Restriction map for
pFN21AE2599
(Circular) (Six-base) MAPSORT of: pFN21AE2599.seq Check: 6328 from: 1 to: 5923
pFN21AE2599 5923 bp
With 182 enzymes: SgfI *
January 6, 2016 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2020 2020
Size: 5923
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 2397 3244 2397
Size: 847 5076
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2801 3075 3179 3986 5370 5638 1632
Size: 479 690 274 104 807 1384 268 1917
Fragments arranged by size:
1917 1384 807 690 479 274 268 104
AfeI AGC'GCT
Cuts at: 2909 2909
Size: 5923
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 2341 4822 5799 1203
Size: 1138 2481 977 1327
Fragments arranged by size:
2481 1327 1138 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 3101 4652 3101
Size: 1551 4372
AleI CACnn'nnGTG
Cuts at: 1166 2489 1166
Size: 1323 4600
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 3148 3666 5238 3148
Size: 518 1572 3833
Fragments arranged by size:
3833 1572 518
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 3232 3458 3610 5719 1493
Size: 1739 226 152 2109 1697
Fragments arranged by size:
2109 1739 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2176 3248 1870
Size: 73 233 1072 4545
Fragments arranged by size:
4545 1072 233 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2389 2422 2389
Size: 33 5890
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2397 2453 2858 3244
Size: 325 217 627 221 389 56 405 386
Cuts at: 3244 4699 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 405 389 386 325 221
217 56
BanII G_rGCy'C
Cuts at: 1391 2292 2667 3242 1391
Size: 901 375 575 4072
Fragments arranged by size:
4072 901 575 375
BbeI G_GCGC'C
Cuts at: 2457 2862 2457
Size: 405 5518
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2101 4534 136
Size: 107 122 71 631 605 429 2433 1525
Fragments arranged by size:
2433 1525 631 605 429 122 107 71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2486 2611 3984 5140 1790
Size: 696 125 1373 1156 2573
Fragments arranged by size:
2573 1373 1156 696 125
BmgBI CAC'GTC
Cuts at: 1318 2344 2791 1318
Size: 1026 447 4450
Fragments arranged by size:
4450 1026 447
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3654 4612 442
Size: 416 53 83 399 24 2237 958 1753
Fragments arranged by size:
2237 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2681 3008 3158 4583 1239
Size: 1442 327 150 1425 2579
Fragments arranged by size:
2579 1442 1425 327 150
Bpu10I CC'TnA_GC
Cuts at: 2949 3215 2949
Size: 266 5657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 2946 4048 4913 5211 2651
Size: 295 1102 865 298 3363
Fragments arranged by size:
3363 1102 865 298 295
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2454 2859 3174 3730
Size: 53 83 186 936 921 405 315 556
Cuts at: 3730 4112 4747 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 921 635 556 405 382 315
186 83 53
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2493 2523 1178
Size: 30 165 30 1090 30 4578
Fragments arranged by size:
4578 1090 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5923
BseYI C'CCAG_C
Cuts at: 2096 2264 2554 3075 3193 5126 5622 2096
Size: 168 290 521 118 1933 496 2397
Fragments arranged by size:
2397 1933 521 496 290 168 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5350
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2142 2146 3373 4134 5162 1540
Size: 275 186 141 4 1227 761 1028 2301
Fragments arranged by size:
2301 1227 1028 761 275 186 141 4
BsiHKAI G_wGCw'C
Cuts at: 2667 3984 4069 5140 2667
Size: 1317 85 1071 3450
Fragments arranged by size:
3450 1317 1071 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2486 2611 2667 3242 3984 4069
Size: 399 502 194 125 56 575 742 85
Cuts at: 4069 5140 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 575 502 399 194 125
85 56
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2020 3278 844
Size: 1176 1258 3489
Fragments arranged by size:
3489 1258 1176
BsrBI CCG'CTC
Cuts at: 1394 1823 3214 3810 1394
Size: 429 1391 596 3507
Fragments arranged by size:
3507 1391 596 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2544 4418 4592 65
Size: 1183 1296 1874 174 1396
Fragments arranged by size:
1874 1396 1296 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 3125 4567 4754 1700
Size: 110 290 1025 1442 187 2869
Fragments arranged by size:
2869 1442 1025 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2935 3253 3620 4005 4022 5474 5560 5572
Size: 963 318 367 385 17 1452 86 12
Cuts at: 5572 5918 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 963 385 367 346 318 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2894 2894
Size: 5923
BtgI C'CryG_G
Cuts at: 513 1065 2004 2478 2754 2954 513
Size: 552 939 474 276 200 3482
Fragments arranged by size:
3482 939 552 474 276 200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2455 2720 2835 3439 4233 4253 2455
Size: 265 115 604 794 20 4125
Fragments arranged by size:
4125 794 604 265 115 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 2976 3227 2976
Size: 251 5672
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2366 4930 809
Size: 507 1050 2564 1802
Fragments arranged by size:
2564 1802 1050 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2514 2592
Size: 54 1425 50 275 18 313 371 78
Cuts at: 2592 2832 3370 4259 5888 8
Size: 240 538 889 1629 43
Fragments arranged by size:
1629 1425 889 538 371 313 275 240
78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2143 3370 1537
Size: 275 331 1227 4090
Fragments arranged by size:
4090 1227 331 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3853 1353
Size: 2500 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2875 4510 4884 5030 387
Size: 1064 138 1286 1635 374 146 1280
Fragments arranged by size:
1635 1286 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2665 2665
Size: 5923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2681 2801 3008 3075 3158 3179
Size: 393 479 570 120 207 67 83 21
Cuts at: 3179 3986 4583 5370 5638 1239
Size: 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 570 479 393 268
207 120 83 67 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2316 2893 2316
Size: 577 5346
EcoO109I rG'GnC_Cy
Cuts at: 2309 2473 3004 3018 2309
Size: 164 531 14 5214
Fragments arranged by size:
5214 531 164 14
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2265 2457 2862 2911 5070 1678
Size: 587 192 405 49 2159 2531
Fragments arranged by size:
2531 2159 587 405 192 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2226 2258 3087 3119 4564
Size: 32 135 32 654 32 829 32 1445
Cuts at: 4564 4596 4638 4670 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1445 829 654 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2359 2968 3123 3267 3513 669
Size: 988 281 421 609 155 144 246 3079
Fragments arranged by size:
3079 988 609 421 281 246 155 144
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KasI G'GCGC_C
Cuts at: 2453 2858 2453
Size: 405 5518
KpnI G_GTAC'C
Cuts at: 2401 3248 2401
Size: 847 5076
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2594 10
Size: 54 1425 343 762 3339
Fragments arranged by size:
3339 1425 762 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 3881 4240 4399 518
Size: 648 1323 1392 359 159 2042
Fragments arranged by size:
2042 1392 1323 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 3145 3339 4016 5164 5409 1606
Size: 510 1029 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 1029 677 510 245 194
NaeI GCC'GGC
Cuts at: 2102 2102
Size: 5923
NarI GG'CG_CC
Cuts at: 2454 2859 2454
Size: 405 5518
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4432
Fragments arranged by size:
4432 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NgoMIV G'CCGG_C
Cuts at: 2100 2100
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3281 4826 1132
Size: 97 2052 1545 2229
Fragments arranged by size:
2229 2052 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5923
PfoI T'CCnGG_A
Cuts at: 3081 3081
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2473 2473
Size: 5923
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 3145 3145
Size: 5923
SacI G_AGCT'C
Cuts at: 2667 2667
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SanDI GG'GwC_CC
Cuts at: 2473 2473
Size: 5923
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 3271 4406 5087 5278 826
Size: 219 2226 1135 681 191 1471
Fragments arranged by size:
2226 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2455 2860 2455
Size: 405 5518
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 2178 3250 1872
Size: 306 1072 4545
Fragments arranged by size:
4545 1072 306
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 2961 4027 4928 5190 820
Size: 197 926 723 295 1066 901 262 1553
Fragments arranged by size:
1553 1066 926 901 723 295 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 3362 513
Size: 552 939 1358 3074
Fragments arranged by size:
3074 1358 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2128 2374 3950 3967 4120 4305 4750
Size: 1255 548 246 1576 17 153 185 445
Cuts at: 4750 325
Size: 1498
Fragments arranged by size:
1576 1498 1255 548 445 246 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2541 4169 5881
Size: 274 80 33 51 1443 564 1628 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1712 1628 1443 564 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2408 2752 2408
Size: 344 5579
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 2176 3248 1870
Size: 306 1072 4545
Fragments arranged by size:
4545 1072 306
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SapI SexAI SfiI SrfI SwaI