Restriction map for
pFN21AE2564
(Circular) (Six-base) MAPSORT of: pFN21AE2564.seq Check: 7048 from: 1 to: 6529
pFN21AE2564 6529 bp
With 182 enzymes: SgfI *
March 13, 2015 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3107 3107
Size: 6529
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4339 278
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3872 1937
Size: 1935 4594
Acc65I G'GTAC_C
Cuts at: 3850 3850
Size: 6529
AclI AA'CG_TT
Cuts at: 1563 4656 5029 1563
Size: 3093 373 3063
Fragments arranged by size:
3093 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2558 3328 3751 4592 5976 6244 1632
Size: 926 770 423 841 1384 268 1917
Fragments arranged by size:
1917 1384 926 841 770 423 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6332
AflIII A'CryG_T
Cuts at: 1203 5428 6405 1203
Size: 4225 977 1327
Fragments arranged by size:
4225 1327 977
AgeI A'CCGG_T
Cuts at: 5360 5360
Size: 6529
AhdI GACnn_n'nnGTC
Cuts at: 5258 5258
Size: 6529
AleI CACnn'nnGTG
Cuts at: 1166 2682 3452 3617 3756 1166
Size: 1516 770 165 139 3939
Fragments arranged by size:
3939 1516 770 165 139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6529
AlwNI CAG_nnn'CTG
Cuts at: 2022 2403 3053 4272 5844 2022
Size: 381 650 1219 1572 2707
Fragments arranged by size:
2707 1572 1219 650 381
ApaI G_GGCC'C
Cuts at: 2102 2886 2102
Size: 784 5745
ApaLI G'TGCA_C
Cuts at: 3740 4586 5742 3740
Size: 846 1156 4527
Fragments arranged by size:
4527 1156 846
ApoI r'AATT_y
Cuts at: 1493 3838 4064 4216 6325 1493
Size: 2345 226 152 2109 1697
Fragments arranged by size:
2345 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5083 160
Size: 872 4051 1606
Fragments arranged by size:
4051 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
AvaI C'yCGr_G
Cuts at: 1870 1943 2382 2628 3854 1870
Size: 73 439 246 1226 4545
Fragments arranged by size:
4545 1226 439 246 73
BamHI G'GATC_C
Cuts at: 2817 3859 4226 2817
Size: 1042 367 5120
Fragments arranged by size:
5120 1042 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 2504 3086 3360 3446
Size: 325 217 627 417 300 582 274 86
Cuts at: 3446 3850 5305 618
Size: 404 1455 1842
Fragments arranged by size:
1842 1455 627 582 417 404 325 300
274 217 86
BanII G_rGCy'C
Cuts at: 1391 2036 2102 2712 2886 3848 1391
Size: 645 66 610 174 962 4072
Fragments arranged by size:
4072 962 645 610 174 66
BbeI G_GCGC'C
Cuts at: 2508 3364 2508
Size: 856 5673
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3210 3495 928
Size: 971 1311 285 3962
Fragments arranged by size:
3962 1311 971 285
BbvCI CC'TCA_GC
Cuts at: 2748 3459 2748
Size: 711 5818
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2402 2436 4720 4754 1903
Size: 34 50 34 381 34 2284 34 3678
Fragments arranged by size:
3678 2284 381 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4421 5631 4421
Size: 1210 5319
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6529
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2454 2939 5140
Size: 107 122 71 631 605 782 485 2201
Cuts at: 5140 136
Size: 1525
Fragments arranged by size:
2201 1525 782 631 605 485 122 107
71
BglII A'GATC_T
Cuts at: 3157 6524 3157
Size: 3367 3162
BlpI GC'TnA_GC
Cuts at: 3259 3268 3946 3259
Size: 9 678 5842
Fragments arranged by size:
5842 678 9
Bme1580I G_kGCm'C
Cuts at: 1790 2102 2207 2558 2729 2886 3079 3744 4590
Size: 312 105 351 171 157 193 665 846
Cuts at: 4590 5746 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 846 665 351 312 193 171
157 105
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2811 2926 2963
Size: 416 53 83 399 24 1394 115 37
Cuts at: 2963 3007 4260 5218 442
Size: 44 1253 958 1753
Fragments arranged by size:
1753 1394 1253 958 416 399 115 83
53 44 37 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6529
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2664 3529 2664
Size: 865 5664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2535 2948 3150 3626 5189 1239
Size: 1296 413 202 476 1563 2579
Fragments arranged by size:
2579 1563 1296 476 413 202
Bpu10I CC'TnA_GC
Cuts at: 2748 3459 2748
Size: 711 5818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2359 4654 5519 5817 2359
Size: 2295 865 298 3071
Fragments arranged by size:
3071 2295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2085 2804 2981 3182 5192 882
Size: 617 586 719 177 201 2010 2219
Fragments arranged by size:
2219 2010 719 617 586 201 177
BsaAI yAC'GTr
Cuts at: 493 1204 4320 493
Size: 711 3116 2702
Fragments arranged by size:
3116 2702 711
BsaBI GATnn'nnATC
Cuts at: 3608 6523 3608
Size: 2915 3614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2505 3295 3361 4336
Size: 53 83 186 936 972 790 66 975
Cuts at: 4336 4718 5353 275
Size: 382 635 1451
Fragments arranged by size:
1451 975 972 936 790 635 382 186
83 66 53
BsaWI w'CCGG_w
Cuts at: 2781 4962 5360 5634 5781 6192 2781
Size: 2181 398 274 147 411 3118
Fragments arranged by size:
3118 2181 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2978 3008 1178
Size: 30 165 30 1575 30 4699
Fragments arranged by size:
4699 1575 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3301 1186
Size: 2115 4414
BseYI C'CCAG_C
Cuts at: 2260 2388 2423 2611 2892 3356 3567 5732 6228
Size: 128 35 188 281 464 211 2165 496
Cuts at: 6228 2260
Size: 2561
Fragments arranged by size:
2561 2165 496 464 281 211 188 128
35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2083 3028 3152 3203 1163
Size: 573 347 945 124 51 4489
Fragments arranged by size:
4489 945 573 347 124 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3979 4740 5768 1540
Size: 275 186 1978 761 1028 2301
Fragments arranged by size:
2301 1978 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2629 2712 3744 4590 4675 5746 2629
Size: 83 1032 846 85 1071 3412
Fragments arranged by size:
3412 1071 1032 846 85 83
BsmI GAATG_Cn'
Cuts at: 4040 4133 4040
Size: 93 6436
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2102 2207 2558 2629 2712 2729
Size: 399 246 66 105 351 71 83 17
Cuts at: 2729 2886 3079 3744 3848 4590 4675 5746 1391
Size: 157 193 665 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 665 399 351 246 193
157 105 104 85 83 71 66 17
BspEI T'CCGG_A
Cuts at: 6192 6192
Size: 6529
BspHI T'CATG_A
Cuts at: 4418 6148 4418
Size: 1730 4799
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2589 3107 3884 844
Size: 1745 518 777 3489
Fragments arranged by size:
3489 1745 777 518
BsrBI CCG'CTC
Cuts at: 1394 1823 3267 4416 1394
Size: 429 1444 1149 3507
Fragments arranged by size:
3507 1444 1149 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3705 5024 5198 65
Size: 1183 2457 1319 174 1396
Fragments arranged by size:
2457 1396 1319 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3207 3291 3579 5173 5360 1700
Size: 110 1397 84 288 1594 187 2869
Fragments arranged by size:
2869 1594 1397 288 187 110 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6529
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6529
BssSI C'ACGA_G
Cuts at: 3380 4589 5601 3380
Size: 1209 1012 4308
Fragments arranged by size:
4308 1209 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2070 2211 794
Size: 1276 141 5112
Fragments arranged by size:
5112 1276 141
BstBI TT'CG_AA
Cuts at: 5333 5333
Size: 6529
BstEII G'GTnAC_C
Cuts at: 1184 3287 1184
Size: 2103 4426
BstYI r'GATC_y
Cuts at: 1972 2817 3157 3318 3571 3859 4226 4611 4628
Size: 845 340 161 253 288 367 385 17
Cuts at: 4628 6080 6166 6178 6524 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 845 385 367 346 340 288
253 161 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2094 2934 2974 3388 513
Size: 552 1029 840 40 414 3654
Fragments arranged by size:
3654 1029 840 552 414 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2229 487
Size: 683 56 1003 4787
Fragments arranged by size:
4787 1003 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 2353 3123 4045 4839 4859 2033
Size: 320 770 922 794 20 3703
Fragments arranged by size:
3703 922 794 770 320 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6494
DraI TTT'AAA
Cuts at: 3833 3833
Size: 6529
DraIII CAC_nnn'GTG
Cuts at: 3498 3498
Size: 6529
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2471 3058 5536 809
Size: 507 1155 587 2478 1802
Fragments arranged by size:
2478 1802 1155 587 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3252 3805 3976
Size: 54 1425 50 275 18 1422 553 171
Cuts at: 3976 4865 6494 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1422 889 553 275 171 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3976 1537
Size: 275 2164 4090
Fragments arranged by size:
4090 2164 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3234 3768 4459 1353
Size: 1881 534 691 3423
Fragments arranged by size:
3423 1881 691 534
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5116 5490 5636 387
Size: 1064 138 3527 374 146 1280
Fragments arranged by size:
3527 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2710 2710
Size: 6529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2535 2558 2948 3150 3328 3626 3751
Size: 393 903 23 390 202 178 298 125
Cuts at: 3751 4592 5189 5976 6244 1239
Size: 841 597 787 268 1524
Fragments arranged by size:
1524 903 841 787 597 393 390 298
268 202 178 125 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2313 3103 2313
Size: 790 5739
EcoO109I rG'GnC_Cy
Cuts at: 2099 2578 2876 2883 2896 2922 3114 2099
Size: 479 298 7 13 26 192 5514
Fragments arranged by size:
5514 479 298 192 26 13 7
EcoRI G'AATT_C
Cuts at: 3838 4216 3838
Size: 378 6151
EcoRV GAT'ATC
Cuts at: 3430 3430
Size: 6529
FspI TGC'GCA
Cuts at: 5035 5035
Size: 6529
HaeII r_GCGC'y
Cuts at: 1678 2449 2508 2649 3364 5676 1678
Size: 771 59 141 715 2312 2531
Fragments arranged by size:
2531 2312 771 715 141 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2694 2726 3137 3169 3717
Size: 32 135 32 1122 32 411 32 548
Cuts at: 3717 3749 5170 5202 5244 5276 1373
Size: 32 1421 32 42 32 2626
Fragments arranged by size:
2626 1421 1122 548 411 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3873 4119 669
Size: 988 281 1935 246 3079
Fragments arranged by size:
3079 1935 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6529
HpaI GTT'AAC
Cuts at: 4119 4119
Size: 6529
KasI G'GCGC_C
Cuts at: 2504 3360 2504
Size: 856 5673
KpnI G_GTAC'C
Cuts at: 3854 3854
Size: 6529
MfeI C'AATT_G
Cuts at: 4128 4128
Size: 6529
MluI A'CGCG_T
Cuts at: 6405 6405
Size: 6529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2845 5643 5827 1305
Size: 1540 2798 184 2007
Fragments arranged by size:
2798 2007 1540 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3254 3807 10
Size: 54 1425 343 1422 553 2732
Fragments arranged by size:
2732 1425 1422 553 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2682 3452 3557 3617 3756 4487 4846
Size: 648 1516 770 105 60 139 731 359
Cuts at: 4846 5005 518
Size: 159 2042
Fragments arranged by size:
2042 1516 770 731 648 359 159 139
105 60
MspA1I CmG'CkG
Cuts at: 1606 2747 3945 4622 5770 6015 1606
Size: 1141 1198 677 1148 245 2120
Fragments arranged by size:
2120 1198 1148 1141 677 245
NarI GG'CG_CC
Cuts at: 2505 3361 2505
Size: 856 5673
NcoI C'CATG_G
Cuts at: 513 1065 2934 3388 513
Size: 552 1869 454 3654
Fragments arranged by size:
3654 1869 552 454
NdeI CA'TA_TG
Cuts at: 387 3219 387
Size: 2832 3697
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6529
NotI GC'GGCC_GC
Cuts at: 3976 3976
Size: 6529
NspI r_CATG'y
Cuts at: 1132 1229 2588 3887 5432 1132
Size: 97 1359 1299 1545 2229
Fragments arranged by size:
2229 1545 1359 1299 97
PasI CC'CwG_GG
Cuts at: 1348 2037 1348
Size: 689 5840
PciI A'CATG_T
Cuts at: 5428 5428
Size: 6529
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2283 2550 3328 3406 1108
Size: 1175 267 778 78 4231
Fragments arranged by size:
4231 1175 778 267 78
PmeI GTTT'AAAC
Cuts at: 3833 3833
Size: 6529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4598 4630 6111 6143 4598
Size: 32 1481 32 4984
Fragments arranged by size:
4984 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 2876 2922 3114 2578
Size: 298 46 192 5993
Fragments arranged by size:
5993 298 192 46
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6529
PsiI TTA'TAA
Cuts at: 4099 4099
Size: 6529
PspOMI G'GGCC_C
Cuts at: 2098 2882 2098
Size: 784 5745
PstI C_TGCA'G
Cuts at: 830 2235 2348 2985 3881 830
Size: 1405 113 637 896 3478
Fragments arranged by size:
3478 1405 896 637 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6529
PvuII CAG'CTG
Cuts at: 2747 2747
Size: 6529
SacI G_AGCT'C
Cuts at: 2712 2712
Size: 6529
SalI G'TCGA_C
Cuts at: 1936 3871 1936
Size: 1935 4594
SanDI GG'GwC_CC
Cuts at: 2922 3114 2922
Size: 192 6337
SbfI CC_TGCA'GG
Cuts at: 2985 3881 2985
Size: 896 5633
ScaI AGT'ACT
Cuts at: 4777 6489 4777
Size: 1712 4817
SexAI A'CCwGG_T
Cuts at: 2990 2990
Size: 6529
SfcI C'TryA_G
Cuts at: 826 1045 2231 2344 2944 2981 3877 5012 5693
Size: 219 1186 113 600 37 896 1135 681
Cuts at: 5693 5884 826
Size: 191 1471
Fragments arranged by size:
1471 1186 1135 896 681 600 219 191
113 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 2939 2939
Size: 6529
SfoI GGC'GCC
Cuts at: 2506 3362 2506
Size: 856 5673
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
SgrAI Cr'CCGG_yG
Cuts at: 1700 3291 1700
Size: 1591 4938
SmaI CCC'GGG
Cuts at: 1872 2384 3856 1872
Size: 512 1472 4545
Fragments arranged by size:
4545 1472 512
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 2628 4633 5534 5796 820
Size: 197 926 395 290 2005 901 262 1553
Fragments arranged by size:
2005 1553 926 901 395 290 262 197
SnaBI TAC'GTA
Cuts at: 493 4320 493
Size: 3827 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6529
SphI G_CATG'C
Cuts at: 1132 2588 3887 1132
Size: 1456 1299 3774
Fragments arranged by size:
3774 1456 1299
SspI AAT'ATT
Cuts at: 5 52 4453 5
Size: 47 4401 2081
Fragments arranged by size:
4401 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6529
StyI C'CwwG_G
Cuts at: 513 1065 2934 3351 3388 3450 3968 513
Size: 552 1869 417 37 62 518 3074
Fragments arranged by size:
3074 1869 552 518 417 62 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 2657 3104 3361 3727 4556 4573
Size: 1255 789 288 447 257 366 829 17
Cuts at: 4573 4726 4911 5356 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 829 789 447 445 366 288
257 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3225 4775 6487
Size: 274 80 33 51 1443 1248 1550 1712
Cuts at: 6487 96
Size: 138
Fragments arranged by size:
1712 1550 1443 1248 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2278 2310 6489 6521 2278
Size: 32 4179 32 2286
Fragments arranged by size:
4179 2286 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6529
XbaI T'CTAG_A
Cuts at: 3865 3865
Size: 6529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2450 2450
Size: 6529
XhoI C'TCGA_G
Cuts at: 1943 2628 1943
Size: 685 5844
XmaI C'CCGG_G
Cuts at: 1870 2382 3854 1870
Size: 512 1472 4545
Fragments arranged by size:
4545 1472 512
XmnI GAAnn'nnTTC
Cuts at: 4658 4658
Size: 6529
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4337 276
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SapI SrfI SwaI