Restriction map for
pFN21AE2652
(Circular) (Six-base) MAPSORT of: pFN21AE2652.seq Check: 778 from: 1 to: 6238
pFN21AE2652 6238 bp
With 182 enzymes: SgfI *
November 2, 2016 16:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3581 1937
Size: 1644 4594
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3519 4301 5685 5953 1632
Size: 1887 782 1384 268 1917
Fragments arranged by size:
1917 1887 1384 782 268
AfeI AGC'GCT
Cuts at: 2925 2925
Size: 6238
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 2652 5137 6114 1203
Size: 1449 2485 977 1327
Fragments arranged by size:
2485 1449 1327 977
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AlwNI CAG_nnn'CTG
Cuts at: 2391 2709 3981 5553 2391
Size: 318 1272 1572 3076
Fragments arranged by size:
3076 1572 1272 318
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 2282 2813 3547 3773 3925 6034 1493
Size: 789 531 734 226 152 2109 1697
Fragments arranged by size:
2109 1697 789 734 531 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2346 3165 3423 4792 160
Size: 872 1314 819 258 1369 1606
Fragments arranged by size:
1606 1369 1314 872 819 258
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 3563 1870
Size: 73 1620 4545
Fragments arranged by size:
4545 1620 73
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3213 3559 5014 618
Size: 325 217 627 1426 346 1455 1842
Fragments arranged by size:
1842 1455 1426 627 346 325 217
BanII G_rGCy'C
Cuts at: 1391 2050 3557 1391
Size: 659 1507 4072
Fragments arranged by size:
4072 1507 659
BbeI G_GCGC'C
Cuts at: 3217 3217
Size: 6238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2534 3371 928
Size: 971 635 837 3795
Fragments arranged by size:
3795 971 837 635
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4849 136
Size: 107 122 71 631 605 3177 1525
Fragments arranged by size:
3177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2264 6233 2264
Size: 3969 2269
BlpI GC'TnA_GC
Cuts at: 3655 3655
Size: 6238
Bme1580I G_kGCm'C
Cuts at: 1790 4299 5455 1790
Size: 2509 1156 2573
Fragments arranged by size:
2573 2509 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2567 2896 3202
Size: 416 53 83 399 24 1150 329 306
Cuts at: 3202 3969 4927 442
Size: 767 958 1753
Fragments arranged by size:
1753 1150 958 767 416 399 329 306
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2682 2991 3432 4898 1239
Size: 1443 309 441 1466 2579
Fragments arranged by size:
2579 1466 1443 441 309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4363 5228 5526 4363
Size: 865 298 5075
Fragments arranged by size:
5075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2179 4901 882
Size: 617 680 2722 2219
Fragments arranged by size:
2722 2219 680 617
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3214 4045 4427 5062
Size: 53 83 186 936 1681 831 382 635
Cuts at: 5062 275
Size: 1451
Fragments arranged by size:
1681 1451 936 831 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4671 5069 5343 5490 5901 4671
Size: 398 274 147 411 5008
Fragments arranged by size:
5008 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6013
Fragments arranged by size:
6013 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2988 1186
Size: 1802 4436
BseYI C'CCAG_C
Cuts at: 2043 5441 5937 2043
Size: 3398 496 2344
Fragments arranged by size:
3398 2344 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2394 3418 1163
Size: 573 658 1024 3983
Fragments arranged by size:
3983 1024 658 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3688 4449 5477 1540
Size: 275 186 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4299 4384 5455 4299
Size: 85 1071 5082
Fragments arranged by size:
5082 1071 85
BsmI GAATG_Cn'
Cuts at: 3749 3842 3749
Size: 93 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 3557 4299 4384 5455 1391
Size: 399 260 1507 742 85 1071 2174
Fragments arranged by size:
2174 1507 1071 742 399 260 85
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 4127 5857 4127
Size: 1730 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3593 844
Size: 2749 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3193 3250 4733 4907 65
Size: 1183 1945 57 1483 174 1396
Fragments arranged by size:
1945 1483 1396 1183 174 57
BsrFI r'CCGG_y
Cuts at: 1700 1810 4882 5069 1700
Size: 110 3072 187 2869
Fragments arranged by size:
3072 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3009 96
Size: 2913 3325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 3057 4298 5310 3057
Size: 1241 1012 3985
Fragments arranged by size:
3985 1241 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3521 794
Size: 2727 3511
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6238
BstYI r'GATC_y
Cuts at: 1972 2264 3502 3568 3935 4320 4337 5789 5875
Size: 292 1238 66 367 385 17 1452 86
Cuts at: 5875 5887 6233 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1238 385 367 346 292 86
66 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5686
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2122 487
Size: 683 56 896 4603
Fragments arranged by size:
4603 896 683 56
BtsI GCAGTG_nn'
Cuts at: 2788 3754 4548 4568 2788
Size: 966 794 20 4458
Fragments arranged by size:
4458 966 794 20
ClaI AT'CG_AT
Cuts at: 1554 3208 1554
Size: 1654 4584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2800 3080 3256
Size: 54 1425 50 275 18 970 280 176
Cuts at: 3256 3685 4574 6203 8
Size: 429 889 1629 43
Fragments arranged by size:
1629 1425 970 889 429 280 275 176
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4168 1353
Size: 2815 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4825 5199 5345 387
Size: 1064 138 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2682 2991 3432 3519 4301 4898 5685
Size: 393 1050 309 441 87 782 597 787
Cuts at: 5685 5953 1239
Size: 268 1524
Fragments arranged by size:
1524 1050 787 782 597 441 393 309
268 87
EcoRI G'AATT_C
Cuts at: 2813 3547 3925 2813
Size: 734 378 5126
Fragments arranged by size:
5126 734 378
EcoRV GAT'ATC
Cuts at: 2491 2491
Size: 6238
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 2927 2970 3217 5385 1678
Size: 1249 43 247 2168 2531
Fragments arranged by size:
2531 2168 1249 247 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2855 2887 2913 2945 4879
Size: 32 135 32 1283 32 26 32 1934
Cuts at: 4879 4911 4953 4985 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1934 1283 135 42 32 32 32
32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 3582 3828 669
Size: 988 281 1644 246 3079
Fragments arranged by size:
3079 1644 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6238
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KasI G'GCGC_C
Cuts at: 3213 3213
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 3837 3837
Size: 6238
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3004 5352 5536 1305
Size: 1699 2348 184 2007
Fragments arranged by size:
2348 2007 1699 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3082 3258 10
Size: 54 1425 343 1250 176 2990
Fragments arranged by size:
2990 1425 1250 343 176 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2355 3045 4196 4555 4714 518
Size: 648 1189 690 1151 359 159 2042
Fragments arranged by size:
2042 1189 1151 690 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3654 4331 5479 5724 1606
Size: 2048 677 1148 245 2120
Fragments arranged by size:
2120 2048 1148 677 245
NarI GG'CG_CC
Cuts at: 3214 3214
Size: 6238
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5686
NdeI CA'TA_TG
Cuts at: 387 2352 387
Size: 1965 4273
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 2656 3596 5141 1132
Size: 97 1427 940 1545 2229
Fragments arranged by size:
2229 1545 1427 940 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6238
PciI A'CATG_T
Cuts at: 2652 5137 2652
Size: 2485 3753
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2444 1108
Size: 1336 4902
PfoI T'CCnGG_A
Cuts at: 2715 2715
Size: 6238
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2371 2403 2371
Size: 32 6206
PstI C_TGCA'G
Cuts at: 830 3590 830
Size: 2760 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 4486 6198 4486
Size: 1712 4526
SfcI C'TryA_G
Cuts at: 826 1045 3586 4721 5402 5593 826
Size: 219 2541 1135 681 191 1471
Fragments arranged by size:
2541 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3215 3215
Size: 6238
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4342 5243 5505 820
Size: 197 926 2399 901 262 1553
Fragments arranged by size:
2399 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SspI AAT'ATT
Cuts at: 5 52 4162 5
Size: 47 4110 2081
Fragments arranged by size:
4110 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6238
StyI C'CwwG_G
Cuts at: 513 1065 2151 2219 3677 513
Size: 552 1086 68 1458 3074
Fragments arranged by size:
3074 1458 1086 552 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2750 4265 4282 4435 4620 5065 325
Size: 1255 1170 1515 17 153 185 445 1498
Fragments arranged by size:
1515 1498 1255 1170 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3009 4484 6196
Size: 274 80 33 51 1443 1032 1475 1712
Cuts at: 6196 96
Size: 138
Fragments arranged by size:
1712 1475 1443 1032 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6198 6230 6198
Size: 32 6206
Tth111I GACn'n_nGTC
Cuts at: 1316 3194 1316
Size: 1878 4360
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2445 3302 2445
Size: 857 5381
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 4367 4367
Size: 6238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI