Restriction map for
pFN21AE2682
(Circular) (Six-base) MAPSORT of: pFN21AE2682.seq Check: 3719 from: 1 to: 6682
pFN21AE2682 6682 bp
With 182 enzymes: SgfI *
March 10, 2017 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4492 278
Size: 53 83 186 936 2956 2468
Fragments arranged by size:
2956 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4025 1937
Size: 2088 4594
Acc65I G'GTAC_C
Cuts at: 3707 4003 3707
Size: 296 6386
AclI AA'CG_TT
Cuts at: 1563 3958 4809 5182 1563
Size: 2395 851 373 3063
Fragments arranged by size:
3063 2395 851 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 4745 6129 6397 1632
Size: 470 2643 1384 268 1917
Fragments arranged by size:
2643 1917 1384 470 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6485
AflIII A'CryG_T
Cuts at: 1203 3372 5581 6558 1203
Size: 2169 2209 977 1327
Fragments arranged by size:
2209 2169 1327 977
AgeI A'CCGG_T
Cuts at: 5513 5513
Size: 6682
AhdI GACnn_n'nnGTC
Cuts at: 5411 5411
Size: 6682
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6682
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6682
AlwNI CAG_nnn'CTG
Cuts at: 2449 4425 5997 2449
Size: 1976 1572 3134
Fragments arranged by size:
3134 1976 1572
ApaI G_GGCC'C
Cuts at: 3359 3359
Size: 6682
ApaLI G'TGCA_C
Cuts at: 4739 5895 4739
Size: 1156 5526
ApoI r'AATT_y
Cuts at: 1493 2180 3337 3736 3991 4217 4369 6478 1493
Size: 687 1157 399 255 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 687 399 255 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5236 160
Size: 872 4204 1606
Fragments arranged by size:
4204 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
AvaI C'yCGr_G
Cuts at: 1870 1943 2619 3622 4007 1870
Size: 73 676 1003 385 4545
Fragments arranged by size:
4545 1003 676 385 73
AvrII C'CTAG_G
Cuts at: 2707 2707
Size: 6682
BamHI G'GATC_C
Cuts at: 4012 4379 4012
Size: 367 6315
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3164 3208 3707 4003 5458
Size: 325 217 627 1377 44 499 296 1455
Cuts at: 5458 618
Size: 1842
Fragments arranged by size:
1842 1455 1377 627 499 325 296 217
44
BanII G_rGCy'C
Cuts at: 1391 2973 3192 3359 4001 1391
Size: 1582 219 167 642 4072
Fragments arranged by size:
4072 1582 642 219 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2016 2351 3180 3302 928
Size: 971 117 335 829 122 4308
Fragments arranged by size:
4308 971 829 335 122 117
BbvCI CC'TCA_GC
Cuts at: 3974 3974
Size: 6682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2685 2719 4873 4907 1903
Size: 34 748 34 2154 34 3678
Fragments arranged by size:
3678 2154 748 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2437 2723 4574 5784 2437
Size: 286 1851 1210 3335
Fragments arranged by size:
3335 1851 1210 286
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6682
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5293 136
Size: 107 122 71 631 605 3621 1525
Fragments arranged by size:
3621 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2113 2187 3787 6677 2113
Size: 74 1600 2890 2118
Fragments arranged by size:
2890 2118 1600 74
BlpI GC'TnA_GC
Cuts at: 4099 4099
Size: 6682
Bme1580I G_kGCm'C
Cuts at: 1790 2304 3169 3359 3826 4743 5899 1790
Size: 514 865 190 467 917 1156 2573
Fragments arranged by size:
2573 1156 917 865 514 467 190
BmgBI CAC'GTC
Cuts at: 1318 2678 1318
Size: 1360 5322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4413 5371 442
Size: 416 53 83 399 24 2996 958 1753
Fragments arranged by size:
2996 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2790 3206 2790
Size: 416 6266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2391 2442 2796 3240 5342 1239
Size: 1152 51 354 444 2102 2579
Fragments arranged by size:
2579 2102 1152 444 354 51
Bpu10I CC'TnA_GC
Cuts at: 3974 3974
Size: 6682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2760 3860 4807 5672 5970 2760
Size: 1100 947 865 298 3472
Fragments arranged by size:
3472 1100 947 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3015 5345 882
Size: 617 1516 2330 2219
Fragments arranged by size:
2330 2219 1516 617
BsaAI yAC'GTr
Cuts at: 493 1204 4473 493
Size: 711 3269 2702
Fragments arranged by size:
3269 2702 711
BsaBI GATnn'nnATC
Cuts at: 6676 6676
Size: 6682
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2295 4489 4871 5506
Size: 53 83 186 936 762 2194 382 635
Cuts at: 5506 275
Size: 1451
Fragments arranged by size:
2194 1451 936 762 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2429 3329 5115 5513 5787 5934 6345 2429
Size: 900 1786 398 274 147 411 2766
Fragments arranged by size:
2766 1786 900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6457
Fragments arranged by size:
6457 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3056 3200 1186
Size: 1870 144 4668
Fragments arranged by size:
4668 1870 144
BseYI C'CCAG_C
Cuts at: 2369 2959 3007 3260 5885 6381 2369
Size: 590 48 253 2625 496 2670
Fragments arranged by size:
2670 2625 590 496 253 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2776 2886 1163
Size: 573 1040 110 4959
Fragments arranged by size:
4959 1040 573 110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2695 4132 4893 5921 1540
Size: 275 186 694 1437 761 1028 2301
Fragments arranged by size:
2301 1437 1028 761 694 275 186
BsiHKAI G_wGCw'C
Cuts at: 3192 4743 4828 5899 3192
Size: 1551 85 1071 3975
Fragments arranged by size:
3975 1551 1071 85
BsmI GAATG_Cn'
Cuts at: 4193 4286 4193
Size: 93 6589
BsmBI CGTCTCn'nnnn_
Cuts at: 2668 2668
Size: 6682
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2973 3169 3192 3359 3826 4001
Size: 399 514 669 196 23 167 467 175
Cuts at: 4001 4743 4828 5899 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 514 467 399 196
175 167 85 23
BspEI T'CCGG_A
Cuts at: 2429 3329 6345 2429
Size: 900 3016 2766
Fragments arranged by size:
3016 2766 900
BspHI T'CATG_A
Cuts at: 4571 6301 4571
Size: 1730 4952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 2591 3553 4037 844
Size: 1547 200 962 484 3489
Fragments arranged by size:
3489 1547 962 484 200
BsrBI CCG'CTC
Cuts at: 1394 1823 4569 1394
Size: 429 2746 3507
Fragments arranged by size:
3507 2746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2227 2865 5177 5351 65
Size: 1183 979 638 2312 174 1396
Fragments arranged by size:
2312 1396 1183 979 638 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5326 5513 1700
Size: 110 3516 187 2869
Fragments arranged by size:
3516 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6682
BssSI C'ACGA_G
Cuts at: 2307 4742 5754 2307
Size: 2435 1012 3235
Fragments arranged by size:
3235 2435 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2864 794
Size: 2070 4612
BstBI TT'CG_AA
Cuts at: 5486 5486
Size: 6682
BstEII G'GTnAC_C
Cuts at: 1184 3755 1184
Size: 2571 4111
BstYI r'GATC_y
Cuts at: 1972 2020 2113 2187 3028 3787 4012 4379 4764
Size: 48 93 74 841 759 225 367 385
Cuts at: 4764 4781 6233 6319 6331 6677 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 841 759 385 367 346 225
93 86 74 48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3195 513
Size: 552 939 1191 4000
Fragments arranged by size:
4000 1191 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5943
Fragments arranged by size:
5943 683 56
BtsI GCAGTG_nn'
Cuts at: 4198 4992 5012 4198
Size: 794 20 5868
Fragments arranged by size:
5868 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6682
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6647
DraI TTT'AAA
Cuts at: 3986 3986
Size: 6682
DraIII CAC_nnn'GTG
Cuts at: 3440 3440
Size: 6682
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5689 809
Size: 507 4373 1802
Fragments arranged by size:
4373 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4129 5018 6647
Size: 54 1425 50 275 18 2299 889 1629
Cuts at: 6647 8
Size: 43
Fragments arranged by size:
2299 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4129 1537
Size: 275 2317 4090
Fragments arranged by size:
4090 2317 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3845 4612 1353
Size: 2492 767 3423
Fragments arranged by size:
3423 2492 767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5269 5643 5789 387
Size: 1064 138 3680 374 146 1280
Fragments arranged by size:
3680 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3190 3190
Size: 6682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2391 2442 2796 3240 4745 5342
Size: 393 470 289 51 354 444 1505 597
Cuts at: 5342 6129 6397 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1505 787 597 470 444 393 354
289 268 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3038 3830 3038
Size: 792 5890
EcoO109I rG'GnC_Cy
Cuts at: 2623 3265 3355 3356 2623
Size: 642 90 1 5949
Fragments arranged by size:
5949 642 90 1
EcoRI G'AATT_C
Cuts at: 2180 3991 4369 2180
Size: 1811 378 4493
Fragments arranged by size:
4493 1811 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2132 2132
Size: 6682
FspI TGC'GCA
Cuts at: 5188 5188
Size: 6682
HaeII r_GCGC'y
Cuts at: 1678 5829 1678
Size: 4151 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 2711 2743 2918
Size: 32 135 32 830 32 277 32 175
Cuts at: 2918 2950 3020 3052 3529 3561 5323 5355 5397
Size: 32 70 32 477 32 1762 32 42
Cuts at: 5397 5429 1373
Size: 32 2626
Fragments arranged by size:
2626 1762 830 477 277 175 135 70
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4026 4272 669
Size: 988 281 2088 246 3079
Fragments arranged by size:
3079 2088 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2778 3810 3903 748
Size: 2030 1032 93 3527
Fragments arranged by size:
3527 2030 1032 93
HpaI GTT'AAC
Cuts at: 4272 4272
Size: 6682
KpnI G_GTAC'C
Cuts at: 3711 4007 3711
Size: 296 6386
MfeI C'AATT_G
Cuts at: 2364 3747 4281 2364
Size: 1383 534 4765
Fragments arranged by size:
4765 1383 534
MluI A'CGCG_T
Cuts at: 6558 6558
Size: 6682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2217 3127 5796 5980 1305
Size: 912 910 2669 184 2007
Fragments arranged by size:
2669 2007 912 910 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4860
Fragments arranged by size:
4860 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 4640 4999 5158 518
Size: 648 1095 2379 359 159 2042
Fragments arranged by size:
2379 2042 1095 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3260 4098 4775 5923 6168 1606
Size: 1654 838 677 1148 245 2120
Fragments arranged by size:
2120 1654 1148 838 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3195 513
Size: 552 939 1191 4000
Fragments arranged by size:
4000 1191 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6682
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6682
NotI GC'GGCC_GC
Cuts at: 4129 4129
Size: 6682
NspI r_CATG'y
Cuts at: 1132 1229 2266 2363 3376 4040 5585 1132
Size: 97 1037 97 1013 664 1545 2229
Fragments arranged by size:
2229 1545 1037 1013 664 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6682
PciI A'CATG_T
Cuts at: 3372 5581 3372
Size: 2209 4473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2290 2339 1108
Size: 1182 49 5451
Fragments arranged by size:
5451 1182 49
PfoI T'CCnGG_A
Cuts at: 2946 2946
Size: 6682
PmeI GTTT'AAAC
Cuts at: 3986 3986
Size: 6682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4751 4783 6264 6296 4751
Size: 32 1481 32 5137
Fragments arranged by size:
5137 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2623 3265 2623
Size: 642 6040
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6682
PsiI TTA'TAA
Cuts at: 4252 4252
Size: 6682
PspOMI G'GGCC_C
Cuts at: 3355 3355
Size: 6682
PstI C_TGCA'G
Cuts at: 830 3670 4034 830
Size: 2840 364 3478
Fragments arranged by size:
3478 2840 364
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6682
PvuII CAG'CTG
Cuts at: 3260 3260
Size: 6682
SacI G_AGCT'C
Cuts at: 3192 3192
Size: 6682
SalI G'TCGA_C
Cuts at: 1936 4024 1936
Size: 2088 4594
SbfI CC_TGCA'GG
Cuts at: 4034 4034
Size: 6682
ScaI AGT'ACT
Cuts at: 4930 6642 4930
Size: 1712 4970
SfcI C'TryA_G
Cuts at: 826 1045 2613 2891 3666 4030 5165 5846 6037
Size: 219 1568 278 775 364 1135 681 191
Cuts at: 6037 826
Size: 1471
Fragments arranged by size:
1568 1471 1135 775 681 364 278 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6682
SmaI CCC'GGG
Cuts at: 1872 2621 3624 4009 1872
Size: 749 1003 385 4545
Fragments arranged by size:
4545 1003 749 385
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2775 3875 4786 5687 5949 820
Size: 197 926 832 1100 911 901 262 1553
Fragments arranged by size:
1553 1100 926 911 901 832 262 197
SnaBI TAC'GTA
Cuts at: 493 4473 493
Size: 3980 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6682
SphI G_CATG'C
Cuts at: 1132 4040 1132
Size: 2908 3774
SrfI GCCC'GGGC
Cuts at: 3624 3624
Size: 6682
SspI AAT'ATT
Cuts at: 5 52 3204 4606 5
Size: 47 3152 1402 2081
Fragments arranged by size:
3152 2081 1402 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6682
StyI C'CwwG_G
Cuts at: 513 1065 2004 2707 3195 4121 513
Size: 552 939 703 488 926 3074
Fragments arranged by size:
3074 939 926 703 552 488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2709 3504 4709 4726 4879 5064 5509
Size: 1255 1129 795 1205 17 153 185 445
Cuts at: 5509 325
Size: 1498
Fragments arranged by size:
1498 1255 1205 1129 795 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4928 6640 96
Size: 274 80 33 51 1443 2951 1712 138
Fragments arranged by size:
2951 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6642 6674 6642
Size: 32 6650
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6682
XbaI T'CTAG_A
Cuts at: 4018 4018
Size: 6682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 3202 3718 2533
Size: 669 516 5497
Fragments arranged by size:
5497 669 516
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6682
XmaI C'CCGG_G
Cuts at: 1870 2619 3622 4007 1870
Size: 749 1003 385 4545
Fragments arranged by size:
4545 1003 749 385
XmnI GAAnn'nnTTC
Cuts at: 2115 3077 4811 2115
Size: 962 1734 3986
Fragments arranged by size:
3986 1734 962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4490 276
Size: 53 83 186 936 2956 2468
Fragments arranged by size:
2956 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI