Restriction map for
pFN21AE2621
(Circular) (Six-base) MAPSORT of: pFN21AE2621.seq Check: 5915 from: 1 to: 6709
pFN21AE2621 6709 bp
With 182 enzymes: SgfI *
May 17, 2016 16:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2824 2824
Size: 6709
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4519 278
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4052 1937
Size: 2115 4594
Acc65I G'GTAC_C
Cuts at: 2886 3888 4030 2886
Size: 1002 142 5565
Fragments arranged by size:
5565 1002 142
AclI AA'CG_TT
Cuts at: 1563 4836 5209 1563
Size: 3273 373 3063
Fragments arranged by size:
3273 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3460 3756 4772 6156 6424 1632
Size: 1828 296 1016 1384 268 1917
Fragments arranged by size:
1917 1828 1384 1016 296 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6512
AflIII A'CryG_T
Cuts at: 1203 2430 5608 6585 1203
Size: 1227 3178 977 1327
Fragments arranged by size:
3178 1327 1227 977
AgeI A'CCGG_T
Cuts at: 3364 5540 3364
Size: 2176 4533
AhdI GACnn_n'nnGTC
Cuts at: 5438 5438
Size: 6709
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2281 1184
Size: 1097 5612
AlwNI CAG_nnn'CTG
Cuts at: 2163 2635 3064 3443 4452 6024 2163
Size: 472 429 379 1009 1572 2848
Fragments arranged by size:
2848 1572 1009 472 429 379
ApaI G_GGCC'C
Cuts at: 2458 3665 3849 2458
Size: 1207 184 5318
Fragments arranged by size:
5318 1207 184
ApaLI G'TGCA_C
Cuts at: 4766 5922 4766
Size: 1156 5553
ApoI r'AATT_y
Cuts at: 1493 3231 4018 4244 4396 6505 1493
Size: 1738 787 226 152 2109 1697
Fragments arranged by size:
2109 1738 1697 787 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5263 160
Size: 872 4231 1606
Fragments arranged by size:
4231 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 2695 2725 3192 3676 4034 1870
Size: 73 246 506 30 467 484 358 4545
Fragments arranged by size:
4545 506 484 467 358 246 73 30
BamHI G'GATC_C
Cuts at: 4039 4406 4039
Size: 367 6342
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2374 2396 2507 2799 2886
Size: 325 217 627 587 22 111 292 87
Cuts at: 2886 3888 4030 5485 618
Size: 1002 142 1455 1842
Fragments arranged by size:
1842 1455 1002 627 587 325 292 217
142 111 87 22
BanII G_rGCy'C
Cuts at: 1391 2112 2208 2458 3009 3665 3849 4028 1391
Size: 721 96 250 551 656 184 179 4072
Fragments arranged by size:
4072 721 656 551 250 184 179 96
BbeI G_GCGC'C
Cuts at: 2378 2400 2511 2803 2378
Size: 22 111 292 6284
Fragments arranged by size:
6284 292 111 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5738
BbvCI CC'TCA_GC
Cuts at: 3960 3960
Size: 6709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3110 3144 4900 4934 1903
Size: 34 1173 34 1756 34 3678
Fragments arranged by size:
3678 1756 1173 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4601 5811 4601
Size: 1210 5499
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6709
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2243 2366 2953
Size: 107 122 71 631 605 571 123 587
Cuts at: 2953 5320 136
Size: 2367 1525
Fragments arranged by size:
2367 1525 631 605 587 571 123 122
107 71
BglII A'GATC_T
Cuts at: 6704 6704
Size: 6709
BlpI GC'TnA_GC
Cuts at: 3085 4126 3085
Size: 1041 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2458 3665 3849 4770 5926 1790
Size: 668 1207 184 921 1156 2573
Fragments arranged by size:
2573 1207 1156 921 668 184
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6709
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2270 2618 4440
Size: 416 53 83 399 24 853 348 1822
Cuts at: 4440 5398 442
Size: 958 1753
Fragments arranged by size:
1822 1753 958 853 416 399 348 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 6709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2049 2555 2600 3812 3850 5369 1239
Size: 810 506 45 1212 38 1519 2579
Fragments arranged by size:
2579 1519 1212 810 506 45 38
Bpu10I CC'TnA_GC
Cuts at: 2310 3960 2310
Size: 1650 5059
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3295 4834 5699 5997 3295
Size: 1539 865 298 4007
Fragments arranged by size:
4007 1539 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5372 882
Size: 617 3873 2219
Fragments arranged by size:
3873 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2593 4500 493
Size: 711 1389 1907 2702
Fragments arranged by size:
2702 1907 1389 711
BsaBI GATnn'nnATC
Cuts at: 6703 6703
Size: 6709
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2195 2375 2397 2508
Size: 53 83 186 936 662 180 22 111
Cuts at: 2508 2586 2800 4516 4898 5533 275
Size: 78 214 1716 382 635 1451
Fragments arranged by size:
1716 1451 936 662 635 382 214 186
180 111 83 78 53 22
BsaWI w'CCGG_w
Cuts at: 2134 3364 5142 5540 5814 5961 6372 2134
Size: 1230 1778 398 274 147 411 2471
Fragments arranged by size:
2471 1778 1230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 2417 2447 3673
Size: 30 165 30 792 30 192 30 1226
Cuts at: 3673 3703 3917 3947 1178
Size: 30 214 30 3940
Fragments arranged by size:
3940 1226 792 214 192 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3017 3197 3227 3557 3694 3793 3941 1186
Size: 1831 180 30 330 137 99 148 3954
Fragments arranged by size:
3954 1831 330 180 148 137 99 30
BseYI C'CCAG_C
Cuts at: 2619 3483 3519 3825 3912 3953 5912 6408 2619
Size: 864 36 306 87 41 1959 496 2920
Fragments arranged by size:
2920 1959 864 496 306 87 41 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2118 2410 2490 2620 1163
Size: 573 382 292 80 130 5252
Fragments arranged by size:
5252 573 382 292 130 80
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2521 2662 4159 4920 5948 1540
Size: 275 186 520 141 1497 761 1028 2301
Fragments arranged by size:
2301 1497 1028 761 520 275 186 141
BsiHKAI G_wGCw'C
Cuts at: 2112 2260 2430 2783 2790 2910 3009 3468 3701
Size: 148 170 353 7 120 99 459 233
Cuts at: 3701 4770 4855 5926 2112
Size: 1069 85 1071 2895
Fragments arranged by size:
2895 1071 1069 459 353 233 170 148
120 99 85 7
BsmI GAATG_Cn'
Cuts at: 4220 4313 4220
Size: 93 6616
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2208 2260 2430 2458 2783 2790
Size: 399 322 96 52 170 28 325 7
Cuts at: 2790 2910 3009 3468 3665 3701 3849 4028 4770
Size: 120 99 459 197 36 148 179 742
Cuts at: 4770 4855 5926 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 459 399 325 322 197
179 170 148 120 99 96 85 52
36 28 7
BspEI T'CCGG_A
Cuts at: 6372 6372
Size: 6709
BspHI T'CATG_A
Cuts at: 4598 6328 4598
Size: 1730 4979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2824 3549 4064 844
Size: 1980 725 515 3489
Fragments arranged by size:
3489 1980 725 515
BsrBI CCG'CTC
Cuts at: 1394 1823 2128 4596 1394
Size: 429 305 2468 3507
Fragments arranged by size:
3507 2468 429 305
BsrDI GCAATG_nn'
Cuts at: 65 1248 3001 5204 5378 65
Size: 1183 1753 2203 174 1396
Fragments arranged by size:
2203 1753 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2516 3364 5353 5540 1700
Size: 110 706 848 1989 187 2869
Fragments arranged by size:
2869 1989 848 706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6709
BssHII G'CGCG_C
Cuts at: 1925 2481 1925
Size: 556 6153
BssSI C'ACGA_G
Cuts at: 4769 5781 4769
Size: 1012 5697
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2163 2357 2653 794
Size: 1369 194 296 4850
Fragments arranged by size:
4850 1369 296 194
BstBI TT'CG_AA
Cuts at: 5513 5513
Size: 6709
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6709
BstXI CCAn_nnnn'nTGG
Cuts at: 3226 3226
Size: 6709
BstYI r'GATC_y
Cuts at: 1972 4039 4406 4791 4808 6260 6346 6358 6704
Size: 2067 367 385 17 1452 86 12 346
Cuts at: 6704 1972
Size: 1977
Fragments arranged by size:
2067 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3429 3429
Size: 6709
BtgI C'CryG_G
Cuts at: 513 1065 2662 3749 513
Size: 552 1597 1087 3473
Fragments arranged by size:
3473 1597 1087 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2577 487
Size: 683 56 1351 4619
Fragments arranged by size:
4619 1351 683 56
BtsI GCAGTG_nn'
Cuts at: 2101 3634 4225 5019 5039 2101
Size: 1533 591 794 20 3771
Fragments arranged by size:
3771 1533 794 591 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6709
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6674
DraI TTT'AAA
Cuts at: 4013 4013
Size: 6709
DraIII CAC_nnn'GTG
Cuts at: 2596 2871 2596
Size: 275 6434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5716 809
Size: 507 4400 1802
Fragments arranged by size:
4400 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2367 2518 2659
Size: 54 1425 50 275 18 537 151 141
Cuts at: 2659 2752 3816 4156 5045 6674 8
Size: 93 1064 340 889 1629 43
Fragments arranged by size:
1629 1425 1064 889 537 340 275 151
141 93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2518 2659 4156 1537
Size: 275 706 141 1497 4090
Fragments arranged by size:
4090 1497 706 275 141
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 3588 3709 4639 1353
Size: 665 1570 121 930 3423
Fragments arranged by size:
3423 1570 930 665 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2291 5296 5670 5816 387
Size: 1064 138 702 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 702 374 146 138
EcoICRI GAG'CTC
Cuts at: 2110 3007 2110
Size: 897 5812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2555 2600 3460 3756 3812 3850
Size: 393 417 506 45 860 296 56 38
Cuts at: 3850 4772 5369 6156 6424 1239
Size: 922 597 787 268 1524
Fragments arranged by size:
1524 922 860 787 597 506 417 393
296 268 56 45 38
EcoO109I rG'GnC_Cy
Cuts at: 2575 2632 3076 3418 3662 3845 3846 3948 2575
Size: 57 444 342 244 183 1 102 5336
Fragments arranged by size:
5336 444 342 244 183 102 57 1
EcoRI G'AATT_C
Cuts at: 3231 4018 4396 3231
Size: 787 378 5544
Fragments arranged by size:
5544 787 378
FspI TGC'GCA
Cuts at: 5215 5215
Size: 6709
HaeII r_GCGC'y
Cuts at: 1678 2222 2378 2400 2511 2803 2817 3483 5856
Size: 544 156 22 111 292 14 666 2373
Cuts at: 5856 1678
Size: 2531
Fragments arranged by size:
2531 2373 666 544 292 156 111 22
14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 2777 2809 3425
Size: 32 135 32 965 32 208 32 616
Cuts at: 3425 3431 3457 3463 3638 3670 3848 3880 5350
Size: 6 26 6 175 32 178 32 1470
Cuts at: 5350 5382 5424 5456 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1470 965 616 208 178 175 135
42 32 32 32 32 32 32 32
32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4053 4299 669
Size: 988 281 2115 246 3079
Fragments arranged by size:
3079 2115 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2706 748
Size: 1958 4751
HpaI GTT'AAC
Cuts at: 4299 4299
Size: 6709
KasI G'GCGC_C
Cuts at: 2374 2396 2507 2799 2374
Size: 22 111 292 6284
Fragments arranged by size:
6284 292 111 22
KpnI G_GTAC'C
Cuts at: 2890 3892 4034 2890
Size: 1002 142 5565
Fragments arranged by size:
5565 1002 142
MfeI C'AATT_G
Cuts at: 4308 4308
Size: 6709
MluI A'CGCG_T
Cuts at: 2430 6585 2430
Size: 4155 2554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3042 3069 5823 6007 1305
Size: 1737 27 2754 184 2007
Fragments arranged by size:
2754 2007 1737 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3818 10
Size: 54 1425 343 1986 2901
Fragments arranged by size:
2901 1986 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4667 5026 5185 518
Size: 648 3501 359 159 2042
Fragments arranged by size:
3501 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2163 2169 2394 2664 2823 3523 4125 4802
Size: 557 6 225 270 159 700 602 677
Cuts at: 4802 5950 6195 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 700 677 602 557 270 245
225 159 6
NaeI GCC'GGC
Cuts at: 2518 2518
Size: 6709
NarI GG'CG_CC
Cuts at: 2375 2397 2508 2800 2375
Size: 22 111 292 6284
Fragments arranged by size:
6284 292 111 22
NcoI C'CATG_G
Cuts at: 513 1065 3749 513
Size: 552 2684 3473
Fragments arranged by size:
3473 2684 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6709
NgoMIV G'CCGG_C
Cuts at: 2516 2516
Size: 6709
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6709
NotI GC'GGCC_GC
Cuts at: 4156 4156
Size: 6709
NspI r_CATG'y
Cuts at: 1132 1229 3605 4067 5612 1132
Size: 97 2376 462 1545 2229
Fragments arranged by size:
2376 2229 1545 462 97
PasI CC'CwG_GG
Cuts at: 1348 3658 1348
Size: 2310 4399
PciI A'CATG_T
Cuts at: 5608 5608
Size: 6709
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2920 3064 1108
Size: 1812 144 4753
Fragments arranged by size:
4753 1812 144
PfoI T'CCnGG_A
Cuts at: 3092 3789 3092
Size: 697 6012
PmeI GTTT'AAAC
Cuts at: 4013 4013
Size: 6709
PmlI CAC'GTG
Cuts at: 2593 2593
Size: 6709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4778 4810 6291 6323 4778
Size: 32 1481 32 5164
Fragments arranged by size:
5164 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2575 3076 3948 2575
Size: 501 872 5336
Fragments arranged by size:
5336 872 501
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6709
PsiI TTA'TAA
Cuts at: 4279 4279
Size: 6709
PspOMI G'GGCC_C
Cuts at: 2454 3661 3845 2454
Size: 1207 184 5318
Fragments arranged by size:
5318 1207 184
PspXI vC'TCGA_Gb
Cuts at: 2189 2189
Size: 6709
PstI C_TGCA'G
Cuts at: 830 2168 2332 3056 4061 830
Size: 1338 164 724 1005 3478
Fragments arranged by size:
3478 1338 1005 724 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6709
PvuII CAG'CTG
Cuts at: 2163 2163
Size: 6709
RsrII CG'GwC_CG
Cuts at: 2137 2137
Size: 6709
SacI G_AGCT'C
Cuts at: 2112 3009 2112
Size: 897 5812
SacII CC_GC'GG
Cuts at: 2665 2665
Size: 6709
SalI G'TCGA_C
Cuts at: 1936 4051 1936
Size: 2115 4594
SanDI GG'GwC_CC
Cuts at: 3948 3948
Size: 6709
SapI GCTCTTCn'nnn_
Cuts at: 2018 2018
Size: 6709
SbfI CC_TGCA'GG
Cuts at: 4061 4061
Size: 6709
ScaI AGT'ACT
Cuts at: 4957 6669 4957
Size: 1712 4997
SexAI A'CCwGG_T
Cuts at: 2682 2682
Size: 6709
SfcI C'TryA_G
Cuts at: 826 1045 2164 2328 3052 3150 4057 5192 5873
Size: 219 1119 164 724 98 907 1135 681
Cuts at: 5873 6064 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1119 907 724 681 219 191
164 98
SfoI GGC'GCC
Cuts at: 2376 2398 2509 2801 2376
Size: 22 111 292 6284
Fragments arranged by size:
6284 292 111 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6709
SmaI CCC'GGG
Cuts at: 1872 2727 4036 1872
Size: 855 1309 4545
Fragments arranged by size:
4545 1309 855
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 3274 4813 5714 5976 820
Size: 197 926 246 1085 1539 901 262 1553
Fragments arranged by size:
1553 1539 1085 926 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 4500 493
Size: 4007 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6709
SphI G_CATG'C
Cuts at: 1132 3605 4067 1132
Size: 2473 462 3774
Fragments arranged by size:
3774 2473 462
SspI AAT'ATT
Cuts at: 5 52 4633 5
Size: 47 4581 2081
Fragments arranged by size:
4581 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6709
StyI C'CwwG_G
Cuts at: 513 1065 2901 2919 2949 3241 3576 3749 4148
Size: 552 1836 18 30 292 335 173 399
Cuts at: 4148 513
Size: 3074
Fragments arranged by size:
3074 1836 552 399 335 292 173 30
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3839 4736 4753 4906 5091 5536 325
Size: 1255 2259 897 17 153 185 445 1498
Fragments arranged by size:
2259 1498 1255 897 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2982 4955 6667
Size: 274 80 33 51 1443 1005 1973 1712
Cuts at: 6667 96
Size: 138
Fragments arranged by size:
1973 1712 1443 1005 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2195 2227 2417 2449 2954 2986 3095 3127 3673
Size: 32 190 32 505 32 109 32 546
Cuts at: 3673 3705 6669 6701 2195
Size: 32 2964 32 2203
Fragments arranged by size:
2964 2203 546 505 190 109 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6709
XbaI T'CTAG_A
Cuts at: 4045 4045
Size: 6709
XhoI C'TCGA_G
Cuts at: 1943 2189 1943
Size: 246 6463
XmaI C'CCGG_G
Cuts at: 1870 2725 4034 1870
Size: 855 1309 4545
Fragments arranged by size:
4545 1309 855
XmnI GAAnn'nnTTC
Cuts at: 4838 4838
Size: 6709
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4517 276
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PsrI SfiI SrfI SwaI XcmI