Restriction map for
pFN21AE2584
(Circular) (Six-base) MAPSORT of: pFN21AE2584.seq Check: 7019 from: 1 to: 5323
pFN21AE2584 5323 bp
With 182 enzymes: SgfI *
August 7, 2015 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3133 278
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2085 2666 1937
Size: 148 581 4594
Fragments arranged by size:
4594 581 148
Acc65I G'GTAC_C
Cuts at: 2644 2644
Size: 5323
AclI AA'CG_TT
Cuts at: 1563 3450 3823 1563
Size: 1887 373 3063
Fragments arranged by size:
3063 1887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2409 3386 4770 5038 1632
Size: 777 977 1384 268 1917
Fragments arranged by size:
1917 1384 977 777 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5126
AflIII A'CryG_T
Cuts at: 1203 4222 5199 1203
Size: 3019 977 1327
Fragments arranged by size:
3019 1327 977
AgeI A'CCGG_T
Cuts at: 4154 4154
Size: 5323
AhdI GACnn_n'nnGTC
Cuts at: 4052 4052
Size: 5323
AleI CACnn'nnGTG
Cuts at: 1166 2093 1166
Size: 927 4396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5323
AlwNI CAG_nnn'CTG
Cuts at: 2135 2203 2281 2292 3066 4638 2135
Size: 68 78 11 774 1572 2820
Fragments arranged by size:
2820 1572 774 78 68 11
ApaLI G'TGCA_C
Cuts at: 3380 4536 3380
Size: 1156 4167
ApoI r'AATT_y
Cuts at: 1493 2035 2632 2858 3010 5119 1493
Size: 542 597 226 152 2109 1697
Fragments arranged by size:
2109 1697 597 542 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3877 160
Size: 872 2845 1606
Fragments arranged by size:
2845 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
AvaI C'yCGr_G
Cuts at: 1870 1943 2648 1870
Size: 73 705 4545
Fragments arranged by size:
4545 705 73
BamHI G'GATC_C
Cuts at: 2608 2653 3020 2608
Size: 45 367 4911
Fragments arranged by size:
4911 367 45
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2644 4099 618
Size: 325 217 627 857 1455 1842
Fragments arranged by size:
1842 1455 857 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2151 2235 2450 2642 1391
Size: 760 84 215 192 4072
Fragments arranged by size:
4072 760 215 192 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2393 2426 928
Size: 971 494 33 3825
Fragments arranged by size:
3825 971 494 33
BbvCI CC'TCA_GC
Cuts at: 2136 2578 2136
Size: 442 4881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3514 3548 1903
Size: 34 1577 34 3678
Fragments arranged by size:
3678 1577 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 4425 3215
Size: 1210 4113
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3934 136
Size: 107 122 71 631 605 2262 1525
Fragments arranged by size:
2262 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5318 5318
Size: 5323
BlpI GC'TnA_GC
Cuts at: 2157 2740 2157
Size: 583 4740
Bme1580I G_kGCm'C
Cuts at: 1790 3384 4540 1790
Size: 1594 1156 2573
Fragments arranged by size:
2573 1594 1156
BmgBI CAC'GTC
Cuts at: 1318 2302 1318
Size: 984 4339
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2362 3054 4012
Size: 416 53 83 399 24 945 692 958
Cuts at: 4012 442
Size: 1753
Fragments arranged by size:
1753 958 945 692 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3983 1239
Size: 2744 2579
Bpu10I CC'TnA_GC
Cuts at: 2136 2150 2578 2136
Size: 14 428 4881
Fragments arranged by size:
4881 428 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 3448 4313 4611 2219
Size: 1229 865 298 2931
Fragments arranged by size:
2931 1229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2314 3986 882
Size: 617 815 1672 2219
Fragments arranged by size:
2219 1672 815 617
BsaAI yAC'GTr
Cuts at: 493 1204 3114 493
Size: 711 1910 2702
Fragments arranged by size:
2702 1910 711
BsaBI GATnn'nnATC
Cuts at: 5317 5317
Size: 5323
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3130 3512 4147 275
Size: 53 83 186 936 1597 382 635 1451
Fragments arranged by size:
1597 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3756 4154 4428 4575 4986 3756
Size: 398 274 147 411 4093
Fragments arranged by size:
4093 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5098
Fragments arranged by size:
5098 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2156 2159 2254 1186
Size: 967 3 3 95 4255
Fragments arranged by size:
4255 967 95 3 3
BseYI C'CCAG_C
Cuts at: 2245 4526 5022 2245
Size: 2281 496 2546
Fragments arranged by size:
2546 2281 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2324 1163
Size: 573 588 4162
Fragments arranged by size:
4162 588 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2773 3534 4562 1540
Size: 275 186 772 761 1028 2301
Fragments arranged by size:
2301 1028 772 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2235 3384 3469 4540 2151
Size: 84 1149 85 1071 2934
Fragments arranged by size:
2934 1149 1071 85 84
BsmI GAATG_Cn'
Cuts at: 2834 2927 2834
Size: 93 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2235 2450 2642 3384 3469 4540
Size: 399 361 84 215 192 742 85 1071
Cuts at: 4540 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 361 215 192 85
84
BspEI T'CCGG_A
Cuts at: 4986 4986
Size: 5323
BspHI T'CATG_A
Cuts at: 3212 4942 3212
Size: 1730 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2296 2678 844
Size: 1452 382 3489
Fragments arranged by size:
3489 1452 382
BsrBI CCG'CTC
Cuts at: 1394 1823 3210 1394
Size: 429 1387 3507
Fragments arranged by size:
3507 1387 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3818 3992 65
Size: 1183 2570 174 1396
Fragments arranged by size:
2570 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 3967 4154 1700
Size: 110 205 1952 187 2869
Fragments arranged by size:
2869 1952 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5323
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5323
BssSI C'ACGA_G
Cuts at: 3383 4395 3383
Size: 1012 4311
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2302 794
Size: 1508 3815
BstBI TT'CG_AA
Cuts at: 4127 4127
Size: 5323
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5323
BstYI r'GATC_y
Cuts at: 1972 2608 2653 3020 3405 3422 4874 4960 4972
Size: 636 45 367 385 17 1452 86 12
Cuts at: 4972 5318 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 636 385 367 346 86 45
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3832
Fragments arranged by size:
3832 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4584
Fragments arranged by size:
4584 683 56
BtsI GCAGTG_nn'
Cuts at: 2566 2839 3633 3653 2566
Size: 273 794 20 4236
Fragments arranged by size:
4236 794 273 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5323
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5288
DraI TTT'AAA
Cuts at: 2627 2627
Size: 5323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2306 2497 2527 4330 809
Size: 507 990 191 30 1803 1802
Fragments arranged by size:
1803 1802 990 507 191 30
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2770 3659
Size: 54 1425 50 275 18 187 753 889
Cuts at: 3659 5288 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 753 275 187 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2770 1537
Size: 275 958 4090
Fragments arranged by size:
4090 958 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 3253 1353
Size: 816 1084 3423
Fragments arranged by size:
3423 1084 816
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2002 3910 4284 4430 387
Size: 1064 138 413 1908 374 146 1280
Fragments arranged by size:
1908 1280 1064 413 374 146 138
EcoICRI GAG'CTC
Cuts at: 2149 2233 2149
Size: 84 5239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2409 3386 3983 4770 5038 1239
Size: 393 777 977 597 787 268 1524
Fragments arranged by size:
1524 977 787 777 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2046 2046
Size: 5323
EcoO109I rG'GnC_Cy
Cuts at: 2200 2200
Size: 5323
EcoRI G'AATT_C
Cuts at: 2035 2632 3010 2035
Size: 597 378 4348
Fragments arranged by size:
4348 597 378
EcoRV GAT'ATC
Cuts at: 2026 2026
Size: 5323
FspI TGC'GCA
Cuts at: 3829 3829
Size: 5323
HaeII r_GCGC'y
Cuts at: 1678 2319 4470 1678
Size: 641 2151 2531
Fragments arranged by size:
2531 2151 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3964 3996 4038 4070 1373
Size: 32 135 32 2392 32 42 32 2626
Fragments arranged by size:
2626 2392 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2086 2667 2913 669
Size: 988 281 148 581 246 3079
Fragments arranged by size:
3079 988 581 281 246 148
HindIII A'AGCT_T
Cuts at: 748 2465 748
Size: 1717 3606
HpaI GTT'AAC
Cuts at: 2913 2913
Size: 5323
KpnI G_GTAC'C
Cuts at: 2648 2648
Size: 5323
MfeI C'AATT_G
Cuts at: 2922 2922
Size: 5323
MluI A'CGCG_T
Cuts at: 5199 5199
Size: 5323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 4437 4621 1305
Size: 1281 1851 184 2007
Fragments arranged by size:
2007 1851 1281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3501
Fragments arranged by size:
3501 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 3281 3640 3799 518
Size: 648 927 1188 359 159 2042
Fragments arranged by size:
2042 1188 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2429 2582 2739 3416 4564 4809 1606
Size: 823 153 157 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 677 245 157 153
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 5323
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3832
Fragments arranged by size:
3832 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5323
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 5323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5323
NotI GC'GGCC_GC
Cuts at: 2770 2770
Size: 5323
NspI r_CATG'y
Cuts at: 1132 1229 2681 4226 1132
Size: 97 1452 1545 2229
Fragments arranged by size:
2229 1545 1452 97
PasI CC'CwG_GG
Cuts at: 1348 2260 1348
Size: 912 4411
PciI A'CATG_T
Cuts at: 4222 4222
Size: 5323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 2292 1108
Size: 1095 89 4139
Fragments arranged by size:
4139 1095 89
PfoI T'CCnGG_A
Cuts at: 2285 2285
Size: 5323
PmeI GTTT'AAAC
Cuts at: 2627 2627
Size: 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 4905 4937 3392
Size: 32 1481 32 3778
Fragments arranged by size:
3778 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2200
Size: 5323
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5323
PsiI TTA'TAA
Cuts at: 2893 2893
Size: 5323
PstI C_TGCA'G
Cuts at: 830 2675 830
Size: 1845 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5323
PvuII CAG'CTG
Cuts at: 2429 2429
Size: 5323
SacI G_AGCT'C
Cuts at: 2151 2235 2151
Size: 84 5239
SalI G'TCGA_C
Cuts at: 1936 2084 2665 1936
Size: 148 581 4594
Fragments arranged by size:
4594 581 148
SbfI CC_TGCA'GG
Cuts at: 2675 2675
Size: 5323
ScaI AGT'ACT
Cuts at: 3571 5283 3571
Size: 1712 3611
SfcI C'TryA_G
Cuts at: 826 1045 2671 3806 4487 4678 826
Size: 219 1626 1135 681 191 1471
Fragments arranged by size:
1626 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5323
SmaI CCC'GGG
Cuts at: 1872 2650 1872
Size: 778 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 3427 4328 4590 820
Size: 197 926 291 1193 901 262 1553
Fragments arranged by size:
1553 1193 926 901 291 262 197
SnaBI TAC'GTA
Cuts at: 493 3114 493
Size: 2621 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5323
SphI G_CATG'C
Cuts at: 1132 2681 1132
Size: 1549 3774
SspI AAT'ATT
Cuts at: 5 52 3247 5
Size: 47 3195 2081
Fragments arranged by size:
3195 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5323
StyI C'CwwG_G
Cuts at: 513 1065 2004 2166 2211 2762 513
Size: 552 939 162 45 551 3074
Fragments arranged by size:
3074 939 552 551 162 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2526 2531 3350 3367 3520 3705 4150
Size: 1255 946 5 819 17 153 185 445
Cuts at: 4150 325
Size: 1498
Fragments arranged by size:
1498 1255 946 819 445 185 153 17
5
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3569 5281 96
Size: 274 80 33 51 1443 1592 1712 138
Fragments arranged by size:
1712 1592 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5283 5315 5283
Size: 32 5291
Tth111I GACn'n_nGTC
Cuts at: 1316 2368 1316
Size: 1052 4271
XbaI T'CTAG_A
Cuts at: 2659 2659
Size: 5323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5323
XmaI C'CCGG_G
Cuts at: 1870 2648 1870
Size: 778 4545
XmnI GAAnn'nnTTC
Cuts at: 3452 3452
Size: 5323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3131 276
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI