Restriction map for
pFN21AE2686
(Circular) (Six-base) MAPSORT of: pFN21AE2686.seq Check: 4285 from: 1 to: 6229
pFN21AE2686 6229 bp
With 182 enzymes: SgfI *
May 11, 2017 14:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4039 278
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 3132 4356 4729 1563
Size: 1569 1224 373 3063
Fragments arranged by size:
3063 1569 1224 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4292 5676 5944 1632
Size: 2660 1384 268 1917
Fragments arranged by size:
2660 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 5128 6105 1203
Size: 3925 977 1327
Fragments arranged by size:
3925 1327 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 2317 4958 2317
Size: 2641 3588
AleI CACnn'nnGTG
Cuts at: 1166 2930 1166
Size: 1764 4465
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6229
AlwNI CAG_nnn'CTG
Cuts at: 2428 3972 5544 2428
Size: 1544 1572 3113
Fragments arranged by size:
3113 1572 1544
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 2762 3538 3764 3916 6025 1493
Size: 1269 776 226 152 2109 1697
Fragments arranged by size:
2109 1697 1269 776 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 3554 1870
Size: 73 1611 4545
Fragments arranged by size:
4545 1611 73
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2067 2075 2210 3222 3403
Size: 325 217 627 280 8 135 1012 181
Cuts at: 3403 3550 5005 618
Size: 147 1455 1842
Fragments arranged by size:
1842 1455 1012 627 325 280 217 181
147 135 8
BanII G_rGCy'C
Cuts at: 1391 2016 3360 3548 1391
Size: 625 1344 188 4072
Fragments arranged by size:
4072 1344 625 188
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2509 928
Size: 971 610 4648
Fragments arranged by size:
4648 971 610
BbvCI CC'TCA_GC
Cuts at: 3516 3516
Size: 6229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2357 2391 4420 4454 1903
Size: 34 420 34 2029 34 3678
Fragments arranged by size:
3678 2029 420 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BfrBI ATG'CAT
Cuts at: 2152 2152
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4840 136
Size: 107 122 71 631 605 3168 1525
Fragments arranged by size:
3168 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6224 6224
Size: 6229
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2213 3173 4290 5446 1790
Size: 282 141 960 1117 1156 2573
Fragments arranged by size:
2573 1156 1117 960 282 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3960 4918 442
Size: 416 53 83 399 24 2543 958 1753
Fragments arranged by size:
2543 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 3083 3124 3419 3520 4889 1239
Size: 1211 633 41 295 101 1369 2579
Fragments arranged by size:
2579 1369 1211 633 295 101 41
Bpu10I CC'TnA_GC
Cuts at: 2630 3246 3516 2630
Size: 616 270 5343
Fragments arranged by size:
5343 616 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4354 5219 5517 4354
Size: 865 298 5066
Fragments arranged by size:
5066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 4892 882
Size: 617 1413 1980 2219
Fragments arranged by size:
2219 1980 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 2418 6223 2418
Size: 3805 2424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4036 4418 5053 275
Size: 53 83 186 936 2503 382 635 1451
Fragments arranged by size:
2503 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2766 4662 5060 5334 5481 5892 2766
Size: 1896 398 274 147 411 3103
Fragments arranged by size:
3103 1896 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3122 3152 1178
Size: 30 165 30 1719 30 4255
Fragments arranged by size:
4255 1719 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3008 3044 1186
Size: 1822 36 4371
Fragments arranged by size:
4371 1822 36
BseYI C'CCAG_C
Cuts at: 3273 5432 5928 3273
Size: 2159 496 3574
Fragments arranged by size:
3574 2159 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2945 1163
Size: 573 1209 4447
Fragments arranged by size:
4447 1209 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3679 4440 5468 1540
Size: 275 186 1678 761 1028 2301
Fragments arranged by size:
2301 1678 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 3360 4290 4375 5446 2016
Size: 1344 930 85 1071 2799
Fragments arranged by size:
2799 1344 1071 930 85
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
BsmBI CGTCTCn'nnnn_
Cuts at: 3056 3056
Size: 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2072 2213 3173 3360 3548 4290
Size: 399 226 56 141 960 187 188 742
Cuts at: 4290 4375 5446 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 960 742 399 226 188 187
141 85 56
BspEI T'CCGG_A
Cuts at: 2766 5892 2766
Size: 3126 3103
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3584 844
Size: 2740 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4116 1394
Size: 429 2293 3507
Fragments arranged by size:
3507 2293 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2839 4724 4898 65
Size: 1183 1591 1885 174 1396
Fragments arranged by size:
1885 1591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4873 5060 1700
Size: 110 3063 187 2869
Fragments arranged by size:
3063 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 2015 4289 5301 2015
Size: 2274 1012 2943
Fragments arranged by size:
2943 2274 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 2527 1184
Size: 1343 4886
BstXI CCAn_nnnn'nTGG
Cuts at: 2791 2932 2791
Size: 141 6088
BstYI r'GATC_y
Cuts at: 1972 3339 3559 3926 4311 4328 5780 5866 5878
Size: 1367 220 367 385 17 1452 86 12
Cuts at: 5878 6224 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1367 385 367 346 220 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2970 3092 2970
Size: 122 6107
BtgI C'CryG_G
Cuts at: 513 1065 3099 513
Size: 552 2034 3643
Fragments arranged by size:
3643 2034 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2299 2402 487
Size: 683 56 1073 103 4314
Fragments arranged by size:
4314 1073 683 103 56
BtsI GCAGTG_nn'
Cuts at: 3745 4539 4559 3745
Size: 794 20 5415
Fragments arranged by size:
5415 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2233 2247
Size: 54 1425 50 275 18 313 90 14
Cuts at: 2247 2391 2475 3676 4565 6194 8
Size: 144 84 1201 889 1629 43
Fragments arranged by size:
1629 1425 1201 889 313 275 144 90
84 54 50 43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 3676 1537
Size: 275 1864 4090
Fragments arranged by size:
4090 1864 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3365 4159 1353
Size: 2012 794 3423
Fragments arranged by size:
3423 2012 794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2014 3358 2014
Size: 1344 4885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 3083 3124 3419 3520 4292 4889
Size: 393 818 633 41 295 101 772 597
Cuts at: 4889 5676 5944 1239
Size: 787 268 1524
Fragments arranged by size:
1524 818 787 772 633 597 393 295
268 101 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2408 3062 2408
Size: 654 5575
EcoO109I rG'GnC_Cy
Cuts at: 2578 3270 3317 2578
Size: 692 47 5490
Fragments arranged by size:
5490 692 47
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3469 3469
Size: 6229
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 3200 5376 1678
Size: 1522 2176 2531
Fragments arranged by size:
2531 2176 1522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 2428 2460 2837
Size: 32 135 32 731 32 93 32 377
Cuts at: 2837 2869 4870 4902 4944 4976 1373
Size: 32 2001 32 42 32 2626
Fragments arranged by size:
2626 2001 731 377 135 93 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3136 3573 3819 669
Size: 988 281 1198 437 246 3079
Fragments arranged by size:
3079 1198 988 437 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2346 5343 5527 1305
Size: 1041 2997 184 2007
Fragments arranged by size:
2997 2007 1041 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 2393 2477 10
Size: 54 1425 343 417 144 84 3762
Fragments arranged by size:
3762 1425 417 343 144 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2786 2801 2930 3207 4187 4546 4705
Size: 648 1620 15 129 277 980 359 159
Cuts at: 4705 518
Size: 2042
Fragments arranged by size:
2042 1620 980 648 359 277 159 129
15
MspA1I CmG'CkG
Cuts at: 1606 2245 2371 3266 3421 3645 4322 5470 5715
Size: 639 126 895 155 224 677 1148 245
Cuts at: 5715 1606
Size: 2120
Fragments arranged by size:
2120 1148 895 677 639 245 224 155
126
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5677
NdeI CA'TA_TG
Cuts at: 387 2148 387
Size: 1761 4468
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NsiI A_TGCA'T
Cuts at: 2154 2154
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 2791 3587 5132 1132
Size: 97 1562 796 1545 2229
Fragments arranged by size:
2229 1562 1545 796 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 3010 3413 1108
Size: 1137 765 403 3924
Fragments arranged by size:
3924 1137 765 403
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 3270 3317 2578
Size: 692 47 5490
Fragments arranged by size:
5490 692 47
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PstI C_TGCA'G
Cuts at: 830 2175 3387 3581 830
Size: 1345 1212 194 3478
Fragments arranged by size:
3478 1345 1212 194
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2245 2371 3421 2245
Size: 126 1050 5053
Fragments arranged by size:
5053 1050 126
SacI G_AGCT'C
Cuts at: 2016 3360 2016
Size: 1344 4885
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SanDI GG'GwC_CC
Cuts at: 2578 3270 2578
Size: 692 5537
SapI GCTCTTCn'nnn_
Cuts at: 3365 3365
Size: 6229
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SfcI C'TryA_G
Cuts at: 826 1045 2171 2220 3383 3577 4712 5393 5584
Size: 219 1126 49 1163 194 1135 681 191
Cuts at: 5584 826
Size: 1471
Fragments arranged by size:
1471 1163 1135 1126 681 219 194 191
49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 3556 1872
Size: 1684 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4333 5234 5496 820
Size: 197 926 2390 901 262 1553
Fragments arranged by size:
2390 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 3497 1517
Size: 1980 4249
StyI C'CwwG_G
Cuts at: 513 1065 2250 3444 3668 513
Size: 552 1185 1194 224 3074
Fragments arranged by size:
3074 1194 1185 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2495 4256 4273 4426 4611 5056 325
Size: 1255 915 1761 17 153 185 445 1498
Fragments arranged by size:
1761 1498 1255 915 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2456 4475 6187
Size: 274 80 33 51 1443 479 2019 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
2019 1712 1443 479 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6189 6221 6189
Size: 32 6197
Tth111I GACn'n_nGTC
Cuts at: 1316 2654 1316
Size: 1338 4891
XbaI T'CTAG_A
Cuts at: 2308 3565 2308
Size: 1257 4972
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2686 2929 2686
Size: 243 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 3554 1870
Size: 1684 4545
XmnI GAAnn'nnTTC
Cuts at: 2672 4358 2672
Size: 1686 4543
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4037 276
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI
SwaI