Restriction map for
pFN21AE2630
(Circular) (Six-base) MAPSORT of: pFN21AE2630.seq Check: 3740 from: 1 to: 7162
pFN21AE2630 7162 bp
With 182 enzymes: SgfI *
June 3, 2018 17:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4972 278
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4505 1937
Size: 2568 4594
Acc65I G'GTAC_C
Cuts at: 4483 4483
Size: 7162
AclI AA'CG_TT
Cuts at: 1563 3077 5289 5662 1563
Size: 1514 2212 373 3063
Fragments arranged by size:
3063 2212 1514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2008 2579 2657 3809 5225 6609 6877 1632
Size: 376 571 78 1152 1416 1384 268 1917
Fragments arranged by size:
1917 1416 1384 1152 571 376 268 78
AfeI AGC'GCT
Cuts at: 2940 2940
Size: 7162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6965
AflIII A'CryG_T
Cuts at: 1203 2416 2883 6061 7038 1203
Size: 1213 467 3178 977 1327
Fragments arranged by size:
3178 1327 1213 977 467
AgeI A'CCGG_T
Cuts at: 5993 5993
Size: 7162
AhdI GACnn_n'nnGTC
Cuts at: 4375 5891 4375
Size: 1516 5646
AleI CACnn'nnGTG
Cuts at: 1166 2981 1166
Size: 1815 5347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2291 1184
Size: 1107 6055
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 7130
AlwNI CAG_nnn'CTG
Cuts at: 2717 4039 4318 4905 6477 2717
Size: 1322 279 587 1572 3402
Fragments arranged by size:
3402 1572 1322 587 279
ApaLI G'TGCA_C
Cuts at: 5219 6375 5219
Size: 1156 6006
ApoI r'AATT_y
Cuts at: 1493 3116 3552 4252 4471 4697 4849 6958 1493
Size: 1623 436 700 219 226 152 2109 1697
Fragments arranged by size:
2109 1697 1623 700 436 226 219 152
AseI AT'TA_AT
Cuts at: 160 1032 5716 160
Size: 872 4684 1606
Fragments arranged by size:
4684 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
AvaI C'yCGr_G
Cuts at: 1870 1943 4487 1870
Size: 73 2544 4545
Fragments arranged by size:
4545 2544 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4019 4052 4019
Size: 33 7129
BamHI G'GATC_C
Cuts at: 4492 4859 4492
Size: 367 6795
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2089 4483 5938 618
Size: 325 217 627 302 2394 1455 1842
Fragments arranged by size:
2394 1842 1455 627 325 302 217
BanII G_rGCy'C
Cuts at: 1391 4481 1391
Size: 3090 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2482 928
Size: 971 583 5608
Fragments arranged by size:
5608 971 583
BbvCI CC'TCA_GC
Cuts at: 3370 3987 3370
Size: 617 6545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5353 5387 1903
Size: 34 50 34 3332 34 3678
Fragments arranged by size:
3678 3332 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5054 6264 5054
Size: 1210 5952
BclI T'GATC_A
Cuts at: 1548 4083 1548
Size: 2535 4627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2909 5773
Size: 107 122 71 631 605 355 882 2864
Cuts at: 5773 136
Size: 1525
Fragments arranged by size:
2864 1525 882 631 605 355 122 107
71
BglII A'GATC_T
Cuts at: 7157 7157
Size: 7162
BlpI GC'TnA_GC
Cuts at: 4579 4579
Size: 7162
Bme1580I G_kGCm'C
Cuts at: 1790 4018 5223 6379 1790
Size: 2228 1205 1156 2573
Fragments arranged by size:
2573 2228 1205 1156
BmgBI CAC'GTC
Cuts at: 1318 2401 3940 1318
Size: 1083 1539 4540
Fragments arranged by size:
4540 1539 1083
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3797 4893 5851
Size: 416 53 83 399 24 2380 1096 958
Cuts at: 5851 442
Size: 1753
Fragments arranged by size:
2380 1753 1096 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 3223 3239 3519 5822 1239
Size: 841 1143 16 280 2303 2579
Fragments arranged by size:
2579 2303 1143 841 280 16
Bpu10I CC'TnA_GC
Cuts at: 3370 3705 3987 3370
Size: 335 282 6545
Fragments arranged by size:
6545 335 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5287 6152 6450 5287
Size: 865 298 5999
Fragments arranged by size:
5999 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2378 2981 5825 882
Size: 617 879 603 2844 2219
Fragments arranged by size:
2844 2219 879 617 603
BsaAI yAC'GTr
Cuts at: 493 1204 2380 2884 4953 493
Size: 711 1176 504 2069 2702
Fragments arranged by size:
2702 2069 1176 711 504
BsaBI GATnn'nnATC
Cuts at: 7156 7156
Size: 7162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4969 5351 5986 275
Size: 53 83 186 936 3436 382 635 1451
Fragments arranged by size:
3436 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5595 5993 6267 6414 6825 5595
Size: 398 274 147 411 5932
Fragments arranged by size:
5932 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6937
Fragments arranged by size:
6937 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2194 3598 1186
Size: 1008 1404 4750
Fragments arranged by size:
4750 1404 1008
BseYI C'CCAG_C
Cuts at: 2463 2780 2941 2969 4052 4189 6365 6861 2463
Size: 317 161 28 1083 137 2176 496 2764
Fragments arranged by size:
2764 2176 1083 496 317 161 137 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 3440 4025 4176 1163
Size: 573 561 1143 585 151 4149
Fragments arranged by size:
4149 1143 585 573 561 151
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4060 4612 5373 6401 1540
Size: 275 186 2059 552 761 1028 2301
Fragments arranged by size:
2301 2059 1028 761 552 275 186
BsiHKAI G_wGCw'C
Cuts at: 3657 5223 5308 6379 3657
Size: 1566 85 1071 4440
Fragments arranged by size:
4440 1566 1071 85
BsmI GAATG_Cn'
Cuts at: 4240 4673 4766 4240
Size: 433 93 6636
Fragments arranged by size:
6636 433 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3860 3860
Size: 7162
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3657 4018 4481 5223 5308 6379 1391
Size: 399 1867 361 463 742 85 1071 2174
Fragments arranged by size:
2174 1867 1071 742 463 399 361 85
BspEI T'CCGG_A
Cuts at: 6825 6825
Size: 7162
BspHI T'CATG_A
Cuts at: 5051 6781 5051
Size: 1730 5432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3412 4047 4517 844
Size: 2568 635 470 3489
Fragments arranged by size:
3489 2568 635 470
BsrBI CCG'CTC
Cuts at: 1394 1823 3694 5049 1394
Size: 429 1871 1355 3507
Fragments arranged by size:
3507 1871 1355 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3705 5657 5831 65
Size: 1183 2457 1952 174 1396
Fragments arranged by size:
2457 1952 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3878 5806 5993 1700
Size: 110 2068 1928 187 2869
Fragments arranged by size:
2869 2068 1928 187 110
BsrGI T'GTAC_A
Cuts at: 96 3324 96
Size: 3228 3934
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7162
BssSI C'ACGA_G
Cuts at: 5222 6234 5222
Size: 1012 6150
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4039 794
Size: 3245 3917
BstBI TT'CG_AA
Cuts at: 5966 5966
Size: 7162
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7162
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2528 3067 3557 3586 2248
Size: 280 539 490 29 5824
Fragments arranged by size:
5824 539 490 280 29
BstYI r'GATC_y
Cuts at: 1972 4063 4492 4859 5244 5261 6713 6799 6811
Size: 2091 429 367 385 17 1452 86 12
Cuts at: 6811 7157 1972
Size: 346 1977
Fragments arranged by size:
2091 1977 1452 429 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4323 4323
Size: 7162
BtgI C'CryG_G
Cuts at: 513 1065 2104 2317 3060 3999 513
Size: 552 1039 213 743 939 3676
Fragments arranged by size:
3676 1039 939 743 552 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6423
Fragments arranged by size:
6423 683 56
BtsI GCAGTG_nn'
Cuts at: 3390 3591 4218 4678 5472 5492 3390
Size: 201 627 460 794 20 5060
Fragments arranged by size:
5060 794 627 460 201 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7127
DraI TTT'AAA
Cuts at: 4466 4466
Size: 7162
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2132 6169 809
Size: 507 816 4037 1802
Fragments arranged by size:
4037 1802 816 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2686 3012
Size: 54 1425 50 275 18 327 529 326
Cuts at: 3012 3462 3714 3750 3880 4057 4609 5498 7127
Size: 450 252 36 130 177 552 889 1629
Cuts at: 7127 8
Size: 43
Fragments arranged by size:
1629 1425 889 552 529 450 327 326
275 252 177 130 54 50 43 36
18
EagI C'GGCC_G
Cuts at: 1537 1812 4057 4609 1537
Size: 275 2245 552 4090
Fragments arranged by size:
4090 2245 552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2954 3551 4179 5092 1353
Size: 1601 597 628 913 3423
Fragments arranged by size:
3423 1601 913 628 597
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5749 6123 6269 387
Size: 1064 138 4160 374 146 1280
Fragments arranged by size:
4160 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 2080 2579 2657 3223 3239 3519
Size: 393 376 72 499 78 566 16 280
Cuts at: 3519 3809 5225 5822 6609 6877 1239
Size: 290 1416 597 787 268 1524
Fragments arranged by size:
1524 1416 787 597 566 499 393 376
290 280 268 78 72 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2171 2171
Size: 7162
EcoO109I rG'GnC_Cy
Cuts at: 2668 3781 4138 2668
Size: 1113 357 5692
Fragments arranged by size:
5692 1113 357
EcoRI G'AATT_C
Cuts at: 4252 4471 4849 4252
Size: 219 378 6565
Fragments arranged by size:
6565 378 219
FspI TGC'GCA
Cuts at: 2227 5668 2227
Size: 3441 3721
HaeII r_GCGC'y
Cuts at: 1678 2087 2103 2557 2942 6309 1678
Size: 409 16 454 385 3367 2531
Fragments arranged by size:
3367 2531 454 409 385 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2472 2504 2716 2748 3833
Size: 32 135 32 900 32 212 32 1085
Cuts at: 3833 3865 4361 4393 5803 5835 5877 5909 1373
Size: 32 496 32 1410 32 42 32 2626
Fragments arranged by size:
2626 1410 1085 900 496 212 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2995 4506 4752 669
Size: 988 281 1057 1511 246 3079
Fragments arranged by size:
3079 1511 1057 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7162
HpaI GTT'AAC
Cuts at: 4752 4752
Size: 7162
KpnI G_GTAC'C
Cuts at: 4487 4487
Size: 7162
MfeI C'AATT_G
Cuts at: 4761 4761
Size: 7162
MluI A'CGCG_T
Cuts at: 7038 7038
Size: 7162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6276 6460 1305
Size: 4971 184 2007
Fragments arranged by size:
4971 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2159 3464 3716 10
Size: 54 1425 343 327 1305 252 3456
Fragments arranged by size:
3456 1425 1305 343 327 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2954 2981 3561 5120 5479 5638 518
Size: 648 1788 27 580 1559 359 159 2042
Fragments arranged by size:
2042 1788 1559 648 580 359 159 27
MspA1I CmG'CkG
Cuts at: 1606 2248 2780 2788 3028 4056 4578 5255 6403
Size: 642 532 8 240 1028 522 677 1148
Cuts at: 6403 6648 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1028 677 642 532 522 245
240 8
NcoI C'CATG_G
Cuts at: 513 1065 3060 513
Size: 552 1995 4615
Fragments arranged by size:
4615 1995 552
NdeI CA'TA_TG
Cuts at: 387 4168 387
Size: 3781 3381
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7162
NotI GC'GGCC_GC
Cuts at: 4609 4609
Size: 7162
NspI r_CATG'y
Cuts at: 1132 1229 2420 4520 6065 1132
Size: 97 1191 2100 1545 2229
Fragments arranged by size:
2229 2100 1545 1191 97
PasI CC'CwG_GG
Cuts at: 1348 2045 1348
Size: 697 6465
PciI A'CATG_T
Cuts at: 2416 6061 2416
Size: 3645 3517
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3061 3385 3460 1108
Size: 1953 324 75 4810
Fragments arranged by size:
4810 1953 324 75
PfoI T'CCnGG_A
Cuts at: 2823 2856 3108 3506 2823
Size: 33 252 398 6479
Fragments arranged by size:
6479 398 252 33
PmeI GTTT'AAAC
Cuts at: 4466 4466
Size: 7162
PmlI CAC'GTG
Cuts at: 2380 2380
Size: 7162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5231 5263 6744 6776 5231
Size: 32 1481 32 5617
Fragments arranged by size:
5617 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2668 3781 4138 2668
Size: 1113 357 5692
Fragments arranged by size:
5692 1113 357
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7162
PsiI TTA'TAA
Cuts at: 4732 4732
Size: 7162
PstI C_TGCA'G
Cuts at: 830 4514 830
Size: 3684 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7162
PvuII CAG'CTG
Cuts at: 2248 2780 2788 3028 2248
Size: 532 8 240 6382
Fragments arranged by size:
6382 532 240 8
SalI G'TCGA_C
Cuts at: 1936 4504 1936
Size: 2568 4594
SanDI GG'GwC_CC
Cuts at: 4138 4138
Size: 7162
SapI GCTCTTCn'nnn_
Cuts at: 2954 4179 2954
Size: 1225 5937
SbfI CC_TGCA'GG
Cuts at: 4514 4514
Size: 7162
ScaI AGT'ACT
Cuts at: 5410 7122 5410
Size: 1712 5450
SexAI A'CCwGG_T
Cuts at: 3963 3963
Size: 7162
SfcI C'TryA_G
Cuts at: 826 1045 2709 4284 4510 5645 6326 6517 826
Size: 219 1664 1575 226 1135 681 191 1471
Fragments arranged by size:
1664 1575 1471 1135 681 226 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7162
SmaI CCC'GGG
Cuts at: 1872 4489 1872
Size: 2617 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5266 6167 6429 820
Size: 197 926 3323 901 262 1553
Fragments arranged by size:
3323 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4953 493
Size: 4460 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7162
SphI G_CATG'C
Cuts at: 1132 4520 1132
Size: 3388 3774
SspI AAT'ATT
Cuts at: 5 52 4277 5086 5
Size: 47 4225 809 2081
Fragments arranged by size:
4225 2081 809 47
StuI AGG'CCT
Cuts at: 1517 2815 1517
Size: 1298 5864
StyI C'CwwG_G
Cuts at: 513 1065 2503 2683 3060 4601 513
Size: 552 1438 180 377 1541 3074
Fragments arranged by size:
3074 1541 1438 552 377 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3929 5189 5206 5359 5544 5989 325
Size: 1255 2349 1260 17 153 185 445 1498
Fragments arranged by size:
2349 1498 1260 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3324 5408 7120
Size: 274 80 33 51 1443 1347 2084 1712
Cuts at: 7120 96
Size: 138
Fragments arranged by size:
2084 1712 1443 1347 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7122 7154 7122
Size: 32 7130
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7162
XbaI T'CTAG_A
Cuts at: 4498 4498
Size: 7162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3774 3774
Size: 7162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7162
XmaI C'CCGG_G
Cuts at: 1870 4487 1870
Size: 2617 4545
XmnI GAAnn'nnTTC
Cuts at: 3077 5291 3077
Size: 2214 4948
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4970 276
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI