Restriction map for
pFN21AE2579
(Circular) (Six-base) MAPSORT of: pFN21AE2579.seq Check: 5880 from: 1 to: 6370
pFN21AE2579 6370 bp
With 182 enzymes: SgfI *
June 25, 2015 16:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3478 3478
Size: 6370
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2484 4180 278
Size: 53 83 186 936 948 1696 2468
Fragments arranged by size:
2468 1696 948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2937 3713 1937
Size: 1000 776 4594
Fragments arranged by size:
4594 1000 776
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3173 4433 5817 6085 1632
Size: 1541 1260 1384 268 1917
Fragments arranged by size:
1917 1541 1384 1260 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6173
AflIII A'CryG_T
Cuts at: 1203 2296 2857 5269 6246 1203
Size: 1093 561 2412 977 1327
Fragments arranged by size:
2412 1327 1093 977 561
AgeI A'CCGG_T
Cuts at: 5201 5201
Size: 6370
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 2297 1166
Size: 1131 5239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3532 1184
Size: 2348 4022
AlwNI CAG_nnn'CTG
Cuts at: 3493 4113 5685 3493
Size: 620 1572 4178
Fragments arranged by size:
4178 1572 620
ApaLI G'TGCA_C
Cuts at: 4427 5583 4427
Size: 1156 5214
ApoI r'AATT_y
Cuts at: 1493 2748 3679 3905 4057 6166 1493
Size: 1255 931 226 152 2109 1697
Fragments arranged by size:
2109 1697 1255 931 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4924 160
Size: 872 3892 1606
Fragments arranged by size:
3892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 3695 1870
Size: 73 1752 4545
Fragments arranged by size:
4545 1752 73
BamHI G'GATC_C
Cuts at: 2328 3700 4067 2328
Size: 1372 367 4631
Fragments arranged by size:
4631 1372 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2564 3691 5146 618
Size: 325 217 627 777 1127 1455 1842
Fragments arranged by size:
1842 1455 1127 777 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3045 3689 1391
Size: 1654 644 4072
Fragments arranged by size:
4072 1654 644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2476 2998 928
Size: 971 577 522 4300
Fragments arranged by size:
4300 971 577 522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2293 2327 4561 4595 1903
Size: 34 356 34 2234 34 3678
Fragments arranged by size:
3678 2234 356 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4262 5472 4262
Size: 1210 5160
BclI T'GATC_A
Cuts at: 1548 2242 2439 1548
Size: 694 197 5479
Fragments arranged by size:
5479 694 197
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2542 2646 4981
Size: 107 122 71 631 605 870 104 2335
Cuts at: 4981 136
Size: 1525
Fragments arranged by size:
2335 1525 870 631 605 122 107 104
71
BglII A'GATC_T
Cuts at: 2316 6365 2316
Size: 4049 2321
BlpI GC'TnA_GC
Cuts at: 2120 3787 2120
Size: 1667 4703
Bme1580I G_kGCm'C
Cuts at: 1790 2567 2594 4431 5587 1790
Size: 777 27 1837 1156 2573
Fragments arranged by size:
2573 1837 1156 777 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6370
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4101 5059 442
Size: 416 53 83 399 24 2684 958 1753
Fragments arranged by size:
2684 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 5030 1239
Size: 860 2931 2579
Fragments arranged by size:
2931 2579 860
Bpu10I CC'TnA_GC
Cuts at: 2891 2891
Size: 6370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2365 4495 5360 5658 2365
Size: 2130 865 298 3077
Fragments arranged by size:
3077 2130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3170 5033 882
Size: 617 1671 1863 2219
Fragments arranged by size:
2219 1863 1671 617
BsaAI yAC'GTr
Cuts at: 493 1204 2299 2860 4161 493
Size: 711 1095 561 1301 2702
Fragments arranged by size:
2702 1301 1095 711 561
BsaBI GATnn'nnATC
Cuts at: 6364 6364
Size: 6370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2481 2805 3201 4177
Size: 53 83 186 936 948 324 396 976
Cuts at: 4177 4559 5194 275
Size: 382 635 1451
Fragments arranged by size:
1451 976 948 936 635 396 382 324
186 83 53
BsaWI w'CCGG_w
Cuts at: 2197 4803 5201 5475 5622 6033 2197
Size: 2606 398 274 147 411 2534
Fragments arranged by size:
2606 2534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2176 2206 1178
Size: 30 165 30 773 30 5342
Fragments arranged by size:
5342 773 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2852 1186
Size: 1666 4704
BseYI C'CCAG_C
Cuts at: 3283 3292 3493 3590 5573 6069 3283
Size: 9 201 97 1983 496 3584
Fragments arranged by size:
3584 1983 496 201 97 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2753 3506 1163
Size: 573 1017 753 4027
Fragments arranged by size:
4027 1017 753 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2712 3820 4581 5609 1540
Size: 275 186 711 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 711 275 186
BsiHKAI G_wGCw'C
Cuts at: 3045 4431 4516 5587 3045
Size: 1386 85 1071 3828
Fragments arranged by size:
3828 1386 1071 85
BsmI GAATG_Cn'
Cuts at: 3881 3974 3881
Size: 93 6277
BsmBI CGTCTCn'nnnn_
Cuts at: 3208 3208
Size: 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2567 2594 3045 3689 4431 4516 5587
Size: 399 777 27 451 644 742 85 1071
Cuts at: 5587 1391
Size: 2174
Fragments arranged by size:
2174 1071 777 742 644 451 399 85
27
BspEI T'CCGG_A
Cuts at: 6033 6033
Size: 6370
BspHI T'CATG_A
Cuts at: 2346 4259 5989 2346
Size: 1913 1730 2727
Fragments arranged by size:
2727 1913 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2694 3478 3725 844
Size: 1850 784 247 3489
Fragments arranged by size:
3489 1850 784 247
BsrBI CCG'CTC
Cuts at: 1394 1823 2192 2998 4257 1394
Size: 429 369 806 1259 3507
Fragments arranged by size:
3507 1259 806 429 369
BsrDI GCAATG_nn'
Cuts at: 65 1248 4865 5039 65
Size: 1183 3617 174 1396
Fragments arranged by size:
3617 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3215 5014 5201 1700
Size: 110 1405 1799 187 2869
Fragments arranged by size:
2869 1799 1405 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6370
BssSI C'ACGA_G
Cuts at: 2063 2160 4430 5442 2063
Size: 97 2270 1012 2991
Fragments arranged by size:
2991 2270 1012 97
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6370
BstBI TT'CG_AA
Cuts at: 2143 5174 2143
Size: 3031 3339
BstEII G'GTnAC_C
Cuts at: 1184 2203 1184
Size: 1019 5351
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 6370
BstYI r'GATC_y
Cuts at: 1972 2046 2316 2328 3700 4067 4452 4469 5921
Size: 74 270 12 1372 367 385 17 1452
Cuts at: 5921 6007 6019 6365 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1372 385 367 346 270 86
74 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 3079 3079
Size: 6370
BtgI C'CryG_G
Cuts at: 513 1065 2004 2107 2583 2712 2721 3207 3545
Size: 552 939 103 476 129 9 486 338
Cuts at: 3545 513
Size: 3338
Fragments arranged by size:
3338 939 552 486 476 338 129 103
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2932 3256 3343 3370 487
Size: 683 56 1706 324 87 27 3487
Fragments arranged by size:
3487 1706 683 324 87 56 27
BtsI GCAGTG_nn'
Cuts at: 2537 3447 3886 4680 4700 2537
Size: 910 439 794 20 4207
Fragments arranged by size:
4207 910 794 439 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6335
DraI TTT'AAA
Cuts at: 3674 3674
Size: 6370
DraIII CAC_nnn'GTG
Cuts at: 2598 2598
Size: 6370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5377 809
Size: 507 4061 1802
Fragments arranged by size:
4061 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2709 3213 3817
Size: 54 1425 50 275 18 879 504 604
Cuts at: 3817 4706 6335 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 879 604 504 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2709 3817 1537
Size: 275 897 1108 4090
Fragments arranged by size:
4090 1108 897 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2498 2696 3005 3050 3266 4300 1353
Size: 1145 198 309 45 216 1034 3423
Fragments arranged by size:
3423 1145 1034 309 216 198 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4957 5331 5477 387
Size: 1064 138 3368 374 146 1280
Fragments arranged by size:
3368 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3043 3043
Size: 6370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 3173 4433 5030 5817 6085 1239
Size: 393 467 1074 1260 597 787 268 1524
Fragments arranged by size:
1524 1260 1074 787 597 467 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2441 2834 2441
Size: 393 5977
EcoO109I rG'GnC_Cy
Cuts at: 2434 3370 3512 3526 2434
Size: 936 142 14 5278
Fragments arranged by size:
5278 936 142 14
EcoRI G'AATT_C
Cuts at: 3679 4057 3679
Size: 378 5992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3439 3439
Size: 6370
FspI TGC'GCA
Cuts at: 2303 4876 2303
Size: 2573 3797
FspAI rTGC'GCAy
Cuts at: 2303 2303
Size: 6370
HaeII r_GCGC'y
Cuts at: 1678 3071 5517 1678
Size: 1393 2446 2531
Fragments arranged by size:
2531 2446 1393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3369 3401 5011 5043 5085
Size: 32 135 32 1797 32 1610 32 42
Cuts at: 5085 5117 1373
Size: 32 2626
Fragments arranged by size:
2626 1797 1610 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3714 3960 669
Size: 988 281 1776 246 3079
Fragments arranged by size:
3079 1776 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6370
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2502 5484 5668 1305
Size: 1197 2982 184 2007
Fragments arranged by size:
2982 2007 1197 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4548
Fragments arranged by size:
4548 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2757 4328 4687 4846 518
Size: 648 1131 460 1571 359 159 2042
Fragments arranged by size:
2042 1571 1131 648 460 359 159
MspA1I CmG'CkG
Cuts at: 1606 3786 4463 5611 5856 1606
Size: 2180 677 1148 245 2120
Fragments arranged by size:
2180 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 3217 3217
Size: 6370
NcoI C'CATG_G
Cuts at: 513 1065 2004 2107 513
Size: 552 939 103 4776
Fragments arranged by size:
4776 939 552 103
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6370
NgoMIV G'CCGG_C
Cuts at: 3215 3215
Size: 6370
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 3728 5273 1132
Size: 97 2499 1545 2229
Fragments arranged by size:
2499 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2911 1348
Size: 1563 4807
PciI A'CATG_T
Cuts at: 5269 5269
Size: 6370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6370
PfoI T'CCnGG_A
Cuts at: 2391 2514 2676 3408 2391
Size: 123 162 732 5353
Fragments arranged by size:
5353 732 162 123
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PmlI CAC'GTG
Cuts at: 2299 2860 2299
Size: 561 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 5952 5984 4439
Size: 32 1481 32 4825
Fragments arranged by size:
4825 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3526 3526
Size: 6370
PshAI GACnn'nnGTC
Cuts at: 1613 3242 1613
Size: 1629 4741
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PstI C_TGCA'G
Cuts at: 830 2475 3722 830
Size: 1645 1247 3478
Fragments arranged by size:
3478 1645 1247
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
SacI G_AGCT'C
Cuts at: 3045 3045
Size: 6370
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SanDI GG'GwC_CC
Cuts at: 3526 3526
Size: 6370
SapI GCTCTTCn'nnn_
Cuts at: 2696 3005 3050 3266 2696
Size: 309 45 216 5800
Fragments arranged by size:
5800 309 216 45
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 4618 6330 4618
Size: 1712 4658
SexAI A'CCwGG_T
Cuts at: 3302 3302
Size: 6370
SfcI C'TryA_G
Cuts at: 826 1045 2471 3013 3718 4853 5534 5725 826
Size: 219 1426 542 705 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 705 681 542 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 3697 1872
Size: 1825 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2380 4474 5375 5637 820
Size: 197 926 437 2094 901 262 1553
Fragments arranged by size:
2094 1553 926 901 437 262 197
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 3728 1132
Size: 2596 3774
SspI AAT'ATT
Cuts at: 5 52 4294 5
Size: 47 4242 2081
Fragments arranged by size:
4242 2081 47
StuI AGG'CCT
Cuts at: 1517 2267 1517
Size: 750 5620
StyI C'CwwG_G
Cuts at: 513 1065 2004 2107 3342 3520 3619 3809 513
Size: 552 939 103 1235 178 99 190 3074
Fragments arranged by size:
3074 1235 939 552 190 178 103 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4397 4414 4567 4752 5197 325
Size: 1255 2817 17 153 185 445 1498
Fragments arranged by size:
2817 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4616 6328 96
Size: 274 80 33 51 1443 2639 1712 138
Fragments arranged by size:
2639 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2176 2208 2451 2483 3550 3582 6330 6362 2176
Size: 32 243 32 1067 32 2748 32 2184
Fragments arranged by size:
2748 2184 1067 243 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2807 1316
Size: 1491 4879
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 3695 1870
Size: 1825 4545
XmnI GAAnn'nnTTC
Cuts at: 2606 3422 4499 2606
Size: 816 1077 4477
Fragments arranged by size:
4477 1077 816
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2482 4178 276
Size: 53 83 186 936 948 1696 2468
Fragments arranged by size:
2468 1696 948 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I EcoRV FseI KasI NarI
NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII
SacII SfiI SfoI SrfI SwaI XcmI