Restriction map for
pFN21AE2609
(Circular) (Six-base) MAPSORT of: pFN21AE2609.seq Check: 5519 from: 1 to: 5500
pFN21AE2609 5500 bp
With 182 enzymes: SgfI *
February 12, 2016 18:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3310 278
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2843 1937
Size: 906 4594
Acc65I G'GTAC_C
Cuts at: 2821 2821
Size: 5500
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2001 3563 4947 5215 1632
Size: 369 1562 1384 268 1917
Fragments arranged by size:
1917 1562 1384 369 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5303
AflIII A'CryG_T
Cuts at: 1203 2374 4399 5376 1203
Size: 1171 2025 977 1327
Fragments arranged by size:
2025 1327 1171 977
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 4229 4229
Size: 5500
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5500
AlwNI CAG_nnn'CTG
Cuts at: 3243 4815 3243
Size: 1572 3928
ApaI G_GGCC'C
Cuts at: 2286 2286
Size: 5500
ApaLI G'TGCA_C
Cuts at: 3557 4713 3557
Size: 1156 4344
ApoI r'AATT_y
Cuts at: 1493 2809 3035 3187 5296 1493
Size: 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4054 160
Size: 872 3022 1606
Fragments arranged by size:
3022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2825 1870
Size: 73 882 4545
Fragments arranged by size:
4545 882 73
BamHI G'GATC_C
Cuts at: 2830 3197 2830
Size: 367 5133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2672 2821 4276 618
Size: 325 217 627 885 149 1455 1842
Fragments arranged by size:
1842 1455 885 627 325 217 149
BanII G_rGCy'C
Cuts at: 1391 2058 2286 2819 1391
Size: 667 228 533 4072
Fragments arranged by size:
4072 667 533 228
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3691 3725 1903
Size: 34 1754 34 3678
Fragments arranged by size:
3678 1754 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 4602 3392
Size: 1210 4290
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4111 136
Size: 107 122 71 631 605 2439 1525
Fragments arranged by size:
2439 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5495 5495
Size: 5500
BlpI GC'TnA_GC
Cuts at: 2917 2917
Size: 5500
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2286 3561 4717 1790
Size: 317 179 1275 1156 2573
Fragments arranged by size:
2573 1275 1156 317 179
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2660 2684 3231
Size: 416 53 83 399 24 1243 24 547
Cuts at: 3231 4189 442
Size: 958 1753
Fragments arranged by size:
1753 1243 958 547 416 399 83 53
24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4160 1239
Size: 2921 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4490 4788 3625
Size: 865 298 4337
Fragments arranged by size:
4337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4163 882
Size: 617 2664 2219
Fragments arranged by size:
2664 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2425 3291 493
Size: 711 1221 866 2702
Fragments arranged by size:
2702 1221 866 711
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3307 3689 4324 275
Size: 53 83 186 936 1774 382 635 1451
Fragments arranged by size:
1774 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2473 3933 4331 4605 4752 5163 2473
Size: 1460 398 274 147 411 2810
Fragments arranged by size:
2810 1460 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5275
Fragments arranged by size:
5275 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5500
BseYI C'CCAG_C
Cuts at: 2747 2783 4703 5199 2747
Size: 36 1920 496 3048
Fragments arranged by size:
3048 1920 496 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4927
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2299 2950 3711 4739 1540
Size: 275 186 298 651 761 1028 2301
Fragments arranged by size:
2301 1028 761 651 298 275 186
BsiHKAI G_wGCw'C
Cuts at: 3561 3646 4717 3561
Size: 85 1071 4344
Fragments arranged by size:
4344 1071 85
BsmI GAATG_Cn'
Cuts at: 2040 3011 3104 2040
Size: 971 93 4436
Fragments arranged by size:
4436 971 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2107 2286 2819 3561 3646 4717
Size: 399 268 49 179 533 742 85 1071
Cuts at: 4717 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 533 399 268 179 85
49
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 2766 3389 5119 2766
Size: 623 1730 3147
Fragments arranged by size:
3147 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2100 2855 844
Size: 1256 755 3489
Fragments arranged by size:
3489 1256 755
BsrBI CCG'CTC
Cuts at: 1394 1823 3387 1394
Size: 429 1564 3507
Fragments arranged by size:
3507 1564 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3995 4169 65
Size: 1183 2747 174 1396
Fragments arranged by size:
2747 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4144 4331 1700
Size: 110 2334 187 2869
Fragments arranged by size:
2869 2334 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5500
BssSI C'ACGA_G
Cuts at: 3560 4572 3560
Size: 1012 4488
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5500
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5500
BstYI r'GATC_y
Cuts at: 1972 2830 3197 3582 3599 5051 5137 5149 5495
Size: 858 367 385 17 1452 86 12 346
Cuts at: 5495 1972
Size: 1977
Fragments arranged by size:
1977 1452 858 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4009
Fragments arranged by size:
4009 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4761
Fragments arranged by size:
4761 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3016 3810 3830 2689
Size: 327 794 20 4359
Fragments arranged by size:
4359 794 327 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5465
DraI TTT'AAA
Cuts at: 2804 2804
Size: 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4507 809
Size: 507 3191 1802
Fragments arranged by size:
3191 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2947 3836 5465
Size: 54 1425 50 275 18 1117 889 1629
Cuts at: 5465 8
Size: 43
Fragments arranged by size:
1629 1425 1117 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2947 1537
Size: 275 1135 4090
Fragments arranged by size:
4090 1135 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3430 1353
Size: 2077 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4087 4461 4607 387
Size: 1064 138 2498 374 146 1280
Fragments arranged by size:
2498 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2001 3563 4160 4947 5215 1239
Size: 393 369 1562 597 787 268 1524
Fragments arranged by size:
1562 1524 787 597 393 369 268
EcoO109I rG'GnC_Cy
Cuts at: 2155 2282 2601 2639 2155
Size: 127 319 38 5016
Fragments arranged by size:
5016 319 127 38
EcoRI G'AATT_C
Cuts at: 2809 3187 2809
Size: 378 5122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2408 2408
Size: 5500
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 2622 4647 1678
Size: 944 2025 2531
Fragments arranged by size:
2531 2025 944
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4141 4173 4215 4247 1373
Size: 32 135 32 2569 32 42 32 2626
Fragments arranged by size:
2626 2569 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2510 2844 3090 669
Size: 988 281 572 334 246 3079
Fragments arranged by size:
3079 988 572 334 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5500
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KpnI G_GTAC'C
Cuts at: 2825 2825
Size: 5500
MfeI C'AATT_G
Cuts at: 3099 3099
Size: 5500
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4614 4798 1305
Size: 3309 184 2007
Fragments arranged by size:
3309 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3678
Fragments arranged by size:
3678 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3458 3817 3976 518
Size: 648 2292 359 159 2042
Fragments arranged by size:
2292 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2747 2756 2916 3593 4741 4986 1606
Size: 1141 9 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 1141 677 245 160 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4009
Fragments arranged by size:
4009 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5500
NotI GC'GGCC_GC
Cuts at: 2947 2947
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2228 2858 4403 1132
Size: 97 999 630 1545 2229
Fragments arranged by size:
2229 1545 999 630 97
PasI CC'CwG_GG
Cuts at: 1348 2279 1348
Size: 931 4569
PciI A'CATG_T
Cuts at: 4399 4399
Size: 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5500
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 5082 5114 3569
Size: 32 1481 32 3955
Fragments arranged by size:
3955 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2601 2639 2155
Size: 446 38 5016
Fragments arranged by size:
5016 446 38
PshAI GACnn'nnGTC
Cuts at: 1613 2756 1613
Size: 1143 4357
PsiI TTA'TAA
Cuts at: 2381 3070 2381
Size: 689 4811
PspOMI G'GGCC_C
Cuts at: 2282 2282
Size: 5500
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2516 2548 2516
Size: 32 5468
PstI C_TGCA'G
Cuts at: 830 2852 830
Size: 2022 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5500
PvuII CAG'CTG
Cuts at: 2747 2747
Size: 5500
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SbfI CC_TGCA'GG
Cuts at: 2852 2852
Size: 5500
ScaI AGT'ACT
Cuts at: 2778 3748 5460 2778
Size: 970 1712 2818
Fragments arranged by size:
2818 1712 970
SfcI C'TryA_G
Cuts at: 826 1045 2848 3983 4664 4855 826
Size: 219 1803 1135 681 191 1471
Fragments arranged by size:
1803 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2827 1872
Size: 955 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3604 4505 4767 820
Size: 197 926 1661 901 262 1553
Fragments arranged by size:
1661 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2425 3291 493
Size: 1932 866 2702
Fragments arranged by size:
2702 1932 866
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2228 2858 1132
Size: 1096 630 3774
Fragments arranged by size:
3774 1096 630
SspI AAT'ATT
Cuts at: 5 52 2116 3424 5
Size: 47 2064 1308 2081
Fragments arranged by size:
2081 2064 1308 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5500
StyI C'CwwG_G
Cuts at: 513 1065 2004 2939 513
Size: 552 939 935 3074
Fragments arranged by size:
3074 939 935 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2360 2455 2660 3527 3544 3697 3882
Size: 1255 780 95 205 867 17 153 185
Cuts at: 3882 4327 325
Size: 445 1498
Fragments arranged by size:
1498 1255 867 780 445 205 185 153
95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2719 2776 3746
Size: 274 80 33 51 1443 742 57 970
Cuts at: 3746 5458 96
Size: 1712 138
Fragments arranged by size:
1712 1443 970 742 274 138 80 57
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 5468
Tth111I GACn'n_nGTC
Cuts at: 1316 2690 1316
Size: 1374 4126
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2825 1870
Size: 955 4545
XmnI GAAnn'nnTTC
Cuts at: 3629 3629
Size: 5500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3308 276
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI