Restriction map for
pFN21AE2578
(Circular) (Six-base) MAPSORT of: pFN21AE2578.seq Check: 4974 from: 1 to: 8209
pFN21AE2578 8209 bp
With 182 enzymes: SgfI *
June 25, 2015 16:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2099 2365 3003 4354 4377 4439 4738 5276 2099
Size: 266 638 1351 23 62 299 538 5032
Fragments arranged by size:
5032 1351 638 538 299 266 62 23
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6019 278
Size: 53 83 186 936 4483 2468
Fragments arranged by size:
4483 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2742 3360 4757 5552 1937
Size: 805 618 1397 795 4594
Fragments arranged by size:
4594 1397 805 795 618
Acc65I G'GTAC_C
Cuts at: 5530 5530
Size: 8209
AclI AA'CG_TT
Cuts at: 1563 6336 6709 1563
Size: 4773 373 3063
Fragments arranged by size:
4773 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 6272 7656 7924 1632
Size: 4640 1384 268 1917
Fragments arranged by size:
4640 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8012
AflIII A'CryG_T
Cuts at: 1203 3621 4284 7108 8085 1203
Size: 2418 663 2824 977 1327
Fragments arranged by size:
2824 2418 1327 977 663
AgeI A'CCGG_T
Cuts at: 7040 7040
Size: 8209
AhdI GACnn_n'nnGTC
Cuts at: 6938 6938
Size: 8209
AleI CACnn'nnGTG
Cuts at: 1166 2495 3342 1166
Size: 1329 847 6033
Fragments arranged by size:
6033 1329 847
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2889 3202 4136 1184
Size: 1705 313 934 5257
Fragments arranged by size:
5257 1705 934 313
AlwNI CAG_nnn'CTG
Cuts at: 3202 3574 5952 7524 3202
Size: 372 2378 1572 3887
Fragments arranged by size:
3887 2378 1572 372
ApaI G_GGCC'C
Cuts at: 2183 5276 5501 2183
Size: 3093 225 4891
Fragments arranged by size:
4891 3093 225
ApaLI G'TGCA_C
Cuts at: 3187 3443 6266 7422 3187
Size: 256 2823 1156 3974
Fragments arranged by size:
3974 2823 1156 256
ApoI r'AATT_y
Cuts at: 1493 4336 5518 5744 5896 8005 1493
Size: 2843 1182 226 152 2109 1697
Fragments arranged by size:
2843 2109 1697 1182 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6763 160
Size: 872 5731 1606
Fragments arranged by size:
5731 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8209
AvaI C'yCGr_G
Cuts at: 1870 1943 2103 3321 3555 3720 4890 5534 1870
Size: 73 160 1218 234 165 1170 644 4545
Fragments arranged by size:
4545 1218 1170 644 234 165 160 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2274 2307 3554 3587 2274
Size: 33 1247 33 6896
Fragments arranged by size:
6896 1247 33 33
BamHI G'GATC_C
Cuts at: 4000 5539 5906 4000
Size: 1539 367 6303
Fragments arranged by size:
6303 1539 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2190 2323 2335 2361
Size: 325 217 627 304 99 133 12 26
Cuts at: 2361 2724 2873 3665 3842 4418 5530 6985 618
Size: 363 149 792 177 576 1112 1455 1842
Fragments arranged by size:
1842 1455 1112 792 627 576 363 325
304 217 177 149 133 99 26 12
BanII G_rGCy'C
Cuts at: 1391 2183 2586 3225 4545 5276 5302 5501 5528
Size: 792 403 639 1320 731 26 199 27
Cuts at: 5528 1391
Size: 4072
Fragments arranged by size:
4072 1320 792 731 639 403 199 27
26
BbeI G_GCGC'C
Cuts at: 2095 2327 2365 3669 2095
Size: 232 38 1304 6635
Fragments arranged by size:
6635 1304 232 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3078 3099 4033 4483 928
Size: 971 1179 21 934 450 4654
Fragments arranged by size:
4654 1179 971 934 450 21
BbvCI CC'TCA_GC
Cuts at: 2316 2532 2789 2316
Size: 216 257 7736
Fragments arranged by size:
7736 257 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2990 3024 3701 3735 6400 6434 1903
Size: 34 1053 34 677 34 2665 34 3678
Fragments arranged by size:
3678 2665 1053 677 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2275 6101 7311 2275
Size: 3826 1210 3173
Fragments arranged by size:
3826 3173 1210
BclI T'GATC_A
Cuts at: 1548 3916 1548
Size: 2368 5841
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2546 3537 3609
Size: 107 122 71 631 605 874 991 72
Cuts at: 3609 3701 3810 6820 136
Size: 92 109 3010 1525
Fragments arranged by size:
3010 1525 991 874 631 605 122 109
107 92 72 71
BglII A'GATC_T
Cuts at: 3111 3276 8204 3111
Size: 165 4928 3116
Fragments arranged by size:
4928 3116 165
BlpI GC'TnA_GC
Cuts at: 4743 5626 4743
Size: 883 7326
Bme1580I G_kGCm'C
Cuts at: 1790 2183 2195 2340 2729 3191 3447 3845 4033
Size: 393 12 145 389 462 256 398 188
Cuts at: 4033 4225 4421 5276 5501 6270 7426 1790
Size: 192 196 855 225 769 1156 2573
Fragments arranged by size:
2573 1156 855 769 462 398 393 389
256 225 196 192 188 145 12
BmgBI CAC'GTC
Cuts at: 1318 4870 1318
Size: 3552 4657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3178 5270 5940
Size: 416 53 83 399 24 1761 2092 670
Cuts at: 5940 6898 442
Size: 958 1753
Fragments arranged by size:
2092 1761 1753 958 670 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3394 3394
Size: 8209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 5009 5453 6869 1239
Size: 1193 2577 444 1416 2579
Fragments arranged by size:
2579 2577 1416 1193 444
Bpu10I CC'TnA_GC
Cuts at: 2316 2532 2789 3887 2316
Size: 216 257 1098 6638
Fragments arranged by size:
6638 1098 257 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3614 3995 6334 7199 7497 3614
Size: 381 2339 865 298 4326
Fragments arranged by size:
4326 2339 865 381 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2957 4634 6872 882
Size: 617 1458 1677 2238 2219
Fragments arranged by size:
2238 2219 1677 1458 617
BsaAI yAC'GTr
Cuts at: 493 1204 2644 3389 6000 493
Size: 711 1440 745 2611 2702
Fragments arranged by size:
2702 2611 1440 745 711
BsaBI GATnn'nnATC
Cuts at: 3275 8203 3275
Size: 4928 3281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2092 2208 2324 2362
Size: 53 83 186 936 559 116 116 38
Cuts at: 2362 3453 3666 4614 6016 6398 7033 275
Size: 1091 213 948 1402 382 635 1451
Fragments arranged by size:
1451 1402 1091 948 936 635 559 382
213 186 116 116 83 53 38
BsaWI w'CCGG_w
Cuts at: 2217 4836 6642 7040 7314 7461 7872 2217
Size: 2619 1806 398 274 147 411 2554
Fragments arranged by size:
2619 2554 1806 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 4005 4035 1178
Size: 30 165 30 1002 30 1570 30 5352
Fragments arranged by size:
5352 1570 1002 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2750 3701 4823 4832 5081 1186
Size: 1564 951 1122 9 249 4314
Fragments arranged by size:
4314 1564 1122 951 249 9
BseYI C'CCAG_C
Cuts at: 2162 2248 2650 3045 3753 4567 4576 5501 7412
Size: 86 402 395 708 814 9 925 1911
Cuts at: 7412 7908 2162
Size: 496 2463
Fragments arranged by size:
2463 1911 925 814 708 496 402 395
86 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2104 2393 2690 2881 2902 4341 4481
Size: 573 368 289 297 191 21 1439 140
Cuts at: 4481 1163
Size: 4891
Fragments arranged by size:
4891 1439 573 368 297 289 191 140
21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5377 5659 6420 7448 1540
Size: 275 186 3376 282 761 1028 2301
Fragments arranged by size:
3376 2301 1028 761 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 3191 3225 3447 5232 6270 6355 7426 3191
Size: 34 222 1785 1038 85 1071 3974
Fragments arranged by size:
3974 1785 1071 1038 222 85 34
BsmI GAATG_Cn'
Cuts at: 5720 5813 5720
Size: 93 8116
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2195 2340 2586 2729 3191 3225
Size: 399 393 12 145 246 143 462 34
Cuts at: 3225 3447 3845 4033 4225 4421 4545 5232 5276
Size: 222 398 188 192 196 124 687 44
Cuts at: 5276 5302 5501 5528 6270 6355 7426 1391
Size: 26 199 27 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 687 462 399 398 393
246 222 199 196 192 188 145 143
124 85 44 34 27 26 12
BspEI T'CCGG_A
Cuts at: 2217 7872 2217
Size: 5655 2554
BspHI T'CATG_A
Cuts at: 3207 6098 7828 3207
Size: 2891 1730 3588
Fragments arranged by size:
3588 2891 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 2365 2662 3003 4354 4377 4439 4738
Size: 1255 266 297 341 1351 23 62 299
Cuts at: 4738 5276 5564 844
Size: 538 288 3489
Fragments arranged by size:
3489 1351 1255 538 341 299 297 288
266 62 23
BsrBI CCG'CTC
Cuts at: 1394 1823 3022 4215 5071 5163 6096 1394
Size: 429 1199 1193 856 92 933 3507
Fragments arranged by size:
3507 1199 1193 933 856 429 92
BsrDI GCAATG_nn'
Cuts at: 65 1248 2077 4275 4909 4956 6704 6878 65
Size: 1183 829 2198 634 47 1748 174 1396
Fragments arranged by size:
2198 1748 1396 1183 829 634 174 47
BsrFI r'CCGG_y
Cuts at: 1700 1810 2601 3942 6853 7040 1700
Size: 110 791 1341 2911 187 2869
Fragments arranged by size:
2911 2869 1341 791 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8209
BssSI C'ACGA_G
Cuts at: 6269 7281 6269
Size: 1012 7197
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8209
BstBI TT'CG_AA
Cuts at: 7013 7013
Size: 8209
BstEII G'GTnAC_C
Cuts at: 1184 5386 1184
Size: 4202 4007
BstXI CCAn_nnnn'nTGG
Cuts at: 2632 3382 3517 3733 4870 2632
Size: 750 135 216 1137 5971
Fragments arranged by size:
5971 1137 750 216 135
BstYI r'GATC_y
Cuts at: 1972 3111 3276 3970 4000 4101 5539 5906 6291
Size: 1139 165 694 30 101 1438 367 385
Cuts at: 6291 6308 7760 7846 7858 8204 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1438 1139 694 385 367 346
165 101 86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 3266 3864 4179 5125 3266
Size: 598 315 946 6350
Fragments arranged by size:
6350 946 598 315
BtgI C'CryG_G
Cuts at: 513 1065 2095 2842 2968 3057 3604 3732 4618
Size: 552 1030 747 126 89 547 128 886
Cuts at: 4618 5391 513
Size: 773 3331
Fragments arranged by size:
3331 1030 886 773 747 552 547 128
126 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2731 5023 487
Size: 683 56 1505 2292 3673
Fragments arranged by size:
3673 2292 1505 683 56
BtsI GCAGTG_nn'
Cuts at: 2268 2673 3189 3259 3337 3445 3563 4356 5172
Size: 405 516 70 78 108 118 793 816
Cuts at: 5172 5512 5725 6519 6539 2268
Size: 340 213 794 20 3938
Fragments arranged by size:
3938 816 794 793 516 405 340 213
118 108 78 70 20
ClaI AT'CG_AT
Cuts at: 1554 3900 1554
Size: 2346 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4570 4605 529
Size: 35 4006 35 4133
Fragments arranged by size:
4133 4006 35 35
DraI TTT'AAA
Cuts at: 5513 5513
Size: 8209
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7216 809
Size: 507 5900 1802
Fragments arranged by size:
5900 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 3507 3538
Size: 54 1425 50 275 18 573 1104 31
Cuts at: 3538 3702 4479 4521 5656 6545 8174 8
Size: 164 777 42 1135 889 1629 43
Fragments arranged by size:
1629 1425 1135 1104 889 777 573 275
164 54 50 43 42 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 5656 1537
Size: 275 3844 4090
Fragments arranged by size:
4090 3844 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6139 1353
Size: 4786 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2603 3791 6796 7170 7316 387
Size: 1064 138 1014 1188 3005 374 146 1280
Fragments arranged by size:
3005 1280 1188 1064 1014 374 146 138
EcoICRI GAG'CTC
Cuts at: 3223 3223
Size: 8209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 5009 5453 6272 6869 7656 7924
Size: 393 800 2577 444 819 597 787 268
Cuts at: 7924 1239
Size: 1524
Fragments arranged by size:
2577 1524 819 800 787 597 444 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 4412 5402 4412
Size: 990 7219
EcoO109I rG'GnC_Cy
Cuts at: 2180 4183 4571 5368 5497 5498 2180
Size: 2003 388 797 129 1 4891
Fragments arranged by size:
4891 2003 797 388 129 1
EcoRI G'AATT_C
Cuts at: 5518 5896 5518
Size: 378 7831
FspI TGC'GCA
Cuts at: 3195 6715 3195
Size: 3520 4689
HaeII r_GCGC'y
Cuts at: 1678 2095 2327 2365 2419 2685 2950 3669 4730
Size: 417 232 38 54 266 265 719 1061
Cuts at: 4730 4900 7356 1678
Size: 170 2456 2531
Fragments arranged by size:
2531 2456 1061 719 417 266 265 232
170 54 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3473 3505 3955 3987 5433
Size: 32 135 32 1901 32 450 32 1446
Cuts at: 5433 5465 6850 6882 6924 6956 1373
Size: 32 1385 32 42 32 2626
Fragments arranged by size:
2626 1901 1446 1385 450 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4758 5553 5799 669
Size: 988 281 2820 795 246 3079
Fragments arranged by size:
3079 2820 988 795 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8209
HpaI GTT'AAC
Cuts at: 5799 5799
Size: 8209
KasI G'GCGC_C
Cuts at: 2091 2323 2361 3665 2091
Size: 232 38 1304 6635
Fragments arranged by size:
6635 1304 232 38
KpnI G_GTAC'C
Cuts at: 5534 5534
Size: 8209
MfeI C'AATT_G
Cuts at: 5808 5808
Size: 8209
MluI A'CGCG_T
Cuts at: 3621 8085 3621
Size: 4464 3745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7323 7507 1305
Size: 6018 184 2007
Fragments arranged by size:
6018 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3509 3704 4481 4523 10
Size: 54 1425 343 1677 195 777 42 3696
Fragments arranged by size:
3696 1677 1425 777 343 195 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2591 2630 2771 3062 3132 3184
Size: 648 1329 96 39 141 291 70 52
Cuts at: 3184 3342 3419 4091 5114 5223 6167 6526 6685
Size: 158 77 672 1023 109 944 359 159
Cuts at: 6685 518
Size: 2042
Fragments arranged by size:
2042 1329 1023 944 672 648 359 291
159 158 141 109 96 77 70 52
39
MspA1I CmG'CkG
Cuts at: 1606 2303 2428 2495 2793 2970 3435 3715 3922
Size: 697 125 67 298 177 465 280 207
Cuts at: 3922 4667 5083 5431 5625 6302 7450 7695 1606
Size: 745 416 348 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 745 697 677 465 416 348
298 280 245 207 194 177 125 67
NarI GG'CG_CC
Cuts at: 2092 2324 2362 3666 2092
Size: 232 38 1304 6635
Fragments arranged by size:
6635 1304 232 38
NcoI C'CATG_G
Cuts at: 513 1065 3057 3732 4618 513
Size: 552 1992 675 886 4104
Fragments arranged by size:
4104 1992 886 675 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8209
NotI GC'GGCC_GC
Cuts at: 5656 5656
Size: 8209
NspI r_CATG'y
Cuts at: 1132 1229 2776 3418 4288 4465 4742 5567 7112
Size: 97 1547 642 870 177 277 825 1545
Cuts at: 7112 1132
Size: 2229
Fragments arranged by size:
2229 1547 1545 870 825 642 277 177
97
PasI CC'CwG_GG
Cuts at: 1348 2269 1348
Size: 921 7288
PciI A'CATG_T
Cuts at: 4284 7108 4284
Size: 2824 5385
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 3605 4624 4885 1108
Size: 902 1595 1019 261 4432
Fragments arranged by size:
4432 1595 1019 902 261
PfoI T'CCnGG_A
Cuts at: 2145 3776 3989 2145
Size: 1631 213 6365
Fragments arranged by size:
6365 1631 213
PmeI GTTT'AAAC
Cuts at: 5513 5513
Size: 8209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5057 5089 6278 6310 7791 7823 5057
Size: 32 1189 32 1481 32 5443
Fragments arranged by size:
5443 1481 1189 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4571 5368 4571
Size: 797 7412
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8209
PsiI TTA'TAA
Cuts at: 5779 5779
Size: 8209
PspOMI G'GGCC_C
Cuts at: 2179 5272 5497 2179
Size: 3093 225 4891
Fragments arranged by size:
4891 3093 225
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 8177
PstI C_TGCA'G
Cuts at: 830 3000 3570 3714 4374 4436 4672 5561 830
Size: 2170 570 144 660 62 236 889 3478
Fragments arranged by size:
3478 2170 889 660 570 236 144 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8209
PvuII CAG'CTG
Cuts at: 3922 4667 5083 5431 3922
Size: 745 416 348 6700
Fragments arranged by size:
6700 745 416 348
SacI G_AGCT'C
Cuts at: 3225 3225
Size: 8209
SacII CC_GC'GG
Cuts at: 2971 2971
Size: 8209
SalI G'TCGA_C
Cuts at: 1936 4756 5551 1936
Size: 2820 795 4594
Fragments arranged by size:
4594 2820 795
SanDI GG'GwC_CC
Cuts at: 4571 5368 4571
Size: 797 7412
SbfI CC_TGCA'GG
Cuts at: 5561 5561
Size: 8209
ScaI AGT'ACT
Cuts at: 3986 4448 6457 8169 3986
Size: 462 2009 1712 4026
Fragments arranged by size:
4026 2009 1712 462
SexAI A'CCwGG_T
Cuts at: 5403 5403
Size: 8209
SfcI C'TryA_G
Cuts at: 826 1045 2996 3566 3710 4370 4432 4668 4787
Size: 219 1951 570 144 660 62 236 119
Cuts at: 4787 4823 5557 6692 7373 7564 826
Size: 36 734 1135 681 191 1471
Fragments arranged by size:
1951 1471 1135 734 681 660 570 236
219 191 144 119 62 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 3537 3701 3537
Size: 164 8045
SfoI GGC'GCC
Cuts at: 2093 2325 2363 3667 2093
Size: 232 38 1304 6635
Fragments arranged by size:
6635 1304 232 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8209
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8209
SmaI CCC'GGG
Cuts at: 1872 3323 4892 5536 1872
Size: 1451 1569 644 4545
Fragments arranged by size:
4545 1569 1451 644
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3629 3974 6313 7214 7476 820
Size: 197 926 1686 345 2339 901 262 1553
Fragments arranged by size:
2339 1686 1553 926 901 345 262 197
SnaBI TAC'GTA
Cuts at: 493 6000 493
Size: 5507 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8209
SphI G_CATG'C
Cuts at: 1132 2776 4742 5567 1132
Size: 1644 1966 825 3774
Fragments arranged by size:
3774 1966 1644 825
SrfI GCCC'GGGC
Cuts at: 3323 3323
Size: 8209
SspI AAT'ATT
Cuts at: 5 52 6133 5
Size: 47 6081 2081
Fragments arranged by size:
6081 2081 47
StuI AGG'CCT
Cuts at: 1517 2954 3797 1517
Size: 1437 843 5929
Fragments arranged by size:
5929 1437 843
StyI C'CwwG_G
Cuts at: 513 1065 3057 3457 3732 3783 3792 4618 4863
Size: 552 1992 400 275 51 9 826 245
Cuts at: 4863 5418 5648 513
Size: 555 230 3074
Fragments arranged by size:
3074 1992 826 555 552 400 275 245
230 51 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2176 2620 3860 5391 6236 6253 6406
Size: 1255 596 444 1240 1531 845 17 153
Cuts at: 6406 6591 7036 325
Size: 185 445 1498
Fragments arranged by size:
1531 1498 1255 1240 845 596 445 444
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3984 4155 4446
Size: 274 80 33 51 1443 2007 171 291
Cuts at: 4446 6455 8167 96
Size: 2009 1712 138
Fragments arranged by size:
2009 2007 1712 1443 291 274 171 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2612 2644 2911 2943 8169 8201 2612
Size: 32 267 32 5226 32 2620
Fragments arranged by size:
5226 2620 267 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3134 1316
Size: 1818 6391
XbaI T'CTAG_A
Cuts at: 5545 5545
Size: 8209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5200 5255 5200
Size: 55 8154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8209
XmaI C'CCGG_G
Cuts at: 1870 3321 4890 5534 1870
Size: 1451 1569 644 4545
Fragments arranged by size:
4545 1569 1451 644
XmnI GAAnn'nnTTC
Cuts at: 2393 6338 2393
Size: 3945 4264
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6017 276
Size: 53 83 186 936 4483 2468
Fragments arranged by size:
4483 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI RsrII SapI SwaI