Restriction map for
pFN21AE2678
(Circular) (Six-base) MAPSORT of: pFN21AE2678.seq Check: 9641 from: 1 to: 9157
pFN21AE2678 9157 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5825 6967 278
Size: 53 83 186 936 4289 1142 2468
Fragments arranged by size:
4289 2468 1142 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3573 5451 6500 1937
Size: 1636 1878 1049 4594
Fragments arranged by size:
4594 1878 1636 1049
Acc65I G'GTAC_C
Cuts at: 2197 5581 6478 2197
Size: 3384 897 4876
Fragments arranged by size:
4876 3384 897
AclI AA'CG_TT
Cuts at: 1563 4910 7284 7657 1563
Size: 3347 2374 373 3063
Fragments arranged by size:
3347 3063 2374 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3040 3219 3727 4864 4949 5291 5356 6205
Size: 1408 179 508 1137 85 342 65 849
Cuts at: 6205 7220 8604 8872 1632
Size: 1015 1384 268 1917
Fragments arranged by size:
1917 1408 1384 1137 1015 849 508 342
268 179 85 65
AfeI AGC'GCT
Cuts at: 3670 5366 3670
Size: 1696 7461
AflII C'TTAA_G
Cuts at: 820 1017 3398 820
Size: 197 2381 6579
Fragments arranged by size:
6579 2381 197
AflIII A'CryG_T
Cuts at: 1203 2622 2766 4473 4941 6018 8056 9033 1203
Size: 1419 144 1707 468 1077 2038 977 1327
Fragments arranged by size:
2038 1707 1419 1327 1077 977 468 144
AgeI A'CCGG_T
Cuts at: 5628 7988 5628
Size: 2360 6797
AhdI GACnn_n'nnGTC
Cuts at: 2926 7886 2926
Size: 4960 4197
AleI CACnn'nnGTG
Cuts at: 1166 5687 5924 1166
Size: 4521 237 4399
Fragments arranged by size:
4521 4399 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9157
AlwNI CAG_nnn'CTG
Cuts at: 2813 3197 5791 6900 8472 2813
Size: 384 2594 1109 1572 3498
Fragments arranged by size:
3498 2594 1572 1109 384
ApaI G_GGCC'C
Cuts at: 5520 6274 5520
Size: 754 8403
ApaLI G'TGCA_C
Cuts at: 7214 8370 7214
Size: 1156 8001
ApoI r'AATT_y
Cuts at: 1493 3046 3405 5126 6466 6692 6844 8953 1493
Size: 1553 359 1721 1340 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 1553 1340 359 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7711 160
Size: 872 6679 1606
Fragments arranged by size:
6679 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9157
AvaI C'yCGr_G
Cuts at: 1870 1943 2202 5704 6482 1870
Size: 73 259 3502 778 4545
Fragments arranged by size:
4545 3502 778 259 73
BamHI G'GATC_C
Cuts at: 6487 6854 6487
Size: 367 8790
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2197 3244 3765 5581 6478
Size: 325 217 627 410 1047 521 1816 897
Cuts at: 6478 7933 618
Size: 1455 1842
Fragments arranged by size:
1842 1816 1455 1047 897 627 521 410
325 217
BanII G_rGCy'C
Cuts at: 1391 2083 2099 2393 2835 2955 3539 5013 5520
Size: 692 16 294 442 120 584 1474 507
Cuts at: 5520 5858 6008 6274 6476 1391
Size: 338 150 266 202 4072
Fragments arranged by size:
4072 1474 692 584 507 442 338 294
266 202 150 120 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4459 4579 928
Size: 971 2560 120 5506
Fragments arranged by size:
5506 2560 971 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5213 5247 7348 7382 1903
Size: 34 3276 34 2101 34 3678
Fragments arranged by size:
3678 3276 2101 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2791 7049 8259 2791
Size: 4258 1210 3689
Fragments arranged by size:
4258 3689 1210
BclI T'GATC_A
Cuts at: 1548 3021 1548
Size: 1473 7684
BfrBI ATG'CAT
Cuts at: 4111 4154 4111
Size: 43 9114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 3527 5813
Size: 107 122 71 631 605 388 1467 2286
Cuts at: 5813 5954 7768 136
Size: 141 1814 1525
Fragments arranged by size:
2286 1814 1525 1467 631 605 388 141
122 107 71
BglII A'GATC_T
Cuts at: 9152 9152
Size: 9157
BlpI GC'TnA_GC
Cuts at: 6122 6574 6122
Size: 452 8705
Bme1580I G_kGCm'C
Cuts at: 1790 2135 2781 3588 4065 5520 6075 6274 7218
Size: 345 646 807 477 1455 555 199 944
Cuts at: 7218 8374 1790
Size: 1156 2573
Fragments arranged by size:
2573 1455 1156 944 807 646 555 477
345 199
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9157
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2775 2838 5038
Size: 416 53 83 399 24 1358 63 2200
Cuts at: 5038 5449 5804 6888 7846 442
Size: 411 355 1084 958 1753
Fragments arranged by size:
2200 1753 1358 1084 958 416 411 399
355 83 63 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9157
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5794 5794
Size: 9157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3257 6391 7817 1239
Size: 2018 3134 1426 2579
Fragments arranged by size:
3134 2579 2018 1426
Bpu10I CC'TnA_GC
Cuts at: 2949 4001 2949
Size: 1052 8105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2648 7282 8147 8445 2648
Size: 4634 865 298 3360
Fragments arranged by size:
4634 3360 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2222 4015 5538 6301 7820 882
Size: 617 723 1793 1523 763 1519 2219
Fragments arranged by size:
2219 1793 1523 1519 763 723 617
BsaAI yAC'GTr
Cuts at: 493 1204 2769 5449 6948 493
Size: 711 1565 2680 1499 2702
Fragments arranged by size:
2702 2680 1565 1499 711
BsaBI GATnn'nnATC
Cuts at: 3216 9151 3216
Size: 5935 3222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5822 6245 6964 7346
Size: 53 83 186 936 4289 423 719 382
Cuts at: 7346 7981 275
Size: 635 1451
Fragments arranged by size:
4289 1451 936 719 635 423 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2277 5628 5724 5886 7590 7988 8262 8409 8820
Size: 3351 96 162 1704 398 274 147 411
Cuts at: 8820 2277
Size: 2614
Fragments arranged by size:
3351 2614 1704 411 398 274 162 147
96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 2375 2405 3104
Size: 30 165 30 771 30 171 30 699
Cuts at: 3104 3134 3492 3522 4631 4661 4673 4703 5615
Size: 30 358 30 1109 30 12 30 912
Cuts at: 5615 5645 5663 5693 1178
Size: 30 18 30 4642
Fragments arranged by size:
4642 1109 912 771 699 358 171 165
30 30 30 30 30 30 30 30
30 30 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2383 2618 3254 3362 4652 4883 5080 5526
Size: 1197 235 636 108 1290 231 197 446
Cuts at: 5526 1186
Size: 4817
Fragments arranged by size:
4817 1290 1197 636 446 235 231 197
108
BseYI C'CCAG_C
Cuts at: 2102 2592 8360 8856 2102
Size: 490 5768 496 2403
Fragments arranged by size:
5768 2403 496 490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3884 5571 5654 1163
Size: 573 2148 1687 83 4666
Fragments arranged by size:
4666 2148 1687 573 83
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3563 4635 5146 5342 6607 7368
Size: 275 186 1562 1072 511 196 1265 761
Cuts at: 7368 8396 1540
Size: 1028 2301
Fragments arranged by size:
2301 1562 1265 1072 1028 761 511 275
196 186
BsiHKAI G_wGCw'C
Cuts at: 2083 2937 4815 5448 6047 7218 7303 8374 2083
Size: 854 1878 633 599 1171 85 1071 2866
Fragments arranged by size:
2866 1878 1171 1071 854 633 599 85
BsmI GAATG_Cn'
Cuts at: 4831 6668 6761 4831
Size: 1837 93 7227
Fragments arranged by size:
7227 1837 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3715 3715
Size: 9157
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2099 2135 2393 2781 2835 2937
Size: 399 293 16 36 258 388 54 102
Cuts at: 2937 2955 3539 3588 4065 4815 5013 5448 5520
Size: 18 584 49 477 750 198 435 72
Cuts at: 5520 5858 6008 6047 6075 6274 6476 7218 7303
Size: 338 150 39 28 199 202 742 85
Cuts at: 7303 8374 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 750 742 584 477 435 399
388 338 293 258 202 199 198 150
102 85 72 54 49 39 36 28
18 16
BspEI T'CCGG_A
Cuts at: 8820 8820
Size: 9157
BspHI T'CATG_A
Cuts at: 3510 3963 4428 7046 8776 3510
Size: 453 465 2618 1730 3891
Fragments arranged by size:
3891 2618 1730 465 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 4929 5593 5940 6512 844
Size: 2452 1633 664 347 572 3489
Fragments arranged by size:
3489 2452 1633 664 572 347
BsrBI CCG'CTC
Cuts at: 1394 1823 2018 5175 5349 5476 7044 1394
Size: 429 195 3157 174 127 1568 3507
Fragments arranged by size:
3507 3157 1568 429 195 174 127
BsrDI GCAATG_nn'
Cuts at: 65 1248 2112 3634 4154 4293 4546 5236 6418
Size: 1183 864 1522 520 139 253 690 1182
Cuts at: 6418 7652 7826 65
Size: 1234 174 1396
Fragments arranged by size:
1522 1396 1234 1183 1182 864 690 520
253 174 139
BsrFI r'CCGG_y
Cuts at: 1700 1810 4971 5628 5800 5901 6289 7801 7988
Size: 110 3161 657 172 101 388 1512 187
Cuts at: 7988 1700
Size: 2869
Fragments arranged by size:
3161 2869 1512 657 388 187 172 110
101
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9157
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9157
BssSI C'ACGA_G
Cuts at: 2126 2165 2978 7217 8229 2126
Size: 39 813 4239 1012 3054
Fragments arranged by size:
4239 3054 1012 813 39
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5646 794
Size: 4852 4305
BstBI TT'CG_AA
Cuts at: 7961 7961
Size: 9157
BstEII G'GTnAC_C
Cuts at: 1184 3445 4249 1184
Size: 2261 804 6092
Fragments arranged by size:
6092 2261 804
BstXI CCAn_nnnn'nTGG
Cuts at: 2788 3895 3995 4726 4985 2788
Size: 1107 100 731 259 6960
Fragments arranged by size:
6960 1107 731 259 100
BstYI r'GATC_y
Cuts at: 1972 2283 2334 2559 2649 6487 6854 7239 7256
Size: 311 51 225 90 3838 367 385 17
Cuts at: 7256 8708 8794 8806 9152 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3838 1977 1452 385 367 346 311 225
90 86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2193 2383 3717 4488 4621 6199 6231
Size: 552 1128 190 1334 771 133 1578 32
Cuts at: 6231 513
Size: 3439
Fragments arranged by size:
3439 1578 1334 1128 771 552 190 133
32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2311 487
Size: 683 56 1085 7333
Fragments arranged by size:
7333 1085 683 56
BtsI GCAGTG_nn'
Cuts at: 2907 3537 3942 4212 4267 4665 4996 6075 6673
Size: 630 405 270 55 398 331 1079 598
Cuts at: 6673 7467 7487 2907
Size: 794 20 4577
Fragments arranged by size:
4577 1079 794 630 598 405 398 331
270 55 20
ClaI AT'CG_AT
Cuts at: 1554 5004 1554
Size: 3450 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9122
DraI TTT'AAA
Cuts at: 6461 6461
Size: 9157
DraIII CAC_nnn'GTG
Cuts at: 5569 5569
Size: 9157
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8164 809
Size: 507 6848 1802
Fragments arranged by size:
6848 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2061 2148
Size: 54 1425 50 275 18 222 9 87
Cuts at: 2148 2733 3933 4485 4716 5155 5289 6604 7493
Size: 585 1200 552 231 439 134 1315 889
Cuts at: 7493 9122 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1315 1200 889 585 552 439
275 231 222 134 87 54 50 43
18 9
EagI C'GGCC_G
Cuts at: 1537 1812 6604 1537
Size: 275 4792 4090
Fragments arranged by size:
4792 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2978 3195 3509 4883 7087 1353
Size: 734 891 217 314 1374 2204 3423
Fragments arranged by size:
3423 2204 1374 891 734 314 217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7744 8118 8264 387
Size: 1064 138 6155 374 146 1280
Fragments arranged by size:
6155 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2081 2081
Size: 9157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3040 3219 3257 3727 4864 4949 5291
Size: 393 1408 179 38 470 1137 85 342
Cuts at: 5291 5356 6205 6391 7220 7817 8604 8872 1239
Size: 65 849 186 829 597 787 268 1524
Fragments arranged by size:
1524 1408 1137 849 829 787 597 470
393 342 268 186 179 85 65 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3239 3292 5936 3239
Size: 53 2644 6460
Fragments arranged by size:
6460 2644 53
EcoO109I rG'GnC_Cy
Cuts at: 2608 3519 5516 5517 6271 2608
Size: 911 1997 1 754 5494
Fragments arranged by size:
5494 1997 911 754 1
EcoRI G'AATT_C
Cuts at: 6466 6844 6466
Size: 378 8779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5386 5386
Size: 9157
FspI TGC'GCA
Cuts at: 4712 7663 4712
Size: 2951 6206
HaeII r_GCGC'y
Cuts at: 1678 3672 4327 4645 4767 5368 8304 1678
Size: 1994 655 318 122 601 2936 2531
Fragments arranged by size:
2936 2531 1994 655 601 318 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2408 2440 2641
Size: 32 135 32 683 32 121 32 201
Cuts at: 2641 2673 2912 2944 3492 3524 3545 3577 4631
Size: 32 239 32 548 32 21 32 1054
Cuts at: 4631 4663 4829 4861 5615 5647 7798 7830 7872
Size: 32 166 32 754 32 2151 32 42
Cuts at: 7872 7904 1373
Size: 32 2626
Fragments arranged by size:
2626 2151 1054 754 683 548 239 201
166 135 121 42 32 32 32 32
32 32 32 32 32 32 32 32
32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 3574 6501 6747 669
Size: 988 281 1636 2927 246 3079
Fragments arranged by size:
3079 2927 1636 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2463 748
Size: 1715 7442
HpaI GTT'AAC
Cuts at: 6747 6747
Size: 9157
KpnI G_GTAC'C
Cuts at: 2201 5585 6482 2201
Size: 3384 897 4876
Fragments arranged by size:
4876 3384 897
MfeI C'AATT_G
Cuts at: 2451 6756 2451
Size: 4305 4852
MluI A'CGCG_T
Cuts at: 9033 9033
Size: 9157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 4567 4716 4804 6384 8271 8455 1305
Size: 858 2404 149 88 1580 1887 184 2007
Fragments arranged by size:
2404 2007 1887 1580 858 184 149 88
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 2735 3935 5157 10
Size: 54 1425 343 318 585 1200 1222 4010
Fragments arranged by size:
4010 1425 1222 1200 585 343 318 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 3530 5687 5924 6423 7115 7474
Size: 648 960 1404 2157 237 499 692 359
Cuts at: 7474 7633 518
Size: 159 2042
Fragments arranged by size:
2157 2042 1404 960 692 648 499 359
237 159
MspA1I CmG'CkG
Cuts at: 1606 4441 4614 4927 5508 5577 5655 6035 6573
Size: 2835 173 313 581 69 78 380 538
Cuts at: 6573 7250 8398 8643 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2835 2120 1148 677 581 538 380 313
245 173 78 69
NaeI GCC'GGC
Cuts at: 5802 5903 5802
Size: 101 9056
NcoI C'CATG_G
Cuts at: 513 1065 4488 4621 6199 513
Size: 552 3423 133 1578 3471
Fragments arranged by size:
3471 3423 1578 552 133
NdeI CA'TA_TG
Cuts at: 387 2848 387
Size: 2461 6696
NgoMIV G'CCGG_C
Cuts at: 5800 5901 5800
Size: 101 9056
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9157
NotI GC'GGCC_GC
Cuts at: 6604 6604
Size: 9157
NsiI A_TGCA'T
Cuts at: 4113 4156 4113
Size: 43 9114
NspI r_CATG'y
Cuts at: 1132 1229 2626 2926 4115 4477 6022 6121 6422
Size: 97 1397 300 1189 362 1545 99 301
Cuts at: 6422 6515 8060 1132
Size: 93 1545 2229
Fragments arranged by size:
2229 1545 1545 1397 1189 362 301 300
99 97 93
PasI CC'CwG_GG
Cuts at: 1348 6390 1348
Size: 5042 4115
PciI A'CATG_T
Cuts at: 2622 4473 6018 8056 2622
Size: 1851 1545 2038 3723
Fragments arranged by size:
3723 2038 1851 1545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2040 2293 4627 1108
Size: 932 253 2334 5638
Fragments arranged by size:
5638 2334 932 253
PmeI GTTT'AAAC
Cuts at: 6461 6461
Size: 9157
PmlI CAC'GTG
Cuts at: 2769 2769
Size: 9157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4895 4927 7226 7258 8739 8771 4895
Size: 32 2299 32 1481 32 5281
Fragments arranged by size:
5281 2299 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2608 2608
Size: 9157
PshAI GACnn'nnGTC
Cuts at: 1613 4786 1613
Size: 3173 5984
PsiI TTA'TAA
Cuts at: 3759 6727 3759
Size: 2968 6189
PspOMI G'GGCC_C
Cuts at: 5516 6270 5516
Size: 754 8403
PstI C_TGCA'G
Cuts at: 830 3159 4350 4926 5787 5937 6509 830
Size: 2329 1191 576 861 150 572 3478
Fragments arranged by size:
3478 2329 1191 861 576 572 150
PvuI CG_AT'CG
Cuts at: 2001 3563 4635 2001
Size: 1562 1072 6523
Fragments arranged by size:
6523 1562 1072
PvuII CAG'CTG
Cuts at: 4441 4614 4927 5655 6035 4441
Size: 173 313 728 380 7563
Fragments arranged by size:
7563 728 380 313 173
RsrII CG'GwC_CG
Cuts at: 5631 5631
Size: 9157
SacI G_AGCT'C
Cuts at: 2083 2083
Size: 9157
SalI G'TCGA_C
Cuts at: 1936 3572 6499 1936
Size: 1636 2927 4594
Fragments arranged by size:
4594 2927 1636
SapI GCTCTTCn'nnn_
Cuts at: 2087 3195 2087
Size: 1108 8049
SbfI CC_TGCA'GG
Cuts at: 5787 6509 5787
Size: 722 8435
ScaI AGT'ACT
Cuts at: 3167 3740 7405 9117 3167
Size: 573 3665 1712 3207
Fragments arranged by size:
3665 3207 1712 573
SexAI A'CCwGG_T
Cuts at: 3441 3987 3441
Size: 546 8611
SfcI C'TryA_G
Cuts at: 826 1045 3155 4346 4609 4922 5149 5783 5933
Size: 219 2110 1191 263 313 227 634 150
Cuts at: 5933 6505 7640 8321 8512 826
Size: 572 1135 681 191 1471
Fragments arranged by size:
2110 1471 1191 1135 681 634 572 313
263 227 219 191 150
SfiI GGCCn_nnn'nGGCC
Cuts at: 2060 2060
Size: 9157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9157
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9157
SmaI CCC'GGG
Cuts at: 1872 2204 6484 1872
Size: 332 4280 4545
Fragments arranged by size:
4545 4280 332
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2663 3398 7261 8162 8424 820
Size: 197 926 720 735 3863 901 262 1553
Fragments arranged by size:
3863 1553 926 901 735 720 262 197
SnaBI TAC'GTA
Cuts at: 493 6948 493
Size: 6455 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9157
SphI G_CATG'C
Cuts at: 1132 4115 6515 1132
Size: 2983 2400 3774
Fragments arranged by size:
3774 2983 2400
SspI AAT'ATT
Cuts at: 5 52 4087 7081 5
Size: 47 4035 2994 2081
Fragments arranged by size:
4035 2994 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9157
StyI C'CwwG_G
Cuts at: 513 1065 2703 4488 4621 5591 5868 6199 6596
Size: 552 1638 1785 133 970 277 331 397
Cuts at: 6596 513
Size: 3074
Fragments arranged by size:
3074 1785 1638 970 552 397 331 277
133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 2990 3670 4305 4547 5057 5474
Size: 1255 666 744 680 635 242 510 417
Cuts at: 5474 7184 7201 7354 7539 7984 325
Size: 1710 17 153 185 445 1498
Fragments arranged by size:
1710 1498 1255 744 680 666 635 510
445 417 242 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3165 3738 7403
Size: 274 80 33 51 1443 1188 573 3665
Cuts at: 7403 9115 96
Size: 1712 138
Fragments arranged by size:
3665 1712 1443 1188 573 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 2375 2407 3104 3136 4029 4061 5663
Size: 32 169 32 697 32 893 32 1602
Cuts at: 5663 5695 6173 6205 9117 9149 2174
Size: 32 478 32 2912 32 2182
Fragments arranged by size:
2912 2182 1602 893 697 478 169 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5501 6146 1316
Size: 4185 645 4327
Fragments arranged by size:
4327 4185 645
XbaI T'CTAG_A
Cuts at: 6493 6493
Size: 9157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2041 4310 6289 2041
Size: 2269 1979 4909
Fragments arranged by size:
4909 2269 1979
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9157
XmaI C'CCGG_G
Cuts at: 1870 2202 6482 1870
Size: 332 4280 4545
Fragments arranged by size:
4545 4280 332
XmnI GAAnn'nnTTC
Cuts at: 4877 7286 4877
Size: 2409 6748
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5823 6965 276
Size: 53 83 186 936 4289 1142 2468
Fragments arranged by size:
4289 2468 1142 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI
PacI PfoI PspXI PsrI SacII SanDI SfoI SrfI
SwaI