Restriction map for
pFN21AE2688
(Circular) (Six-base) MAPSORT of: pFN21AE2688.seq Check: 6097 from: 1 to: 8887
pFN21AE2688 8887 bp
With 182 enzymes: SgfI *
May 11, 2017 14:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3352 6697 278
Size: 53 83 186 936 1816 3345 2468
Fragments arranged by size:
3345 2468 1816 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4171 6230 1937
Size: 2234 2059 4594
Fragments arranged by size:
4594 2234 2059
Acc65I G'GTAC_C
Cuts at: 6208 6208
Size: 8887
AclI AA'CG_TT
Cuts at: 1563 7014 7387 1563
Size: 5451 373 3063
Fragments arranged by size:
5451 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 2923 3631 4337 4757 5604 6113 6950
Size: 815 476 708 706 420 847 509 837
Cuts at: 6950 8334 8602 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 847 837 815 708 706 509
476 420 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8690
AflIII A'CryG_T
Cuts at: 1203 2990 7786 8763 1203
Size: 1787 4796 977 1327
Fragments arranged by size:
4796 1787 1327 977
AgeI A'CCGG_T
Cuts at: 7718 7718
Size: 8887
AhdI GACnn_n'nnGTC
Cuts at: 7616 7616
Size: 8887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5091 5123 5091
Size: 32 8855
AlwNI CAG_nnn'CTG
Cuts at: 2872 5102 6630 8202 2872
Size: 2230 1528 1572 3557
Fragments arranged by size:
3557 2230 1572 1528
ApaI G_GGCC'C
Cuts at: 2145 2145
Size: 8887
ApaLI G'TGCA_C
Cuts at: 6944 8100 6944
Size: 1156 7731
ApoI r'AATT_y
Cuts at: 1493 2435 2537 2973 3072 3855 3952 4159 4211
Size: 942 102 436 99 783 97 207 52
Cuts at: 4211 5074 5884 6143 6196 6422 6574 8683 1493
Size: 863 810 259 53 226 152 2109 1697
Fragments arranged by size:
2109 1697 942 863 810 783 436 259
226 207 152 102 99 97 53 52
AseI AT'TA_AT
Cuts at: 160 1032 7441 160
Size: 872 6409 1606
Fragments arranged by size:
6409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8887
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 3726 6212 1870
Size: 73 152 1631 2486 4545
Fragments arranged by size:
4545 2486 1631 152 73
AvrII C'CTAG_G
Cuts at: 2356 2356
Size: 8887
BamHI G'GATC_C
Cuts at: 6217 6584 6217
Size: 367 8520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2648 6208 7663 618
Size: 325 217 627 861 3560 1455 1842
Fragments arranged by size:
3560 1842 1455 861 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 2168 2263 4759 5821 6206 1391
Size: 754 23 95 2496 1062 385 4072
Fragments arranged by size:
4072 2496 1062 754 385 95 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5517 928
Size: 971 3618 4298
Fragments arranged by size:
4298 3618 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7078 7112 1903
Size: 34 5141 34 3678
Fragments arranged by size:
5141 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2964 6779 7989 2964
Size: 3815 1210 3862
Fragments arranged by size:
3862 3815 1210
BclI T'GATC_A
Cuts at: 1548 3421 1548
Size: 1873 7014
BfrBI ATG'CAT
Cuts at: 2831 2831
Size: 8887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 7498 136
Size: 107 122 71 631 605 379 5447 1525
Fragments arranged by size:
5447 1525 631 605 379 122 107 71
BglII A'GATC_T
Cuts at: 2275 8882 2275
Size: 6607 2280
BlpI GC'TnA_GC
Cuts at: 6304 6304
Size: 8887
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2716 6948 8104 1790
Size: 355 571 4232 1156 2573
Fragments arranged by size:
4232 2573 1156 571 355
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2684 6618 7576
Size: 416 53 83 399 24 1267 3934 958
Cuts at: 7576 442
Size: 1753
Fragments arranged by size:
3934 1753 1267 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2894 3857 4728 6123 7547 1239
Size: 1655 963 871 1395 1424 2579
Fragments arranged by size:
2579 1655 1424 1395 963 871
Bpu10I CC'TnA_GC
Cuts at: 2347 2347
Size: 8887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3513 4550 7012 7877 8175 3513
Size: 1037 2462 865 298 4225
Fragments arranged by size:
4225 2462 1037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4860 5279 7550 882
Size: 617 3361 419 2271 2219
Fragments arranged by size:
3361 2271 2219 617 419
BsaAI yAC'GTr
Cuts at: 493 1204 2387 6678 493
Size: 711 1183 4291 2702
Fragments arranged by size:
4291 2702 1183 711
BsaBI GATnn'nnATC
Cuts at: 5717 8881 5717
Size: 3164 5723
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3349 6694 7076 7711
Size: 53 83 186 936 1816 3345 382 635
Cuts at: 7711 275
Size: 1451
Fragments arranged by size:
3345 1816 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7320 7718 7992 8139 8550 7320
Size: 398 274 147 411 7657
Fragments arranged by size:
7657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6096 6126 1178
Size: 30 165 30 4693 30 3939
Fragments arranged by size:
4693 3939 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2228 3821 5699 1186
Size: 886 156 1593 1878 4374
Fragments arranged by size:
4374 1878 1593 886 156
BseYI C'CCAG_C
Cuts at: 2167 2948 8090 8586 2167
Size: 781 5142 496 2468
Fragments arranged by size:
5142 2468 781 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8314
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6337 7098 8126 1540
Size: 275 186 4336 761 1028 2301
Fragments arranged by size:
4336 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4759 5821 6948 7033 8104 4759
Size: 1062 1127 85 1071 5542
Fragments arranged by size:
5542 1127 1071 1062 85
BsmI GAATG_Cn'
Cuts at: 2301 2758 2833 3402 3879 5179 5276 6398 6491
Size: 457 75 569 477 1300 97 1122 93
Cuts at: 6491 2301
Size: 4697
Fragments arranged by size:
4697 1300 1122 569 477 457 97 93
75
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2168 2263 2716 4759 5821 6206
Size: 399 355 23 95 453 2043 1062 385
Cuts at: 6206 6948 7033 8104 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 2043 1071 1062 742 453 399 385
355 95 85 23
BspEI T'CCGG_A
Cuts at: 8550 8550
Size: 8887
BspHI T'CATG_A
Cuts at: 4579 5976 6776 8506 4579
Size: 1397 800 1730 4960
Fragments arranged by size:
4960 1730 1397 800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2348 6242 844
Size: 1504 3894 3489
Fragments arranged by size:
3894 3489 1504
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 2132 2346 6774 1394
Size: 429 245 64 214 4428 3507
Fragments arranged by size:
4428 3507 429 245 214 64
BsrDI GCAATG_nn'
Cuts at: 65 1248 2184 3024 3335 3599 4641 5268 5550
Size: 1183 936 840 311 264 1042 627 282
Cuts at: 5550 7382 7556 65
Size: 1832 174 1396
Fragments arranged by size:
1832 1396 1183 1042 936 840 627 311
282 264 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 4247 7531 7718 1700
Size: 110 290 2147 3284 187 2869
Fragments arranged by size:
3284 2869 2147 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 3696 4495 96
Size: 3600 799 4488
Fragments arranged by size:
4488 3600 799
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8887
BssSI C'ACGA_G
Cuts at: 3477 4968 6947 7959 3477
Size: 1491 1979 1012 4405
Fragments arranged by size:
4405 1979 1491 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8887
BstBI TT'CG_AA
Cuts at: 2287 7691 2287
Size: 5404 3483
BstEII G'GTnAC_C
Cuts at: 1184 5012 1184
Size: 3828 5059
BstYI r'GATC_y
Cuts at: 1972 2021 2275 2683 6217 6584 6969 6986 8438
Size: 49 254 408 3534 367 385 17 1452
Cuts at: 8438 8524 8536 8882 1972
Size: 86 12 346 1977
Fragments arranged by size:
3534 1977 1452 408 385 367 346 254
86 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 5879 5879
Size: 8887
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 7255
Fragments arranged by size:
7255 939 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8148
Fragments arranged by size:
8148 683 56
BtsI GCAGTG_nn'
Cuts at: 5433 6403 7197 7217 5433
Size: 970 794 20 7103
Fragments arranged by size:
7103 970 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8852
DraI TTT'AAA
Cuts at: 6191 6191
Size: 8887
DraIII CAC_nnn'GTG
Cuts at: 3145 3145
Size: 8887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7894 809
Size: 507 6578 1802
Fragments arranged by size:
6578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2296 3332
Size: 54 1425 50 275 18 318 148 1036
Cuts at: 3332 5451 5736 6334 7223 8852 8
Size: 2119 285 598 889 1629 43
Fragments arranged by size:
2119 1629 1425 1036 889 598 318 285
275 148 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6334 1537
Size: 275 4522 4090
Fragments arranged by size:
4522 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2418 4074 4691 6187 6817 1353
Size: 1065 1656 617 1496 630 3423
Fragments arranged by size:
3423 1656 1496 1065 630 617
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5741 7474 7848 7994 387
Size: 1064 138 4152 1733 374 146 1280
Fragments arranged by size:
4152 1733 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4757 5819 4757
Size: 1062 7825
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 2894 2923 3631 3857 4337 4728
Size: 393 815 447 29 708 226 480 391
Cuts at: 4728 4757 5604 6113 6123 6950 7547 8334 8602
Size: 29 847 509 10 827 597 787 268
Cuts at: 8602 1239
Size: 1524
Fragments arranged by size:
1524 847 827 815 787 708 597 509
480 447 393 391 268 226 29 29
10
EcoNI CCTnn'n_nnAGG
Cuts at: 5006 5006
Size: 8887
EcoRI G'AATT_C
Cuts at: 5074 5884 6196 6574 5074
Size: 810 312 378 7387
Fragments arranged by size:
7387 810 378 312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2537 2537
Size: 8887
FspI TGC'GCA
Cuts at: 7393 7393
Size: 8887
HaeII r_GCGC'y
Cuts at: 1678 8034 1678
Size: 6356 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2230 2260 2262 2292 4741
Size: 32 135 32 658 30 2 30 2449
Cuts at: 4741 4773 5993 6025 6096 6128 7528 7560 7602
Size: 32 1220 32 71 32 1400 32 42
Cuts at: 7602 7634 1373
Size: 32 2626
Fragments arranged by size:
2626 2449 1400 1220 658 135 71 42
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4172 6231 6477 669
Size: 988 281 2234 2059 246 3079
Fragments arranged by size:
3079 2234 2059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2312 2417 6136 748
Size: 1564 105 3719 3499
Fragments arranged by size:
3719 3499 1564 105
HpaI GTT'AAC
Cuts at: 6477 6477
Size: 8887
KpnI G_GTAC'C
Cuts at: 6212 6212
Size: 8887
MfeI C'AATT_G
Cuts at: 5841 6486 5841
Size: 645 8242
MluI A'CGCG_T
Cuts at: 8763 8763
Size: 8887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3325 3332 4149 5673 8001 8185 1305
Size: 2020 7 817 1524 2328 184 2007
Fragments arranged by size:
2328 2020 2007 1524 817 184 7
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3334 5453 5738 10
Size: 54 1425 343 1502 2119 285 3159
Fragments arranged by size:
3159 2119 1502 1425 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2499 2774 4064 6845 7204 7363 518
Size: 648 1333 275 1290 2781 359 159 2042
Fragments arranged by size:
2781 2042 1333 1290 648 359 275 159
MspA1I CmG'CkG
Cuts at: 1606 3835 6303 6980 8128 8373 1606
Size: 2229 2468 677 1148 245 2120
Fragments arranged by size:
2468 2229 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2102 2102
Size: 8887
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7396
Fragments arranged by size:
7396 939 552
NdeI CA'TA_TG
Cuts at: 387 2833 5158 387
Size: 2446 2325 4116
Fragments arranged by size:
4116 2446 2325
NgoMIV G'CCGG_C
Cuts at: 2100 2100
Size: 8887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8887
NotI GC'GGCC_GC
Cuts at: 6334 6334
Size: 8887
NsiI A_TGCA'T
Cuts at: 2833 2833
Size: 8887
NspI r_CATG'y
Cuts at: 1132 1229 2111 2732 2994 3087 4381 6245 7790
Size: 97 882 621 262 93 1294 1864 1545
Cuts at: 7790 1132
Size: 2229
Fragments arranged by size:
2229 1864 1545 1294 882 621 262 97
93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8887
PciI A'CATG_T
Cuts at: 2990 7786 2990
Size: 4796 4091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3835 5449 1108
Size: 2727 1614 4546
Fragments arranged by size:
4546 2727 1614
PfoI T'CCnGG_A
Cuts at: 3891 3891
Size: 8887
PmeI GTTT'AAAC
Cuts at: 6191 6191
Size: 8887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6956 6988 8469 8501 6956
Size: 32 1481 32 7342
Fragments arranged by size:
7342 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 5435 1613
Size: 3822 5065
PsiI TTA'TAA
Cuts at: 3762 5757 6457 3762
Size: 1995 700 6192
Fragments arranged by size:
6192 1995 700
PspOMI G'GGCC_C
Cuts at: 2141 2141
Size: 8887
PspXI vC'TCGA_Gb
Cuts at: 3726 3726
Size: 8887
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2380 2412 2380
Size: 32 8855
PstI C_TGCA'G
Cuts at: 830 3228 3618 6239 830
Size: 2398 390 2621 3478
Fragments arranged by size:
3478 2621 2398 390
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8887
PvuII CAG'CTG
Cuts at: 3835 3835
Size: 8887
SacI G_AGCT'C
Cuts at: 4759 5821 4759
Size: 1062 7825
SalI G'TCGA_C
Cuts at: 1936 4170 6229 1936
Size: 2234 2059 4594
Fragments arranged by size:
4594 2234 2059
SbfI CC_TGCA'GG
Cuts at: 6239 6239
Size: 8887
ScaI AGT'ACT
Cuts at: 7135 8847 7135
Size: 1712 7175
SfcI C'TryA_G
Cuts at: 826 1045 3224 3614 3871 5212 5825 6235 7370
Size: 219 2179 390 257 1341 613 410 1135
Cuts at: 7370 8051 8242 826
Size: 681 191 1471
Fragments arranged by size:
2179 1471 1341 1135 681 613 410 390
257 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8887
SmaI CCC'GGG
Cuts at: 1872 2097 6214 1872
Size: 225 4117 4545
Fragments arranged by size:
4545 4117 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3528 3726 4529 6991 7892 8154
Size: 197 926 1585 198 803 2462 901 262
Cuts at: 8154 820
Size: 1553
Fragments arranged by size:
2462 1585 1553 926 901 803 262 198
197
SnaBI TAC'GTA
Cuts at: 493 2387 6678 493
Size: 1894 4291 2702
Fragments arranged by size:
4291 2702 1894
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8887
SphI G_CATG'C
Cuts at: 1132 2111 2732 3087 6245 1132
Size: 979 621 355 3158 3774
Fragments arranged by size:
3774 3158 979 621 355
SrfI GCCC'GGGC
Cuts at: 2097 2097
Size: 8887
SspI AAT'ATT
Cuts at: 5 52 6811 5
Size: 47 6759 2081
Fragments arranged by size:
6759 2081 47
StuI AGG'CCT
Cuts at: 1517 4263 1517
Size: 2746 6141
StyI C'CwwG_G
Cuts at: 513 1065 2004 2356 3801 4571 6326 513
Size: 552 939 352 1445 770 1755 3074
Fragments arranged by size:
3074 1755 1445 939 770 552 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3748 6914 6931 7084 7269 7714 325
Size: 1255 2168 3166 17 153 185 445 1498
Fragments arranged by size:
3166 2168 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3412 3696 4495
Size: 274 80 33 51 1443 1435 284 799
Cuts at: 4495 4875 7133 8845 96
Size: 380 2258 1712 138
Fragments arranged by size:
2258 1712 1443 1435 799 380 284 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8847 8879 8847
Size: 32 8855
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8887
XbaI T'CTAG_A
Cuts at: 6223 6223
Size: 8887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4382 4382
Size: 8887
XhoI C'TCGA_G
Cuts at: 1943 3726 1943
Size: 1783 7104
XmaI C'CCGG_G
Cuts at: 1870 2095 6212 1870
Size: 225 4117 4545
Fragments arranged by size:
4545 4117 225
XmnI GAAnn'nnTTC
Cuts at: 2763 3306 5276 5484 5934 7016 2763
Size: 543 1970 208 450 1082 4634
Fragments arranged by size:
4634 1970 1082 543 450 208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3350 6695 276
Size: 53 83 186 936 1816 3345 2468
Fragments arranged by size:
3345 2468 1816 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BplI BsiWI
BsmBI BstXI BstZ17I EcoO109I EcoRV FseI FspAI KasI
NarI NruI PacI PmlI PpuMI RsrII SacII SanDI
SapI SexAI SfiI SfoI SwaI