Restriction map for
pFN21AE2560
(Circular) (Six-base) MAPSORT of: pFN21AE2560.seq Check: 3504 from: 1 to: 5332
pFN21AE2560 5332 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3142 278
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2604 2675 1937
Size: 667 71 4594
Fragments arranged by size:
4594 667 71
Acc65I G'GTAC_C
Cuts at: 2653 2653
Size: 5332
AclI AA'CG_TT
Cuts at: 1563 2269 3459 3832 1563
Size: 706 1190 373 3063
Fragments arranged by size:
3063 1190 706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 2640 3395 4779 5047 1632
Size: 900 108 755 1384 268 1917
Fragments arranged by size:
1917 1384 900 755 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5135
AflIII A'CryG_T
Cuts at: 1203 2197 2356 4231 5208 1203
Size: 994 159 1875 977 1327
Fragments arranged by size:
1875 1327 994 977 159
AgeI A'CCGG_T
Cuts at: 4163 4163
Size: 5332
AhdI GACnn_n'nnGTC
Cuts at: 4061 4061
Size: 5332
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5332
AlwNI CAG_nnn'CTG
Cuts at: 3075 4647 3075
Size: 1572 3760
ApaLI G'TGCA_C
Cuts at: 3389 4545 3389
Size: 1156 4176
ApoI r'AATT_y
Cuts at: 1493 2281 2641 2867 3019 5128 1493
Size: 788 360 226 152 2109 1697
Fragments arranged by size:
2109 1697 788 360 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3886 160
Size: 872 2854 1606
Fragments arranged by size:
2854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2099 2657 1870
Size: 73 131 25 558 4545
Fragments arranged by size:
4545 558 131 73 25
BamHI G'GATC_C
Cuts at: 2662 3029 2662
Size: 367 4965
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 2653 4108 618
Size: 325 217 627 266 600 1455 1842
Fragments arranged by size:
1842 1455 627 600 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2651 1391
Size: 1260 4072
BbeI G_GCGC'C
Cuts at: 2057 2057
Size: 5332
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3523 3557 1903
Size: 34 1586 34 3678
Fragments arranged by size:
3678 1586 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3224 4434 3224
Size: 1210 4122
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5332
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3943 136
Size: 107 122 71 631 605 2271 1525
Fragments arranged by size:
2271 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2179 5327 2179
Size: 3148 2184
BlpI GC'TnA_GC
Cuts at: 2749 2749
Size: 5332
Bme1580I G_kGCm'C
Cuts at: 1790 3393 4549 1790
Size: 1603 1156 2573
Fragments arranged by size:
2573 1603 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3063 4021 442
Size: 416 53 83 399 24 1646 958 1753
Fragments arranged by size:
1753 1646 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5332
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 2392 2031
Size: 361 4971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3992 1239
Size: 2753 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3457 4322 4620 3457
Size: 865 298 4169
Fragments arranged by size:
4169 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3995 882
Size: 617 2496 2219
Fragments arranged by size:
2496 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2359 3123 493
Size: 711 1155 764 2702
Fragments arranged by size:
2702 1155 764 711
BsaBI GATnn'nnATC
Cuts at: 5326 5326
Size: 5332
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 3139 3521 4156
Size: 53 83 186 936 521 1085 382 635
Cuts at: 4156 275
Size: 1451
Fragments arranged by size:
1451 1085 936 635 521 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2065 3765 4163 4437 4584 4995 2065
Size: 1700 398 274 147 411 2402
Fragments arranged by size:
2402 1700 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5107
Fragments arranged by size:
5107 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5332
BseYI C'CCAG_C
Cuts at: 4535 5031 4535
Size: 496 4836
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4759
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2782 3543 4571 1540
Size: 275 186 781 761 1028 2301
Fragments arranged by size:
2301 1028 781 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3393 3478 4549 3393
Size: 85 1071 4176
Fragments arranged by size:
4176 1071 85
BsmI GAATG_Cn'
Cuts at: 2161 2843 2936 2161
Size: 682 93 4557
Fragments arranged by size:
4557 682 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2651 3393 3478 4549 1391
Size: 399 861 742 85 1071 2174
Fragments arranged by size:
2174 1071 861 742 399 85
BspEI T'CCGG_A
Cuts at: 2065 4995 2065
Size: 2930 2402
BspHI T'CATG_A
Cuts at: 3221 4951 3221
Size: 1730 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2687 844
Size: 1843 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3219 1394
Size: 429 1396 3507
Fragments arranged by size:
3507 1396 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3827 4001 65
Size: 1183 2579 174 1396
Fragments arranged by size:
2579 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3976 4163 1700
Size: 110 2166 187 2869
Fragments arranged by size:
2869 2166 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5332
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5332
BssSI C'ACGA_G
Cuts at: 3392 4404 3392
Size: 1012 4320
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5332
BstBI TT'CG_AA
Cuts at: 4136 4136
Size: 5332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5332
BstYI r'GATC_y
Cuts at: 1972 2179 2662 3029 3414 3431 4883 4969 4981
Size: 207 483 367 385 17 1452 86 12
Cuts at: 4981 5327 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 483 385 367 346 207 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4780
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4593
Fragments arranged by size:
4593 683 56
BtsI GCAGTG_nn'
Cuts at: 2848 3642 3662 2848
Size: 794 20 4518
Fragments arranged by size:
4518 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5297
DraI TTT'AAA
Cuts at: 2242 2565 2636 2242
Size: 323 71 4938
Fragments arranged by size:
4938 323 71
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4339 809
Size: 507 3023 1802
Fragments arranged by size:
3023 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2779 3668 5297
Size: 54 1425 50 275 18 949 889 1629
Cuts at: 5297 8
Size: 43
Fragments arranged by size:
1629 1425 949 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2779 1537
Size: 275 967 4090
Fragments arranged by size:
4090 967 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2574 3262 1353
Size: 1221 688 3423
Fragments arranged by size:
3423 1221 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3919 4293 4439 387
Size: 1064 138 2330 374 146 1280
Fragments arranged by size:
2330 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 2640 3395 3992 4779 5047 1239
Size: 393 900 108 755 597 787 268 1524
Fragments arranged by size:
1524 900 787 755 597 393 268 108
EcoRI G'AATT_C
Cuts at: 2641 3019 2641
Size: 378 4954
EcoRV GAT'ATC
Cuts at: 2518 2518
Size: 5332
FspI TGC'GCA
Cuts at: 3838 3838
Size: 5332
HaeII r_GCGC'y
Cuts at: 1678 2057 4479 1678
Size: 379 2422 2531
Fragments arranged by size:
2531 2422 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2123 2155 3973 4005 4047
Size: 32 135 32 551 32 1818 32 42
Cuts at: 4047 4079 1373
Size: 32 2626
Fragments arranged by size:
2626 1818 551 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2676 2922 669
Size: 988 281 738 246 3079
Fragments arranged by size:
3079 988 738 281 246
HindIII A'AGCT_T
Cuts at: 748 2105 748
Size: 1357 3975
HpaI GTT'AAC
Cuts at: 2922 2922
Size: 5332
KasI G'GCGC_C
Cuts at: 2053 2053
Size: 5332
KpnI G_GTAC'C
Cuts at: 2657 2657
Size: 5332
MfeI C'AATT_G
Cuts at: 2931 2931
Size: 5332
MluI A'CGCG_T
Cuts at: 5208 5208
Size: 5332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4446 4630 1305
Size: 3141 184 2007
Fragments arranged by size:
3141 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3510
Fragments arranged by size:
3510 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3290 3649 3808 518
Size: 648 2124 359 159 2042
Fragments arranged by size:
2124 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2748 3425 4573 4818 1606
Size: 1142 677 1148 245 2120
Fragments arranged by size:
2120 1148 1142 677 245
NarI GG'CG_CC
Cuts at: 2054 2054
Size: 5332
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4780
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5332
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5332
NotI GC'GGCC_GC
Cuts at: 2779 2779
Size: 5332
NspI r_CATG'y
Cuts at: 1132 1229 2201 2690 4235 1132
Size: 97 972 489 1545 2229
Fragments arranged by size:
2229 1545 972 489 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5332
PciI A'CATG_T
Cuts at: 2197 4231 2197
Size: 2034 3298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5332
PmeI GTTT'AAAC
Cuts at: 2636 2636
Size: 5332
PmlI CAC'GTG
Cuts at: 2359 2359
Size: 5332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 4914 4946 3401
Size: 32 1481 32 3787
Fragments arranged by size:
3787 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5332
PsiI TTA'TAA
Cuts at: 2414 2505 2902 2414
Size: 91 397 4844
Fragments arranged by size:
4844 397 91
PstI C_TGCA'G
Cuts at: 830 2684 830
Size: 1854 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5332
SalI G'TCGA_C
Cuts at: 1936 2674 1936
Size: 738 4594
SbfI CC_TGCA'GG
Cuts at: 2684 2684
Size: 5332
ScaI AGT'ACT
Cuts at: 2263 3580 5292 2263
Size: 1317 1712 2303
Fragments arranged by size:
2303 1712 1317
SfcI C'TryA_G
Cuts at: 826 1045 2183 2680 3815 4496 4687 826
Size: 219 1138 497 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 681 497 219 191
SfoI GGC'GCC
Cuts at: 2055 2055
Size: 5332
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5332
SmaI CCC'GGG
Cuts at: 1872 2076 2659 1872
Size: 204 583 4545
Fragments arranged by size:
4545 583 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2099 3436 4337 4599 820
Size: 197 926 156 1337 901 262 1553
Fragments arranged by size:
1553 1337 926 901 262 197 156
SnaBI TAC'GTA
Cuts at: 493 3123 493
Size: 2630 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5332
SphI G_CATG'C
Cuts at: 1132 2690 1132
Size: 1558 3774
SspI AAT'ATT
Cuts at: 5 52 3256 5
Size: 47 3204 2081
Fragments arranged by size:
3204 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5332
StyI C'CwwG_G
Cuts at: 513 1065 2771 513
Size: 552 1706 3074
Fragments arranged by size:
3074 1706 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3359 3376 3529 3714 4159 325
Size: 1255 1779 17 153 185 445 1498
Fragments arranged by size:
1779 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2261 2322 3578
Size: 274 80 33 51 1443 284 61 1256
Cuts at: 3578 5290 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1256 284 274 138 80 61
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5292 5324 5292
Size: 32 5300
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5332
XbaI T'CTAG_A
Cuts at: 2668 2668
Size: 5332
XhoI C'TCGA_G
Cuts at: 1943 2099 1943
Size: 156 5176
XmaI C'CCGG_G
Cuts at: 1870 2074 2657 1870
Size: 204 583 4545
Fragments arranged by size:
4545 583 204
XmnI GAAnn'nnTTC
Cuts at: 3461 3461
Size: 5332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3140 276
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI