Restriction map for
pFN21AE2643
(Circular) (Six-base) MAPSORT of: pFN21AE2643.seq Check: 1418 from: 1 to: 5020
pFN21AE2643 5020 bp
With 182 enzymes: SgfI *
October 21, 2016 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2048 2048
Size: 5020
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2830 278
Size: 53 83 186 936 1294 2468
Fragments arranged by size:
2468 1294 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2363 1937
Size: 426 4594
Acc65I G'GTAC_C
Cuts at: 2341 2341
Size: 5020
AclI AA'CG_TT
Cuts at: 1563 3147 3520 1563
Size: 1584 373 3063
Fragments arranged by size:
3063 1584 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 3083 4467 4735 1632
Size: 555 896 1384 268 1917
Fragments arranged by size:
1917 1384 896 555 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4823
AflIII A'CryG_T
Cuts at: 1203 3919 4896 1203
Size: 2716 977 1327
Fragments arranged by size:
2716 1327 977
AgeI A'CCGG_T
Cuts at: 3851 3851
Size: 5020
AhdI GACnn_n'nnGTC
Cuts at: 3749 3749
Size: 5020
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5020
AlwNI CAG_nnn'CTG
Cuts at: 2763 4335 2763
Size: 1572 3448
ApaLI G'TGCA_C
Cuts at: 3077 4233 3077
Size: 1156 3864
ApoI r'AATT_y
Cuts at: 1493 2267 2329 2555 2707 4816 1493
Size: 774 62 226 152 2109 1697
Fragments arranged by size:
2109 1697 774 226 152 62
AseI AT'TA_AT
Cuts at: 160 1032 2307 3574 160
Size: 872 1275 1267 1606
Fragments arranged by size:
1606 1275 1267 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5020
AvaI C'yCGr_G
Cuts at: 1870 1943 2345 1870
Size: 73 402 4545
Fragments arranged by size:
4545 402 73
BamHI G'GATC_C
Cuts at: 2350 2717 2350
Size: 367 4653
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2341 3796 618
Size: 325 217 627 554 1455 1842
Fragments arranged by size:
1842 1455 627 554 325 217
BanII G_rGCy'C
Cuts at: 1391 2339 1391
Size: 948 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3211 3245 1903
Size: 34 1274 34 3678
Fragments arranged by size:
3678 1274 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2912 4122 2912
Size: 1210 3810
BclI T'GATC_A
Cuts at: 1548 2115 1548
Size: 567 4453
BfrBI ATG'CAT
Cuts at: 2223 2223
Size: 5020
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3631 136
Size: 107 122 71 631 605 1959 1525
Fragments arranged by size:
1959 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5015 5015
Size: 5020
BlpI GC'TnA_GC
Cuts at: 2437 2437
Size: 5020
Bme1580I G_kGCm'C
Cuts at: 1790 3081 4237 1790
Size: 1291 1156 2573
Fragments arranged by size:
2573 1291 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5020
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2751 3709 442
Size: 416 53 83 399 24 1334 958 1753
Fragments arranged by size:
1753 1334 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 3680 1239
Size: 1056 1385 2579
Fragments arranged by size:
2579 1385 1056
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3145 4010 4308 3145
Size: 865 298 3857
Fragments arranged by size:
3857 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3683 882
Size: 617 2184 2219
Fragments arranged by size:
2219 2184 617
BsaAI yAC'GTr
Cuts at: 493 1204 2094 2811 493
Size: 711 890 717 2702
Fragments arranged by size:
2702 890 717 711
BsaBI GATnn'nnATC
Cuts at: 5014 5014
Size: 5020
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2827 3209 3844 275
Size: 53 83 186 936 1294 382 635 1451
Fragments arranged by size:
1451 1294 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3453 3851 4125 4272 4683 3453
Size: 398 274 147 411 3790
Fragments arranged by size:
3790 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4795
Fragments arranged by size:
4795 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5020
BseYI C'CCAG_C
Cuts at: 4223 4719 4223
Size: 496 4524
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4447
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2470 3231 4259 1540
Size: 275 186 469 761 1028 2301
Fragments arranged by size:
2301 1028 761 469 275 186
BsiHKAI G_wGCw'C
Cuts at: 3081 3166 4237 3081
Size: 85 1071 3864
Fragments arranged by size:
3864 1071 85
BsmI GAATG_Cn'
Cuts at: 2531 2624 2531
Size: 93 4927
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2339 3081 3166 4237 1391
Size: 399 549 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 549 399 85
BspEI T'CCGG_A
Cuts at: 4683 4683
Size: 5020
BspHI T'CATG_A
Cuts at: 2909 4639 2909
Size: 1730 3290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2048 2375 844
Size: 1204 327 3489
Fragments arranged by size:
3489 1204 327
BsrBI CCG'CTC
Cuts at: 1394 1823 2907 1394
Size: 429 1084 3507
Fragments arranged by size:
3507 1084 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3515 3689 65
Size: 1183 2267 174 1396
Fragments arranged by size:
2267 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3664 3851 1700
Size: 110 1854 187 2869
Fragments arranged by size:
2869 1854 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5020
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5020
BssSI C'ACGA_G
Cuts at: 3080 4092 3080
Size: 1012 4008
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5020
BstBI TT'CG_AA
Cuts at: 3824 3824
Size: 5020
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5020
BstYI r'GATC_y
Cuts at: 1972 2350 2717 3102 3119 4571 4657 4669 5015
Size: 378 367 385 17 1452 86 12 346
Cuts at: 5015 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 378 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3529
Fragments arranged by size:
3529 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4281
Fragments arranged by size:
4281 683 56
BtsI GCAGTG_nn'
Cuts at: 2536 3330 3350 2536
Size: 794 20 4206
Fragments arranged by size:
4206 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5020
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4985
DraI TTT'AAA
Cuts at: 2324 2324
Size: 5020
DraIII CAC_nnn'GTG
Cuts at: 2097 2097
Size: 5020
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4027 809
Size: 507 2711 1802
Fragments arranged by size:
2711 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2467 3356 4985
Size: 54 1425 50 275 18 637 889 1629
Cuts at: 4985 8
Size: 43
Fragments arranged by size:
1629 1425 889 637 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2467 1537
Size: 275 655 4090
Fragments arranged by size:
4090 655 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2950 1353
Size: 1597 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3607 3981 4127 387
Size: 1064 138 2018 374 146 1280
Fragments arranged by size:
2018 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2295 3083 3680 4467 4735 1239
Size: 393 555 108 788 597 787 268 1524
Fragments arranged by size:
1524 788 787 597 555 393 268 108
EcoRI G'AATT_C
Cuts at: 2329 2707 2329
Size: 378 4642
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 5020
FspI TGC'GCA
Cuts at: 3526 3526
Size: 5020
HaeII r_GCGC'y
Cuts at: 1678 4167 1678
Size: 2489 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3661 3693 3735 3767 1373
Size: 32 135 32 2089 32 42 32 2626
Fragments arranged by size:
2626 2089 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2080 2364 2610 669
Size: 988 281 142 284 246 3079
Fragments arranged by size:
3079 988 284 281 246 142
HindIII A'AGCT_T
Cuts at: 748 2291 748
Size: 1543 3477
HpaI GTT'AAC
Cuts at: 2610 2610
Size: 5020
KpnI G_GTAC'C
Cuts at: 2345 2345
Size: 5020
MfeI C'AATT_G
Cuts at: 2619 2619
Size: 5020
MluI A'CGCG_T
Cuts at: 4896 4896
Size: 5020
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 4134 4318 1305
Size: 873 1956 184 2007
Fragments arranged by size:
2007 1956 873 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3198
Fragments arranged by size:
3198 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2978 3337 3496 518
Size: 648 1812 359 159 2042
Fragments arranged by size:
2042 1812 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2436 3113 4261 4506 1606
Size: 830 677 1148 245 2120
Fragments arranged by size:
2120 1148 830 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3529
Fragments arranged by size:
3529 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5020
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5020
NotI GC'GGCC_GC
Cuts at: 2467 2467
Size: 5020
NsiI A_TGCA'T
Cuts at: 2225 2225
Size: 5020
NspI r_CATG'y
Cuts at: 1132 1229 2227 2378 3923 1132
Size: 97 998 151 1545 2229
Fragments arranged by size:
2229 1545 998 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5020
PciI A'CATG_T
Cuts at: 3919 3919
Size: 5020
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5020
PmeI GTTT'AAAC
Cuts at: 2324 2324
Size: 5020
PmlI CAC'GTG
Cuts at: 2094 2094
Size: 5020
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3089 3121 4602 4634 3089
Size: 32 1481 32 3475
Fragments arranged by size:
3475 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5020
PsiI TTA'TAA
Cuts at: 2590 2590
Size: 5020
PstI C_TGCA'G
Cuts at: 830 2059 2372 830
Size: 1229 313 3478
Fragments arranged by size:
3478 1229 313
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5020
SalI G'TCGA_C
Cuts at: 1936 2362 1936
Size: 426 4594
SbfI CC_TGCA'GG
Cuts at: 2372 2372
Size: 5020
ScaI AGT'ACT
Cuts at: 3268 4980 3268
Size: 1712 3308
SfcI C'TryA_G
Cuts at: 826 1045 2055 2368 3503 4184 4375 826
Size: 219 1010 313 1135 681 191 1471
Fragments arranged by size:
1471 1135 1010 681 313 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5020
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5020
SmaI CCC'GGG
Cuts at: 1872 2347 1872
Size: 475 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3124 4025 4287 820
Size: 197 926 1181 901 262 1553
Fragments arranged by size:
1553 1181 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2811 493
Size: 2318 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5020
SphI G_CATG'C
Cuts at: 1132 2227 2378 1132
Size: 1095 151 3774
Fragments arranged by size:
3774 1095 151
SspI AAT'ATT
Cuts at: 5 52 2944 5
Size: 47 2892 2081
Fragments arranged by size:
2892 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5020
StyI C'CwwG_G
Cuts at: 513 1065 2004 2046 2459 513
Size: 552 939 42 413 3074
Fragments arranged by size:
3074 939 552 413 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 3047 3064 3217 3402 3847 325
Size: 1255 672 795 17 153 185 445 1498
Fragments arranged by size:
1498 1255 795 672 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3266 4978 96
Size: 274 80 33 51 1443 1289 1712 138
Fragments arranged by size:
1712 1443 1289 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2244 2276 4980 5012 2244
Size: 32 2704 32 2252
Fragments arranged by size:
2704 2252 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5020
XbaI T'CTAG_A
Cuts at: 2356 2356
Size: 5020
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5020
XmaI C'CCGG_G
Cuts at: 1870 2345 1870
Size: 475 4545
XmnI GAAnn'nnTTC
Cuts at: 3149 3149
Size: 5020
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2828 276
Size: 53 83 186 936 1294 2468
Fragments arranged by size:
2468 1294 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI