Restriction map for
pFN21AE2626
(Circular) (Six-base) MAPSORT of: pFN21AE2626.seq Check: 8865 from: 1 to: 5611
pFN21AE2626 5611 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2699 2699
Size: 5611
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2954 1937
Size: 1017 4594
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2031 2285 2457 2463 3674 5058 5326 1632
Size: 399 254 172 6 1211 1384 268 1917
Fragments arranged by size:
1917 1384 1211 399 268 254 172 6
AflII C'TTAA_G
Cuts at: 820 1017 2351 820
Size: 197 1334 4080
Fragments arranged by size:
4080 1334 197
AflIII A'CryG_T
Cuts at: 1203 4510 5487 1203
Size: 3307 977 1327
Fragments arranged by size:
3307 1327 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2190 1184
Size: 1006 4605
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2037 2069 2172 2204 2037
Size: 32 103 32 5444
Fragments arranged by size:
5444 103 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2145 3354 4926 2145
Size: 1209 1572 2830
Fragments arranged by size:
2830 1572 1209
ApaLI G'TGCA_C
Cuts at: 2712 3668 4824 2712
Size: 956 1156 3499
Fragments arranged by size:
3499 1156 956
ApoI r'AATT_y
Cuts at: 1493 2060 2315 2487 2584 2920 3146 3298 5407
Size: 567 255 172 97 336 226 152 2109
Cuts at: 5407 1493
Size: 1697
Fragments arranged by size:
2109 1697 567 336 255 226 172 152
97
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2936 1870
Size: 73 993 4545
Fragments arranged by size:
4545 993 73
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2932 4387 618
Size: 325 217 627 1145 1455 1842
Fragments arranged by size:
1842 1455 1145 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2930 1391
Size: 1539 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3802 3836 1903
Size: 34 1865 34 3678
Fragments arranged by size:
3678 1865 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3503 4713 3503
Size: 1210 4401
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4222 136
Size: 107 122 71 631 605 2550 1525
Fragments arranged by size:
2550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2878 5606 2878
Size: 2728 2883
BlpI GC'TnA_GC
Cuts at: 3028 3028
Size: 5611
Bme1580I G_kGCm'C
Cuts at: 1790 2716 3672 4828 1790
Size: 926 956 1156 2573
Fragments arranged by size:
2573 1156 956 926
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5611
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3342 4300 442
Size: 416 53 83 399 24 1925 958 1753
Fragments arranged by size:
1925 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2481 2481
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4271 1239
Size: 3032 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2478 3736 4601 4899 2478
Size: 1258 865 298 3190
Fragments arranged by size:
3190 1258 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4274 882
Size: 617 2775 2219
Fragments arranged by size:
2775 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3402 493
Size: 711 2198 2702
Fragments arranged by size:
2702 2198 711
BsaBI GATnn'nnATC
Cuts at: 2075 5605 2075
Size: 3530 2081
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3418 3800 4435 275
Size: 53 83 186 936 1885 382 635 1451
Fragments arranged by size:
1885 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4044 4442 4716 4863 5274 4044
Size: 398 274 147 411 4381
Fragments arranged by size:
4381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5386
Fragments arranged by size:
5386 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5611
BseYI C'CCAG_C
Cuts at: 2885 4814 5310 2885
Size: 1929 496 3186
Fragments arranged by size:
3186 1929 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2158 2769 1163
Size: 573 422 611 4005
Fragments arranged by size:
4005 611 573 422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3061 3822 4850 1540
Size: 275 186 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2716 3672 3757 4828 2716
Size: 956 85 1071 3499
Fragments arranged by size:
3499 1071 956 85
BsmI GAATG_Cn'
Cuts at: 3122 3215 3122
Size: 93 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2716 2930 3672 3757 4828 1391
Size: 399 926 214 742 85 1071 2174
Fragments arranged by size:
2174 1071 926 742 399 214 85
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 2966 844
Size: 1855 267 3489
Fragments arranged by size:
3489 1855 267
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 1394
Size: 429 1675 3507
Fragments arranged by size:
3507 1675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2610 4106 4280 65
Size: 1183 1362 1496 174 1396
Fragments arranged by size:
1496 1396 1362 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4255 4442 1700
Size: 110 2445 187 2869
Fragments arranged by size:
2869 2445 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5611
BssSI C'ACGA_G
Cuts at: 3671 4683 3671
Size: 1012 4599
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2777 794
Size: 1983 3628
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2278 2402 2878 2941 3308 3693 3710 5162
Size: 306 124 476 63 367 385 17 1452
Cuts at: 5162 5248 5260 5606 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 476 385 367 346 306 124
86 63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5059
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4872
Fragments arranged by size:
4872 683 56
BtsI GCAGTG_nn'
Cuts at: 2914 3127 3921 3941 2914
Size: 213 794 20 4584
Fragments arranged by size:
4584 794 213 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DraIII CAC_nnn'GTG
Cuts at: 2792 2792
Size: 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4618 809
Size: 507 3302 1802
Fragments arranged by size:
3302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3058 3947 5576
Size: 54 1425 50 275 18 1228 889 1629
Cuts at: 5576 8
Size: 43
Fragments arranged by size:
1629 1425 1228 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3058 1537
Size: 275 1246 4090
Fragments arranged by size:
4090 1246 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2261 2643 3541 1353
Size: 804 104 382 898 3423
Fragments arranged by size:
3423 898 804 382 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4198 4572 4718 387
Size: 1064 138 2609 374 146 1280
Fragments arranged by size:
2609 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2285 2457 2463 3674 4271 5058
Size: 393 399 254 172 6 1211 597 787
Cuts at: 5058 5326 1239
Size: 268 1524
Fragments arranged by size:
1524 1211 787 597 399 393 268 254
172 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2706 2706
Size: 5611
EcoO109I rG'GnC_Cy
Cuts at: 2738 2738
Size: 5611
EcoRI G'AATT_C
Cuts at: 2584 2920 3298 2584
Size: 336 378 4897
Fragments arranged by size:
4897 378 336
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 4758 1678
Size: 3080 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2193 2225 4252 4284 4326
Size: 32 135 32 621 32 2027 32 42
Cuts at: 4326 4358 1373
Size: 32 2626
Fragments arranged by size:
2626 2027 621 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2626 2955 3201 669
Size: 988 281 688 329 246 3079
Fragments arranged by size:
3079 988 688 329 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2347 4725 4909 1305
Size: 1042 2378 184 2007
Fragments arranged by size:
2378 2007 1042 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3789
Fragments arranged by size:
3789 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 3569 3928 4087 518
Size: 648 1006 1397 359 159 2042
Fragments arranged by size:
2042 1397 1006 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2783 2871 3027 3704 4852 5097 1606
Size: 1177 88 156 677 1148 245 2120
Fragments arranged by size:
2120 1177 1148 677 245 156 88
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5059
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2969 4514 1132
Size: 97 1740 1545 2229
Fragments arranged by size:
2229 1740 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 4510 4510
Size: 5611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5611
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2738 2738
Size: 5611
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2512 2963 830
Size: 1682 451 3478
Fragments arranged by size:
3478 1682 451
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
PvuII CAG'CTG
Cuts at: 2783 2871 2783
Size: 88 5523
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SapI GCTCTTCn'nnn_
Cuts at: 2157 2157
Size: 5611
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 2259 3859 5571 2259
Size: 1600 1712 2299
Fragments arranged by size:
2299 1712 1600
SfcI C'TryA_G
Cuts at: 826 1045 2340 2384 2508 2959 4094 4775 4966
Size: 219 1295 44 124 451 1135 681 191
Cuts at: 4966 826
Size: 1471
Fragments arranged by size:
1471 1295 1135 681 451 219 191 124
44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5611
SmaI CCC'GGG
Cuts at: 1872 2938 1872
Size: 1066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 2493 3715 4616 4878 820
Size: 197 926 408 142 1222 901 262 1553
Fragments arranged by size:
1553 1222 926 901 408 262 197 142
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2969 1132
Size: 1837 3774
SspI AAT'ATT
Cuts at: 5 52 2119 3535 5
Size: 47 2067 1416 2081
Fragments arranged by size:
2081 2067 1416 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5611
StyI C'CwwG_G
Cuts at: 513 1065 3050 513
Size: 552 1985 3074
Fragments arranged by size:
3074 1985 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3638 3655 3808 3993 4438 325
Size: 1255 2058 17 153 185 445 1498
Fragments arranged by size:
2058 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2257 3857 5569
Size: 274 80 33 51 1443 280 1600 1712
Cuts at: 5569 96
Size: 138
Fragments arranged by size:
1712 1600 1443 280 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5571 5603 5571
Size: 32 5579
Tth111I GACn'n_nGTC
Cuts at: 1316 2621 1316
Size: 1305 4306
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5611
XmaI C'CCGG_G
Cuts at: 1870 2936 1870
Size: 1066 4545
XmnI GAAnn'nnTTC
Cuts at: 3740 3740
Size: 5611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI