Restriction map for
pFN21AE2667
(Circular) (Six-base) MAPSORT of: pFN21AE2667.seq Check: 9706 from: 1 to: 8020
pFN21AE2667 8020 bp
With 182 enzymes: SgfI *
January 20, 2017 10:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4513 4831 4513
Size: 318 7702
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5830 278
Size: 53 83 186 936 4294 2468
Fragments arranged by size:
4294 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2079 5363 1937
Size: 142 3284 4594
Fragments arranged by size:
4594 3284 142
Acc65I G'GTAC_C
Cuts at: 5341 5341
Size: 8020
AclI AA'CG_TT
Cuts at: 1563 5319 6147 6520 1563
Size: 3756 828 373 3063
Fragments arranged by size:
3756 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 2900 3729 4094 6083 7467 7735 1632
Size: 674 594 829 365 1989 1384 268 1917
Fragments arranged by size:
1989 1917 1384 829 674 594 365 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7823
AflIII A'CryG_T
Cuts at: 1203 6919 7896 1203
Size: 5716 977 1327
Fragments arranged by size:
5716 1327 977
AgeI A'CCGG_T
Cuts at: 6851 6851
Size: 8020
AhdI GACnn_n'nnGTC
Cuts at: 3817 4164 6749 3817
Size: 347 2585 5088
Fragments arranged by size:
5088 2585 347
AleI CACnn'nnGTG
Cuts at: 1166 2036 1166
Size: 870 7150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8020
AlwNI CAG_nnn'CTG
Cuts at: 3626 4528 5260 5763 7335 3626
Size: 902 732 503 1572 4311
Fragments arranged by size:
4311 1572 902 732 503
ApaI G_GGCC'C
Cuts at: 3172 3412 3533 3724 4588 3172
Size: 240 121 191 864 6604
Fragments arranged by size:
6604 864 240 191 121
ApaLI G'TGCA_C
Cuts at: 6077 7233 6077
Size: 1156 6864
ApoI r'AATT_y
Cuts at: 1493 3293 5329 5555 5707 7816 1493
Size: 1800 2036 226 152 2109 1697
Fragments arranged by size:
2109 2036 1800 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6574 160
Size: 872 5542 1606
Fragments arranged by size:
5542 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8020
AvaI C'yCGr_G
Cuts at: 1870 1943 2378 2682 4170 4781 5345 1870
Size: 73 435 304 1488 611 564 4545
Fragments arranged by size:
4545 1488 611 564 435 304 73
BamHI G'GATC_C
Cuts at: 5350 5717 5350
Size: 367 7653
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2477 2863 3484 4257 5290
Size: 325 217 627 690 386 621 773 1033
Cuts at: 5290 5341 6796 618
Size: 51 1455 1842
Fragments arranged by size:
1842 1455 1033 773 690 627 621 386
325 217 51
BanII G_rGCy'C
Cuts at: 1391 2499 3137 3172 3258 3412 3533 3645 3724
Size: 1108 638 35 86 154 121 112 79
Cuts at: 3724 4296 4304 4588 5339 1391
Size: 572 8 284 751 4072
Fragments arranged by size:
4072 1108 751 638 572 284 154 121
112 86 79 35 8
BbeI G_GCGC'C
Cuts at: 2867 4261 2867
Size: 1394 6626
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2347 3735 928
Size: 971 448 1388 5213
Fragments arranged by size:
5213 1388 971 448
BbvCI CC'TCA_GC
Cuts at: 3162 3979 3162
Size: 817 7203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5009 5043 6211 6245 1903
Size: 34 50 34 2988 34 1168 34 3678
Fragments arranged by size:
3678 2988 1168 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5912 7122 5912
Size: 1210 6810
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8020
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3691 4346 4950
Size: 107 122 71 631 605 2019 655 604
Cuts at: 4950 5000 5024 6631 136
Size: 50 24 1607 1525
Fragments arranged by size:
2019 1607 1525 655 631 605 604 122
107 71 50 24
BglII A'GATC_T
Cuts at: 8015 8015
Size: 8020
BlpI GC'TnA_GC
Cuts at: 2558 3632 5437 2558
Size: 1074 1805 5141
Fragments arranged by size:
5141 1805 1074
Bme1580I G_kGCm'C
Cuts at: 1790 2314 3172 3412 3489 3533 3724 4588 4659
Size: 524 858 240 77 44 191 864 71
Cuts at: 4659 6081 7237 1790
Size: 1422 1156 2573
Fragments arranged by size:
2573 1422 1156 864 858 524 240 191
77 71 44
BmgBI CAC'GTC
Cuts at: 1318 2044 2470 1318
Size: 726 426 6868
Fragments arranged by size:
6868 726 426
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2924 4347 4852
Size: 416 53 83 399 24 1507 1423 505
Cuts at: 4852 5751 6709 442
Size: 899 958 1753
Fragments arranged by size:
1753 1507 1423 958 899 505 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2549 2684 2736 3103 3123 4550 4595 4718
Size: 1310 135 52 367 20 1427 45 123
Cuts at: 4718 4794 5243 6680 1239
Size: 76 449 1437 2579
Fragments arranged by size:
2579 1437 1427 1310 449 367 135 123
76 52 45 20
Bpu10I CC'TnA_GC
Cuts at: 3162 3449 3627 3979 3162
Size: 287 178 352 7203
Fragments arranged by size:
7203 352 287 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3557 3917 4280 5327 6145 7010 7308 3557
Size: 360 363 1047 818 865 298 4269
Fragments arranged by size:
4269 1047 865 818 363 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3764 4261 6683 882
Size: 617 2265 497 2422 2219
Fragments arranged by size:
2422 2265 2219 617 497
BsaAI yAC'GTr
Cuts at: 493 1204 2077 2518 3382 5811 493
Size: 711 873 441 864 2429 2702
Fragments arranged by size:
2702 2429 873 864 711 441
BsaBI GATnn'nnATC
Cuts at: 4241 8014 4241
Size: 3773 4247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2484 2790 2864 2984
Size: 53 83 186 936 951 306 74 120
Cuts at: 2984 4258 4953 5827 6209 6844 275
Size: 1274 695 874 382 635 1451
Fragments arranged by size:
1451 1274 951 936 874 695 635 382
306 186 120 83 74 53
BsaWI w'CCGG_w
Cuts at: 2127 2358 2808 6453 6851 7125 7272 7683 2127
Size: 231 450 3645 398 274 147 411 2464
Fragments arranged by size:
3645 2464 450 411 398 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 3187 3217 3575
Size: 30 165 30 693 30 1061 30 358
Cuts at: 3575 3605 3833 3863 4532 4562 5192 5222 1178
Size: 30 228 30 669 30 630 30 3976
Fragments arranged by size:
3976 1061 693 669 630 358 228 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2648 2738 3539 3596 3653 3782 3823 3853
Size: 1462 90 801 57 57 129 41 30
Cuts at: 3853 3967 4015 4018 4027 4030 4033 4957 5195
Size: 114 48 3 9 3 3 924 238
Cuts at: 5195 1186
Size: 4011
Fragments arranged by size:
4011 1462 924 801 238 129 114 90
57 57 48 41 30 9 3 3
3
BseYI C'CCAG_C
Cuts at: 2445 2643 2859 3405 3503 3681 4151 4396 4581
Size: 198 216 546 98 178 470 245 185
Cuts at: 4581 5064 5286 7223 7719 2445
Size: 483 222 1937 496 2746
Fragments arranged by size:
2746 1937 546 496 483 470 245 222
216 198 185 178 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2042 2762 3404 4541 1163
Size: 573 306 720 642 1137 4642
Fragments arranged by size:
4642 1137 720 642 573 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5470 6231 7259 1540
Size: 275 186 3469 761 1028 2301
Fragments arranged by size:
3469 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2627 6081 6166 7237 2627
Size: 3454 85 1071 3410
Fragments arranged by size:
3454 3410 1071 85
BsmI GAATG_Cn'
Cuts at: 3605 3952 5531 5624 3605
Size: 347 1579 93 6001
Fragments arranged by size:
6001 1579 347 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5158 5158
Size: 8020
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2314 2499 2627 3137 3172 3258 3412
Size: 399 524 185 128 510 35 86 154
Cuts at: 3412 3489 3533 3645 3724 4296 4304 4588 4659
Size: 77 44 112 79 572 8 284 71
Cuts at: 4659 5339 6081 6166 7237 1391
Size: 680 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 680 572 524 510 399
284 185 154 128 112 86 85 79
77 71 44 35 8
BspEI T'CCGG_A
Cuts at: 2808 7683 2808
Size: 4875 3145
BspHI T'CATG_A
Cuts at: 5909 7639 5909
Size: 1730 6290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2907 2976 4513 4831 5375 844
Size: 1316 747 69 1537 318 544 3489
Fragments arranged by size:
3489 1537 1316 747 544 318 69
BsrBI CCG'CTC
Cuts at: 1394 1823 2607 4083 4545 5907 1394
Size: 429 784 1476 462 1362 3507
Fragments arranged by size:
3507 1476 1362 784 462 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3036 3079 3285 6515 6689 65
Size: 1183 1788 43 206 3230 174 1396
Fragments arranged by size:
3230 1788 1396 1183 206 174 43
BsrFI r'CCGG_y
Cuts at: 1700 1810 2924 3690 3700 6664 6851 1700
Size: 110 1114 766 10 2964 187 2869
Fragments arranged by size:
2964 2869 1114 766 187 110 10
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8020
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8020
BssSI C'ACGA_G
Cuts at: 6080 7092 6080
Size: 1012 7008
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8020
BstBI TT'CG_AA
Cuts at: 6824 6824
Size: 8020
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8020
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2506 2662 4286 2038
Size: 468 156 1624 5772
Fragments arranged by size:
5772 1624 468 156
BstYI r'GATC_y
Cuts at: 1972 2238 5256 5350 5717 6102 6119 7571 7657
Size: 266 3018 94 367 385 17 1452 86
Cuts at: 7657 7669 8015 1972
Size: 12 346 1977
Fragments arranged by size:
3018 1977 1452 385 367 346 266 94
86 17 12
BstZ17I GTA'TAC
Cuts at: 2080 2080
Size: 8020
Bsu36I CC'TnA_GG
Cuts at: 3661 4801 3661
Size: 1140 6880
BtgI C'CryG_G
Cuts at: 513 1065 2034 3093 3563 3645 4105 4285 4506
Size: 552 969 1059 470 82 460 180 221
Cuts at: 4506 5211 513
Size: 705 3322
Fragments arranged by size:
3322 1059 969 705 552 470 460 221
180 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7281
Fragments arranged by size:
7281 683 56
BtsI GCAGTG_nn'
Cuts at: 3094 4977 5536 6330 6350 3094
Size: 1883 559 794 20 4764
Fragments arranged by size:
4764 1883 794 559 20
ClaI AT'CG_AT
Cuts at: 1554 2295 3426 1554
Size: 741 1131 6148
Fragments arranged by size:
6148 1131 741
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4045 4080 529
Size: 35 3481 35 4469
Fragments arranged by size:
4469 3481 35 35
DraI TTT'AAA
Cuts at: 5324 5324
Size: 8020
DraIII CAC_nnn'GTG
Cuts at: 2345 2345
Size: 8020
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3113 7027 809
Size: 507 1797 3914 1802
Fragments arranged by size:
3914 1802 1797 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2191 2922 3147
Size: 54 1425 50 275 18 361 731 225
Cuts at: 3147 3797 4932 5016 5467 6356 7985 8
Size: 650 1135 84 451 889 1629 43
Fragments arranged by size:
1629 1425 1135 889 731 650 451 361
275 225 84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5467 1537
Size: 275 3655 4090
Fragments arranged by size:
4090 3655 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2273 2715 3712 3759 4036 4051 5950
Size: 896 24 442 997 47 277 15 1899
Cuts at: 5950 1353
Size: 3423
Fragments arranged by size:
3423 1899 997 896 442 277 47 24
15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6607 6981 7127 387
Size: 1064 138 5018 374 146 1280
Fragments arranged by size:
5018 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2549 2684 2736 2900 3103 3123
Size: 393 674 243 135 52 164 203 20
Cuts at: 3123 3729 4094 4550 4595 4718 4794 5243 6083
Size: 606 365 456 45 123 76 449 840
Cuts at: 6083 6680 7467 7735 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 840 787 674 606 597 456 449
393 365 268 243 203 164 135 123
76 52 45 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2882 3764 2882
Size: 882 7138
EcoO109I rG'GnC_Cy
Cuts at: 2200 2647 3031 3409 3529 3530 4585 4854 2200
Size: 447 384 378 120 1 1055 269 5366
Fragments arranged by size:
5366 1055 447 384 378 269 120 1
EcoRI G'AATT_C
Cuts at: 3293 5329 5707 3293
Size: 2036 378 5606
Fragments arranged by size:
5606 2036 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 8020
FseI GG_CCGG'CC
Cuts at: 2928 2928
Size: 8020
FspI TGC'GCA
Cuts at: 6526 6526
Size: 8020
HaeII r_GCGC'y
Cuts at: 1678 2867 4261 4470 7167 1678
Size: 1189 1394 209 2697 2531
Fragments arranged by size:
2697 2531 1394 1189 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 3145 3177 3187
Size: 32 135 32 524 32 1017 32 10
Cuts at: 3187 3219 3347 3379 3644 3676 3803 3833 3835
Size: 32 128 32 265 32 127 30 2
Cuts at: 3835 3865 4150 4182 5192 5224 6661 6693 6735
Size: 30 285 32 1010 32 1437 32 42
Cuts at: 6735 6767 1373
Size: 32 2626
Fragments arranged by size:
2626 1437 1017 1010 524 285 265 135
128 127 42 32 32 32 32 32
32 32 32 32 32 32 30 30
10 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2389 4738 5364 5610 669
Size: 988 281 451 2349 626 246 3079
Fragments arranged by size:
3079 2349 988 626 451 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8020
HpaI GTT'AAC
Cuts at: 5610 5610
Size: 8020
KasI G'GCGC_C
Cuts at: 2863 4257 2863
Size: 1394 6626
KpnI G_GTAC'C
Cuts at: 5345 5345
Size: 8020
MfeI C'AATT_G
Cuts at: 5619 5619
Size: 8020
MluI A'CGCG_T
Cuts at: 7896 7896
Size: 8020
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 2718 3259 4404 4981 5322 7134 7318
Size: 1248 165 541 1145 577 341 1812 184
Cuts at: 7318 1305
Size: 2007
Fragments arranged by size:
2007 1812 1248 1145 577 541 341 184
165
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2193 3149 10
Size: 54 1425 343 361 956 4881
Fragments arranged by size:
4881 1425 956 361 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 5081 5978 6337 6496 518
Size: 648 870 3045 897 359 159 2042
Fragments arranged by size:
3045 2042 897 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2445 3143 3166 3565 3718 4107 4264 4508
Size: 839 698 23 399 153 389 157 244
Cuts at: 4508 4814 4991 5436 6113 7261 7506 1606
Size: 306 177 445 677 1148 245 2120
Fragments arranged by size:
2120 1148 839 698 677 445 399 389
306 245 244 177 157 153 23
NaeI GCC'GGC
Cuts at: 2926 3702 2926
Size: 776 7244
NarI GG'CG_CC
Cuts at: 2864 4258 2864
Size: 1394 6626
NcoI C'CATG_G
Cuts at: 513 1065 5211 513
Size: 552 4146 3322
Fragments arranged by size:
4146 3322 552
NdeI CA'TA_TG
Cuts at: 387 3802 387
Size: 3415 4605
NgoMIV G'CCGG_C
Cuts at: 2924 3700 2924
Size: 776 7244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8020
NotI GC'GGCC_GC
Cuts at: 5467 5467
Size: 8020
NspI r_CATG'y
Cuts at: 1132 1229 4464 5378 6923 1132
Size: 97 3235 914 1545 2229
Fragments arranged by size:
3235 2229 1545 914 97
PasI CC'CwG_GG
Cuts at: 1348 3130 1348
Size: 1782 6238
PciI A'CATG_T
Cuts at: 6919 6919
Size: 8020
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4600 5260 1108
Size: 3492 660 3868
Fragments arranged by size:
3868 3492 660
PfoI T'CCnGG_A
Cuts at: 2262 2505 4482 5196 5259 2262
Size: 243 1977 714 63 5023
Fragments arranged by size:
5023 1977 714 243 63
PmeI GTTT'AAAC
Cuts at: 5324 5324
Size: 8020
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 6089 6121 7602 7634 3962
Size: 32 2095 32 1481 32 4348
Fragments arranged by size:
4348 2095 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2647 2200
Size: 447 7573
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8020
PsiI TTA'TAA
Cuts at: 5590 5590
Size: 8020
PspOMI G'GGCC_C
Cuts at: 3168 3408 3529 3720 4584 3168
Size: 240 121 191 864 6604
Fragments arranged by size:
6604 864 240 191 121
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2126 2158 2126
Size: 32 7988
PstI C_TGCA'G
Cuts at: 830 2157 3717 5372 830
Size: 1327 1560 1655 3478
Fragments arranged by size:
3478 1655 1560 1327
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8020
PvuII CAG'CTG
Cuts at: 2445 3143 4264 2445
Size: 698 1121 6201
Fragments arranged by size:
6201 1121 698
RsrII CG'GwC_CG
Cuts at: 4186 4186
Size: 8020
SacII CC_GC'GG
Cuts at: 3566 4108 4509 3566
Size: 542 401 7077
Fragments arranged by size:
7077 542 401
SalI G'TCGA_C
Cuts at: 1936 5362 1936
Size: 3426 4594
SanDI GG'GwC_CC
Cuts at: 2647 2647
Size: 8020
SbfI CC_TGCA'GG
Cuts at: 5372 5372
Size: 8020
ScaI AGT'ACT
Cuts at: 3278 6268 7980 3278
Size: 2990 1712 3318
Fragments arranged by size:
3318 2990 1712
SexAI A'CCwGG_T
Cuts at: 2096 2096
Size: 8020
SfcI C'TryA_G
Cuts at: 826 1045 2153 3713 5368 6503 7184 7375 826
Size: 219 1108 1560 1655 1135 681 191 1471
Fragments arranged by size:
1655 1560 1471 1135 1108 681 219 191
SfoI GGC'GCC
Cuts at: 2865 4259 2865
Size: 1394 6626
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8020
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8020
SmaI CCC'GGG
Cuts at: 1872 4172 4783 5347 1872
Size: 2300 611 564 4545
Fragments arranged by size:
4545 2300 611 564
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3572 3932 4295 5306 6124 7025
Size: 197 926 1629 360 363 1011 818 901
Cuts at: 7025 7287 820
Size: 262 1553
Fragments arranged by size:
1629 1553 1011 926 901 818 363 360
262 197
SnaBI TAC'GTA
Cuts at: 493 2077 5811 493
Size: 1584 3734 2702
Fragments arranged by size:
3734 2702 1584
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8020
SphI G_CATG'C
Cuts at: 1132 5378 1132
Size: 4246 3774
SspI AAT'ATT
Cuts at: 5 52 5944 5
Size: 47 5892 2081
Fragments arranged by size:
5892 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8020
StyI C'CwwG_G
Cuts at: 513 1065 2889 3480 3513 4116 4662 4884 5004
Size: 552 1824 591 33 603 546 222 120
Cuts at: 5004 5211 5459 513
Size: 207 248 3074
Fragments arranged by size:
3074 1824 603 591 552 546 248 222
207 120 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 4174 4897 5278 6047 6064 6217
Size: 1255 2145 449 723 381 769 17 153
Cuts at: 6217 6402 6847 325
Size: 185 445 1498
Fragments arranged by size:
2145 1498 1255 769 723 449 445 381
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3276 6266 7978
Size: 274 80 33 51 1443 1299 2990 1712
Cuts at: 7978 96
Size: 138
Fragments arranged by size:
2990 1712 1443 1299 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2813 2845 4532 4564 7980 8012 2813
Size: 32 1687 32 3416 32 2821
Fragments arranged by size:
3416 2821 1687 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2098 2351 1316
Size: 782 253 6985
Fragments arranged by size:
6985 782 253
XbaI T'CTAG_A
Cuts at: 5356 5356
Size: 8020
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5011 5218 5261 5011
Size: 207 43 7770
Fragments arranged by size:
7770 207 43
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8020
XmaI C'CCGG_G
Cuts at: 1870 4170 4781 5345 1870
Size: 2300 611 564 4545
Fragments arranged by size:
4545 2300 611 564
XmnI GAAnn'nnTTC
Cuts at: 5276 6149 5276
Size: 873 7147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5828 276
Size: 53 83 186 936 4294 2468
Fragments arranged by size:
4294 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
EcoICRI EcoRV FspAI NruI NsiI PacI PmlI PspXI
SacI SapI SfiI SrfI SwaI