Restriction map for
pFN21AE2607
(Circular) (Six-base) MAPSORT of: pFN21AE2607.seq Check: 7611 from: 1 to: 8017
pFN21AE2607 8017 bp
With 182 enzymes: SgfI *
September 11, 2017 14:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5827 278
Size: 53 83 186 936 4291 2468
Fragments arranged by size:
4291 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4155 5360 1937
Size: 2218 1205 4594
Fragments arranged by size:
4594 2218 1205
Acc65I G'GTAC_C
Cuts at: 4766 5338 4766
Size: 572 7445
AclI AA'CG_TT
Cuts at: 1563 6144 6517 1563
Size: 4581 373 3063
Fragments arranged by size:
4581 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2482 2495 3235 3314 3490 3698 3818 6080
Size: 850 13 740 79 176 208 120 2262
Cuts at: 6080 7464 7732 1632
Size: 1384 268 1917
Fragments arranged by size:
2262 1917 1384 850 740 268 208 176
120 79 13
AfeI AGC'GCT
Cuts at: 4688 4688
Size: 8017
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7820
AflIII A'CryG_T
Cuts at: 1203 3660 4893 6916 7893 1203
Size: 2457 1233 2023 977 1327
Fragments arranged by size:
2457 2023 1327 1233 977
AgeI A'CCGG_T
Cuts at: 6848 6848
Size: 8017
AhdI GACnn_n'nnGTC
Cuts at: 6746 6746
Size: 8017
AleI CACnn'nnGTG
Cuts at: 1166 3515 1166
Size: 2349 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8017
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4690 4722 4690
Size: 32 7985
AlwNI CAG_nnn'CTG
Cuts at: 3762 3934 5760 7332 3762
Size: 172 1826 1572 4447
Fragments arranged by size:
4447 1826 1572 172
ApaLI G'TGCA_C
Cuts at: 6074 7230 6074
Size: 1156 6861
ApoI r'AATT_y
Cuts at: 1493 2131 2146 2294 2973 3153 3544 4030 5326
Size: 638 15 148 679 180 391 486 1296
Cuts at: 5326 5552 5704 7813 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1296 679 638 486 391 226
180 152 148 15
AseI AT'TA_AT
Cuts at: 160 1032 6571 160
Size: 872 5539 1606
Fragments arranged by size:
5539 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8017
AvaI C'yCGr_G
Cuts at: 1870 1943 2998 3873 4065 4304 4859 5342 1870
Size: 73 1055 875 192 239 555 483 4545
Fragments arranged by size:
4545 1055 875 555 483 239 192 73
BamHI G'GATC_C
Cuts at: 4665 5347 5714 4665
Size: 682 367 6968
Fragments arranged by size:
6968 682 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2243 2415 2456 2552 3678
Size: 325 217 627 456 172 41 96 1126
Cuts at: 3678 4291 4747 4766 4955 5338 6793 618
Size: 613 456 19 189 383 1455 1842
Fragments arranged by size:
1842 1455 1126 627 613 456 456 383
325 217 189 172 96 41 19
BanII G_rGCy'C
Cuts at: 1391 4725 5336 1391
Size: 3334 611 4072
Fragments arranged by size:
4072 3334 611
BbeI G_GCGC'C
Cuts at: 2460 2460
Size: 8017
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2161 3624 3806 3921 4207 4225 5047
Size: 971 262 1463 182 115 286 18 822
Cuts at: 5047 5115 5154 5279 928
Size: 68 39 125 3666
Fragments arranged by size:
3666 1463 971 822 286 262 182 125
115 68 39 18
BbvCI CC'TCA_GC
Cuts at: 2700 2700
Size: 8017
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3812 3846 6208 6242 1903
Size: 34 1875 34 2362 34 3678
Fragments arranged by size:
3678 2362 1875 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5254 5909 7119 5254
Size: 655 1210 6152
Fragments arranged by size:
6152 1210 655
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8017
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6628 136
Size: 107 122 71 631 605 4956 1525
Fragments arranged by size:
4956 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2493 8012 2493
Size: 5519 2498
BlpI GC'TnA_GC
Cuts at: 2027 5434 2027
Size: 3407 4610
Bme1580I G_kGCm'C
Cuts at: 1790 4752 4958 6078 7234 1790
Size: 2962 206 1120 1156 2573
Fragments arranged by size:
2962 2573 1156 1120 206
BmgBI CAC'GTC
Cuts at: 1318 3661 4759 1318
Size: 2343 1098 4576
Fragments arranged by size:
4576 2343 1098
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2345 2713 4746
Size: 416 53 83 399 24 928 368 2033
Cuts at: 4746 4809 5748 6706 442
Size: 63 939 958 1753
Fragments arranged by size:
2033 1753 958 939 928 416 399 368
83 63 53 24
BmtI G_CTAG'C
Cuts at: 1056 3027 1056
Size: 1971 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 3326 3458 3995 4711 6677 1239
Size: 826 1261 132 537 716 1966 2579
Fragments arranged by size:
2579 1966 1261 826 716 537 132
Bpu10I CC'TnA_GC
Cuts at: 2700 2700
Size: 8017
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5264 6142 7007 7305 5264
Size: 878 865 298 5976
Fragments arranged by size:
5976 878 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2668 3863 6680 882
Size: 617 1169 1195 2817 2219
Fragments arranged by size:
2817 2219 1195 1169 617
BsaAI yAC'GTr
Cuts at: 493 1204 4267 4366 4773 5808 493
Size: 711 3063 99 407 1035 2702
Fragments arranged by size:
3063 2702 1035 711 407 99
BsaBI GATnn'nnATC
Cuts at: 4595 4871 8011 4595
Size: 276 3140 4601
Fragments arranged by size:
4601 3140 276
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2307 2457 5824 6206
Size: 53 83 186 936 774 150 3367 382
Cuts at: 6206 6841 275
Size: 635 1451
Fragments arranged by size:
3367 1451 936 774 635 382 186 150
83 53
BsaWI w'CCGG_w
Cuts at: 2835 3522 3609 6450 6848 7122 7269 7680 2835
Size: 687 87 2841 398 274 147 411 3172
Fragments arranged by size:
3172 2841 687 411 398 274 147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 1178
Size: 30 165 30 786 30 6976
Fragments arranged by size:
6976 786 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 3131 3134 3554 3863 1186
Size: 1450 495 3 420 309 5340
Fragments arranged by size:
5340 1450 495 420 309 3
BseYI C'CCAG_C
Cuts at: 2406 3200 3836 4014 4414 7220 7716 2406
Size: 794 636 178 400 2806 496 2707
Fragments arranged by size:
2806 2707 794 636 496 400 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3748 4774 5030 1163
Size: 573 2012 1026 256 4150
Fragments arranged by size:
4150 2012 1026 573 256
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5467 6228 7256 1540
Size: 275 186 3466 761 1028 2301
Fragments arranged by size:
3466 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2099 4842 6078 6163 7234 2099
Size: 2743 1236 85 1071 2882
Fragments arranged by size:
2882 2743 1236 1071 85
BsmI GAATG_Cn'
Cuts at: 5528 5621 5528
Size: 93 7924
BsmBI CGTCTCn'nnnn_
Cuts at: 2094 2094
Size: 8017
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 4725 4752 4842 4958 5336 6078
Size: 399 309 2626 27 90 116 378 742
Cuts at: 6078 6163 7234 1391
Size: 85 1071 2174
Fragments arranged by size:
2626 2174 1071 742 399 378 309 116
90 85 27
BspEI T'CCGG_A
Cuts at: 2835 3609 7680 2835
Size: 774 4071 3172
Fragments arranged by size:
4071 3172 774
BspHI T'CATG_A
Cuts at: 5178 5906 7636 5178
Size: 728 1730 5559
Fragments arranged by size:
5559 1730 728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4854 4932 5372 844
Size: 4010 78 440 3489
Fragments arranged by size:
4010 3489 440 78
BsrBI CCG'CTC
Cuts at: 1394 1823 5904 1394
Size: 429 4081 3507
Fragments arranged by size:
4081 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3226 4052 6512 6686 65
Size: 1183 1978 826 2460 174 1396
Fragments arranged by size:
2460 1978 1396 1183 826 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2502 6661 6848 1700
Size: 110 692 4159 187 2869
Fragments arranged by size:
4159 2869 692 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8017
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8017
BssSI C'ACGA_G
Cuts at: 2789 6077 7089 2789
Size: 3288 1012 3717
Fragments arranged by size:
3717 3288 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3685 4591 4846 794
Size: 2891 906 255 3965
Fragments arranged by size:
3965 2891 906 255
BstBI TT'CG_AA
Cuts at: 6821 6821
Size: 8017
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8017
BstXI CCAn_nnnn'nTGG
Cuts at: 2927 4759 4879 2927
Size: 1832 120 6065
Fragments arranged by size:
6065 1832 120
BstYI r'GATC_y
Cuts at: 1972 2493 2746 2898 4665 5347 5714 6099 6116
Size: 521 253 152 1767 682 367 385 17
Cuts at: 6116 7568 7654 7666 8012 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1767 1452 682 521 385 367 346
253 152 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3114 4670 3114
Size: 1556 6461
BtgI C'CryG_G
Cuts at: 513 1065 2193 513
Size: 552 1128 6337
Fragments arranged by size:
6337 1128 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2500 2709 3924 3994 4225 487
Size: 683 56 1274 209 1215 70 231 4279
Fragments arranged by size:
4279 1274 1215 683 231 209 70 56
BtsI GCAGTG_nn'
Cuts at: 4786 5533 6327 6347 4786
Size: 747 794 20 6456
Fragments arranged by size:
6456 794 747 20
ClaI AT'CG_AT
Cuts at: 1554 4599 5245 1554
Size: 3045 646 4326
Fragments arranged by size:
4326 3045 646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2872 2907 529
Size: 35 2308 35 5639
Fragments arranged by size:
5639 2308 35 35
DraI TTT'AAA
Cuts at: 5321 5321
Size: 8017
DraIII CAC_nnn'GTG
Cuts at: 3661 3897 4823 3661
Size: 236 926 6855
Fragments arranged by size:
6855 926 236
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7024 809
Size: 507 5708 1802
Fragments arranged by size:
5708 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2634 3628 3807
Size: 54 1425 50 275 18 804 994 179
Cuts at: 3807 4653 4731 4741 5464 6353 7982 8
Size: 846 78 10 723 889 1629 43
Fragments arranged by size:
1629 1425 994 889 846 804 723 275
179 78 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 5464 1537
Size: 275 3652 4090
Fragments arranged by size:
4090 3652 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3497 3595 3701 4343 4465 5947 1353
Size: 2144 98 106 642 122 1482 3423
Fragments arranged by size:
3423 2144 1482 642 122 106 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3416 6604 6978 7124 387
Size: 1064 138 1827 3188 374 146 1280
Fragments arranged by size:
3188 1827 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2482 2495 3235 3314 3326 3458
Size: 393 433 417 13 740 79 12 132
Cuts at: 3458 3490 3698 3818 3995 4711 6080 6677 7464
Size: 32 208 120 177 716 1369 597 787
Cuts at: 7464 7732 1239
Size: 268 1524
Fragments arranged by size:
1524 1369 787 740 716 597 433 417
393 268 208 177 132 120 79 32
13 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3032 5009 3032
Size: 1977 6040
EcoO109I rG'GnC_Cy
Cuts at: 4565 4565
Size: 8017
EcoRI G'AATT_C
Cuts at: 3544 5326 5704 3544
Size: 1782 378 5857
Fragments arranged by size:
5857 1782 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4685 5104 4685
Size: 419 7598
FspI TGC'GCA
Cuts at: 2489 6523 2489
Size: 4034 3983
HaeII r_GCGC'y
Cuts at: 1678 2460 4690 7164 1678
Size: 782 2230 2474 2531
Fragments arranged by size:
2531 2474 2230 782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2151 2183 6658 6690 6732
Size: 32 135 32 579 32 4475 32 42
Cuts at: 6732 6764 1373
Size: 32 2626
Fragments arranged by size:
4475 2626 579 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4156 4921 5361 5607 669
Size: 988 281 2218 765 440 246 3079
Fragments arranged by size:
3079 2218 988 765 440 281 246
HindIII A'AGCT_T
Cuts at: 748 3296 3791 3951 5267 748
Size: 2548 495 160 1316 3498
Fragments arranged by size:
3498 2548 1316 495 160
HpaI GTT'AAC
Cuts at: 5607 5607
Size: 8017
KasI G'GCGC_C
Cuts at: 2456 2456
Size: 8017
KpnI G_GTAC'C
Cuts at: 4770 5342 4770
Size: 572 7445
MfeI C'AATT_G
Cuts at: 5616 5616
Size: 8017
MluI A'CGCG_T
Cuts at: 7893 7893
Size: 8017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 2907 3113 3522 7131 7315 1305
Size: 1386 216 206 409 3609 184 2007
Fragments arranged by size:
3609 2007 1386 409 216 206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2636 3809 4655 4733 10
Size: 54 1425 343 804 1173 846 78 3294
Fragments arranged by size:
3294 1425 1173 846 804 343 78 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 3515 4892 5075 5975 6334 6493
Size: 648 1278 1071 1377 183 900 359 159
Cuts at: 6493 518
Size: 2042
Fragments arranged by size:
2042 1377 1278 1071 900 648 359 183
159
MspA1I CmG'CkG
Cuts at: 1606 3292 3319 3931 5433 6110 7258 7503 1606
Size: 1686 27 612 1502 677 1148 245 2120
Fragments arranged by size:
2120 1686 1502 1148 677 612 245 27
NaeI GCC'GGC
Cuts at: 2504 2504
Size: 8017
NarI GG'CG_CC
Cuts at: 2457 2457
Size: 8017
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7465
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8017
NgoMIV G'CCGG_C
Cuts at: 2502 2502
Size: 8017
NheI G'CTAG_C
Cuts at: 1052 3023 1052
Size: 1971 6046
NotI GC'GGCC_GC
Cuts at: 5464 5464
Size: 8017
NspI r_CATG'y
Cuts at: 1132 1229 2488 4897 5375 6920 1132
Size: 97 1259 2409 478 1545 2229
Fragments arranged by size:
2409 2229 1545 1259 478 97
PasI CC'CwG_GG
Cuts at: 1348 3778 1348
Size: 2430 5587
PciI A'CATG_T
Cuts at: 4893 6916 4893
Size: 2023 5994
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 4080 1108
Size: 1128 1844 5045
Fragments arranged by size:
5045 1844 1128
PfoI T'CCnGG_A
Cuts at: 2760 4220 4962 2760
Size: 1460 742 5815
Fragments arranged by size:
5815 1460 742
PmeI GTTT'AAAC
Cuts at: 5321 5321
Size: 8017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6086 6118 7599 7631 6086
Size: 32 1481 32 6472
Fragments arranged by size:
6472 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4565 4565
Size: 8017
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8017
PsiI TTA'TAA
Cuts at: 4577 5587 4577
Size: 1010 7007
PstI C_TGCA'G
Cuts at: 830 3534 4587 5024 5369 830
Size: 2704 1053 437 345 3478
Fragments arranged by size:
3478 2704 1053 437 345
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8017
PvuII CAG'CTG
Cuts at: 3292 3319 3931 3292
Size: 27 612 7378
Fragments arranged by size:
7378 612 27
SalI G'TCGA_C
Cuts at: 1936 4154 5359 1936
Size: 2218 1205 4594
Fragments arranged by size:
4594 2218 1205
SbfI CC_TGCA'GG
Cuts at: 5369 5369
Size: 8017
ScaI AGT'ACT
Cuts at: 5018 6265 7977 5018
Size: 1247 1712 5058
Fragments arranged by size:
5058 1712 1247
SexAI A'CCwGG_T
Cuts at: 2433 4977 2433
Size: 2544 5473
SfcI C'TryA_G
Cuts at: 826 1045 3530 4583 5020 5365 6500 7181 7372
Size: 219 2485 1053 437 345 1135 681 191
Cuts at: 7372 826
Size: 1471
Fragments arranged by size:
2485 1471 1135 1053 681 437 345 219
191
SfoI GGC'GCC
Cuts at: 2458 2458
Size: 8017
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8017
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8017
SmaI CCC'GGG
Cuts at: 1872 3875 5344 1872
Size: 2003 1469 4545
Fragments arranged by size:
4545 2003 1469
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5279 6121 7022 7284 820
Size: 197 926 3336 842 901 262 1553
Fragments arranged by size:
3336 1553 926 901 842 262 197
SnaBI TAC'GTA
Cuts at: 493 4366 5808 493
Size: 3873 1442 2702
Fragments arranged by size:
3873 2702 1442
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8017
SphI G_CATG'C
Cuts at: 1132 2488 5375 1132
Size: 1356 2887 3774
Fragments arranged by size:
3774 2887 1356
SspI AAT'ATT
Cuts at: 5 52 2629 5941 5
Size: 47 2577 3312 2081
Fragments arranged by size:
3312 2577 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8017
StyI C'CwwG_G
Cuts at: 513 1065 2187 2289 2920 4113 5456 513
Size: 552 1122 102 631 1193 1343 3074
Fragments arranged by size:
3074 1343 1193 1122 631 552 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 2443 2570 3485 4699 6044 6061
Size: 1255 681 182 127 915 1214 1345 17
Cuts at: 6061 6214 6399 6844 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1345 1255 1214 915 681 445 185
182 153 127 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2076 2574 4317
Size: 274 80 33 51 1443 99 498 1743
Cuts at: 4317 5016 6263 7975 96
Size: 699 1247 1712 138
Fragments arranged by size:
1743 1712 1443 1247 699 498 274 138
99 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2189 2221 2731 2742 2763 2774 7977 8009 2189
Size: 32 510 11 21 11 5203 32 2197
Fragments arranged by size:
5203 2197 510 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 4979 1316
Size: 3663 4354
XbaI T'CTAG_A
Cuts at: 5353 5353
Size: 8017
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4912 4912
Size: 8017
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8017
XmaI C'CCGG_G
Cuts at: 1870 3873 5342 1870
Size: 2003 1469 4545
Fragments arranged by size:
4545 2003 1469
XmnI GAAnn'nnTTC
Cuts at: 5087 6146 5087
Size: 1059 6958
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5825 276
Size: 53 83 186 936 4291 2468
Fragments arranged by size:
4291 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SrfI SwaI