Restriction map for
pFN21AE2588
(Circular) (Six-base) MAPSORT of: pFN21AE2588.seq Check: 8215 from: 1 to: 8134
pFN21AE2588 8134 bp
With 182 enzymes: SgfI *
September 18, 2015 18:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3237 5944 278
Size: 53 83 186 936 1701 2707 2468
Fragments arranged by size:
2707 2468 1701 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4726 5477 1937
Size: 2789 751 4594
Fragments arranged by size:
4594 2789 751
Acc65I G'GTAC_C
Cuts at: 2921 5455 2921
Size: 2534 5600
AclI AA'CG_TT
Cuts at: 1563 6261 6634 1563
Size: 4698 373 3063
Fragments arranged by size:
4698 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 3672 6197 7581 7849 1632
Size: 588 1452 2525 1384 268 1917
Fragments arranged by size:
2525 1917 1452 1384 588 268
AfeI AGC'GCT
Cuts at: 2193 2193
Size: 8134
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7937
AflIII A'CryG_T
Cuts at: 1203 2793 4540 4575 7033 8010 1203
Size: 1590 1747 35 2458 977 1327
Fragments arranged by size:
2458 1747 1590 1327 977 35
AgeI A'CCGG_T
Cuts at: 2981 6965 2981
Size: 3984 4150
AhdI GACnn_n'nnGTC
Cuts at: 4267 6863 4267
Size: 2596 5538
AleI CACnn'nnGTG
Cuts at: 1166 2603 4628 1166
Size: 1437 2025 4672
Fragments arranged by size:
4672 2025 1437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2289 3555 1184
Size: 1105 1266 5763
Fragments arranged by size:
5763 1266 1105
AlwNI CAG_nnn'CTG
Cuts at: 2175 2488 2531 2645 3517 4926 5877 7449 2175
Size: 313 43 114 872 1409 951 1572 2860
Fragments arranged by size:
2860 1572 1409 951 872 313 114 43
ApaI G_GGCC'C
Cuts at: 2090 4763 2090
Size: 2673 5461
ApaLI G'TGCA_C
Cuts at: 3500 6191 7347 3500
Size: 2691 1156 4287
Fragments arranged by size:
4287 2691 1156
ApoI r'AATT_y
Cuts at: 1493 4213 5443 5669 5821 7930 1493
Size: 2720 1230 226 152 2109 1697
Fragments arranged by size:
2720 2109 1697 1230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3321 6688 160
Size: 872 2289 3367 1606
Fragments arranged by size:
3367 2289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8134
AvaI C'yCGr_G
Cuts at: 1870 1943 3354 5459 1870
Size: 73 1411 2105 4545
Fragments arranged by size:
4545 2105 1411 73
BamHI G'GATC_C
Cuts at: 3310 5131 5464 5831 3310
Size: 1821 333 367 5613
Fragments arranged by size:
5613 1821 367 333
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2747 2921 4409 4679 5381
Size: 325 217 627 960 174 1488 270 702
Cuts at: 5381 5455 6910 618
Size: 74 1455 1842
Fragments arranged by size:
1842 1488 1455 960 702 627 325 270
217 174 74
BanII G_rGCy'C
Cuts at: 1391 2090 2115 2216 2466 2706 3132 3578 4027
Size: 699 25 101 250 240 426 446 449
Cuts at: 4027 4334 4755 4763 4887 4941 5015 5313 5453
Size: 307 421 8 124 54 74 298 140
Cuts at: 5453 1391
Size: 4072
Fragments arranged by size:
4072 699 449 446 426 421 307 298
250 240 140 124 101 74 54 25
8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2208 928
Size: 971 309 6854
Fragments arranged by size:
6854 971 309
BbvCI CC'TCA_GC
Cuts at: 2131 2549 3297 3453 3669 4141 2131
Size: 418 748 156 216 472 6124
Fragments arranged by size:
6124 748 472 418 216 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3894 3928 6325 6359 1903
Size: 34 1957 34 2397 34 3678
Fragments arranged by size:
3678 2397 1957 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6026 7236 6026
Size: 1210 6924
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8134
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4857 5019 6745
Size: 107 122 71 631 605 3185 162 1726
Cuts at: 6745 136
Size: 1525
Fragments arranged by size:
3185 1726 1525 631 605 162 122 107
71
BglII A'GATC_T
Cuts at: 8129 8129
Size: 8134
BlpI GC'TnA_GC
Cuts at: 2109 4881 5551 2109
Size: 2772 670 4692
Fragments arranged by size:
4692 2772 670
Bme1580I G_kGCm'C
Cuts at: 1790 2090 3354 3504 4583 4763 6195 7351 1790
Size: 300 1264 150 1079 180 1432 1156 2573
Fragments arranged by size:
2573 1432 1264 1156 1079 300 180 150
BmgBI CAC'GTC
Cuts at: 1318 2757 2809 1318
Size: 1439 52 6643
Fragments arranged by size:
6643 1439 52
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4000 5865 6823
Size: 416 53 83 399 24 2583 1865 958
Cuts at: 6823 442
Size: 1753
Fragments arranged by size:
2583 1865 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 3449 4330 2734
Size: 715 881 6538
Fragments arranged by size:
6538 881 715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2720 3444 3447 3642 4340 4739 4979 6794
Size: 1481 724 3 195 698 399 240 1815
Cuts at: 6794 1239
Size: 2579
Fragments arranged by size:
2579 1815 1481 724 698 399 240 195
3
Bpu10I CC'TnA_GC
Cuts at: 2131 2147 2283 2549 3297 3453 3669 4141 2131
Size: 16 136 266 748 156 216 472 6124
Fragments arranged by size:
6124 748 472 266 216 156 136 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2870 4217 4502 6259 7124 7422 2870
Size: 1347 285 1757 865 298 3582
Fragments arranged by size:
3582 1757 1347 865 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2722 4104 6797 882
Size: 617 1223 1382 2693 2219
Fragments arranged by size:
2693 2219 1382 1223 617
BsaAI yAC'GTr
Cuts at: 493 1204 2794 4578 5925 493
Size: 711 1590 1784 1347 2702
Fragments arranged by size:
2702 1784 1590 1347 711
BsaBI GATnn'nnATC
Cuts at: 8128 8128
Size: 8134
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3234 5941 6323 6958
Size: 53 83 186 936 1701 2707 382 635
Cuts at: 6958 275
Size: 1451
Fragments arranged by size:
2707 1701 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2981 3366 4889 5391 6567 6965 7239 7386 7797
Size: 385 1523 502 1176 398 274 147 411
Cuts at: 7797 2981
Size: 3318
Fragments arranged by size:
3318 1523 1176 502 411 398 385 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3986 4016 1178
Size: 30 165 30 2583 30 5296
Fragments arranged by size:
5296 2583 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2570 2984 4129 4607 4790 4949 1186
Size: 1384 414 1145 478 183 159 4371
Fragments arranged by size:
4371 1384 1145 478 414 183 159
BseYI C'CCAG_C
Cuts at: 2089 2114 2813 2960 3292 3664 3717 3746 3767
Size: 25 699 147 332 372 53 29 21
Cuts at: 3767 3887 3946 4895 5017 7337 7833 2089
Size: 120 59 949 122 2320 496 2390
Fragments arranged by size:
2390 2320 949 699 496 372 332 147
122 120 59 53 29 25 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2079 2249 2867 3016 4111 1163
Size: 573 343 170 618 149 1095 5186
Fragments arranged by size:
5186 1095 618 573 343 170 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3964 5290 5584 6345 7373 1540
Size: 275 186 1963 1326 294 761 1028 2301
Fragments arranged by size:
2301 1963 1326 1028 761 294 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 2289 2562 3132 3504 4755 4887 4914 5187
Size: 73 273 570 372 1251 132 27 273
Cuts at: 5187 6195 6280 7351 2216
Size: 1008 85 1071 2999
Fragments arranged by size:
2999 1251 1071 1008 570 372 273 273
132 85 73 27
BsmI GAATG_Cn'
Cuts at: 5645 5738 5645
Size: 93 8041
BsmBI CGTCTCn'nnnn_
Cuts at: 4701 4701
Size: 8134
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2115 2216 2289 2466 2562 2706
Size: 399 300 25 101 73 177 96 144
Cuts at: 2706 3132 3354 3504 3578 4027 4334 4583 4755
Size: 426 222 150 74 449 307 249 172
Cuts at: 4755 4763 4887 4914 4941 5015 5187 5313 5453
Size: 8 124 27 27 74 172 126 140
Cuts at: 5453 6195 6280 7351 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 426 399 307 300
249 222 177 172 172 150 144 140
126 124 101 96 85 74 74 73
27 27 25 8
BspEI T'CCGG_A
Cuts at: 3366 7797 3366
Size: 4431 3703
BspHI T'CATG_A
Cuts at: 6023 7753 6023
Size: 1730 6404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2109 3594 3989 5489 844
Size: 1265 1485 395 1500 3489
Fragments arranged by size:
3489 1500 1485 1265 395
BsrBI CCG'CTC
Cuts at: 1394 1823 3003 3727 4079 6021 1394
Size: 429 1180 724 352 1942 3507
Fragments arranged by size:
3507 1942 1180 724 429 352
BsrDI GCAATG_nn'
Cuts at: 65 1248 3247 6629 6803 65
Size: 1183 1999 3382 174 1396
Fragments arranged by size:
3382 1999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2981 4406 6778 6965 1700
Size: 110 1171 1425 2372 187 2869
Fragments arranged by size:
2869 2372 1425 1171 187 110
BsrGI T'GTAC_A
Cuts at: 96 2252 96
Size: 2156 5978
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8134
BssSI C'ACGA_G
Cuts at: 6194 7206 6194
Size: 1012 7122
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8134
BstBI TT'CG_AA
Cuts at: 6938 6938
Size: 8134
BstEII G'GTnAC_C
Cuts at: 1184 4615 1184
Size: 3431 4703
BstXI CCAn_nnnn'nTGG
Cuts at: 2744 3229 4903 4951 2744
Size: 485 1674 48 5927
Fragments arranged by size:
5927 1674 485 48
BstYI r'GATC_y
Cuts at: 1972 2433 3310 3363 3586 4668 5131 5464 5831
Size: 461 877 53 223 1082 463 333 367
Cuts at: 5831 6216 6233 7685 7771 7783 8129 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1082 877 463 461 385 367
346 333 223 86 53 17 12
BstZ17I GTA'TAC
Cuts at: 4727 4727
Size: 8134
Bsu36I CC'TnA_GG
Cuts at: 2350 4569 2350
Size: 2219 5915
BtgI C'CryG_G
Cuts at: 513 1065 2004 2306 2706 3076 3275 3777 3941
Size: 552 939 302 400 370 199 502 164
Cuts at: 3941 4161 513
Size: 220 4486
Fragments arranged by size:
4486 939 552 502 400 370 302 220
199 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2757 487
Size: 683 56 1531 5864
Fragments arranged by size:
5864 1531 683 56
BtsI GCAGTG_nn'
Cuts at: 2103 2266 2320 3537 3688 3829 4194 4332 5029
Size: 163 54 1217 151 141 365 138 697
Cuts at: 5029 5650 6444 6464 2103
Size: 621 794 20 3773
Fragments arranged by size:
3773 1217 794 697 621 365 163 151
141 138 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3229 3264 529
Size: 35 2665 35 5399
Fragments arranged by size:
5399 2665 35 35
DraI TTT'AAA
Cuts at: 5438 5438
Size: 8134
DraIII CAC_nnn'GTG
Cuts at: 3349 3349
Size: 8134
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7141 809
Size: 507 5825 1802
Fragments arranged by size:
5825 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2494 2575 2863
Size: 54 1425 50 275 18 664 81 288
Cuts at: 2863 3219 3340 3780 4158 4426 5581 6470 8099
Size: 356 121 440 378 268 1155 889 1629
Cuts at: 8099 8
Size: 43
Fragments arranged by size:
1629 1425 1155 889 664 440 378 356
288 275 268 121 81 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5581 1537
Size: 275 3769 4090
Fragments arranged by size:
4090 3769 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 2651 2902 3963 4321 5279 6064 1353
Size: 675 623 251 1061 358 958 785 3423
Fragments arranged by size:
3423 1061 958 785 675 623 358 251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6721 7095 7241 387
Size: 1064 138 5132 374 146 1280
Fragments arranged by size:
5132 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2214 3130 4753 4885 2214
Size: 916 1623 132 5463
Fragments arranged by size:
5463 1623 916 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2720 3444 3447 3642 3672 4340
Size: 393 588 500 724 3 195 30 668
Cuts at: 4340 4739 4979 6197 6794 7581 7849 1239
Size: 399 240 1218 597 787 268 1524
Fragments arranged by size:
1524 1218 787 724 668 597 588 500
399 393 268 240 195 30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 3462 2552
Size: 910 7224
EcoO109I rG'GnC_Cy
Cuts at: 2086 2485 2545 3289 4177 4759 4760 4830 5028
Size: 399 60 744 888 582 1 70 198
Cuts at: 5028 2086
Size: 5192
Fragments arranged by size:
5192 888 744 582 399 198 70 60
1
EcoRI G'AATT_C
Cuts at: 4213 5443 5821 4213
Size: 1230 378 6526
Fragments arranged by size:
6526 1230 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 4258 2422
Size: 1836 6298
FspI TGC'GCA
Cuts at: 6640 6640
Size: 8134
HaeII r_GCGC'y
Cuts at: 1678 2195 2677 3518 7281 1678
Size: 517 482 841 3763 2531
Fragments arranged by size:
3763 2531 841 517 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3348 3380 3913 3945 3986
Size: 32 135 32 1776 32 533 32 41
Cuts at: 3986 4018 4253 4285 5116 5148 5397 5404 5429
Size: 32 235 32 831 32 249 7 25
Cuts at: 5429 5436 6775 6807 6849 6881 1373
Size: 7 1339 32 42 32 2626
Fragments arranged by size:
2626 1776 1339 831 533 249 235 135
42 41 32 32 32 32 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4420 5478 5724 669
Size: 988 281 2482 1058 246 3079
Fragments arranged by size:
3079 2482 1058 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2454 748
Size: 1706 6428
HpaI GTT'AAC
Cuts at: 5724 5724
Size: 8134
KpnI G_GTAC'C
Cuts at: 2925 5459 2925
Size: 2534 5600
MfeI C'AATT_G
Cuts at: 5733 5733
Size: 8134
MluI A'CGCG_T
Cuts at: 8010 8010
Size: 8134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2884 4079 4493 4536 5102 5356 7248 7432
Size: 1579 1195 414 43 566 254 1892 184
Cuts at: 7432 1305
Size: 2007
Fragments arranged by size:
2007 1892 1579 1195 566 414 254 184
43
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2496 2865 3221 3342 3782
Size: 54 1425 343 664 369 356 121 440
Cuts at: 3782 4160 4428 10
Size: 378 268 3716
Fragments arranged by size:
3716 1425 664 440 378 369 356 343
268 121 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 4628 6092 6451 6610 518
Size: 648 1437 2025 1464 359 159 2042
Fragments arranged by size:
2042 2025 1464 1437 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2093 2308 3225 3296 3668 3750 3805 4000
Size: 487 215 917 71 372 82 55 195
Cuts at: 4000 4663 4877 5017 5550 6227 7375 7620 1606
Size: 663 214 140 533 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 663 533 487 372
245 215 214 195 140 82 71 55
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6643
Fragments arranged by size:
6643 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8134
NotI GC'GGCC_GC
Cuts at: 5581 5581
Size: 8134
NspI r_CATG'y
Cuts at: 1132 1229 3697 5492 7037 1132
Size: 97 2468 1795 1545 2229
Fragments arranged by size:
2468 2229 1795 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4172 1348
Size: 2824 5310
PciI A'CATG_T
Cuts at: 7033 7033
Size: 8134
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3880 4926 1108
Size: 2772 1046 4316
Fragments arranged by size:
4316 2772 1046
PfoI T'CCnGG_A
Cuts at: 2697 4223 4356 4377 4443 4671 4688 2697
Size: 1526 133 21 66 228 17 6143
Fragments arranged by size:
6143 1526 228 133 66 21 17
PmeI GTTT'AAAC
Cuts at: 5438 5438
Size: 8134
PmlI CAC'GTG
Cuts at: 4578 4578
Size: 8134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6203 6235 7716 7748 6203
Size: 32 1481 32 6589
Fragments arranged by size:
6589 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2485 3289 4830 2485
Size: 804 1541 5789
Fragments arranged by size:
5789 1541 804
PshAI GACnn'nnGTC
Cuts at: 1613 2726 1613
Size: 1113 7021
PsiI TTA'TAA
Cuts at: 5704 5704
Size: 8134
PspOMI G'GGCC_C
Cuts at: 2086 4759 2086
Size: 2673 5461
PstI C_TGCA'G
Cuts at: 830 3978 4662 5486 830
Size: 3148 684 824 3478
Fragments arranged by size:
3478 3148 824 684
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8134
PvuII CAG'CTG
Cuts at: 2093 3225 3296 3668 3805 4000 2093
Size: 1132 71 372 137 195 6227
Fragments arranged by size:
6227 1132 372 195 137 71
SacI G_AGCT'C
Cuts at: 2216 3132 4755 4887 2216
Size: 916 1623 132 5463
Fragments arranged by size:
5463 1623 916 132
SacII CC_GC'GG
Cuts at: 2309 2309
Size: 8134
SalI G'TCGA_C
Cuts at: 1936 5476 1936
Size: 3540 4594
SapI GCTCTTCn'nnn_
Cuts at: 2902 3963 2902
Size: 1061 7073
SbfI CC_TGCA'GG
Cuts at: 5486 5486
Size: 8134
ScaI AGT'ACT
Cuts at: 4988 6382 8094 4988
Size: 1394 1712 5028
Fragments arranged by size:
5028 1712 1394
SfcI C'TryA_G
Cuts at: 826 1045 3169 3974 4658 5482 6617 7298 7489
Size: 219 2124 805 684 824 1135 681 191
Cuts at: 7489 826
Size: 1471
Fragments arranged by size:
2124 1471 1135 824 805 684 681 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8134
SgrAI Cr'CCGG_yG
Cuts at: 1700 2981 1700
Size: 1281 6853
SmaI CCC'GGG
Cuts at: 1872 5461 1872
Size: 3589 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2885 4232 4481 6238 7139 7401
Size: 197 926 942 1347 249 1757 901 262
Cuts at: 7401 820
Size: 1553
Fragments arranged by size:
1757 1553 1347 942 926 901 262 249
197
SnaBI TAC'GTA
Cuts at: 493 5925 493
Size: 5432 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8134
SphI G_CATG'C
Cuts at: 1132 5492 1132
Size: 4360 3774
SspI AAT'ATT
Cuts at: 5 52 2248 6058 5
Size: 47 2196 3810 2081
Fragments arranged by size:
3810 2196 2081 47
StuI AGG'CCT
Cuts at: 1517 3909 5376 1517
Size: 2392 1467 4275
Fragments arranged by size:
4275 2392 1467
StyI C'CwwG_G
Cuts at: 513 1065 2004 3267 3314 3522 3543 3795 4155
Size: 552 939 1263 47 208 21 252 360
Cuts at: 4155 4320 4423 4429 4598 4944 5224 5573 513
Size: 165 103 6 169 346 280 349 3074
Fragments arranged by size:
3074 1263 939 552 360 349 346 280
252 208 169 165 103 47 21 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3013 3240 3978 4880 5369 6161 6178
Size: 1255 1433 227 738 902 489 792 17
Cuts at: 6178 6331 6516 6961 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1433 1255 902 792 738 489 445
227 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2252 4986 4998
Size: 274 80 33 51 1443 275 2734 12
Cuts at: 4998 6380 8092 96
Size: 1382 1712 138
Fragments arranged by size:
2734 1712 1443 1382 275 274 138 80
51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4532 4564 8094 8126 4532
Size: 32 3530 32 4540
Fragments arranged by size:
4540 3530 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3476 1316
Size: 2160 5974
XbaI T'CTAG_A
Cuts at: 3931 5470 3931
Size: 1539 6595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2410 2489 2646 3802 5014 2410
Size: 79 157 1156 1212 5530
Fragments arranged by size:
5530 1212 1156 157 79
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8134
XmaI C'CCGG_G
Cuts at: 1870 5459 1870
Size: 3589 4545
XmnI GAAnn'nnTTC
Cuts at: 4050 6263 4050
Size: 2213 5921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3235 5942 276
Size: 53 83 186 936 1701 2707 2468
Fragments arranged by size:
2707 2468 1701 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI
SfoI SrfI SwaI