Restriction map for
pFN21AE2670
(Circular) (Six-base) MAPSORT of: pFN21AE2670.seq Check: 4659 from: 1 to: 6838
pFN21AE2670 6838 bp
With 182 enzymes: SgfI *
February 2, 2017 15:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3184 3239 4181 1937
Size: 1247 55 942 4594
Fragments arranged by size:
4594 1247 942 55
Acc65I G'GTAC_C
Cuts at: 2804 4159 2804
Size: 1355 5483
AclI AA'CG_TT
Cuts at: 1563 2473 4965 5338 1563
Size: 910 2492 373 3063
Fragments arranged by size:
3063 2492 910 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2625 4052 4901 6285 6553 1632
Size: 993 1427 849 1384 268 1917
Fragments arranged by size:
1917 1427 1384 993 849 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 2034 5737 6714 1203
Size: 831 3703 977 1327
Fragments arranged by size:
3703 1327 977 831
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6838
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6838
AlwNI CAG_nnn'CTG
Cuts at: 4049 4581 6153 4049
Size: 532 1572 4734
Fragments arranged by size:
4734 1572 532
ApaLI G'TGCA_C
Cuts at: 2026 4895 6051 2026
Size: 2869 1156 2813
Fragments arranged by size:
2869 2813 1156
ApoI r'AATT_y
Cuts at: 1493 2528 4147 4373 4525 6634 1493
Size: 1035 1619 226 152 2109 1697
Fragments arranged by size:
2109 1697 1619 1035 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5392 160
Size: 872 4360 1606
Fragments arranged by size:
4360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 3468 4163 1870
Size: 73 1525 695 4545
Fragments arranged by size:
4545 1525 695 73
BamHI G'GATC_C
Cuts at: 3061 3890 3961 4168 4535 3061
Size: 829 71 207 367 5364
Fragments arranged by size:
5364 829 367 207 71
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2386 2596 2804 4159 5614
Size: 325 217 627 599 210 208 1355 1455
Cuts at: 5614 618
Size: 1842
Fragments arranged by size:
1842 1455 1355 627 599 325 217 210
208
BanII G_rGCy'C
Cuts at: 1391 2964 3031 3073 3453 3753 4020 4157 1391
Size: 1573 67 42 380 300 267 137 4072
Fragments arranged by size:
4072 1573 380 300 267 137 67 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4001 928
Size: 971 2102 3765
Fragments arranged by size:
3765 2102 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5029 5063 1903
Size: 34 3092 34 3678
Fragments arranged by size:
3678 3092 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4730 5940 3428
Size: 1302 1210 4326
Fragments arranged by size:
4326 1302 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6838
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5449 136
Size: 107 122 71 631 605 3777 1525
Fragments arranged by size:
3777 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4025 6833 4025
Size: 2808 4030
BlpI GC'TnA_GC
Cuts at: 4255 4255
Size: 6838
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2273 2601 4899 6055 1790
Size: 240 243 328 2298 1156 2573
Fragments arranged by size:
2573 2298 1156 328 243 240
BmgBI CAC'GTC
Cuts at: 1318 3520 1318
Size: 2202 4636
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 3738 4569
Size: 416 53 83 399 24 2030 291 831
Cuts at: 4569 5527 442
Size: 958 1753
Fragments arranged by size:
2030 1753 958 831 416 399 291 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 2402 2683 2888 3895 3993 4006 5498
Size: 796 367 281 205 1007 98 13 1492
Cuts at: 5498 1239
Size: 2579
Fragments arranged by size:
2579 1492 1007 796 367 281 205 98
13
Bpu10I CC'TnA_GC
Cuts at: 2958 3966 2958
Size: 1008 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3105 3497 3668 4963 5828 6126 3105
Size: 392 171 1295 865 298 3817
Fragments arranged by size:
3817 1295 865 392 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3676 5501 882
Size: 617 2177 1825 2219
Fragments arranged by size:
2219 2177 1825 617
BsaAI yAC'GTr
Cuts at: 493 1204 4629 493
Size: 711 3425 2702
Fragments arranged by size:
3425 2702 711
BsaBI GATnn'nnATC
Cuts at: 6832 6832
Size: 6838
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4645 5027 5662 275
Size: 53 83 186 936 3112 382 635 1451
Fragments arranged by size:
3112 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2115 5271 5669 5943 6090 6501 2115
Size: 3156 398 274 147 411 2452
Fragments arranged by size:
3156 2452 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2956 2986 3060 3090 1178
Size: 30 165 30 1553 30 74 30 4926
Fragments arranged by size:
4926 1553 165 74 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2389 2526 1186
Size: 852 351 137 5498
Fragments arranged by size:
5498 852 351 137
BseYI C'CCAG_C
Cuts at: 2286 2713 3101 3135 3359 6041 6537 2286
Size: 427 388 34 224 2682 496 2587
Fragments arranged by size:
2682 2587 496 427 388 224 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2798 3076 1163
Size: 573 1062 278 4925
Fragments arranged by size:
4925 1062 573 278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 2964 3542 4899 4984 6055 2030
Size: 934 578 1357 85 1071 2813
Fragments arranged by size:
2813 1357 1071 934 578 85
BsmI GAATG_Cn'
Cuts at: 2111 2851 4349 4442 2111
Size: 740 1498 93 4507
Fragments arranged by size:
4507 1498 740 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2273 2601 2964 3031 3073 3453
Size: 399 240 243 328 363 67 42 380
Cuts at: 3453 3542 3753 4020 4157 4899 4984 6055 1391
Size: 89 211 267 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 363 328 267
243 240 211 137 89 85 67 42
BspEI T'CCGG_A
Cuts at: 2115 6501 2115
Size: 4386 2452
BspHI T'CATG_A
Cuts at: 4727 6457 4727
Size: 1730 5108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3297 4126 4193 844
Size: 2453 829 67 3489
Fragments arranged by size:
3489 2453 829 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4725 1394
Size: 429 2902 3507
Fragments arranged by size:
3507 2902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5333 5507 65
Size: 1183 4085 174 1396
Fragments arranged by size:
4085 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5482 5669 1700
Size: 110 3672 187 2869
Fragments arranged by size:
3672 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6838
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 4898 5910 4898
Size: 1012 5826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6838
BstBI TT'CG_AA
Cuts at: 5642 5642
Size: 6838
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6838
BstYI r'GATC_y
Cuts at: 1972 3061 3890 3961 4025 4115 4168 4535 4920
Size: 1089 829 71 64 90 53 367 385
Cuts at: 4920 4937 6389 6475 6487 6833 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1089 829 385 367 346 90
86 71 64 53 17 12
BstZ17I GTA'TAC
Cuts at: 3240 3240
Size: 6838
Bsu36I CC'TnA_GG
Cuts at: 2402 3944 2402
Size: 1542 5296
BtgI C'CryG_G
Cuts at: 513 1065 3143 513
Size: 552 2078 4208
Fragments arranged by size:
4208 2078 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 2490 3699 4354 5148 5168 2490
Size: 1209 655 794 20 4160
Fragments arranged by size:
4160 1209 794 655 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 4142 4142
Size: 6838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5845 809
Size: 507 4529 1802
Fragments arranged by size:
4529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2292 2730 4285
Size: 54 1425 50 275 18 462 438 1555
Cuts at: 4285 5174 6803 8
Size: 889 1629 43
Fragments arranged by size:
1629 1555 1425 889 462 438 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2544 3000 4768 1353
Size: 1191 456 1768 3423
Fragments arranged by size:
3423 1768 1191 456
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2962 2962
Size: 6838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2402 2625 2683 2888 3895 3993
Size: 393 403 367 223 58 205 1007 98
Cuts at: 3993 4006 4052 4901 5498 6285 6553 1239
Size: 13 46 849 597 787 268 1524
Fragments arranged by size:
1524 1007 849 787 597 403 393 367
268 223 205 98 58 46 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2793 2793
Size: 6838
EcoO109I rG'GnC_Cy
Cuts at: 3727 3881 3727
Size: 154 6684
EcoRI G'AATT_C
Cuts at: 4147 4525 4147
Size: 378 6460
EcoRV GAT'ATC
Cuts at: 2179 2179
Size: 6838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4082 4082
Size: 6838
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 5985 1678
Size: 4307 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 5479 5511 5553
Size: 32 135 32 554 32 3321 32 42
Cuts at: 5553 5585 1373
Size: 32 2626
Fragments arranged by size:
3321 2626 554 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2557 4182 4428 669
Size: 988 281 619 1625 246 3079
Fragments arranged by size:
3079 1625 988 619 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6838
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KpnI G_GTAC'C
Cuts at: 2808 4163 2808
Size: 1355 5483
MfeI C'AATT_G
Cuts at: 4437 4437
Size: 6838
MluI A'CGCG_T
Cuts at: 2034 6714 2034
Size: 4680 2158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 2326 2637 3812 3852 5952 6136 1305
Size: 744 277 311 1175 40 2100 184 2007
Fragments arranged by size:
2100 2007 1175 744 311 277 184 40
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5016
Fragments arranged by size:
5016 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4796 5155 5314 518
Size: 648 3630 359 159 2042
Fragments arranged by size:
3630 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2603 2885 2994 3689 3737 3897 4254 4931
Size: 997 282 109 695 48 160 357 677
Cuts at: 4931 6079 6324 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 997 695 677 357 282 245
160 109 48
NcoI C'CATG_G
Cuts at: 513 1065 3143 513
Size: 552 2078 4208
Fragments arranged by size:
4208 2078 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 4196 5741 1132
Size: 97 2967 1545 2229
Fragments arranged by size:
2967 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3048 1348
Size: 1700 5138
PciI A'CATG_T
Cuts at: 5737 5737
Size: 6838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3161 1108
Size: 2053 4785
PfoI T'CCnGG_A
Cuts at: 3064 3064
Size: 6838
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2735 2767 4907 4939 6420 6452 2735
Size: 32 2140 32 1481 32 3121
Fragments arranged by size:
3121 2140 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3727 3881 3727
Size: 154 6684
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PstI C_TGCA'G
Cuts at: 830 2999 3760 4190 830
Size: 2169 761 430 3478
Fragments arranged by size:
3478 2169 761 430
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
PvuII CAG'CTG
Cuts at: 2885 2994 3737 3897 2885
Size: 109 743 160 5826
Fragments arranged by size:
5826 743 160 109
SacI G_AGCT'C
Cuts at: 2964 2964
Size: 6838
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 5086 6798 5086
Size: 1712 5126
SfcI C'TryA_G
Cuts at: 826 1045 2995 3732 3756 3981 4186 5321 6002
Size: 219 1950 737 24 225 205 1135 681
Cuts at: 6002 6193 826
Size: 191 1471
Fragments arranged by size:
1950 1471 1135 737 681 225 219 205
191 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 4165 1872
Size: 2293 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3120 3468 3512 3647 4942 5843
Size: 197 926 1177 348 44 135 1295 901
Cuts at: 5843 6105 820
Size: 262 1553
Fragments arranged by size:
1553 1295 1177 926 901 348 262 197
135 44
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 4196 1132
Size: 3064 3774
SspI AAT'ATT
Cuts at: 5 52 4762 5
Size: 47 4710 2081
Fragments arranged by size:
4710 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6838
StyI C'CwwG_G
Cuts at: 513 1065 3143 4277 513
Size: 552 2078 1134 3074
Fragments arranged by size:
3074 2078 1134 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2243 2477 2633 4865 4882 5035 5220
Size: 1255 663 234 156 2232 17 153 185
Cuts at: 5220 5665 325
Size: 445 1498
Fragments arranged by size:
2232 1498 1255 663 445 234 185 156
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5084 6796 96
Size: 274 80 33 51 1443 3107 1712 138
Fragments arranged by size:
3107 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2956 2988 6798 6830 2956
Size: 32 3810 32 2964
Fragments arranged by size:
3810 2964 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6838
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2237 4010 2237
Size: 1773 5065
XhoI C'TCGA_G
Cuts at: 1943 3468 1943
Size: 1525 5313
XmaI C'CCGG_G
Cuts at: 1870 4163 1870
Size: 2293 4545
XmnI GAAnn'nnTTC
Cuts at: 4967 4967
Size: 6838
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI