Restriction map for
pFN21AE2561
(Circular) (Six-base) MAPSORT of: pFN21AE2561.seq Check: 6382 from: 1 to: 12358
pFN21AE2561 12358 bp
With 182 enzymes: SgfI *
March 4, 2015 12:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4694 8906 4694
Size: 4212 8146
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5401 5598 10168 278
Size: 53 83 186 936 3865 197 4570 2468
Fragments arranged by size:
4570 3865 2468 936 197 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2879 4113 8568 9701 1937
Size: 942 1234 4455 1133 4594
Fragments arranged by size:
4594 4455 1234 1133 942
Acc65I G'GTAC_C
Cuts at: 6984 9679 6984
Size: 2695 9663
AclI AA'CG_TT
Cuts at: 1563 10485 10858 1563
Size: 8922 373 3063
Fragments arranged by size:
8922 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2406 2667 4157 5654 6121 7445 9636 10421
Size: 774 261 1490 1497 467 1324 2191 785
Cuts at: 10421 11805 12073 1632
Size: 1384 268 1917
Fragments arranged by size:
2191 1917 1497 1490 1384 1324 785 774
467 268 261
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12161
AflIII A'CryG_T
Cuts at: 1203 3083 3360 5247 8199 8201 11257 12234 1203
Size: 1880 277 1887 2952 2 3056 977 1327
Fragments arranged by size:
3056 2952 1887 1880 1327 977 277 2
AgeI A'CCGG_T
Cuts at: 11189 11189
Size: 12358
AhdI GACnn_n'nnGTC
Cuts at: 11087 11087
Size: 12358
AleI CACnn'nnGTG
Cuts at: 1166 4034 4107 4688 6113 8249 8454 1166
Size: 2868 73 581 1425 2136 205 5070
Fragments arranged by size:
5070 2868 2136 1425 581 205 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2519 4783 7607 8565 9209 1184
Size: 1335 2264 2824 958 644 4333
Fragments arranged by size:
4333 2824 2264 1335 958 644
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7382 7414 7382
Size: 32 12326
AlwNI CAG_nnn'CTG
Cuts at: 3883 5233 7854 8956 10101 11673 3883
Size: 1350 2621 1102 1145 1572 4568
Fragments arranged by size:
4568 2621 1572 1350 1145 1102
ApaI G_GGCC'C
Cuts at: 2324 4500 2324
Size: 2176 10182
ApaLI G'TGCA_C
Cuts at: 6496 6592 10415 11571 6496
Size: 96 3823 1156 7283
Fragments arranged by size:
7283 3823 1156 96
ApoI r'AATT_y
Cuts at: 1493 2053 2623 2941 3936 5802 6707 7186 7221
Size: 560 570 318 995 1866 905 479 35
Cuts at: 7221 7268 9667 9893 10045 12154 1493
Size: 47 2399 226 152 2109 1697
Fragments arranged by size:
2399 2109 1866 1697 995 905 570 560
479 318 226 152 47 35
AseI AT'TA_AT
Cuts at: 160 1032 9507 10912 160
Size: 872 8475 1405 1606
Fragments arranged by size:
8475 1606 1405 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12358
AvaI C'yCGr_G
Cuts at: 1870 1943 4802 6936 7699 8440 9021 9683 1870
Size: 73 2859 2134 763 741 581 662 4545
Fragments arranged by size:
4545 2859 2134 763 741 662 581 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 8577 8610 8577
Size: 33 12325
BamHI G'GATC_C
Cuts at: 9688 10055 9688
Size: 367 11991
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4676 5970 6984 9068 9185
Size: 325 217 627 2889 1294 1014 2084 117
Cuts at: 9185 9679 11134 618
Size: 494 1455 1842
Fragments arranged by size:
2889 2084 1842 1455 1294 1014 627 494
325 217 117
BanII G_rGCy'C
Cuts at: 1391 2324 4086 4500 7605 8084 9677 1391
Size: 933 1762 414 3105 479 1593 4072
Fragments arranged by size:
4072 3105 1762 1593 933 479 414
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5229 5956 6309 7508 8857 928
Size: 971 3330 727 353 1199 1349 4429
Fragments arranged by size:
4429 3330 1349 1199 971 727 353
BbvCI CC'TCA_GC
Cuts at: 4486 9574 4486
Size: 5088 7270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2721 2755 3007 3027 3041 3061 7534
Size: 34 784 34 252 20 14 20 4473
Cuts at: 7534 7568 10549 10583 1903
Size: 34 2981 34 3678
Fragments arranged by size:
4473 3678 2981 784 252 34 34 34
34 20 20 14
BciVI GTATCCnnnnn_n'
Cuts at: 8336 8578 10250 11460 8336
Size: 242 1672 1210 9234
Fragments arranged by size:
9234 1672 1210 242
BclI T'GATC_A
Cuts at: 1548 7936 1548
Size: 6388 5970
BfrBI ATG'CAT
Cuts at: 3048 4296 4659 5659 5925 3048
Size: 1248 363 1000 266 9481
Fragments arranged by size:
9481 1248 1000 363 266
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2319 2802 4281
Size: 107 122 71 631 605 647 483 1479
Cuts at: 4281 4437 8657 8717 10969 136
Size: 156 4220 60 2252 1525
Fragments arranged by size:
4220 2252 1525 1479 647 631 605 483
156 122 107 71 60
BglII A'GATC_T
Cuts at: 3599 5221 6871 12353 3599
Size: 1622 1650 5482 3604
Fragments arranged by size:
5482 3604 1650 1622
BlpI GC'TnA_GC
Cuts at: 8737 9016 9775 8737
Size: 279 759 11320
Fragments arranged by size:
11320 759 279
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2324 2342 4031 4153 4500 4679 6500
Size: 378 156 18 1689 122 347 179 1821
Cuts at: 6500 6596 8315 8361 10419 11575 1790
Size: 96 1719 46 2058 1156 2573
Fragments arranged by size:
2573 2058 1821 1719 1689 1156 378 347
179 156 122 96 46 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4034 4318 4749
Size: 416 53 83 399 24 2617 284 431
Cuts at: 4749 6738 6829 6958 7325 8630 9429 10089 11047
Size: 1989 91 129 367 1305 799 660 958
Cuts at: 11047 442
Size: 1753
Fragments arranged by size:
2617 1989 1753 1305 958 799 660 431
416 399 367 284 129 91 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12358
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 4062 9562 2572
Size: 1490 5500 5368
Fragments arranged by size:
5500 5368 1490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2973 3717 4078 4614 4957 5639 6666 7914
Size: 1734 744 361 536 343 682 1027 1248
Cuts at: 7914 8771 11018 1239
Size: 857 2247 2579
Fragments arranged by size:
2579 2247 1734 1248 1027 857 744 682
536 361 343
Bpu10I CC'TnA_GC
Cuts at: 4249 4486 5693 9574 4249
Size: 237 1207 3881 7033
Fragments arranged by size:
7033 3881 1207 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 9650 9667 10483 11348 11646 9650
Size: 17 816 865 298 10362
Fragments arranged by size:
10362 865 816 298 17
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5660 6149 8326 8747 9104 9251 11021
Size: 617 4161 489 2177 421 357 147 1770
Cuts at: 11021 882
Size: 2219
Fragments arranged by size:
4161 2219 2177 1770 617 489 421 357
147
BsaAI yAC'GTr
Cuts at: 493 1204 2763 3067 6501 8202 10149 493
Size: 711 1559 304 3434 1701 1947 2702
Fragments arranged by size:
3434 2702 1947 1701 1559 711 304
BsaBI GATnn'nnATC
Cuts at: 3335 5366 7841 12352 3335
Size: 2031 2475 4511 3341
Fragments arranged by size:
4511 3341 2475 2031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5398 5595 8556 10165
Size: 53 83 186 936 3865 197 2961 1609
Cuts at: 10165 10547 11182 275
Size: 382 635 1451
Fragments arranged by size:
3865 2961 1609 1451 936 635 382 197
186 83 53
BsaWI w'CCGG_w
Cuts at: 5601 7952 9462 10791 11189 11463 11610 12021 5601
Size: 2351 1510 1329 398 274 147 411 5938
Fragments arranged by size:
5938 2351 1510 1329 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4051 4081 4456 4486 7592
Size: 30 165 30 2648 30 375 30 3106
Cuts at: 7592 7622 8687 8717 9248 9278 9562 9592 1178
Size: 30 1065 30 531 30 284 30 3944
Fragments arranged by size:
3944 3106 2648 1065 531 375 284 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4319 5603 5636 5857 5932 6288 1186
Size: 3133 1284 33 221 75 356 7256
Fragments arranged by size:
7256 3133 1284 356 221 75 33
BseYI C'CCAG_C
Cuts at: 2065 2316 2477 3208 3789 5674 7730 9097 9205
Size: 251 161 731 581 1885 2056 1367 108
Cuts at: 9205 11561 12057 2065
Size: 2356 496 2366
Fragments arranged by size:
2366 2356 2056 1885 1367 731 581 496
251 161 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2622 2983 3482 4287 4526 4779 5630
Size: 573 886 361 499 805 239 253 851
Cuts at: 5630 6145 6394 6399 6646 7613 7846 8350 8406
Size: 515 249 5 247 967 233 504 56
Cuts at: 8406 8878 1163
Size: 472 4643
Fragments arranged by size:
4643 967 886 851 805 573 515 504
499 472 361 253 249 247 239 233
56 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9808 10569 11597 1540
Size: 275 186 7807 761 1028 2301
Fragments arranged by size:
7807 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6500 6596 7091 7605 8418 8738 9138 9569 10419
Size: 96 495 514 813 320 400 431 850
Cuts at: 10419 10504 11575 6500
Size: 85 1071 7283
Fragments arranged by size:
7283 1071 850 813 514 495 431 400
320 96 85
BsmI GAATG_Cn'
Cuts at: 2672 3013 3050 4661 5500 6014 6061 6589 9869
Size: 341 37 1611 839 514 47 528 3280
Cuts at: 9869 9962 2672
Size: 93 5068
Fragments arranged by size:
5068 3280 1611 839 528 514 341 93
47 37
BsmBI CGTCTCn'nnnn_
Cuts at: 4443 7084 4443
Size: 2641 9717
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2324 2342 4031 4086 4153 4500
Size: 399 378 156 18 1689 55 67 347
Cuts at: 4500 4679 6500 6596 7091 7605 8084 8315 8361
Size: 179 1821 96 495 514 479 231 46
Cuts at: 8361 8418 8738 9138 9569 9677 10419 10504 11575
Size: 57 320 400 431 108 742 85 1071
Cuts at: 11575 1391
Size: 2174
Fragments arranged by size:
2174 1821 1689 1071 742 514 495 479
431 400 399 378 347 320 231 179
156 108 96 85 67 57 55 46
18
BspEI T'CCGG_A
Cuts at: 12021 12021
Size: 12358
BspHI T'CATG_A
Cuts at: 10247 11977 10247
Size: 1730 10628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 2417 4113 4694 5430 5652 5888 6167
Size: 1364 209 1696 581 736 222 236 279
Cuts at: 6167 6794 7384 7589 7794 8906 8941 8952 9713
Size: 627 590 205 205 1112 35 11 761
Cuts at: 9713 844
Size: 3489
Fragments arranged by size:
3489 1696 1364 1112 761 736 627 590
581 279 236 222 209 205 205 35
11
BsrBI CCG'CTC
Cuts at: 1394 1823 2351 4143 7822 10245 1394
Size: 429 528 1792 3679 2423 3507
Fragments arranged by size:
3679 3507 2423 1792 528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2371 3477 3906 4168 4549 4654 6319
Size: 1183 1123 1106 429 262 381 105 1665
Cuts at: 6319 6445 9643 10853 11027 65
Size: 126 3198 1210 174 1396
Fragments arranged by size:
3198 1665 1396 1210 1183 1123 1106 429
381 262 174 126 105
BsrFI r'CCGG_y
Cuts at: 1700 1810 6017 7973 11002 11189 1700
Size: 110 4207 1956 3029 187 2869
Fragments arranged by size:
4207 3029 2869 1956 187 110
BsrGI T'GTAC_A
Cuts at: 96 2047 2789 96
Size: 1951 742 9665
Fragments arranged by size:
9665 1951 742
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12358
BssSI C'ACGA_G
Cuts at: 5099 8122 8411 10418 11430 5099
Size: 3023 289 2007 1012 6027
Fragments arranged by size:
6027 3023 2007 1012 289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2706 6150 6422 6534 9353 794
Size: 1912 3444 272 112 2819 3799
Fragments arranged by size:
3799 3444 2819 1912 272 112
BstBI TT'CG_AA
Cuts at: 11162 11162
Size: 12358
BstEII G'GTnAC_C
Cuts at: 1184 2355 7556 1184
Size: 1171 5201 5986
Fragments arranged by size:
5986 5201 1171
BstXI CCAn_nnnn'nTGG
Cuts at: 8798 9412 8798
Size: 614 11744
BstYI r'GATC_y
Cuts at: 1972 3599 3740 5221 6871 7477 7842 9393 9688
Size: 1627 141 1481 1650 606 365 1551 295
Cuts at: 9688 10055 10440 10457 11909 11995 12007 12353 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1650 1627 1551 1481 1452 606 385
367 365 346 295 141 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2809 3436 3516 5779 6211 6565 6790
Size: 552 1744 627 80 2263 432 354 225
Cuts at: 6790 8053 8682 9064 513
Size: 1263 629 382 3807
Fragments arranged by size:
3807 2263 1744 1263 629 627 552 432
382 354 225 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2550 2709 4434 8079 487
Size: 683 56 1324 159 1725 3645 4766
Fragments arranged by size:
4766 3645 1725 1324 683 159 56
BtsI GCAGTG_nn'
Cuts at: 2914 3918 5004 7491 8096 8157 8549 8941 9225
Size: 1004 1086 2487 605 61 392 392 284
Cuts at: 9225 9296 9874 10668 10688 2914
Size: 71 578 794 20 4584
Fragments arranged by size:
4584 2487 1086 1004 794 605 578 392
392 284 71 61 20
ClaI AT'CG_AT
Cuts at: 1554 3994 1554
Size: 2440 9918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3876 3911 529
Size: 35 3312 35 8976
Fragments arranged by size:
8976 3312 35 35
DraI TTT'AAA
Cuts at: 9342 9662 9342
Size: 320 12038
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3382 9055 11365 809
Size: 507 2066 5673 2310 1802
Fragments arranged by size:
5673 2310 2066 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2311 2818 3194
Size: 54 1425 50 275 18 481 507 376
Cuts at: 3194 3801 3837 3931 7573 7947 8006 8292 9805
Size: 607 36 94 3642 374 59 286 1513
Cuts at: 9805 10694 12323 8
Size: 889 1629 43
Fragments arranged by size:
3642 1629 1513 1425 889 607 507 481
376 374 286 275 94 59 54 50
43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 9805 1537
Size: 275 7993 4090
Fragments arranged by size:
7993 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 4100 4133 5909 7346 9271 9492 10288
Size: 762 1985 33 1776 1437 1925 221 796
Cuts at: 10288 1353
Size: 3423
Fragments arranged by size:
3423 1985 1925 1776 1437 796 762 221
33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10945 11319 11465 387
Size: 1064 138 9356 374 146 1280
Fragments arranged by size:
9356 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 7603 7603
Size: 12358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2406 2667 2973 3717 4078 4157 4614
Size: 393 774 261 306 744 361 79 457
Cuts at: 4614 4957 5639 5654 6121 6666 7445 7914 8771
Size: 343 682 15 467 545 779 469 857
Cuts at: 8771 9636 10421 11018 11805 12073 1239
Size: 865 785 597 787 268 1524
Fragments arranged by size:
1524 865 857 787 785 779 774 744
682 597 545 469 467 457 393 361
343 306 268 261 79 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4617 7795 8948 4617
Size: 3178 1153 8027
Fragments arranged by size:
8027 3178 1153
EcoO109I rG'GnC_Cy
Cuts at: 2320 3212 4425 4497 4799 5319 6230 6605 6887
Size: 892 1213 72 302 520 911 375 282
Cuts at: 6887 8117 8953 9238 2320
Size: 1230 836 285 5440
Fragments arranged by size:
5440 1230 1213 911 892 836 520 375
302 285 282 72
EcoRI G'AATT_C
Cuts at: 7221 9667 10045 7221
Size: 2446 378 9534
Fragments arranged by size:
9534 2446 378
EcoRV GAT'ATC
Cuts at: 7132 7132
Size: 12358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5593 7882 8656 5593
Size: 2289 774 9295
Fragments arranged by size:
9295 2289 774
FspI TGC'GCA
Cuts at: 10864 10864
Size: 12358
HaeII r_GCGC'y
Cuts at: 1678 6336 11505 1678
Size: 4658 5169 2531
Fragments arranged by size:
5169 4658 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2120 2122 2152 4051
Size: 32 135 32 518 30 2 30 1899
Cuts at: 4051 4083 4766 4798 7928 7960 8912 8944 9101
Size: 32 683 32 3130 32 952 32 157
Cuts at: 9101 9133 9378 9410 10999 11031 11073 11105 1373
Size: 32 245 32 1589 32 42 32 2626
Fragments arranged by size:
3130 2626 1899 1589 952 683 518 245
157 135 42 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 8569 9702 9948 669
Size: 988 281 6631 1133 246 3079
Fragments arranged by size:
6631 3079 1133 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3183 3425 3749 5146 748
Size: 2435 242 324 1397 7960
Fragments arranged by size:
7960 2435 1397 324 242
HpaI GTT'AAC
Cuts at: 9948 9948
Size: 12358
KpnI G_GTAC'C
Cuts at: 6988 9683 6988
Size: 2695 9663
MfeI C'AATT_G
Cuts at: 5310 5499 9957 5310
Size: 189 4458 7711
Fragments arranged by size:
7711 4458 189
MluI A'CGCG_T
Cuts at: 12234 12234
Size: 12358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2317 4685 8458 9033 9271 9474 11472 11656
Size: 1012 2368 3773 575 238 203 1998 184
Cuts at: 11656 1305
Size: 2007
Fragments arranged by size:
3773 2368 2007 1998 1012 575 238 203
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2820 3196 3803 3839 7575
Size: 54 1425 343 988 376 607 36 3736
Cuts at: 7575 8294 10
Size: 719 4074
Fragments arranged by size:
4074 3736 1425 988 719 607 376 343
54 36
MslI CAynn'nnrTG
Cuts at: 518 1166 2491 2748 3006 3359 3996 4034 4105
Size: 648 1325 257 258 353 637 38 71
Cuts at: 4105 4107 4688 6113 6373 8249 8454 9413 10316
Size: 2 581 1425 260 1876 205 959 903
Cuts at: 10316 10675 10834 518
Size: 359 159 2042
Fragments arranged by size:
2042 1876 1425 1325 959 903 648 637
581 359 353 260 258 257 205 159
71 38 2
MspA1I CmG'CkG
Cuts at: 1606 2069 2316 2481 6141 6792 7009 7597 7671
Size: 463 247 165 3660 651 217 588 74
Cuts at: 7671 7734 8331 8364 8509 8523 9066 9774 10451
Size: 63 597 33 145 14 543 708 677
Cuts at: 10451 11599 11844 1606
Size: 1148 245 2120
Fragments arranged by size:
3660 2120 1148 708 677 651 597 588
543 463 247 245 217 165 145 74
63 33 14
NaeI GCC'GGC
Cuts at: 6019 6019
Size: 12358
NcoI C'CATG_G
Cuts at: 513 1065 3436 3516 5779 6211 6565 8682 513
Size: 552 2371 80 2263 432 354 2117 4189
Fragments arranged by size:
4189 2371 2263 2117 552 432 354 80
NdeI CA'TA_TG
Cuts at: 387 5193 387
Size: 4806 7552
NgoMIV G'CCGG_C
Cuts at: 6017 6017
Size: 12358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12358
NotI GC'GGCC_GC
Cuts at: 9805 9805
Size: 12358
NsiI A_TGCA'T
Cuts at: 3050 4298 4661 5661 5927 3050
Size: 1248 363 1000 266 9481
Fragments arranged by size:
9481 1248 1000 363 266
NspI r_CATG'y
Cuts at: 1132 1229 2796 3364 3632 4296 4401 4446 5251
Size: 97 1567 568 268 664 105 45 805
Cuts at: 5251 5659 5865 6201 6324 8244 9160 9716 11261
Size: 408 206 336 123 1920 916 556 1545
Cuts at: 11261 1132
Size: 2229
Fragments arranged by size:
2229 1920 1567 1545 916 805 664 568
556 408 336 268 206 123 105 97
45
PasI CC'CwG_GG
Cuts at: 1348 7660 1348
Size: 6312 6046
PciI A'CATG_T
Cuts at: 3360 5247 11257 3360
Size: 1887 6010 4461
Fragments arranged by size:
6010 4461 1887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5785 7497 1108
Size: 4677 1712 5969
Fragments arranged by size:
5969 4677 1712
PfoI T'CCnGG_A
Cuts at: 4225 4630 5616 8580 4225
Size: 405 986 2964 8003
Fragments arranged by size:
8003 2964 986 405
PmeI GTTT'AAAC
Cuts at: 9662 9662
Size: 12358
PmlI CAC'GTG
Cuts at: 6501 8202 6501
Size: 1701 10657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2262 5884 5916 8786 8818 10427 10459 11940
Size: 32 3622 32 2870 32 1609 32 1481
Cuts at: 11940 11972 2230
Size: 32 2616
Fragments arranged by size:
3622 2870 2616 1609 1481 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3212 4799 6230 6605 8953 9238 3212
Size: 1587 1431 375 2348 285 6332
Fragments arranged by size:
6332 2348 1587 1431 375 285
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12358
PsiI TTA'TAA
Cuts at: 9928 9928
Size: 12358
PspOMI G'GGCC_C
Cuts at: 2320 4496 2320
Size: 2176 10182
PspXI vC'TCGA_Gb
Cuts at: 9021 9021
Size: 12358
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3578 3610 5983 6015 3578
Size: 32 2373 32 9921
Fragments arranged by size:
9921 2373 32 32
PstI C_TGCA'G
Cuts at: 830 5229 6919 7586 8152 8330 8952 9291 9710
Size: 4399 1690 667 566 178 622 339 419
Cuts at: 9710 830
Size: 3478
Fragments arranged by size:
4399 3478 1690 667 622 566 419 339
178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12358
PvuII CAG'CTG
Cuts at: 2069 2481 7009 7734 8331 8364 8509 2069
Size: 412 4528 725 597 33 145 5918
Fragments arranged by size:
5918 4528 725 597 412 145 33
SacI G_AGCT'C
Cuts at: 7605 7605
Size: 12358
SacII CC_GC'GG
Cuts at: 6793 9067 6793
Size: 2274 10084
SalI G'TCGA_C
Cuts at: 1936 8567 9700 1936
Size: 6631 1133 4594
Fragments arranged by size:
6631 4594 1133
SanDI GG'GwC_CC
Cuts at: 3212 4799 3212
Size: 1587 10771
SapI GCTCTTCn'nnn_
Cuts at: 4133 9271 4133
Size: 5138 7220
SbfI CC_TGCA'GG
Cuts at: 9710 9710
Size: 12358
ScaI AGT'ACT
Cuts at: 7141 7943 10606 12318 7141
Size: 802 2663 1712 7181
Fragments arranged by size:
7181 2663 1712 802
SexAI A'CCwGG_T
Cuts at: 9260 9260
Size: 12358
SfcI C'TryA_G
Cuts at: 826 1045 2199 2880 3234 4114 4639 5225 6700
Size: 219 1154 681 354 880 525 586 1475
Cuts at: 6700 6915 7582 8148 8326 8948 9287 9706 10841
Size: 215 667 566 178 622 339 419 1135
Cuts at: 10841 11522 11713 826
Size: 681 191 1471
Fragments arranged by size:
1475 1471 1154 1135 880 681 681 667
622 586 566 525 419 354 339 219
215 191 178
SfiI GGCCn_nnn'nGGCC
Cuts at: 2319 2319
Size: 12358
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12358
SmaI CCC'GGG
Cuts at: 1872 4804 8442 9685 1872
Size: 2932 3638 1243 4545
Fragments arranged by size:
4545 3638 2932 1243
SmlI C'TyrA_G
Cuts at: 820 1017 1943 9021 9629 9646 10462 11363 11625
Size: 197 926 7078 608 17 816 901 262
Cuts at: 11625 820
Size: 1553
Fragments arranged by size:
7078 1553 926 901 816 608 262 197
17
SnaBI TAC'GTA
Cuts at: 493 2763 10149 493
Size: 2270 7386 2702
Fragments arranged by size:
7386 2702 2270
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12358
SphI G_CATG'C
Cuts at: 1132 5659 9716 1132
Size: 4527 4057 3774
Fragments arranged by size:
4527 4057 3774
SrfI GCCC'GGGC
Cuts at: 8442 8442
Size: 12358
SspI AAT'ATT
Cuts at: 5 52 5170 10282 5
Size: 47 5118 5112 2081
Fragments arranged by size:
5118 5112 2081 47
StuI AGG'CCT
Cuts at: 1517 7886 8660 9337 1517
Size: 6369 774 677 4538
Fragments arranged by size:
6369 4538 774 677
StyI C'CwwG_G
Cuts at: 513 1065 2152 3436 3516 3581 4315 5779 6211
Size: 552 1087 1284 80 65 734 1464 432
Cuts at: 6211 6226 6234 6391 6456 6565 7054 7461 7739
Size: 15 8 157 65 109 489 407 278
Cuts at: 7739 8682 9432 9797 513
Size: 943 750 365 3074
Fragments arranged by size:
3074 1464 1284 1087 943 750 734 552
489 432 407 365 278 157 109 80
65 65 15 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2141 3059 4694 5958 7607 10385 10402
Size: 1255 561 918 1635 1264 1649 2778 17
Cuts at: 10402 10555 10740 11185 325
Size: 153 185 445 1498
Fragments arranged by size:
2778 1649 1635 1498 1264 1255 918 561
445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2047 2789 4053
Size: 274 80 33 51 1443 70 742 1264
Cuts at: 4053 6749 7139 7941 10604 12316 96
Size: 2696 390 802 2663 1712 138
Fragments arranged by size:
2696 2663 1712 1443 1264 802 742 390
274 138 80 70 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4456 4488 4840 4872 6477 6509 7978 8010 12318
Size: 32 352 32 1605 32 1469 32 4308
Cuts at: 12318 12350 4456
Size: 32 4464
Fragments arranged by size:
4464 4308 1605 1469 352 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5233 9115 9176 1316
Size: 3917 3882 61 4498
Fragments arranged by size:
4498 3917 3882 61
XbaI T'CTAG_A
Cuts at: 5584 9037 9694 5584
Size: 3453 657 8248
Fragments arranged by size:
8248 3453 657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2426 2855 3320 2426
Size: 429 465 11464
Fragments arranged by size:
11464 465 429
XhoI C'TCGA_G
Cuts at: 1943 9021 1943
Size: 7078 5280
XmaI C'CCGG_G
Cuts at: 1870 4802 8440 9683 1870
Size: 2932 3638 1243 4545
Fragments arranged by size:
4545 3638 2932 1243
XmnI GAAnn'nnTTC
Cuts at: 3254 5147 10487 3254
Size: 1893 5340 5125
Fragments arranged by size:
5340 5125 1893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5399 5596 10166 276
Size: 53 83 186 936 3865 197 4570 2468
Fragments arranged by size:
4570 3865 2468 936 197 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BsiWI BstZ17I Bsu36I DraIII
FseI FspAI KasI NarI NruI PacI RsrII SfoI
SwaI