Restriction map for
pFN21AE2574
(Circular) (Six-base) MAPSORT of: pFN21AE2574.seq Check: 8611 from: 1 to: 6028
pFN21AE2574 6028 bp
With 182 enzymes: SgfI *
April 21, 2015 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2584 2621 3193 2584
Size: 37 572 5419
Fragments arranged by size:
5419 572 37
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3838 278
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3371 1937
Size: 1434 4594
Acc65I G'GTAC_C
Cuts at: 3349 3349
Size: 6028
AclI AA'CG_TT
Cuts at: 1563 4155 4528 1563
Size: 2592 373 3063
Fragments arranged by size:
3063 2592 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2435 3016 4091 5475 5743 1632
Size: 803 581 1075 1384 268 1917
Fragments arranged by size:
1917 1384 1075 803 581 268
AfeI AGC'GCT
Cuts at: 2016 2016
Size: 6028
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5831
AflIII A'CryG_T
Cuts at: 1203 2774 4927 5904 1203
Size: 1571 2153 977 1327
Fragments arranged by size:
2153 1571 1327 977
AgeI A'CCGG_T
Cuts at: 4859 4859
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 4757 4757
Size: 6028
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6028
AlwNI CAG_nnn'CTG
Cuts at: 3771 5343 3771
Size: 1572 4456
ApaI G_GGCC'C
Cuts at: 2050 2050
Size: 6028
ApaLI G'TGCA_C
Cuts at: 4085 5241 4085
Size: 1156 4872
ApoI r'AATT_y
Cuts at: 1493 2304 2388 2439 2751 3094 3164 3337 3563
Size: 811 84 51 312 343 70 173 226
Cuts at: 3563 3715 5824 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 811 343 312 226 173 152
84 70 51
AseI AT'TA_AT
Cuts at: 160 1032 4582 160
Size: 872 3550 1606
Fragments arranged by size:
3550 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
AvaI C'yCGr_G
Cuts at: 1870 1943 2488 3353 1870
Size: 73 545 865 4545
Fragments arranged by size:
4545 865 545 73
AvrII C'CTAG_G
Cuts at: 2935 2935
Size: 6028
BamHI G'GATC_C
Cuts at: 3358 3725 3358
Size: 367 5661
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2033 3349 4804 618
Size: 325 217 627 221 25 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 221 217 25
BanII G_rGCy'C
Cuts at: 1391 2050 3347 1391
Size: 659 1297 4072
Fragments arranged by size:
4072 1297 659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4219 4253 1903
Size: 34 2282 34 3678
Fragments arranged by size:
3678 2282 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3920 5130 3920
Size: 1210 4818
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4639 136
Size: 107 122 71 631 605 2967 1525
Fragments arranged by size:
2967 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6023 6023
Size: 6028
BlpI GC'TnA_GC
Cuts at: 3445 3445
Size: 6028
Bme1580I G_kGCm'C
Cuts at: 1790 2050 4089 5245 1790
Size: 260 2039 1156 2573
Fragments arranged by size:
2573 2039 1156 260
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6028
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3759 4717 442
Size: 416 53 83 399 24 2342 958 1753
Fragments arranged by size:
2342 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 4688 1239
Size: 1220 2229 2579
Fragments arranged by size:
2579 2229 1220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4153 5018 5316 4153
Size: 865 298 4865
Fragments arranged by size:
4865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4691 882
Size: 617 3192 2219
Fragments arranged by size:
3192 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3819 493
Size: 711 2615 2702
Fragments arranged by size:
2702 2615 711
BsaBI GATnn'nnATC
Cuts at: 6022 6022
Size: 6028
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3835 4217 4852 275
Size: 53 83 186 936 2302 382 635 1451
Fragments arranged by size:
2302 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2908 4461 4859 5133 5280 5691 2908
Size: 1553 398 274 147 411 3245
Fragments arranged by size:
3245 1553 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5803
Fragments arranged by size:
5803 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 1186
Size: 1165 4863
BseYI C'CCAG_C
Cuts at: 5231 5727 5231
Size: 496 5532
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2373 2799 2803 1163
Size: 573 637 426 4 4388
Fragments arranged by size:
4388 637 573 426 4
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3478 4239 5267 1540
Size: 275 186 1477 761 1028 2301
Fragments arranged by size:
2301 1477 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2203 4089 4174 5245 2203
Size: 1886 85 1071 2986
Fragments arranged by size:
2986 1886 1071 85
BsmI GAATG_Cn'
Cuts at: 2715 3539 3632 2715
Size: 824 93 5111
Fragments arranged by size:
5111 824 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2203 3347 4089 4174 5245 1391
Size: 399 260 153 1144 742 85 1071 2174
Fragments arranged by size:
2174 1144 1071 742 399 260 153 85
BspEI T'CCGG_A
Cuts at: 5691 5691
Size: 6028
BspHI T'CATG_A
Cuts at: 3917 5647 3917
Size: 1730 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2584 2621 2868 3193 3383 844
Size: 1740 37 247 325 190 3489
Fragments arranged by size:
3489 1740 325 247 190 37
BsrBI CCG'CTC
Cuts at: 1394 1823 3915 1394
Size: 429 2092 3507
Fragments arranged by size:
3507 2092 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4523 4697 65
Size: 1183 3275 174 1396
Fragments arranged by size:
3275 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2063 2922 4672 4859 1700
Size: 110 253 859 1750 187 2869
Fragments arranged by size:
2869 1750 859 253 187 110
BsrGI T'GTAC_A
Cuts at: 96 2344 96
Size: 2248 3780
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6028
BssSI C'ACGA_G
Cuts at: 4088 5100 4088
Size: 1012 5016
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2996 794
Size: 2202 3826
BstBI TT'CG_AA
Cuts at: 4832 4832
Size: 6028
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6028
BstYI r'GATC_y
Cuts at: 1972 3358 3725 4110 4127 5579 5665 5677 6023
Size: 1386 367 385 17 1452 86 12 346
Cuts at: 6023 1972
Size: 1977
Fragments arranged by size:
1977 1452 1386 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2495 2495
Size: 6028
BtgI C'CryG_G
Cuts at: 513 1065 2004 2050 513
Size: 552 939 46 4491
Fragments arranged by size:
4491 939 552 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5289
Fragments arranged by size:
5289 683 56
BtsI GCAGTG_nn'
Cuts at: 2408 3544 4338 4358 2408
Size: 1136 794 20 4078
Fragments arranged by size:
4078 1136 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2386 2421 529
Size: 35 1822 35 4136
Fragments arranged by size:
4136 1822 35 35
DraI TTT'AAA
Cuts at: 3004 3325 3332 3004
Size: 321 7 5700
Fragments arranged by size:
5700 321 7
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5035 809
Size: 507 3719 1802
Fragments arranged by size:
3719 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3475 4364 5993
Size: 54 1425 50 275 18 1645 889 1629
Cuts at: 5993 8
Size: 43
Fragments arranged by size:
1645 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3475 1537
Size: 275 1663 4090
Fragments arranged by size:
4090 1663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3958 1353
Size: 2605 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2187 4615 4989 5135 387
Size: 1064 138 598 2428 374 146 1280
Fragments arranged by size:
2428 1280 1064 598 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2435 2459 3016 4091 4688 5475 5743
Size: 393 803 24 557 1075 597 787 268
Cuts at: 5743 1239
Size: 1524
Fragments arranged by size:
1524 1075 803 787 597 557 393 268
24
EcoNI CCTnn'n_nnAGG
Cuts at: 2934 2934
Size: 6028
EcoO109I rG'GnC_Cy
Cuts at: 2455 2981 2455
Size: 526 5502
EcoRI G'AATT_C
Cuts at: 3337 3715 3337
Size: 378 5650
FspI TGC'GCA
Cuts at: 4534 4534
Size: 6028
HaeII r_GCGC'y
Cuts at: 1678 2018 2136 5175 1678
Size: 340 118 3039 2531
Fragments arranged by size:
3039 2531 340 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2190 2222 4669 4701 4743
Size: 32 135 32 618 32 2447 32 42
Cuts at: 4743 4775 1373
Size: 32 2626
Fragments arranged by size:
2626 2447 618 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3372 3618 669
Size: 988 281 1434 246 3079
Fragments arranged by size:
3079 1434 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2411 748
Size: 1663 4365
HpaI GTT'AAC
Cuts at: 3618 3618
Size: 6028
KpnI G_GTAC'C
Cuts at: 3353 3353
Size: 6028
MfeI C'AATT_G
Cuts at: 2721 3627 2721
Size: 906 5122
MluI A'CGCG_T
Cuts at: 5904 5904
Size: 6028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5142 5326 1305
Size: 3837 184 2007
Fragments arranged by size:
3837 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4206
Fragments arranged by size:
4206 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2283 3248 3986 4345 4504 518
Size: 648 1117 965 738 359 159 2042
Fragments arranged by size:
2042 1117 965 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2113 3444 4121 5269 5514 1606
Size: 446 61 1331 677 1148 245 2120
Fragments arranged by size:
2120 1331 1148 677 446 245 61
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4537
Fragments arranged by size:
4537 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6028
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6028
NotI GC'GGCC_GC
Cuts at: 3475 3475
Size: 6028
NspI r_CATG'y
Cuts at: 1132 1229 2778 3132 3386 4931 1132
Size: 97 1549 354 254 1545 2229
Fragments arranged by size:
2229 1549 1545 354 254 97
PasI CC'CwG_GG
Cuts at: 1348 2075 1348
Size: 727 5301
PciI A'CATG_T
Cuts at: 2774 4927 2774
Size: 2153 3875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2573 1108
Size: 1465 4563
PmeI GTTT'AAAC
Cuts at: 3332 3332
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4097 4129 5610 5642 4097
Size: 32 1481 32 4483
Fragments arranged by size:
4483 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6028
PsiI TTA'TAA
Cuts at: 2313 2729 3598 2313
Size: 416 869 4743
Fragments arranged by size:
4743 869 416
PspOMI G'GGCC_C
Cuts at: 2046 2046
Size: 6028
PstI C_TGCA'G
Cuts at: 830 3380 830
Size: 2550 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6028
SacII CC_GC'GG
Cuts at: 2053 2053
Size: 6028
SalI G'TCGA_C
Cuts at: 1936 3370 1936
Size: 1434 4594
SbfI CC_TGCA'GG
Cuts at: 3380 3380
Size: 6028
ScaI AGT'ACT
Cuts at: 2372 4276 5988 2372
Size: 1904 1712 2412
Fragments arranged by size:
2412 1904 1712
SfcI C'TryA_G
Cuts at: 826 1045 3376 4511 5192 5383 826
Size: 219 2331 1135 681 191 1471
Fragments arranged by size:
2331 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
SgrAI Cr'CCGG_yG
Cuts at: 1700 2063 1700
Size: 363 5665
SmaI CCC'GGG
Cuts at: 1872 2490 3355 1872
Size: 618 865 4545
Fragments arranged by size:
4545 865 618
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4132 5033 5295 820
Size: 197 926 2189 901 262 1553
Fragments arranged by size:
2189 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3819 493
Size: 3326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6028
SphI G_CATG'C
Cuts at: 1132 3386 1132
Size: 2254 3774
SspI AAT'ATT
Cuts at: 5 52 2902 3952 5
Size: 47 2850 1050 2081
Fragments arranged by size:
2850 2081 1050 47
StuI AGG'CCT
Cuts at: 1517 2472 1517
Size: 955 5073
StyI C'CwwG_G
Cuts at: 513 1065 2004 2935 2954 3467 513
Size: 552 939 931 19 513 3074
Fragments arranged by size:
3074 939 931 552 513 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3235 4055 4072 4225 4410 4855 325
Size: 1255 1655 820 17 153 185 445 1498
Fragments arranged by size:
1655 1498 1255 820 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2344 2370 4274
Size: 274 80 33 51 1443 367 26 1904
Cuts at: 4274 5986 96
Size: 1712 138
Fragments arranged by size:
1904 1712 1443 367 274 138 80 51
33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2812 2844 5988 6020 2812
Size: 32 3144 32 2820
Fragments arranged by size:
3144 2820 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6028
XbaI T'CTAG_A
Cuts at: 3364 3364
Size: 6028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2630 3046 2630
Size: 416 5612
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6028
XmaI C'CCGG_G
Cuts at: 1870 2488 3353 1870
Size: 618 865 4545
Fragments arranged by size:
4545 865 618
XmnI GAAnn'nnTTC
Cuts at: 2439 4157 2439
Size: 1718 4310
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3836 276
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI