Restriction map for
pFN21AE2598
(Circular) (Six-base) MAPSORT of: pFN21AE2598.seq Check: 5039 from: 1 to: 5866
pFN21AE2598 5866 bp
With 182 enzymes: SgfI *
January 12, 2016 11:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3209 1937
Size: 1272 4594
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2792 3929 5313 5581 1632
Size: 1160 1137 1384 268 1917
Fragments arranged by size:
1917 1384 1160 1137 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 2300 2606 2987 3609 5181 2300
Size: 306 381 622 1572 2985
Fragments arranged by size:
2985 1572 622 381 306
ApaLI G'TGCA_C
Cuts at: 2238 3923 5079 2238
Size: 1685 1156 3025
Fragments arranged by size:
3025 1685 1156
ApoI r'AATT_y
Cuts at: 1493 2044 2349 2392 2660 3175 3401 3553 5662
Size: 551 305 43 268 515 226 152 2109
Cuts at: 5662 1493
Size: 1697
Fragments arranged by size:
2109 1697 551 515 305 268 226 152
43
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 1870
Size: 73 1248 4545
Fragments arranged by size:
4545 1248 73
BamHI G'GATC_C
Cuts at: 2081 3196 3563 2081
Size: 1115 367 4384
Fragments arranged by size:
4384 1115 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3187 4642 618
Size: 325 217 627 1400 1455 1842
Fragments arranged by size:
1842 1455 1400 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3185 1391
Size: 1794 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3102 928
Size: 971 1203 3692
Fragments arranged by size:
3692 1203 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2199 3758 4968 2199
Size: 1559 1210 3097
Fragments arranged by size:
3097 1559 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4477 136
Size: 107 122 71 631 605 2805 1525
Fragments arranged by size:
2805 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 3283 3283
Size: 5866
Bme1580I G_kGCm'C
Cuts at: 1790 2242 3927 5083 1790
Size: 452 1685 1156 2573
Fragments arranged by size:
2573 1685 1156 452
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 3597 4555
Size: 416 53 83 399 24 858 1322 958
Cuts at: 4555 442
Size: 1753
Fragments arranged by size:
1753 1322 958 858 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2876 3009 4526 1239
Size: 993 644 133 1517 2579
Fragments arranged by size:
2579 1517 993 644 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2346 2651 3991 4856 5154 2346
Size: 305 1340 865 298 3058
Fragments arranged by size:
3058 1340 865 305 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4529 882
Size: 617 3030 2219
Fragments arranged by size:
3030 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 5860 5860
Size: 5866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2084 4299 4697 4971 5118 5529 2084
Size: 2215 398 274 147 411 2421
Fragments arranged by size:
2421 2215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5641
Fragments arranged by size:
5641 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5866
BseYI C'CCAG_C
Cuts at: 5069 5565 5069
Size: 496 5370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2805 1163
Size: 573 1069 4224
Fragments arranged by size:
4224 1069 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2242 2635 3927 4012 5083 2242
Size: 393 1292 85 1071 3025
Fragments arranged by size:
3025 1292 1071 393 85
BsmI GAATG_Cn'
Cuts at: 3377 3470 3377
Size: 93 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2242 2635 3185 3927 4012 5083 1391
Size: 399 452 393 550 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 452 399 393 85
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2530 2827 3221 844
Size: 1686 297 394 3489
Fragments arranged by size:
3489 1686 394 297
BsrBI CCG'CTC
Cuts at: 1394 1823 3753 1394
Size: 429 1930 3507
Fragments arranged by size:
3507 1930 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2011 4361 4535 65
Size: 1183 763 2350 174 1396
Fragments arranged by size:
2350 1396 1183 763 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4510 4697 1700
Size: 110 2700 187 2869
Fragments arranged by size:
2869 2700 187 110
BsrGI T'GTAC_A
Cuts at: 96 2577 2793 96
Size: 2481 216 3169
Fragments arranged by size:
3169 2481 216
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 3926 4938 3926
Size: 1012 4854
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2522 794
Size: 1728 4138
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 2081 3196 3563 3948 3965 5417 5503 5515
Size: 109 1115 367 385 17 1452 86 12
Cuts at: 5515 5861 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1115 385 367 346 109 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 4176 4196 3382
Size: 794 20 5052
Fragments arranged by size:
5052 794 20
ClaI AT'CG_AT
Cuts at: 1554 2052 1554
Size: 498 5368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 2169 3170 2169
Size: 1001 4865
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3313 4202 5831
Size: 54 1425 50 275 18 1483 889 1629
Cuts at: 5831 8
Size: 43
Fragments arranged by size:
1629 1483 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 3796 1353
Size: 719 1724 3423
Fragments arranged by size:
3423 1724 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2792 2876 3009 3929 4526 5313
Size: 393 600 560 84 133 920 597 787
Cuts at: 5313 5581 1239
Size: 268 1524
Fragments arranged by size:
1524 920 787 600 597 560 393 268
133 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2106 2106
Size: 5866
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
EcoRV GAT'ATC
Cuts at: 2598 2598
Size: 5866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2387 2822 2387
Size: 435 5431
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 5013 1678
Size: 3335 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2982 3014 3092 3110 3124
Size: 32 135 32 1410 32 78 18 14
Cuts at: 3124 3142 4507 4539 4581 4613 1373
Size: 18 1365 32 42 32 2626
Fragments arranged by size:
2626 1410 1365 135 78 42 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3210 3456 669
Size: 988 281 1272 246 3079
Fragments arranged by size:
3079 1272 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 2526 3465 2526
Size: 939 4927
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2766 2983 4980 5164 1305
Size: 1461 217 1997 184 2007
Fragments arranged by size:
2007 1997 1461 217 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4044
Fragments arranged by size:
4044 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3824 4183 4342 518
Size: 648 2658 359 159 2042
Fragments arranged by size:
2658 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3282 3959 5107 5352 1606
Size: 1676 677 1148 245 2120
Fragments arranged by size:
2120 1676 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
NdeI CA'TA_TG
Cuts at: 387 2637 387
Size: 2250 3616
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 3137 3224 4769 1132
Size: 97 1908 87 1545 2229
Fragments arranged by size:
2229 1908 1545 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5866
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2480 1108
Size: 1372 4494
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PstI C_TGCA'G
Cuts at: 830 2983 3218 830
Size: 2153 235 3478
Fragments arranged by size:
3478 2153 235
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 2949 2979 3214 4349 5030 5221 826
Size: 219 1904 30 235 1135 681 191 1471
Fragments arranged by size:
1904 1471 1135 681 235 219 191 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2361 2666 3970 4871 5133 820
Size: 197 926 418 305 1304 901 262 1553
Fragments arranged by size:
1553 1304 926 901 418 305 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3137 3224 1132
Size: 2005 87 3774
Fragments arranged by size:
3774 2005 87
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 2460 1517
Size: 943 4923
StyI C'CwwG_G
Cuts at: 513 1065 2004 3305 513
Size: 552 939 1301 3074
Fragments arranged by size:
3074 1301 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3893 3910 4063 4248 4693 325
Size: 1255 2313 17 153 185 445 1498
Fragments arranged by size:
2313 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 2793 4112
Size: 274 80 33 51 1443 600 216 1319
Cuts at: 4112 5824 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1319 600 274 216 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5826 5858 5826
Size: 32 5834
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI