Restriction map for
pFN21AE2568
(Circular) (Six-base) MAPSORT of: pFN21AE2568.seq Check: 461 from: 1 to: 5500
pFN21AE2568 5500 bp
With 182 enzymes: SgfI *
March 23, 2015 11:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3310 278
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2843 1937
Size: 906 4594
Acc65I G'GTAC_C
Cuts at: 2821 2821
Size: 5500
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3563 4947 5215 1632
Size: 1931 1384 268 1917
Fragments arranged by size:
1931 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5303
AflIII A'CryG_T
Cuts at: 1203 4399 5376 1203
Size: 3196 977 1327
Fragments arranged by size:
3196 1327 977
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 4229 4229
Size: 5500
AleI CACnn'nnGTG
Cuts at: 1166 2591 1166
Size: 1425 4075
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2210 2242 2210
Size: 32 5468
AlwNI CAG_nnn'CTG
Cuts at: 2454 3243 4815 2454
Size: 789 1572 3139
Fragments arranged by size:
3139 1572 789
ApaI G_GGCC'C
Cuts at: 2600 2600
Size: 5500
ApaLI G'TGCA_C
Cuts at: 2069 2124 3557 4713 2069
Size: 55 1433 1156 2856
Fragments arranged by size:
2856 1433 1156 55
ApoI r'AATT_y
Cuts at: 1493 2734 2809 3035 3187 5296 1493
Size: 1241 75 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 226 152 75
AseI AT'TA_AT
Cuts at: 160 1032 4054 160
Size: 872 3022 1606
Fragments arranged by size:
3022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2600 2825 1870
Size: 73 657 225 4545
Fragments arranged by size:
4545 657 225 73
BamHI G'GATC_C
Cuts at: 2830 3197 2830
Size: 367 5133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2352 2700 2821 4276 618
Size: 325 217 627 565 348 121 1455 1842
Fragments arranged by size:
1842 1455 627 565 348 325 217 121
BanII G_rGCy'C
Cuts at: 1391 2600 2819 1391
Size: 1209 219 4072
Fragments arranged by size:
4072 1209 219
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2380 928
Size: 971 481 4048
Fragments arranged by size:
4048 971 481
BbvCI CC'TCA_GC
Cuts at: 2433 2433
Size: 5500
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3691 3725 1903
Size: 34 1754 34 3678
Fragments arranged by size:
3678 1754 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2255 3392 4602 2255
Size: 1137 1210 3153
Fragments arranged by size:
3153 1210 1137
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4111 136
Size: 107 122 71 631 605 2439 1525
Fragments arranged by size:
2439 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2292 5495 2292
Size: 3203 2297
BlpI GC'TnA_GC
Cuts at: 2189 2917 2189
Size: 728 4772
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2128 2209 2442 2600 2703 3561 4717
Size: 283 55 81 233 158 103 858 1156
Cuts at: 4717 1790
Size: 2573
Fragments arranged by size:
2573 1156 858 283 233 158 103 81
55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3231 4189 442
Size: 416 53 83 399 24 1814 958 1753
Fragments arranged by size:
1814 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2124 2480 2786 4160 1239
Size: 885 356 306 1374 2579
Fragments arranged by size:
2579 1374 885 356 306
Bpu10I CC'TnA_GC
Cuts at: 2023 2433 2023
Size: 410 5090
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4490 4788 3625
Size: 865 298 4337
Fragments arranged by size:
4337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4163 882
Size: 617 2664 2219
Fragments arranged by size:
2664 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3291 493
Size: 711 2087 2702
Fragments arranged by size:
2702 2087 711
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3307 3689 4324 275
Size: 53 83 186 936 1774 382 635 1451
Fragments arranged by size:
1774 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3933 4331 4605 4752 5163 3933
Size: 398 274 147 411 4270
Fragments arranged by size:
4270 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2210 2240 2517 2547 1178
Size: 30 165 30 807 30 277 30 4131
Fragments arranged by size:
4131 807 277 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2396 1186
Size: 1042 168 4290
Fragments arranged by size:
4290 1042 168
BseYI C'CCAG_C
Cuts at: 2148 2343 2541 2743 4703 5199 2148
Size: 195 198 202 1960 496 2449
Fragments arranged by size:
2449 1960 496 202 198 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 2699 1163
Size: 573 441 522 3964
Fragments arranged by size:
3964 573 522 441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2950 3711 4739 1540
Size: 275 186 949 761 1028 2301
Fragments arranged by size:
2301 1028 949 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2128 3561 3646 4717 2073
Size: 55 1433 85 1071 2856
Fragments arranged by size:
2856 1433 1071 85 55
BsmI GAATG_Cn'
Cuts at: 3011 3104 3011
Size: 93 5407
BsmBI CGTCTCn'nnnn_
Cuts at: 2156 2786 2156
Size: 630 4870
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2128 2209 2442 2600 2703 2819
Size: 399 283 55 81 233 158 103 116
Cuts at: 2819 3561 3646 4717 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 233 158 116
103 85 81 55
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 3389 5119 3389
Size: 1730 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2855 844
Size: 2011 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2188 3387 1394
Size: 429 365 1199 3507
Fragments arranged by size:
3507 1199 429 365
BsrDI GCAATG_nn'
Cuts at: 65 1248 3995 4169 65
Size: 1183 2747 174 1396
Fragments arranged by size:
2747 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2553 4144 4331 1700
Size: 110 255 488 1591 187 2869
Fragments arranged by size:
2869 1591 488 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5500
BssSI C'ACGA_G
Cuts at: 2210 3560 4572 2210
Size: 1350 1012 3138
Fragments arranged by size:
3138 1350 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5500
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5500
BstYI r'GATC_y
Cuts at: 1972 2292 2830 3197 3582 3599 5051 5137 5149
Size: 320 538 367 385 17 1452 86 12
Cuts at: 5149 5495 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 538 385 367 346 320 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2080 513
Size: 552 1015 3933
Fragments arranged by size:
3933 1015 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4761
Fragments arranged by size:
4761 683 56
BtsI GCAGTG_nn'
Cuts at: 2054 2071 2684 3016 3810 3830 2054
Size: 17 613 332 794 20 3724
Fragments arranged by size:
3724 794 613 332 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5465
DraI TTT'AAA
Cuts at: 2804 2804
Size: 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4507 809
Size: 507 3191 1802
Fragments arranged by size:
3191 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2947 3836 5465
Size: 54 1425 50 275 18 1117 889 1629
Cuts at: 5465 8
Size: 43
Fragments arranged by size:
1629 1425 1117 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2947 1537
Size: 275 1135 4090
Fragments arranged by size:
4090 1135 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3430 1353
Size: 2077 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4087 4461 4607 387
Size: 1064 138 2498 374 146 1280
Fragments arranged by size:
2498 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2480 2786 3563 4160 4947 5215
Size: 393 492 356 306 777 597 787 268
Cuts at: 5215 1239
Size: 1524
Fragments arranged by size:
1524 787 777 597 492 393 356 306
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2768 2768
Size: 5500
EcoO109I rG'GnC_Cy
Cuts at: 2597 2597
Size: 5500
EcoRI G'AATT_C
Cuts at: 2734 2809 3187 2734
Size: 75 378 5047
Fragments arranged by size:
5047 378 75
EcoRV GAT'ATC
Cuts at: 2584 2584
Size: 5500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 5500
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 2095 2497 4647 1678
Size: 417 402 2150 2531
Fragments arranged by size:
2531 2150 417 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 4141 4173 4215
Size: 32 135 32 712 32 1825 32 42
Cuts at: 4215 4247 1373
Size: 32 2626
Fragments arranged by size:
2626 1825 712 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2844 3090 669
Size: 988 281 906 246 3079
Fragments arranged by size:
3079 988 906 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5500
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KpnI G_GTAC'C
Cuts at: 2825 2825
Size: 5500
MfeI C'AATT_G
Cuts at: 3099 3099
Size: 5500
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2688 4614 4798 1305
Size: 1383 1926 184 2007
Fragments arranged by size:
2007 1926 1383 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3678
Fragments arranged by size:
3678 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 3458 3817 3976 518
Size: 648 1425 867 359 159 2042
Fragments arranged by size:
2042 1425 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2491 2685 2916 3593 4741 4986 1606
Size: 591 294 194 231 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 591 294 245 231 194
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4948
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5500
NotI GC'GGCC_GC
Cuts at: 2947 2947
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2505 2858 4403 1132
Size: 97 1276 353 1545 2229
Fragments arranged by size:
2229 1545 1276 353 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5500
PciI A'CATG_T
Cuts at: 4399 4399
Size: 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2330 2578 1108
Size: 1222 248 4030
Fragments arranged by size:
4030 1222 248
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2210 2242 2766 2798 3569 3601 5082 5114 2210
Size: 32 524 32 771 32 1481 32 2596
Fragments arranged by size:
2596 1481 771 524 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2319 1613
Size: 706 4794
PsiI TTA'TAA
Cuts at: 3070 3070
Size: 5500
PspOMI G'GGCC_C
Cuts at: 2596 2596
Size: 5500
PstI C_TGCA'G
Cuts at: 830 2046 2490 2679 2852 830
Size: 1216 444 189 173 3478
Fragments arranged by size:
3478 1216 444 189 173
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5500
PvuII CAG'CTG
Cuts at: 2197 2197
Size: 5500
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SbfI CC_TGCA'GG
Cuts at: 2852 2852
Size: 5500
ScaI AGT'ACT
Cuts at: 3748 5460 3748
Size: 1712 3788
SexAI A'CCwGG_T
Cuts at: 2133 2133
Size: 5500
SfcI C'TryA_G
Cuts at: 826 1045 2042 2486 2532 2675 2848 3983 4664
Size: 219 997 444 46 143 173 1135 681
Cuts at: 4664 4855 826
Size: 191 1471
Fragments arranged by size:
1471 1135 997 681 444 219 191 173
143 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2602 2827 1872
Size: 730 225 4545
Fragments arranged by size:
4545 730 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3604 4505 4767 820
Size: 197 926 1661 901 262 1553
Fragments arranged by size:
1661 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3291 493
Size: 2798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2858 1132
Size: 1726 3774
SspI AAT'ATT
Cuts at: 5 52 3424 5
Size: 47 3372 2081
Fragments arranged by size:
3372 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5500
StyI C'CwwG_G
Cuts at: 513 1065 2424 2939 513
Size: 552 1359 515 3074
Fragments arranged by size:
3074 1359 552 515
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 2566 3527 3544 3697 3882 4327
Size: 1255 828 158 961 17 153 185 445
Cuts at: 4327 325
Size: 1498
Fragments arranged by size:
1498 1255 961 828 445 185 158 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3746 5458 96
Size: 274 80 33 51 1443 1769 1712 138
Fragments arranged by size:
1769 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 5468
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5500
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2455 2455
Size: 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2600 2825 1870
Size: 730 225 4545
Fragments arranged by size:
4545 730 225
XmnI GAAnn'nnTTC
Cuts at: 3629 3629
Size: 5500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3308 276
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI