Restriction map for
pFN21AE2616
(Circular) (Six-base) MAPSORT of: pFN21AE2616.seq Check: 5967 from: 1 to: 6055
pFN21AE2616 6055 bp
With 182 enzymes: SgfI *
April 1, 2016 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2695 2695
Size: 6055
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2427 3865 278
Size: 53 83 186 936 891 1438 2468
Fragments arranged by size:
2468 1438 936 891 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2912 4118 5502 5770 1632
Size: 1280 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1280 1206 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 2916 4954 5931 1203
Size: 1713 2038 977 1327
Fragments arranged by size:
2038 1713 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 2182 4784 2182
Size: 2602 3453
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 6023
AlwNI CAG_nnn'CTG
Cuts at: 2375 2640 2710 2800 3010 3345 3798 5370 2375
Size: 265 70 90 210 335 453 1572 3060
Fragments arranged by size:
3060 1572 453 335 265 210 90 70
ApaI G_GGCC'C
Cuts at: 3198 3206 3354 3198
Size: 8 148 5899
Fragments arranged by size:
5899 148 8
ApaLI G'TGCA_C
Cuts at: 3107 4112 5268 3107
Size: 1005 1156 3894
Fragments arranged by size:
3894 1156 1005
ApoI r'AATT_y
Cuts at: 1493 2600 3364 3590 3742 5851 1493
Size: 1107 764 226 152 2109 1697
Fragments arranged by size:
2109 1697 1107 764 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2453 3206 3286 3380 1870
Size: 73 510 753 80 94 4545
Fragments arranged by size:
4545 753 510 94 80 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2191 2569 2879 2998
Size: 325 217 627 244 160 378 310 119
Cuts at: 2998 3376 4831 618
Size: 378 1455 1842
Fragments arranged by size:
1842 1455 627 378 378 325 310 244
217 160 119
BanII G_rGCy'C
Cuts at: 1391 2454 2813 2858 3198 3206 3354 3374 1391
Size: 1063 359 45 340 8 148 20 4072
Fragments arranged by size:
4072 1063 359 340 148 45 20 8
BbeI G_GCGC'C
Cuts at: 2195 2195
Size: 6055
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5084
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4246 4280 1903
Size: 34 50 34 2225 34 3678
Fragments arranged by size:
3678 2225 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2384 4666 136
Size: 107 122 71 631 605 712 2282 1525
Fragments arranged by size:
2282 1525 712 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 2060 3472 2060
Size: 1412 4643
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2574 3111 3198 3206 3354 4116 5272
Size: 349 435 537 87 8 148 762 1156
Cuts at: 5272 1790
Size: 2573
Fragments arranged by size:
2573 1156 762 537 435 349 148 87
8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 3268 3786
Size: 416 53 83 399 24 624 1227 518
Cuts at: 3786 4744 442
Size: 958 1753
Fragments arranged by size:
1753 1227 958 624 518 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2469 2708 3002 4715 1239
Size: 1230 239 294 1713 2579
Fragments arranged by size:
2579 1713 1230 294 239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4180 5045 5343 4180
Size: 865 298 4892
Fragments arranged by size:
4892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3289 4718 882
Size: 617 1790 1429 2219
Fragments arranged by size:
2219 1790 1429 617
BsaAI yAC'GTr
Cuts at: 493 1204 2416 2919 3846 493
Size: 711 1212 503 927 2702
Fragments arranged by size:
2702 1212 927 711 503
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2192 2424 3862 4244
Size: 53 83 186 936 659 232 1438 382
Cuts at: 4244 4879 275
Size: 635 1451
Fragments arranged by size:
1451 1438 936 659 635 382 232 186
83 53
BsaWI w'CCGG_w
Cuts at: 4488 4886 5160 5307 5718 4488
Size: 398 274 147 411 4825
Fragments arranged by size:
4825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2795 2825 1178
Size: 30 165 30 1392 30 4408
Fragments arranged by size:
4408 1392 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6055
BseYI C'CCAG_C
Cuts at: 2162 2710 3329 5258 5754 2162
Size: 548 619 1929 496 2463
Fragments arranged by size:
2463 1929 619 548 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3283 1163
Size: 573 1547 3935
Fragments arranged by size:
3935 1547 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2761 3505 4266 5294 1540
Size: 275 186 760 744 761 1028 2301
Fragments arranged by size:
2301 1028 761 760 744 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2415 2454 2813 3111 4116 4201 5272 2124
Size: 291 39 359 298 1005 85 1071 2907
Fragments arranged by size:
2907 1071 1005 359 298 291 85 39
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
BsmBI CGTCTCn'nnnn_
Cuts at: 2901 2901
Size: 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2139 2415 2454 2574 2813 2858
Size: 399 334 15 276 39 120 239 45
Cuts at: 2858 3111 3198 3206 3354 3374 4116 4201 5272
Size: 253 87 8 148 20 742 85 1071
Cuts at: 5272 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 334 276 253 239
148 120 87 85 45 39 20 15
8
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2695 3410 844
Size: 1851 715 3489
Fragments arranged by size:
3489 1851 715
BsrBI CCG'CTC
Cuts at: 1394 1823 2296 3942 1394
Size: 429 473 1646 3507
Fragments arranged by size:
3507 1646 473 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2188 2549 2658 2718 2882 3082 4699
Size: 110 378 361 109 60 164 200 1617
Cuts at: 4699 4886 1700
Size: 187 2869
Fragments arranged by size:
2869 1617 378 361 200 187 164 110
109 60
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 3535
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 2447 4115 5127 2447
Size: 1668 1012 3375
Fragments arranged by size:
3375 1668 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 2018 1184
Size: 834 5221
BstYI r'GATC_y
Cuts at: 1972 2955 3385 3752 4137 4154 5606 5692 5704
Size: 983 430 367 385 17 1452 86 12
Cuts at: 5704 6050 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 983 430 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2040 2070 3198 3223 3279 513
Size: 552 975 30 1128 25 56 3289
Fragments arranged by size:
3289 1128 975 552 56 30 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 2373 3240 3295 3571 4365 4385 2373
Size: 867 55 276 794 20 4043
Fragments arranged by size:
4043 867 794 276 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DraIII CAC_nnn'GTG
Cuts at: 3235 3235
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2265 2752
Size: 54 1425 50 275 18 270 165 487
Cuts at: 2752 3226 3502 4391 6020 8
Size: 474 276 889 1629 43
Fragments arranged by size:
1629 1425 889 487 474 276 275 270
165 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3985 1353
Size: 2632 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2803 3294 4642 5016 5162 387
Size: 1064 138 1214 491 1348 374 146 1280
Fragments arranged by size:
1348 1280 1214 1064 491 374 146 138
EcoICRI GAG'CTC
Cuts at: 2452 2811 2452
Size: 359 5696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 2708 2912 3002 4118 4715 5502
Size: 393 837 239 204 90 1116 597 787
Cuts at: 5502 5770 1239
Size: 268 1524
Fragments arranged by size:
1524 1116 837 787 597 393 268 239
204 90
EcoO109I rG'GnC_Cy
Cuts at: 2797 3194 3203 3291 3342 3350 2797
Size: 397 9 88 51 8 5502
Fragments arranged by size:
5502 397 88 51 9 8
EcoRI G'AATT_C
Cuts at: 2600 3364 3742 2600
Size: 764 378 4913
Fragments arranged by size:
4913 764 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 6055
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 2195 3273 5202 1678
Size: 517 1078 1929 2531
Fragments arranged by size:
2531 1929 1078 517
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 3040 3072 4696
Size: 32 135 32 596 32 840 32 1624
Cuts at: 4696 4728 4770 4802 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1624 840 596 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3139 3399 3645 669
Size: 988 281 1201 260 246 3079
Fragments arranged by size:
3079 1201 988 281 260 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KasI G'GCGC_C
Cuts at: 2191 2191
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 5169 5353 1305
Size: 927 2937 184 2007
Fragments arranged by size:
2937 2007 927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3228 10
Size: 54 1425 343 1396 2837
Fragments arranged by size:
2837 1425 1396 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2504 4013 4372 4531 518
Size: 648 1338 1509 359 159 2042
Fragments arranged by size:
2042 1509 1338 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2640 3145 3471 4148 5296 5541 1606
Size: 1034 505 326 677 1148 245 2120
Fragments arranged by size:
2120 1148 1034 677 505 326 245
NaeI GCC'GGC
Cuts at: 2190 2190
Size: 6055
NarI GG'CG_CC
Cuts at: 2192 2192
Size: 6055
NcoI C'CATG_G
Cuts at: 513 1065 2070 3223 3279 513
Size: 552 1005 1153 56 3289
Fragments arranged by size:
3289 1153 1005 552 56
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NgoMIV G'CCGG_C
Cuts at: 2188 2188
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 2334 3413 4958 1132
Size: 97 1105 1079 1545 2229
Fragments arranged by size:
2229 1545 1105 1079 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3148 3280 1108
Size: 2040 132 3883
Fragments arranged by size:
3883 2040 132
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PmlI CAC'GTG
Cuts at: 2416 2416
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 4124 4156 5637 5669 2795
Size: 32 1297 32 1481 32 3181
Fragments arranged by size:
3181 1481 1297 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3291 3342 3291
Size: 51 6004
PshAI GACnn'nnGTC
Cuts at: 1613 2423 1613
Size: 810 5245
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 3194 3202 3350 3194
Size: 8 148 5899
Fragments arranged by size:
5899 148 8
PstI C_TGCA'G
Cuts at: 830 3407 830
Size: 2577 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2640 3145 2640
Size: 505 5550
SacI G_AGCT'C
Cuts at: 2454 2813 2454
Size: 359 5696
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SanDI GG'GwC_CC
Cuts at: 3291 3291
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 3044 3062 3403 4538 5219 5410 826
Size: 219 1999 18 341 1135 681 191 1471
Fragments arranged by size:
1999 1471 1135 681 341 219 191 18
SfoI GGC'GCC
Cuts at: 2193 2193
Size: 6055
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 2188 1700
Size: 488 5567
SmaI CCC'GGG
Cuts at: 1872 3208 3382 1872
Size: 1336 174 4545
Fragments arranged by size:
4545 1336 174
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4159 5060 5322 820
Size: 197 926 2216 901 262 1553
Fragments arranged by size:
2216 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2070 2875 3223 3279 3494 513
Size: 552 1005 805 348 56 215 3074
Fragments arranged by size:
3074 1005 805 552 348 215 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2156 2435 2775 3199 4082 4099 4252
Size: 1255 576 279 340 424 883 17 153
Cuts at: 4252 4437 4882 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 883 576 445 424 340 279
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 4301 6013
Size: 274 80 33 51 1443 639 1685 1712
Cuts at: 6013 96
Size: 138
Fragments arranged by size:
1712 1685 1443 639 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6015 6047 6015
Size: 32 6023
Tth111I GACn'n_nGTC
Cuts at: 1316 2543 1316
Size: 1227 4828
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3206 3380 1870
Size: 1336 174 4545
Fragments arranged by size:
4545 1336 174
XmnI GAAnn'nnTTC
Cuts at: 4184 4184
Size: 6055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2425 3863 276
Size: 53 83 186 936 891 1438 2468
Fragments arranged by size:
2468 1438 936 891 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
NruI NsiI PacI PfoI PspXI PsrI RsrII SacII
SapI SexAI SfiI SrfI SwaI XcmI