Restriction map for
pFN21AE2683
(Circular) (Six-base) MAPSORT of: pFN21AE2683.seq Check: 205 from: 1 to: 7774
pFN21AE2683 7774 bp
With 182 enzymes: SgfI *
March 22, 2017 12:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2180 4014 2180
Size: 1834 5940
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5584 278
Size: 53 83 186 936 4048 2468
Fragments arranged by size:
4048 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5117 1937
Size: 3180 4594
Acc65I G'GTAC_C
Cuts at: 5095 5095
Size: 7774
AclI AA'CG_TT
Cuts at: 1563 5901 6274 1563
Size: 4338 373 3063
Fragments arranged by size:
4338 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2337 4603 4778 5837 7221 7489 1632
Size: 705 2266 175 1059 1384 268 1917
Fragments arranged by size:
2266 1917 1384 1059 705 268 175
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7577
AflIII A'CryG_T
Cuts at: 1203 6673 7650 1203
Size: 5470 977 1327
Fragments arranged by size:
5470 1327 977
AgeI A'CCGG_T
Cuts at: 4848 6605 4848
Size: 1757 6017
AhdI GACnn_n'nnGTC
Cuts at: 6503 6503
Size: 7774
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7774
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2127 2695 1184
Size: 943 568 6263
Fragments arranged by size:
6263 943 568
AlwNI CAG_nnn'CTG
Cuts at: 2213 2447 3953 4230 5068 5517 7089 2213
Size: 234 1506 277 838 449 1572 2898
Fragments arranged by size:
2898 1572 1506 838 449 277 234
ApaI G_GGCC'C
Cuts at: 3219 3467 3219
Size: 248 7526
ApaLI G'TGCA_C
Cuts at: 5831 6987 5831
Size: 1156 6618
ApoI r'AATT_y
Cuts at: 1493 5083 5309 5461 7570 1493
Size: 3590 226 152 2109 1697
Fragments arranged by size:
3590 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6328 160
Size: 872 5296 1606
Fragments arranged by size:
5296 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
AvaI C'yCGr_G
Cuts at: 1870 1943 2767 2796 4749 5099 1870
Size: 73 824 29 1953 350 4545
Fragments arranged by size:
4545 1953 824 350 73 29
BamHI G'GATC_C
Cuts at: 3856 5104 5471 3856
Size: 1248 367 6159
Fragments arranged by size:
6159 1248 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2193 2541 2831 2994 3720
Size: 325 217 627 406 348 290 163 726
Cuts at: 3720 4181 4241 5095 6550 618
Size: 461 60 854 1455 1842
Fragments arranged by size:
1842 1455 854 726 627 461 406 348
325 290 217 163 60
BanII G_rGCy'C
Cuts at: 1391 2046 2250 2292 2463 2774 3219 3419 3467
Size: 655 204 42 171 311 445 200 48
Cuts at: 3467 3510 3647 3798 3996 4361 4523 4748 4914
Size: 43 137 151 198 365 162 225 166
Cuts at: 4914 4941 5093 1391
Size: 27 152 4072
Fragments arranged by size:
4072 655 445 365 311 225 204 200
198 171 166 162 152 151 137 48
43 42 27
BbeI G_GCGC'C
Cuts at: 2545 4245 2545
Size: 1700 6074
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2856 3306 4227 4344 4389 928
Size: 971 957 450 921 117 45 4313
Fragments arranged by size:
4313 971 957 921 450 117 45
BbvCI CC'TCA_GC
Cuts at: 2380 4130 4194 2380
Size: 1750 64 5960
Fragments arranged by size:
5960 1750 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5965 5999 1903
Size: 34 4028 34 3678
Fragments arranged by size:
4028 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5666 6876 5666
Size: 1210 6564
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7774
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2282 2550 3014
Size: 107 122 71 631 605 610 268 464
Cuts at: 3014 4037 4190 6385 136
Size: 1023 153 2195 1525
Fragments arranged by size:
2195 1525 1023 631 610 605 464 268
153 122 107 71
BglII A'GATC_T
Cuts at: 2558 7769 2558
Size: 5211 2563
BlpI GC'TnA_GC
Cuts at: 4925 5191 4925
Size: 266 7508
Bme1580I G_kGCm'C
Cuts at: 1790 2198 2836 3219 3428 3467 4186 5835 6991
Size: 408 638 383 209 39 719 1649 1156
Cuts at: 6991 1790
Size: 2573
Fragments arranged by size:
2573 1649 1156 719 638 408 383 209
39
BmgBI CAC'GTC
Cuts at: 1318 2326 3055 1318
Size: 1008 729 6037
Fragments arranged by size:
6037 1008 729
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5505 6463 442
Size: 416 53 83 399 24 4088 958 1753
Fragments arranged by size:
4088 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7774
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2449 3946 2065
Size: 384 1497 5893
Fragments arranged by size:
5893 1497 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2699 2769 3266 3431 3659 4337 4349 4717
Size: 1460 70 497 165 228 678 12 368
Cuts at: 4717 6434 1239
Size: 1717 2579
Fragments arranged by size:
2579 1717 1460 678 497 368 228 165
70 12
Bpu10I CC'TnA_GC
Cuts at: 2380 2931 3456 4130 4194 2380
Size: 551 525 674 64 5960
Fragments arranged by size:
5960 674 551 525 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3033 3920 4181 4549 5899 6764 7062 3033
Size: 887 261 368 1350 865 298 3745
Fragments arranged by size:
3745 1350 887 865 368 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4068 4266 4313 6437 882
Size: 617 2569 198 47 2124 2219
Fragments arranged by size:
2569 2219 2124 617 198 47
BsaAI yAC'GTr
Cuts at: 493 1204 5565 493
Size: 711 4361 2702
Fragments arranged by size:
4361 2702 711
BsaBI GATnn'nnATC
Cuts at: 7768 7768
Size: 7774
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2542 4110 4242 5581
Size: 53 83 186 936 1009 1568 132 1339
Cuts at: 5581 5963 6598 275
Size: 382 635 1451
Fragments arranged by size:
1568 1451 1339 1009 936 635 382 186
132 83 53
BsaWI w'CCGG_w
Cuts at: 4848 6207 6605 6879 7026 7437 4848
Size: 1359 398 274 147 411 5185
Fragments arranged by size:
5185 1359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 2697 2727 3338
Size: 30 165 30 771 30 493 30 611
Cuts at: 3338 3368 4331 4361 4730 4760 4898 4928 1178
Size: 30 963 30 369 30 138 30 4024
Fragments arranged by size:
4024 963 771 611 493 369 165 138
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2114 2471 3194 3308 3329 3332 3356
Size: 852 76 357 723 114 21 3 24
Cuts at: 3356 3359 3362 3365 3866 4346 4439 4460 4463
Size: 3 3 3 501 480 93 21 3
Cuts at: 4463 4466 4469 4805 1186
Size: 3 3 336 4155
Fragments arranged by size:
4155 852 723 501 480 357 336 114
93 76 24 21 21 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2139 2344 2411 2440 2775 2964 3397 3460 3568
Size: 205 67 29 335 189 433 63 108
Cuts at: 3568 3683 4023 4173 5058 6977 7473 2139
Size: 115 340 150 885 1919 496 2440
Fragments arranged by size:
2440 1919 885 496 433 340 335 205
189 150 115 108 67 63 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2388 1163
Size: 573 652 6549
Fragments arranged by size:
6549 652 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5224 5985 7013 1540
Size: 275 186 3223 761 1028 2301
Fragments arranged by size:
3223 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2250 2292 2463 3060 3212 3419 3520 3798
Size: 204 42 171 597 152 207 101 278
Cuts at: 3798 3996 4098 4748 4863 5835 5920 6991 2046
Size: 198 102 650 115 972 85 1071 2829
Fragments arranged by size:
2829 1071 972 650 597 278 207 204
198 171 152 115 102 101 85 42
BsmI GAATG_Cn'
Cuts at: 5285 5378 5285
Size: 93 7681
BsmBI CGTCTCn'nnnn_
Cuts at: 2355 2355
Size: 7774
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2198 2250 2292 2463 2774 2836
Size: 399 256 152 52 42 171 311 62
Cuts at: 2836 3060 3212 3219 3419 3428 3467 3510 3520
Size: 224 152 7 200 9 39 43 10
Cuts at: 3520 3647 3798 3996 4098 4186 4361 4523 4748
Size: 127 151 198 102 88 175 162 225
Cuts at: 4748 4863 4914 4941 5093 5835 5920 6991 1391
Size: 115 51 27 152 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 311 256 225 224
200 198 175 171 162 152 152 152
151 127 115 102 88 85 62 52
51 43 42 39 27 10 9 7
BspEI T'CCGG_A
Cuts at: 7437 7437
Size: 7774
BspHI T'CATG_A
Cuts at: 5663 7393 5663
Size: 1730 6044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2180 2959 4014 5129 844
Size: 1336 779 1055 1115 3489
Fragments arranged by size:
3489 1336 1115 1055 779
BsrBI CCG'CTC
Cuts at: 1394 1823 3562 4563 5661 1394
Size: 429 1739 1001 1098 3507
Fragments arranged by size:
3507 1739 1098 1001 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6269 6443 65
Size: 1183 5021 174 1396
Fragments arranged by size:
5021 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3267 4539 4848 6418 6605 1700
Size: 110 1457 1272 309 1570 187 2869
Fragments arranged by size:
2869 1570 1457 1272 309 187 110
BsrGI T'GTAC_A
Cuts at: 96 2642 4716 96
Size: 2546 2074 3154
Fragments arranged by size:
3154 2546 2074
BssHII G'CGCG_C
Cuts at: 1925 2025 1925
Size: 100 7674
BssSI C'ACGA_G
Cuts at: 5834 6846 5834
Size: 1012 6762
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5068 794
Size: 4274 3500
BstBI TT'CG_AA
Cuts at: 6578 6578
Size: 7774
BstEII G'GTnAC_C
Cuts at: 1184 4596 1184
Size: 3412 4362
BstXI CCAn_nnnn'nTGG
Cuts at: 2302 2302
Size: 7774
BstYI r'GATC_y
Cuts at: 1972 2558 2975 3627 3856 4281 5104 5471 5856
Size: 586 417 652 229 425 823 367 385
Cuts at: 5856 5873 7325 7411 7423 7769 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 823 652 586 425 417 385
367 346 229 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2448 3921 4895 2448
Size: 1473 974 5327
Fragments arranged by size:
5327 1473 974
BtgI C'CryG_G
Cuts at: 513 1065 2004 3386 3579 5028 513
Size: 552 939 1382 193 1449 3259
Fragments arranged by size:
3259 1449 1382 939 552 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2876 487
Size: 683 56 1650 5385
Fragments arranged by size:
5385 1650 683 56
BtsI GCAGTG_nn'
Cuts at: 2195 2465 3236 4504 5290 6084 6104 2195
Size: 270 771 1268 786 794 20 3865
Fragments arranged by size:
3865 1268 794 786 771 270 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7774
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2158 2193 529
Size: 35 1594 35 6110
Fragments arranged by size:
6110 1594 35 35
DraI TTT'AAA
Cuts at: 5078 5078
Size: 7774
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4481 6781 809
Size: 507 3165 2300 1802
Fragments arranged by size:
3165 2300 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2487 3087
Size: 54 1425 50 275 18 336 321 600
Cuts at: 3087 3595 4042 5221 6110 7739 8
Size: 508 447 1179 889 1629 43
Fragments arranged by size:
1629 1425 1179 889 600 508 447 336
321 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5221 1537
Size: 275 3409 4090
Fragments arranged by size:
4090 3409 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 2255 3306 3318 3761 4425 4464 4582
Size: 681 221 1051 12 443 664 39 118
Cuts at: 4582 4754 4983 5704 1353
Size: 172 229 721 3423
Fragments arranged by size:
3423 1051 721 681 664 443 229 221
172 118 39 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2603 3254 6361 6735 6881 387
Size: 1064 138 1014 651 3107 374 146 1280
Fragments arranged by size:
3107 1280 1064 1014 651 374 146 138
EcoICRI GAG'CTC
Cuts at: 2044 2248 2290 2461 3417 3796 3994 4746 2044
Size: 204 42 171 956 379 198 752 5072
Fragments arranged by size:
5072 956 752 379 204 198 171 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2337 2699 2769 3266 3431 3659 4337
Size: 393 705 362 70 497 165 228 678
Cuts at: 4337 4349 4603 4717 4778 5837 6434 7221 7489
Size: 12 254 114 61 1059 597 787 268
Cuts at: 7489 1239
Size: 1524
Fragments arranged by size:
1524 1059 787 705 678 597 497 393
362 268 254 228 165 114 70 61
12
EcoNI CCTnn'n_nnAGG
Cuts at: 4331 4421 5004 4331
Size: 90 583 7101
Fragments arranged by size:
7101 583 90
EcoO109I rG'GnC_Cy
Cuts at: 2275 3132 3464 3620 3679 3925 4134 4204 4993
Size: 857 332 156 59 246 209 70 789
Cuts at: 4993 2275
Size: 5056
Fragments arranged by size:
5056 857 789 332 246 209 156 70
59
EcoRI G'AATT_C
Cuts at: 5083 5461 5083
Size: 378 7396
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3787 4072 4783 4852 5012 3787
Size: 285 711 69 160 6549
Fragments arranged by size:
6549 711 285 160 69
FspI TGC'GCA
Cuts at: 6280 6280
Size: 7774
HaeII r_GCGC'y
Cuts at: 1678 2545 4245 4768 4890 6921 1678
Size: 867 1700 523 122 2031 2531
Fragments arranged by size:
2531 2031 1700 867 523 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2354 2386 2447
Size: 32 135 32 575 32 175 32 61
Cuts at: 2447 2479 2611 2643 3612 3644 3983 4015 4331
Size: 32 132 32 969 32 339 32 316
Cuts at: 4331 4363 4730 4762 6415 6447 6489 6521 1373
Size: 32 367 32 1653 32 42 32 2626
Fragments arranged by size:
2626 1653 969 575 367 339 316 175
135 132 61 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2570 5118 5364 669
Size: 988 281 632 2548 246 3079
Fragments arranged by size:
3079 2548 988 632 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7774
HpaI GTT'AAC
Cuts at: 5364 5364
Size: 7774
KasI G'GCGC_C
Cuts at: 2541 4241 2541
Size: 1700 6074
KpnI G_GTAC'C
Cuts at: 5099 5099
Size: 7774
MfeI C'AATT_G
Cuts at: 5373 5373
Size: 7774
MluI A'CGCG_T
Cuts at: 7650 7650
Size: 7774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4123 6888 7072 1305
Size: 2818 2765 184 2007
Fragments arranged by size:
2818 2765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2489 3089 3597 4044 10
Size: 54 1425 343 657 600 508 447 3740
Fragments arranged by size:
3740 1425 657 600 508 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5732 6091 6250 518
Size: 648 4566 359 159 2042
Fragments arranged by size:
4566 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 3493 3687 4401 4924 5190 5867 7015
Size: 537 1350 194 714 523 266 677 1148
Cuts at: 7015 7260 1606
Size: 245 2120
Fragments arranged by size:
2120 1350 1148 714 677 537 523 266
245 194
NaeI GCC'GGC
Cuts at: 3269 3269
Size: 7774
NarI GG'CG_CC
Cuts at: 2542 4242 2542
Size: 1700 6074
NcoI C'CATG_G
Cuts at: 513 1065 2004 3386 5028 513
Size: 552 939 1382 1642 3259
Fragments arranged by size:
3259 1642 1382 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7774
NgoMIV G'CCGG_C
Cuts at: 3267 3267
Size: 7774
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7774
NotI GC'GGCC_GC
Cuts at: 5221 5221
Size: 7774
NspI r_CATG'y
Cuts at: 1132 1229 2857 2887 4016 5075 5132 6677 1132
Size: 97 1628 30 1129 1059 57 1545 2229
Fragments arranged by size:
2229 1628 1545 1129 1059 97 57 30
PasI CC'CwG_GG
Cuts at: 1348 3947 1348
Size: 2599 5175
PciI A'CATG_T
Cuts at: 6673 6673
Size: 7774
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3758 1108
Size: 2650 5124
PmeI GTTT'AAAC
Cuts at: 5078 5078
Size: 7774
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2118 2150 5843 5875 7356 7388 2118
Size: 32 3693 32 1481 32 2504
Fragments arranged by size:
3693 2504 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3620 3679 4134 3620
Size: 59 455 7260
Fragments arranged by size:
7260 455 59
PshAI GACnn'nnGTC
Cuts at: 1613 2328 1613
Size: 715 7059
PsiI TTA'TAA
Cuts at: 5344 5344
Size: 7774
PspOMI G'GGCC_C
Cuts at: 3215 3463 3215
Size: 248 7526
PstI C_TGCA'G
Cuts at: 830 2190 2475 2688 2703 2954 3285 5126 830
Size: 1360 285 213 15 251 331 1841 3478
Fragments arranged by size:
3478 1841 1360 331 285 251 213 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7774
PvuII CAG'CTG
Cuts at: 2143 3687 4924 2143
Size: 1544 1237 4993
Fragments arranged by size:
4993 1544 1237
SacI G_AGCT'C
Cuts at: 2046 2250 2292 2463 3419 3798 3996 4748 2046
Size: 204 42 171 956 379 198 752 5072
Fragments arranged by size:
5072 956 752 379 204 198 171 42
SalI G'TCGA_C
Cuts at: 1936 5116 1936
Size: 3180 4594
SanDI GG'GwC_CC
Cuts at: 3620 3620
Size: 7774
SapI GCTCTTCn'nnn_
Cuts at: 2034 2255 3761 4582 2034
Size: 221 1506 821 5226
Fragments arranged by size:
5226 1506 821 221
SbfI CC_TGCA'GG
Cuts at: 2688 5126 2688
Size: 2438 5336
ScaI AGT'ACT
Cuts at: 6022 7734 6022
Size: 1712 6062
SfcI C'TryA_G
Cuts at: 826 1045 2186 2471 2684 2699 2950 3281 5122
Size: 219 1141 285 213 15 251 331 1841
Cuts at: 5122 6257 6938 7129 826
Size: 1135 681 191 1471
Fragments arranged by size:
1841 1471 1141 1135 681 331 285 251
219 213 191 15
SfoI GGC'GCC
Cuts at: 2543 4243 2543
Size: 1700 6074
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7774
SmaI CCC'GGG
Cuts at: 1872 2798 5101 1872
Size: 926 2303 4545
Fragments arranged by size:
4545 2303 926
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3048 3935 4160 4564 5878 6779
Size: 197 926 1105 887 225 404 1314 901
Cuts at: 6779 7041 820
Size: 262 1553
Fragments arranged by size:
1553 1314 1105 926 901 887 404 262
225 197
SnaBI TAC'GTA
Cuts at: 493 5565 493
Size: 5072 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7774
SphI G_CATG'C
Cuts at: 1132 5132 1132
Size: 4000 3774
SspI AAT'ATT
Cuts at: 5 52 5698 5
Size: 47 5646 2081
Fragments arranged by size:
5646 2081 47
StuI AGG'CCT
Cuts at: 1517 2553 2948 3152 3381 3548 1517
Size: 1036 395 204 229 167 5743
Fragments arranged by size:
5743 1036 395 229 204 167
StyI C'CwwG_G
Cuts at: 513 1065 2004 2736 2943 3386 3511 3543 4187
Size: 552 939 732 207 443 125 32 644
Cuts at: 4187 4251 5028 5213 513
Size: 64 777 185 3074
Fragments arranged by size:
3074 939 777 732 644 552 443 207
185 125 64 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2534 2612 3108 3217 3648 3838 3993
Size: 1255 954 78 496 109 431 190 155
Cuts at: 3993 4264 5801 5818 5971 6156 6601 325
Size: 271 1537 17 153 185 445 1498
Fragments arranged by size:
1537 1498 1255 954 496 445 431 271
190 185 155 153 109 78 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2642 4716
Size: 274 80 33 51 1443 249 416 2074
Cuts at: 4716 6020 7732 96
Size: 1304 1712 138
Fragments arranged by size:
2074 1712 1443 1304 416 274 249 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 2697 2729 3338 3370 7734 7766 2174
Size: 32 491 32 609 32 4364 32 2182
Fragments arranged by size:
4364 2182 609 491 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4373 1316
Size: 3057 4717
XbaI T'CTAG_A
Cuts at: 5110 5110
Size: 7774
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7774
XmaI C'CCGG_G
Cuts at: 1870 2796 5099 1870
Size: 926 2303 4545
Fragments arranged by size:
4545 2303 926
XmnI GAAnn'nnTTC
Cuts at: 5903 5903
Size: 7774
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5582 276
Size: 53 83 186 936 4048 2468
Fragments arranged by size:
4048 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI
SwaI XcmI