Restriction map for
pFN21AE2565
(Circular) (Six-base) MAPSORT of: pFN21AE2565.seq Check: 5742 from: 1 to: 8161
pFN21AE2565 8161 bp
With 182 enzymes: SgfI *
March 23, 2015 11:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5971 278
Size: 53 83 186 936 4435 2468
Fragments arranged by size:
4435 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5504 1937
Size: 3567 4594
Acc65I G'GTAC_C
Cuts at: 5482 5482
Size: 8161
AclI AA'CG_TT
Cuts at: 1563 6288 6661 1563
Size: 4725 373 3063
Fragments arranged by size:
4725 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2759 2976 3975 4221 4820 5349 5367 6224
Size: 1127 217 999 246 599 529 18 857
Cuts at: 6224 7608 7876 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1127 999 857 599 529 268
246 217 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7964
AflIII A'CryG_T
Cuts at: 1203 3519 7060 8037 1203
Size: 2316 3541 977 1327
Fragments arranged by size:
3541 2316 1327 977
AgeI A'CCGG_T
Cuts at: 6992 6992
Size: 8161
AhdI GACnn_n'nnGTC
Cuts at: 6890 6890
Size: 8161
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3870 1184
Size: 2686 5475
AlwNI CAG_nnn'CTG
Cuts at: 2627 4382 4445 5345 5904 7476 2627
Size: 1755 63 900 559 1572 3312
Fragments arranged by size:
3312 1755 1572 900 559 63
ApaLI G'TGCA_C
Cuts at: 4953 6218 7374 4953
Size: 1265 1156 5740
Fragments arranged by size:
5740 1265 1156
ApoI r'AATT_y
Cuts at: 1493 2309 5470 5696 5848 7957 1493
Size: 816 3161 226 152 2109 1697
Fragments arranged by size:
3161 2109 1697 816 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6715 160
Size: 872 5683 1606
Fragments arranged by size:
5683 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8161
AvaI C'yCGr_G
Cuts at: 1870 1943 5486 1870
Size: 73 3543 4545
Fragments arranged by size:
4545 3543 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2970 3003 2970
Size: 33 8128
BamHI G'GATC_C
Cuts at: 5219 5491 5858 5219
Size: 272 367 7522
Fragments arranged by size:
7522 367 272
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2755 5354 5482 6937 618
Size: 325 217 627 968 2599 128 1455 1842
Fragments arranged by size:
2599 1842 1455 968 627 325 217 128
BanII G_rGCy'C
Cuts at: 1391 4083 4481 5480 1391
Size: 2692 398 999 4072
Fragments arranged by size:
4072 2692 999 398
BbeI G_GCGC'C
Cuts at: 5358 5358
Size: 8161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2747 5270 928
Size: 971 848 2523 3819
Fragments arranged by size:
3819 2523 971 848
BbvCI CC'TCA_GC
Cuts at: 2643 2643
Size: 8161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6352 6386 1903
Size: 34 4415 34 3678
Fragments arranged by size:
4415 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6053 7263 6053
Size: 1210 6951
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8161
BfrBI ATG'CAT
Cuts at: 2030 2030
Size: 8161
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4693 5382 6772
Size: 107 122 71 631 605 3021 689 1390
Cuts at: 6772 136
Size: 1525
Fragments arranged by size:
3021 1525 1390 689 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4397 8156 4397
Size: 3759 4402
BlpI GC'TnA_GC
Cuts at: 4763 5578 4763
Size: 815 7346
Bme1580I G_kGCm'C
Cuts at: 1790 4957 6222 7378 1790
Size: 3167 1265 1156 2573
Fragments arranged by size:
3167 2573 1265 1156
BmgBI CAC'GTC
Cuts at: 1318 4315 1318
Size: 2997 5164
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3419 5892 6850
Size: 416 53 83 399 24 2002 2473 958
Cuts at: 6850 442
Size: 1753
Fragments arranged by size:
2473 2002 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2672 1056
Size: 1616 6545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3093 3171 3093
Size: 78 8083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 2455 3558 3600 3702 4378 4872 5079
Size: 792 424 1103 42 102 676 494 207
Cuts at: 5079 6821 1239
Size: 1742 2579
Fragments arranged by size:
2579 1742 1103 792 676 494 424 207
102 42
Bpu10I CC'TnA_GC
Cuts at: 2391 2643 3681 4179 2391
Size: 252 1038 498 6373
Fragments arranged by size:
6373 1038 498 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4597 6286 7151 7449 4597
Size: 1689 865 298 5309
Fragments arranged by size:
5309 1689 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2304 3989 5113 6824 882
Size: 617 805 1685 1124 1711 2219
Fragments arranged by size:
2219 1711 1685 1124 805 617
BsaAI yAC'GTr
Cuts at: 493 1204 5952 493
Size: 711 4748 2702
Fragments arranged by size:
4748 2702 711
BsaBI GATnn'nnATC
Cuts at: 8155 8155
Size: 8161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5355 5968 6350 6985
Size: 53 83 186 936 3822 613 382 635
Cuts at: 6985 275
Size: 1451
Fragments arranged by size:
3822 1451 936 635 613 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6594 6992 7266 7413 7824 6594
Size: 398 274 147 411 6931
Fragments arranged by size:
6931 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2112 2142 2235 2265 2284
Size: 30 165 30 709 30 93 30 19
Cuts at: 2284 2314 3556 3586 4774 4804 5148 5178 5244
Size: 30 1242 30 1188 30 344 30 66
Cuts at: 5244 5274 1178
Size: 30 4065
Fragments arranged by size:
4065 1242 1188 709 344 165 93 66
30 30 30 30 30 30 30 30
30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2631 3938 4869 5101 5172 5280 5333 1186
Size: 1445 1307 931 232 71 108 53 4014
Fragments arranged by size:
4014 1445 1307 931 232 108 71 53
BseYI C'CCAG_C
Cuts at: 3034 3220 3652 4387 4661 4742 5312 7364 7860
Size: 186 432 735 274 81 570 2052 496
Cuts at: 7860 3034
Size: 3335
Fragments arranged by size:
3335 2052 735 570 496 432 274 186
81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3840 3928 1163
Size: 573 2104 88 5396
Fragments arranged by size:
5396 2104 573 88
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3921 5611 6372 7400 1540
Size: 275 186 1920 1690 761 1028 2301
Fragments arranged by size:
2301 1920 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2207 4083 4415 4595 4957 6222 6307 7378 2207
Size: 1876 332 180 362 1265 85 1071 2990
Fragments arranged by size:
2990 1876 1265 1071 362 332 180 85
BsmI GAATG_Cn'
Cuts at: 5672 5765 5672
Size: 93 8068
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2207 4083 4415 4481 4595 4957 5480
Size: 399 417 1876 332 66 114 362 523
Cuts at: 5480 6222 6307 7378 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1876 1071 742 523 417 399 362
332 114 85 66
BspEI T'CCGG_A
Cuts at: 7824 7824
Size: 8161
BspHI T'CATG_A
Cuts at: 3867 6050 7780 3867
Size: 2183 1730 4248
Fragments arranged by size:
4248 2183 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4138 4540 4975 5254 5516 844
Size: 3294 402 435 279 262 3489
Fragments arranged by size:
3489 3294 435 402 279 262
BsrBI CCG'CTC
Cuts at: 1394 1823 4812 6048 1394
Size: 429 2989 1236 3507
Fragments arranged by size:
3507 2989 1236 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2079 4329 6656 6830 65
Size: 1183 831 2250 2327 174 1396
Fragments arranged by size:
2327 2250 1396 1183 831 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3916 4937 5323 6805 6992 1700
Size: 110 2106 1021 386 1482 187 2869
Fragments arranged by size:
2869 2106 1482 1021 386 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8161
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8161
BssSI C'ACGA_G
Cuts at: 6221 7233 6221
Size: 1012 7149
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5255 794
Size: 4461 3700
BstBI TT'CG_AA
Cuts at: 6965 6965
Size: 8161
BstEII G'GTnAC_C
Cuts at: 1184 5408 1184
Size: 4224 3937
BstXI CCAn_nnnn'nTGG
Cuts at: 2794 3004 3112 3190 3232 3478 2794
Size: 210 108 78 42 246 7477
Fragments arranged by size:
7477 246 210 108 78 42
BstYI r'GATC_y
Cuts at: 1972 4397 5219 5442 5491 5858 6243 6260 7712
Size: 2425 822 223 49 367 385 17 1452
Cuts at: 7712 7798 7810 8156 1972
Size: 86 12 346 1977
Fragments arranged by size:
2425 1977 1452 822 385 367 346 223
86 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 2565 4000 2565
Size: 1435 6726
BtgI C'CryG_G
Cuts at: 513 1065 2004 2889 4827 513
Size: 552 939 885 1938 3847
Fragments arranged by size:
3847 1938 939 885 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7422
Fragments arranged by size:
7422 683 56
BtsI GCAGTG_nn'
Cuts at: 3356 4506 5677 6471 6491 3356
Size: 1150 1171 794 20 5026
Fragments arranged by size:
5026 1171 1150 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8161
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4183 4218 5134 5169 529
Size: 35 3619 35 916 35 3521
Fragments arranged by size:
3619 3521 916 35 35 35
DraI TTT'AAA
Cuts at: 5465 5465
Size: 8161
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4558 7168 809
Size: 507 3242 2610 1802
Fragments arranged by size:
3242 2610 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2360 2868 3342
Size: 54 1425 50 275 18 530 508 474
Cuts at: 3342 3468 3702 3918 4116 4734 4773 5325 5383
Size: 126 234 216 198 618 39 552 58
Cuts at: 5383 5608 6497 8126 8
Size: 225 889 1629 43
Fragments arranged by size:
1629 1425 889 618 552 530 508 474
275 234 225 216 198 126 58 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 3918 5608 1537
Size: 275 2106 1690 4090
Fragments arranged by size:
4090 2106 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 2691 6091 1353
Size: 960 378 3400 3423
Fragments arranged by size:
3423 3400 960 378
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6748 7122 7268 387
Size: 1064 138 5159 374 146 1280
Fragments arranged by size:
5159 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4081 4081
Size: 8161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2455 2759 2976 3558 3600 3702
Size: 393 399 424 304 217 582 42 102
Cuts at: 3702 3975 4221 4378 4820 4872 5079 5349 5367
Size: 273 246 157 442 52 207 270 18
Cuts at: 5367 6224 6821 7608 7876 1239
Size: 857 597 787 268 1524
Fragments arranged by size:
1524 857 787 597 582 442 424 399
393 304 273 270 268 246 217 207
157 102 52 42 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2786 2912 2954 3638 3680 4285 4854 5154 5250
Size: 126 42 684 42 605 569 300 96
Cuts at: 5250 2786
Size: 5697
Fragments arranged by size:
5697 684 605 569 300 126 96 42
42
EcoO109I rG'GnC_Cy
Cuts at: 3649 5303 3649
Size: 1654 6507
EcoRI G'AATT_C
Cuts at: 2309 5470 5848 2309
Size: 3161 378 4622
Fragments arranged by size:
4622 3161 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 3928 5206 2092
Size: 1836 1278 5047
Fragments arranged by size:
5047 1836 1278
FspI TGC'GCA
Cuts at: 6667 6667
Size: 8161
HaeII r_GCGC'y
Cuts at: 1678 5345 5358 7308 1678
Size: 3667 13 1950 2531
Fragments arranged by size:
3667 2531 1950 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 2825 2857 3059
Size: 32 135 32 647 32 574 32 202
Cuts at: 3059 3091 3137 3169 3263 3295 3556 3588 3628
Size: 32 46 32 94 32 261 32 40
Cuts at: 3628 3660 5148 5161 5180 5193 6802 6834 6876
Size: 32 1488 13 19 13 1609 32 42
Cuts at: 6876 6908 1373
Size: 32 2626
Fragments arranged by size:
2626 1609 1488 647 574 261 202 135
94 46 42 40 32 32 32 32
32 32 32 32 32 32 32 19
13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3974 5505 5751 669
Size: 988 281 2036 1531 246 3079
Fragments arranged by size:
3079 2036 1531 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8161
HpaI GTT'AAC
Cuts at: 5751 5751
Size: 8161
KasI G'GCGC_C
Cuts at: 5354 5354
Size: 8161
KpnI G_GTAC'C
Cuts at: 5486 5486
Size: 8161
MfeI C'AATT_G
Cuts at: 4944 5760 4944
Size: 816 7345
MluI A'CGCG_T
Cuts at: 8037 8037
Size: 8161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2110 2327 2410 7275 7459 1305
Size: 805 217 83 4865 184 2007
Fragments arranged by size:
4865 2007 805 217 184 83
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2362 2870 3344 3704 4118
Size: 54 1425 343 530 508 474 360 414
Cuts at: 4118 4775 5385 10
Size: 657 610 2786
Fragments arranged by size:
2786 1425 657 610 530 508 474 414
360 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2442 4682 6119 6478 6637 518
Size: 648 1276 2240 1437 359 159 2042
Fragments arranged by size:
2240 2042 1437 1276 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2483 2555 3854 4253 4442 4571 4646 4892
Size: 877 72 1299 399 189 129 75 246
Cuts at: 4892 5577 6254 7402 7647 1606
Size: 685 677 1148 245 2120
Fragments arranged by size:
2120 1299 1148 877 685 677 399 246
245 189 129 75 72
NaeI GCC'GGC
Cuts at: 3918 4939 5325 3918
Size: 1021 386 6754
Fragments arranged by size:
6754 1021 386
NarI GG'CG_CC
Cuts at: 5355 5355
Size: 8161
NcoI C'CATG_G
Cuts at: 513 1065 2004 2889 513
Size: 552 939 885 5785
Fragments arranged by size:
5785 939 885 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8161
NgoMIV G'CCGG_C
Cuts at: 3916 4937 5323 3916
Size: 1021 386 6754
Fragments arranged by size:
6754 1021 386
NheI G'CTAG_C
Cuts at: 1052 2668 1052
Size: 1616 6545
NotI GC'GGCC_GC
Cuts at: 5608 5608
Size: 8161
NsiI A_TGCA'T
Cuts at: 2032 2032
Size: 8161
NspI r_CATG'y
Cuts at: 1132 1229 2881 3523 5054 5519 7064 1132
Size: 97 1652 642 1531 465 1545 2229
Fragments arranged by size:
2229 1652 1545 1531 642 465 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8161
PciI A'CATG_T
Cuts at: 3519 7060 3519
Size: 3541 4620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5431 1108
Size: 4323 3838
PfoI T'CCnGG_A
Cuts at: 2154 5445 2154
Size: 3291 4870
PmeI GTTT'AAAC
Cuts at: 5465 5465
Size: 8161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6230 6262 7743 7775 6230
Size: 32 1481 32 6616
Fragments arranged by size:
6616 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3649 5303 3649
Size: 1654 6507
PshAI GACnn'nnGTC
Cuts at: 1613 2016 4578 1613
Size: 403 2562 5196
Fragments arranged by size:
5196 2562 403
PsiI TTA'TAA
Cuts at: 5731 5731
Size: 8161
PstI C_TGCA'G
Cuts at: 830 3837 4537 4651 5251 5513 830
Size: 3007 700 114 600 262 3478
Fragments arranged by size:
3478 3007 700 600 262 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8161
PvuII CAG'CTG
Cuts at: 2483 2555 3854 4253 4442 4571 4892 2483
Size: 72 1299 399 189 129 321 5752
Fragments arranged by size:
5752 1299 399 321 189 129 72
SacI G_AGCT'C
Cuts at: 4083 4083
Size: 8161
SalI G'TCGA_C
Cuts at: 1936 5503 1936
Size: 3567 4594
SanDI GG'GwC_CC
Cuts at: 5303 5303
Size: 8161
SbfI CC_TGCA'GG
Cuts at: 5251 5513 5251
Size: 262 7899
ScaI AGT'ACT
Cuts at: 6409 8121 6409
Size: 1712 6449
SfcI C'TryA_G
Cuts at: 826 1045 2292 3833 4533 4647 5005 5247 5509
Size: 219 1247 1541 700 114 358 242 262
Cuts at: 5509 6644 7325 7516 826
Size: 1135 681 191 1471
Fragments arranged by size:
1541 1471 1247 1135 700 681 358 262
242 219 191 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 5382 5382
Size: 8161
SfoI GGC'GCC
Cuts at: 5356 5356
Size: 8161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8161
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8161
SmaI CCC'GGG
Cuts at: 1872 5488 1872
Size: 3616 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4576 6265 7166 7428 820
Size: 197 926 2633 1689 901 262 1553
Fragments arranged by size:
2633 1689 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5952 493
Size: 5459 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8161
SphI G_CATG'C
Cuts at: 1132 5519 1132
Size: 4387 3774
SspI AAT'ATT
Cuts at: 5 52 2496 5044 6085 5
Size: 47 2444 2548 1041 2081
Fragments arranged by size:
2548 2444 2081 1041 47
StuI AGG'CCT
Cuts at: 1517 2102 4030 4291 1517
Size: 585 1928 261 5387
Fragments arranged by size:
5387 1928 585 261
StyI C'CwwG_G
Cuts at: 513 1065 2004 2673 2787 2871 2889 3039 3105
Size: 552 939 669 114 84 18 150 66
Cuts at: 3105 3183 3225 3387 3471 4815 5034 5211 5430
Size: 78 42 162 84 1344 219 177 219
Cuts at: 5430 5600 513
Size: 170 3074
Fragments arranged by size:
3074 1344 939 669 552 219 219 177
170 162 150 114 84 84 78 66
42 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2860 3136 3214 4276 4696 6188 6205
Size: 1255 1280 276 78 1062 420 1492 17
Cuts at: 6205 6358 6543 6988 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1492 1280 1255 1062 445 420 276
185 153 78 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6407 8119 96
Size: 274 80 33 51 1443 4430 1712 138
Fragments arranged by size:
4430 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2591 2623 4017 4049 5244 5276 8121 8153 2591
Size: 32 1394 32 1195 32 2845 32 2599
Fragments arranged by size:
2845 2599 1394 1195 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8161
XbaI T'CTAG_A
Cuts at: 5497 5497
Size: 8161
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8161
XmaI C'CCGG_G
Cuts at: 1870 5486 1870
Size: 3616 4545
XmnI GAAnn'nnTTC
Cuts at: 3588 6290 3588
Size: 2702 5459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5969 276
Size: 53 83 186 936 4435 2468
Fragments arranged by size:
4435 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BsiWI BsmBI
BstZ17I DraIII EcoRV FseI FspAI NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SapI SexAI SrfI
SwaI XcmI