Restriction map for
pFN21AE2614
(Circular) (Six-base) MAPSORT of: pFN21AE2614.seq Check: 4457 from: 1 to: 6295
pFN21AE2614 6295 bp
With 182 enzymes: SgfI *
March 25, 2016 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3261 3261
Size: 6295
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2880 4105 278
Size: 53 83 186 936 1344 1225 2468
Fragments arranged by size:
2468 1344 1225 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3309 3638 1937
Size: 1372 329 4594
Fragments arranged by size:
4594 1372 329
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3004 4358 5742 6010 1632
Size: 1372 1354 1384 268 1917
Fragments arranged by size:
1917 1384 1372 1354 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 2466 3477 5194 6171 1203
Size: 1263 1011 1717 977 1327
Fragments arranged by size:
1717 1327 1263 1011 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 2276 1166
Size: 1110 5185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3269 3569 1184
Size: 2085 300 3910
Fragments arranged by size:
3910 2085 300
AlwNI CAG_nnn'CTG
Cuts at: 2460 4038 5610 2460
Size: 1578 1572 3145
Fragments arranged by size:
3145 1578 1572
ApaI G_GGCC'C
Cuts at: 2441 2441
Size: 6295
ApaLI G'TGCA_C
Cuts at: 2852 4352 5508 2852
Size: 1500 1156 3639
Fragments arranged by size:
3639 1500 1156
ApoI r'AATT_y
Cuts at: 1493 3604 3830 3982 6091 1493
Size: 2111 226 152 2109 1697
Fragments arranged by size:
2111 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2487 3125 3620 1870
Size: 73 119 425 638 495 4545
Fragments arranged by size:
4545 638 495 425 119 73
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2525 3245 3616 5071 618
Size: 325 217 627 738 720 371 1455 1842
Fragments arranged by size:
1842 1455 738 720 627 371 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2040 2441 3028 3434 3614 1391
Size: 621 28 401 587 406 180 4072
Fragments arranged by size:
4072 621 587 406 401 180 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3274 928
Size: 971 1375 3949
Fragments arranged by size:
3949 1375 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2653 2687 2755 2789 4486 4520 1903
Size: 34 716 34 68 34 1697 34 3678
Fragments arranged by size:
3678 1697 716 68 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2161 4187 5397 2161
Size: 2026 1210 3059
Fragments arranged by size:
3059 2026 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2814 4906 136
Size: 107 122 71 631 605 1142 2092 1525
Fragments arranged by size:
2092 1525 1142 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 2193 2441 2528 2856 3248 3405 4356 5512
Size: 403 248 87 328 392 157 951 1156
Cuts at: 5512 1790
Size: 2573
Fragments arranged by size:
2573 1156 951 403 392 328 248 157
87
BmgBI CAC'GTC
Cuts at: 1318 2278 2467 1318
Size: 960 189 5146
Fragments arranged by size:
5146 960 189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4026 4984 442
Size: 416 53 83 399 24 2609 958 1753
Fragments arranged by size:
2609 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2747 4955 1239
Size: 884 624 2208 2579
Fragments arranged by size:
2579 2208 884 624
Bpu10I CC'TnA_GC
Cuts at: 2124 2124
Size: 6295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 4420 5285 5583 2051
Size: 2369 865 298 2763
Fragments arranged by size:
2763 2369 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3046 4086 493
Size: 711 1842 1040 2702
Fragments arranged by size:
2702 1842 1040 711
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 2877 4102 4484
Size: 53 83 186 936 618 726 1225 382
Cuts at: 4484 5119 275
Size: 635 1451
Fragments arranged by size:
1451 1225 936 726 635 618 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2720 2750 3062 3092 1178
Size: 30 165 30 1317 30 312 30 4381
Fragments arranged by size:
4381 1317 312 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2120 1186
Size: 862 72 5361
Fragments arranged by size:
5361 862 72
BseYI C'CCAG_C
Cuts at: 2039 2155 2893 2935 3314 3493 5498 5994 2039
Size: 116 738 42 379 179 2005 496 2340
Fragments arranged by size:
2340 2005 738 496 379 179 116 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 2527 2570 2732 3020 3499 3539
Size: 573 648 143 43 162 288 479 40
Cuts at: 3539 1163
Size: 3919
Fragments arranged by size:
3919 648 573 479 288 162 143 43
40
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2798 3745 4506 5534 1540
Size: 275 186 797 947 761 1028 2301
Fragments arranged by size:
2301 1028 947 797 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2627 2781 2856 2892 4356 4441 5512 2627
Size: 154 75 36 1464 85 1071 3410
Fragments arranged by size:
3410 1464 1071 154 85 75 36
BsmI GAATG_Cn'
Cuts at: 3806 3899 3806
Size: 93 6202
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2040 2193 2441 2528 2627 2781
Size: 399 222 28 153 248 87 99 154
Cuts at: 2781 2856 2892 3028 3248 3405 3434 3614 4356
Size: 75 36 136 220 157 29 180 742
Cuts at: 4356 4441 5512 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 248 222 220 180
157 154 153 136 99 87 85 75
36 29 28
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 3261 3650 844
Size: 1451 966 389 3489
Fragments arranged by size:
3489 1451 966 389
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4790 4964 65
Size: 1183 3542 174 1396
Fragments arranged by size:
3542 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2951 4939 5126 1700
Size: 110 1141 1988 187 2869
Fragments arranged by size:
2869 1988 1141 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6295
BssHII G'CGCG_C
Cuts at: 1925 2686 1925
Size: 761 5534
BssSI C'ACGA_G
Cuts at: 2942 4355 5367 2942
Size: 1413 1012 3870
Fragments arranged by size:
3870 1413 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6295
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 2947 1184
Size: 1763 4532
BstXI CCAn_nnnn'nTGG
Cuts at: 2512 3412 2512
Size: 900 5395
BstYI r'GATC_y
Cuts at: 1972 3135 3213 3625 3992 4377 4394 5846 5932
Size: 1163 78 412 367 385 17 1452 86
Cuts at: 5932 5944 6290 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1163 412 385 367 346 86
78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2138 2138
Size: 6295
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2529 2987 3231 3504 513
Size: 552 939 9 516 458 244 273 3304
Fragments arranged by size:
3304 939 552 516 458 273 244 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2237 3067 487
Size: 683 56 1011 830 3715
Fragments arranged by size:
3715 1011 830 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3362 3811 4605 4625 3142
Size: 220 449 794 20 4812
Fragments arranged by size:
4812 794 449 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DraIII CAC_nnn'GTG
Cuts at: 2947 3108 2947
Size: 161 6134
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3496 5302 809
Size: 507 2180 1806 1802
Fragments arranged by size:
2180 1806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2514 2795 2990
Size: 54 1425 50 275 18 684 281 195
Cuts at: 2990 3742 4631 6260 8
Size: 752 889 1629 43
Fragments arranged by size:
1629 1425 889 752 684 281 275 195
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2795 3742 1537
Size: 275 983 947 4090
Fragments arranged by size:
4090 983 947 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2486 2786 3299 4225 1353
Size: 1133 300 513 926 3423
Fragments arranged by size:
3423 1133 926 513 300
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2129 3128 3490 4882 5256 5402
Size: 1064 138 540 999 362 1392 374 146
Cuts at: 5402 387
Size: 1280
Fragments arranged by size:
1392 1280 1064 999 540 374 362 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2747 3004 4358 4955 5742 6010
Size: 393 491 624 257 1354 597 787 268
Cuts at: 6010 1239
Size: 1524
Fragments arranged by size:
1524 1354 787 624 597 491 393 268
257
EcoO109I rG'GnC_Cy
Cuts at: 2134 2307 2438 2964 2134
Size: 173 131 526 5465
Fragments arranged by size:
5465 526 173 131
EcoRI G'AATT_C
Cuts at: 3604 3982 3604
Size: 378 5917
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 2029 2726 5442 1678
Size: 351 697 2716 2531
Fragments arranged by size:
2716 2531 697 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2629 2661 3062 3094 4936
Size: 32 135 32 1057 32 401 32 1842
Cuts at: 4936 4968 5010 5042 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1842 1057 401 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5409 5593 1305
Size: 4104 184 2007
Fragments arranged by size:
4104 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4473
Fragments arranged by size:
4473 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 2345 2579 3086 3182 3467 4253
Size: 648 1110 69 234 507 96 285 786
Cuts at: 4253 4612 4771 518
Size: 359 159 2042
Fragments arranged by size:
2042 1110 786 648 507 359 285 234
159 96 69
MspA1I CmG'CkG
Cuts at: 1606 2101 2360 2519 2958 2989 3009 3318 3442
Size: 495 259 159 439 31 20 309 124
Cuts at: 3442 3549 3711 4388 5536 5781 1606
Size: 107 162 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 495 439 309 259 245
162 159 124 107 31 20
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4804
Fragments arranged by size:
4804 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 2974 3076 3325 3481 3653 5198 1132
Size: 97 1745 102 249 156 172 1545 2229
Fragments arranged by size:
2229 1745 1545 249 172 156 102 97
PasI CC'CwG_GG
Cuts at: 1348 2303 1348
Size: 955 5340
PciI A'CATG_T
Cuts at: 3477 5194 3477
Size: 1717 4578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3256 1108
Size: 2148 4147
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2307 2307
Size: 6295
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PspOMI G'GGCC_C
Cuts at: 2437 2437
Size: 6295
PspXI vC'TCGA_Gb
Cuts at: 3125 3125
Size: 6295
PstI C_TGCA'G
Cuts at: 830 2478 3647 830
Size: 1648 1169 3478
Fragments arranged by size:
3478 1648 1169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 2101 2360 3009 3549 2101
Size: 259 649 540 4847
Fragments arranged by size:
4847 649 540 259
SacII CC_GC'GG
Cuts at: 2990 2990
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SapI GCTCTTCn'nnn_
Cuts at: 2486 2786 2486
Size: 300 5995
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SfcI C'TryA_G
Cuts at: 826 1045 2474 3520 3643 4778 5459 5650 826
Size: 219 1429 1046 123 1135 681 191 1471
Fragments arranged by size:
1471 1429 1135 1046 681 219 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 2064 3622 1872
Size: 192 1558 4545
Fragments arranged by size:
4545 1558 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 3125 4399 5300 5562 820
Size: 197 926 87 1095 1274 901 262 1553
Fragments arranged by size:
1553 1274 1095 926 901 262 197 87
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3325 3653 1132
Size: 2193 328 3774
Fragments arranged by size:
3774 2193 328
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 2498 2826 3591 1517
Size: 981 328 765 4221
Fragments arranged by size:
4221 981 765 328
StyI C'CwwG_G
Cuts at: 513 1065 2004 2433 2993 3734 513
Size: 552 939 429 560 741 3074
Fragments arranged by size:
3074 939 741 560 552 429
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2907 3263 4322 4339 4492 4677 5122
Size: 1255 1327 356 1059 17 153 185 445
Cuts at: 5122 325
Size: 1498
Fragments arranged by size:
1498 1327 1255 1059 445 356 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4541 6253 96
Size: 274 80 33 51 1443 2564 1712 138
Fragments arranged by size:
2564 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2720 2752 6255 6287 2720
Size: 32 3503 32 2728
Fragments arranged by size:
3503 2728 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2864 3469 1316
Size: 1548 605 4142
Fragments arranged by size:
4142 1548 605
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3175 2011
Size: 1164 5131
XhoI C'TCGA_G
Cuts at: 1943 3125 1943
Size: 1182 5113
XmaI C'CCGG_G
Cuts at: 1870 2062 3620 1870
Size: 192 1558 4545
Fragments arranged by size:
4545 1558 192
XmnI GAAnn'nnTTC
Cuts at: 4424 4424
Size: 6295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2878 4103 276
Size: 53 83 186 936 1344 1225 2468
Fragments arranged by size:
2468 1344 1225 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PsrI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI