
Size: 158 2123 49 243 3543 372 2493 69
Cuts at: 10922 12496 13054 1872
Size: 1574 558 4545
Fragments arranged by size:
4545 3543 2493 2123 1574 558 372 243
158 69 49
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3329 3744 4624 6059 8667 8975
Size: 197 926 1386 415 880 1435 2608 308
Cuts at: 8975 12835 13831 14732 14994 820
Size: 3860 996 901 262 1553
Fragments arranged by size:
3860 2608 1553 1435 1386 996 926 901
880 415 308 262 197
SnaBI TAC'GTA
Cuts at: 493 13518 493
Size: 13025 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 15727
SphI G_CATG'C
Cuts at: 1132 3418 8444 10207 13085 1132
Size: 2286 5026 1763 2878 3774
Fragments arranged by size:
5026 3774 2878 2286 1763
SrfI GCCC'GGGC
Cuts at: 4445 4445
Size: 15727
SspI AAT'ATT
Cuts at: 5 52 13651 5
Size: 47 13599 2081
Fragments arranged by size:
13599 2081 47
StuI AGG'CCT
Cuts at: 1517 5690 6192 6557 8582 8810 10061 10361 10559
Size: 4173 502 365 2025 228 1251 300 198
Cuts at: 10559 11888 12305 1517
Size: 1329 417 4939
Fragments arranged by size:
4939 4173 2025 1329 1251 502 417 365
300 228 198
StyI C'CwwG_G
Cuts at: 513 1065 2004 2109 2385 2496 2541 3840 4689
Size: 552 939 105 276 111 45 1299 849
Cuts at: 4689 5505 5514 5710 6444 6451 6628 7047 7422
Size: 816 9 196 734 7 177 419 375
Cuts at: 7422 8055 8232 9052 9093 9825 9834 10119 10944
Size: 633 177 820 41 732 9 285 825
Cuts at: 10944 11226 11373 11484 11874 12061 12609 12987 13166
Size: 282 147 111 390 187 548 378 179
Cuts at: 13166 513
Size: 3074
Fragments arranged by size:
3074 1299 939 849 825 820 816 734
732 633 552 548 419 390 378 375
285 282 276 196 187 179 177 177
147 111 111 105 45 41 9 9
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3077 5856 6916 7571 8027 8065 8483
Size: 1255 1497 2779 1060 655 456 38 418
Cuts at: 8483 8921 10275 10717 11548 13754 13771 13924 14109
Size: 438 1354 442 831 2206 17 153 185
Cuts at: 14109 14554 325
Size: 445 1498
Fragments arranged by size:
2779 2206 1498 1497 1354 1255 1060 831
655 456 445 442 438 418 185 153
38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 4251 5263
Size: 274 80 33 51 1443 267 2007 1012
Cuts at: 5263 6894 13973 15685 96
Size: 1631 7079 1712 138
Fragments arranged by size:
7079 2007 1712 1631 1443 1012 274 267
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2831 2863 3753 3785 3972 4004 6142 6174 7982
Size: 32 890 32 187 32 2138 32 1808
Cuts at: 7982 8014 8941 8973 9095 9127 12815 12847 15687
Size: 32 927 32 122 32 3688 32 2840
Cuts at: 15687 15719 2831
Size: 32 2839
Fragments arranged by size:
3688 2840 2839 2138 1808 927 890 187
122 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 9128 1316
Size: 7812 7915
XbaI T'CTAG_A
Cuts at: 13063 13063
Size: 15727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4538 5512 7079 9656 10460 11143 4538
Size: 974 1567 2577 804 683 9122
Fragments arranged by size:
9122 2577 1567 974 804 683
XhoI C'TCGA_G
Cuts at: 1943 8667 1943
Size: 6724 9003
XmaI C'CCGG_G
Cuts at: 1870 2028 4151 4200 4443 7986 8358 10851 10920
Size: 158 2123 49 243 3543 372 2493 69
Cuts at: 10920 12494 13052 1870
Size: 1574 558 4545
Fragments arranged by size:
4545 3543 2493 2123 1574 558 372 243
158 69 49
XmnI GAAnn'nnTTC
Cuts at: 2180 8073 10697 13856 2180
Size: 5893 2624 3159 4051
Fragments arranged by size:
5893 4051 3159 2624
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 13535 276
Size: 53 83 186 936 12001 2468
Fragments arranged by size:
12001 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BstZ17I FalI FseI FspAI
NruI NsiI PacI PsrI RsrII SwaI