Restriction map for
pFN21AE2650
(Circular) (Six-base) MAPSORT of: pFN21AE2650.seq Check: 9712 from: 1 to: 7300
pFN21AE2650 7300 bp
With 182 enzymes: SgfI *
November 16, 2016 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5110 278
Size: 53 83 186 936 3574 2468
Fragments arranged by size:
3574 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3517 4643 1937
Size: 1580 1126 4594
Fragments arranged by size:
4594 1580 1126
Acc65I G'GTAC_C
Cuts at: 4621 4621
Size: 7300
AclI AA'CG_TT
Cuts at: 1563 5427 5800 1563
Size: 3864 373 3063
Fragments arranged by size:
3864 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3252 3306 5363 6747 7015 1632
Size: 1620 54 2057 1384 268 1917
Fragments arranged by size:
2057 1917 1620 1384 268 54
AflII C'TTAA_G
Cuts at: 820 1017 3761 820
Size: 197 2744 4359
Fragments arranged by size:
4359 2744 197
AflIII A'CryG_T
Cuts at: 1203 6199 7176 1203
Size: 4996 977 1327
Fragments arranged by size:
4996 1327 977
AgeI A'CCGG_T
Cuts at: 6131 6131
Size: 7300
AhdI GACnn_n'nnGTC
Cuts at: 6029 6029
Size: 7300
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7300
AlwNI CAG_nnn'CTG
Cuts at: 2102 5043 6615 2102
Size: 2941 1572 2787
Fragments arranged by size:
2941 2787 1572
ApaLI G'TGCA_C
Cuts at: 3590 5357 6513 3590
Size: 1767 1156 4377
Fragments arranged by size:
4377 1767 1156
ApoI r'AATT_y
Cuts at: 1493 2036 2184 2278 2398 2582 2621 2651 2828
Size: 543 148 94 120 184 39 30 177
Cuts at: 2828 2906 2997 3528 3730 3904 3943 4019 4122
Size: 78 91 531 202 174 39 76 103
Cuts at: 4122 4292 4300 4538 4609 4835 4987 7096 1493
Size: 170 8 238 71 226 152 2109 1697
Fragments arranged by size:
2109 1697 543 531 238 226 202 184
177 174 170 152 148 120 103 94
91 78 76 71 39 39 30 8
AseI AT'TA_AT
Cuts at: 160 1032 3870 5854 160
Size: 872 2838 1984 1606
Fragments arranged by size:
2838 1984 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7300
AvaI C'yCGr_G
Cuts at: 1870 1943 4625 1870
Size: 73 2682 4545
Fragments arranged by size:
4545 2682 73
BamHI G'GATC_C
Cuts at: 4630 4997 4630
Size: 367 6933
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4621 6076 618
Size: 325 217 627 2834 1455 1842
Fragments arranged by size:
2834 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3234 3464 4619 1391
Size: 1843 230 1155 4072
Fragments arranged by size:
4072 1843 1155 230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2785 4426 928
Size: 971 886 1641 3802
Fragments arranged by size:
3802 1641 971 886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5491 5525 1903
Size: 34 3554 34 3678
Fragments arranged by size:
3678 3554 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4024 5192 6402 4024
Size: 1168 1210 4922
Fragments arranged by size:
4922 1210 1168
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7300
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3238 4490 5911
Size: 107 122 71 631 605 1566 1252 1421
Cuts at: 5911 136
Size: 1525
Fragments arranged by size:
1566 1525 1421 1252 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2887 4091 7295 2887
Size: 1204 3204 2892
Fragments arranged by size:
3204 2892 1204
BlpI GC'TnA_GC
Cuts at: 3102 4717 3102
Size: 1615 5685
Bme1580I G_kGCm'C
Cuts at: 1790 3594 3627 5361 6517 1790
Size: 1804 33 1734 1156 2573
Fragments arranged by size:
2573 1804 1734 1156 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7300
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4592 5031 5989
Size: 416 53 83 399 24 3175 439 958
Cuts at: 5989 442
Size: 1753
Fragments arranged by size:
3175 1753 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7300
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2235 4287 2235
Size: 2052 5248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2275 2677 3066 3233 3263 5960 1239
Size: 1036 402 389 167 30 2697 2579
Fragments arranged by size:
2697 2579 1036 402 389 167 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3088 3117 3874 4238 4591 5425 6290 6588 3088
Size: 29 757 364 353 834 865 298 3800
Fragments arranged by size:
3800 865 834 757 364 353 298 29
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5963 882
Size: 617 4464 2219
Fragments arranged by size:
4464 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3515 5091 493
Size: 711 2311 1576 2702
Fragments arranged by size:
2702 2311 1576 711
BsaBI GATnn'nnATC
Cuts at: 2562 7294 2562
Size: 4732 2568
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5107 5489 6124 275
Size: 53 83 186 936 3574 382 635 1451
Fragments arranged by size:
3574 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2119 5733 6131 6405 6552 6963 2119
Size: 3614 398 274 147 411 2456
Fragments arranged by size:
3614 2456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7075
Fragments arranged by size:
7075 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2690 3125 3173 3230 3233 3236 3260
Size: 1033 471 435 48 57 3 3 24
Cuts at: 3260 3293 3317 3455 3908 4100 1186
Size: 33 24 138 453 192 4386
Fragments arranged by size:
4386 1033 471 453 435 192 138 57
48 33 24 24 3 3
BseYI C'CCAG_C
Cuts at: 6503 6999 6503
Size: 496 6804
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3779 1163
Size: 573 2043 4684
Fragments arranged by size:
4684 2043 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4750 5511 6539 1540
Size: 275 186 2749 761 1028 2301
Fragments arranged by size:
2749 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3464 3594 5361 5446 6517 3464
Size: 130 1767 85 1071 4247
Fragments arranged by size:
4247 1767 1071 130 85
BsmI GAATG_Cn'
Cuts at: 3718 4811 4904 3718
Size: 1093 93 6114
Fragments arranged by size:
6114 1093 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3234 3464 3594 3627 4619 5361 5446
Size: 399 1444 230 130 33 992 742 85
Cuts at: 5446 6517 1391
Size: 1071 2174
Fragments arranged by size:
2174 1444 1071 992 742 399 230 130
85 33
BspEI T'CCGG_A
Cuts at: 6963 6963
Size: 7300
BspHI T'CATG_A
Cuts at: 3700 5189 6919 3700
Size: 1489 1730 4081
Fragments arranged by size:
4081 1730 1489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4655 844
Size: 3811 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5187 1394
Size: 429 3364 3507
Fragments arranged by size:
3507 3364 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2575 2786 4435 5795 5969 65
Size: 1183 1327 211 1649 1360 174 1396
Fragments arranged by size:
1649 1396 1360 1327 1183 211 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5944 6131 1700
Size: 110 4134 187 2869
Fragments arranged by size:
4134 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2026 96
Size: 1930 5370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7300
BssSI C'ACGA_G
Cuts at: 5360 6372 5360
Size: 1012 6288
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7300
BstBI TT'CG_AA
Cuts at: 6104 6104
Size: 7300
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7300
BstYI r'GATC_y
Cuts at: 1972 2887 4091 4630 4997 5382 5399 6851 6937
Size: 915 1204 539 367 385 17 1452 86
Cuts at: 6937 6949 7295 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1204 915 539 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2268 3178 3205 3235 3292 2268
Size: 910 27 30 57 6276
Fragments arranged by size:
6276 910 57 30 27
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6748
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6561
Fragments arranged by size:
6561 683 56
BtsI GCAGTG_nn'
Cuts at: 4816 5610 5630 4816
Size: 794 20 6486
Fragments arranged by size:
6486 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7300
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7265
DraI TTT'AAA
Cuts at: 2133 2410 2685 2857 4604 2133
Size: 277 275 172 1747 4829
Fragments arranged by size:
4829 1747 277 275 172
DraIII CAC_nnn'GTG
Cuts at: 3586 3586
Size: 7300
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6307 809
Size: 507 4991 1802
Fragments arranged by size:
4991 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4747 5636 7265
Size: 54 1425 50 275 18 2917 889 1629
Cuts at: 7265 8
Size: 43
Fragments arranged by size:
2917 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4747 1537
Size: 275 2935 4090
Fragments arranged by size:
4090 2935 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3135 3165 3222 3255 3303 3603 4339 5230
Size: 1782 30 57 33 48 300 736 891
Cuts at: 5230 1353
Size: 3423
Fragments arranged by size:
3423 1782 891 736 300 57 48 33
30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5887 6261 6407 387
Size: 1064 138 4298 374 146 1280
Fragments arranged by size:
4298 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3462 3462
Size: 7300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2275 2677 3066 3233 3252 3263 3306
Size: 393 643 402 389 167 19 11 43
Cuts at: 3306 5363 5960 6747 7015 1239
Size: 2057 597 787 268 1524
Fragments arranged by size:
2057 1524 787 643 597 402 393 389
268 167 43 19 11
EcoRI G'AATT_C
Cuts at: 2278 2997 4019 4122 4300 4609 4987 2278
Size: 719 1022 103 178 309 378 4591
Fragments arranged by size:
4591 1022 719 378 309 178 103
FspI TGC'GCA
Cuts at: 5806 5806
Size: 7300
HaeII r_GCGC'y
Cuts at: 1678 6447 1678
Size: 4769 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2738 2770 3275 3307 3419
Size: 32 135 32 1166 32 505 32 112
Cuts at: 3419 3451 4076 4108 5941 5973 6015 6047 1373
Size: 32 625 32 1833 32 42 32 2626
Fragments arranged by size:
2626 1833 1166 625 505 135 112 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2094 4380 4644 4890 669
Size: 988 281 156 2286 264 246 3079
Fragments arranged by size:
3079 2286 988 281 264 246 156
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7300
HpaI GTT'AAC
Cuts at: 2094 4890 2094
Size: 2796 4504
KpnI G_GTAC'C
Cuts at: 4625 4625
Size: 7300
MfeI C'AATT_G
Cuts at: 4899 4899
Size: 7300
MluI A'CGCG_T
Cuts at: 7176 7176
Size: 7300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3255 6414 6598 1305
Size: 1950 3159 184 2007
Fragments arranged by size:
3159 2007 1950 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5478
Fragments arranged by size:
5478 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2857 3924 4286 5258 5617 5776
Size: 648 909 782 1067 362 972 359 159
Cuts at: 5776 518
Size: 2042
Fragments arranged by size:
2042 1067 972 909 782 648 362 359
159
MspA1I CmG'CkG
Cuts at: 1606 3620 4716 5393 6541 6786 1606
Size: 2014 1096 677 1148 245 2120
Fragments arranged by size:
2120 2014 1148 1096 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6748
NdeI CA'TA_TG
Cuts at: 387 4560 387
Size: 4173 3127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7300
NotI GC'GGCC_GC
Cuts at: 4747 4747
Size: 7300
NspI r_CATG'y
Cuts at: 1132 1229 4658 6203 1132
Size: 97 3429 1545 2229
Fragments arranged by size:
3429 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2488 1348
Size: 1140 6160
PciI A'CATG_T
Cuts at: 6199 6199
Size: 7300
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7300
PmeI GTTT'AAAC
Cuts at: 4604 4604
Size: 7300
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5369 5401 6882 6914 5369
Size: 32 1481 32 5755
Fragments arranged by size:
5755 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7300
PsiI TTA'TAA
Cuts at: 4870 4870
Size: 7300
PstI C_TGCA'G
Cuts at: 830 4652 830
Size: 3822 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7300
PvuII CAG'CTG
Cuts at: 3620 3620
Size: 7300
SacI G_AGCT'C
Cuts at: 3464 3464
Size: 7300
SalI G'TCGA_C
Cuts at: 1936 4642 1936
Size: 2706 4594
SapI GCTCTTCn'nnn_
Cuts at: 3222 3222
Size: 7300
SbfI CC_TGCA'GG
Cuts at: 4652 4652
Size: 7300
ScaI AGT'ACT
Cuts at: 2730 5548 7260 2730
Size: 2818 1712 2770
Fragments arranged by size:
2818 2770 1712
SexAI A'CCwGG_T
Cuts at: 3582 3582
Size: 7300
SfcI C'TryA_G
Cuts at: 826 1045 3420 4648 5783 6464 6655 826
Size: 219 2375 1228 1135 681 191 1471
Fragments arranged by size:
2375 1471 1228 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7300
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7300
SmaI CCC'GGG
Cuts at: 1872 4627 1872
Size: 2755 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3067 3132 3761 3853 4217 4570
Size: 197 926 1124 65 629 92 364 353
Cuts at: 4570 5404 6305 6567 820
Size: 834 901 262 1553
Fragments arranged by size:
1553 1124 926 901 834 629 364 353
262 197 92 65
SnaBI TAC'GTA
Cuts at: 493 5091 493
Size: 4598 2702
SpeI A'CTAG_T
Cuts at: 152 2021 152
Size: 1869 5431
SphI G_CATG'C
Cuts at: 1132 4658 1132
Size: 3526 3774
SspI AAT'ATT
Cuts at: 5 52 5224 5
Size: 47 5172 2081
Fragments arranged by size:
5172 2081 47
StuI AGG'CCT
Cuts at: 1517 3176 3290 1517
Size: 1659 114 5527
Fragments arranged by size:
5527 1659 114
StyI C'CwwG_G
Cuts at: 513 1065 4360 4739 513
Size: 552 3295 379 3074
Fragments arranged by size:
3295 3074 552 379
SwaI ATTT'AAAT
Cuts at: 2857 2857
Size: 7300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5327 5344 5497 5682 6127 325
Size: 1255 3747 17 153 185 445 1498
Fragments arranged by size:
3747 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2026 2728 2950
Size: 274 80 33 51 1443 49 702 222
Cuts at: 2950 5546 7258 96
Size: 2596 1712 138
Fragments arranged by size:
2596 1712 1443 702 274 222 138 80
51 49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4291 4323 7260 7292 4291
Size: 32 2937 32 4299
Fragments arranged by size:
4299 2937 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7300
XbaI T'CTAG_A
Cuts at: 4000 4094 4334 4636 4000
Size: 94 240 302 6664
Fragments arranged by size:
6664 302 240 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3394 3394
Size: 7300
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7300
XmaI C'CCGG_G
Cuts at: 1870 4625 1870
Size: 2755 4545
XmnI GAAnn'nnTTC
Cuts at: 5429 5429
Size: 7300
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5108 276
Size: 53 83 186 936 3574 2468
Fragments arranged by size:
3574 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI