Restriction map for
pFN21AE2557
(Circular) (Six-base) MAPSORT of: pFN21AE2557.seq Check: 482 from: 1 to: 7546
pFN21AE2557 7546 bp
With 182 enzymes: SgfI *
December 15, 2014 13:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3445 3445
Size: 7546
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3279 5356 278
Size: 53 83 186 936 1743 2077 2468
Fragments arranged by size:
2468 2077 1743 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4667 4889 1937
Size: 2730 222 4594
Fragments arranged by size:
4594 2730 222
Acc65I G'GTAC_C
Cuts at: 3598 4867 3598
Size: 1269 6277
AclI AA'CG_TT
Cuts at: 1563 5673 6046 1563
Size: 4110 373 3063
Fragments arranged by size:
4110 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2050 2606 2744 2849 4248 4277 5609 6993
Size: 418 556 138 105 1399 29 1332 1384
Cuts at: 6993 7261 1632
Size: 268 1917
Fragments arranged by size:
1917 1399 1384 1332 556 418 268 138
105 29
AflII C'TTAA_G
Cuts at: 820 1017 4532 820
Size: 197 3515 3834
Fragments arranged by size:
3834 3515 197
AflIII A'CryG_T
Cuts at: 1203 6445 7422 1203
Size: 5242 977 1327
Fragments arranged by size:
5242 1327 977
AgeI A'CCGG_T
Cuts at: 6377 6377
Size: 7546
AhdI GACnn_n'nnGTC
Cuts at: 2917 6275 2917
Size: 3358 4188
AleI CACnn'nnGTG
Cuts at: 1166 3437 1166
Size: 2271 5275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 4113 1184
Size: 1116 1813 4617
Fragments arranged by size:
4617 1813 1116
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2418 2450 2418
Size: 32 7514
AlwNI CAG_nnn'CTG
Cuts at: 3196 3515 5289 6861 3196
Size: 319 1774 1572 3881
Fragments arranged by size:
3881 1774 1572 319
ApaI G_GGCC'C
Cuts at: 2229 2411 3986 4761 2229
Size: 182 1575 775 5014
Fragments arranged by size:
5014 1575 775 182
ApaLI G'TGCA_C
Cuts at: 3086 5603 6759 3086
Size: 2517 1156 3873
Fragments arranged by size:
3873 2517 1156
ApoI r'AATT_y
Cuts at: 1493 4436 4714 4855 5081 5233 7342 1493
Size: 2943 278 141 226 152 2109 1697
Fragments arranged by size:
2943 2109 1697 278 226 152 141
AseI AT'TA_AT
Cuts at: 160 1032 6100 160
Size: 872 5068 1606
Fragments arranged by size:
5068 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 2238 3270 4566 4871 1870
Size: 73 109 186 1032 1296 305 4545
Fragments arranged by size:
4545 1296 1032 305 186 109 73
BamHI G'GATC_C
Cuts at: 4876 5243 4876
Size: 367 7179
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2923 3265 3374 3416 3598
Size: 325 217 627 1136 342 109 42 182
Cuts at: 3598 4867 6322 618
Size: 1269 1455 1842
Fragments arranged by size:
1842 1455 1269 1136 627 342 325 217
182 109 42
BanII G_rGCy'C
Cuts at: 1391 2229 2411 3986 4307 4761 4845 4865 1391
Size: 838 182 1575 321 454 84 20 4072
Fragments arranged by size:
4072 1575 838 454 321 182 84 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2388 928
Size: 971 489 6086
Fragments arranged by size:
6086 971 489
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5737 5771 1903
Size: 34 50 34 3716 34 3678
Fragments arranged by size:
3716 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3926 5438 6648 3926
Size: 1512 1210 4824
Fragments arranged by size:
4824 1512 1210
BclI T'GATC_A
Cuts at: 1548 3745 1548
Size: 2197 5349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3990 6157 136
Size: 107 122 71 631 605 2318 2167 1525
Fragments arranged by size:
2318 2167 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2163 7541 2163
Size: 5378 2168
BlpI GC'TnA_GC
Cuts at: 3116 4739 4963 3116
Size: 1623 224 5699
Fragments arranged by size:
5699 1623 224
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2411 2928 3090 3217 3377 3986 4761
Size: 439 182 517 162 127 160 609 775
Cuts at: 4761 5607 6763 1790
Size: 846 1156 2573
Fragments arranged by size:
2573 1156 846 775 609 517 439 182
162 160 127
BmgBI CAC'GTC
Cuts at: 1318 2089 1318
Size: 771 6775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2311 2356 2414
Size: 416 53 83 399 24 894 45 58
Cuts at: 2414 3637 3727 3938 3997 5277 6235 442
Size: 1223 90 211 59 1280 958 1753
Fragments arranged by size:
1753 1280 1223 958 894 416 399 211
90 83 59 58 53 45 24
BmtI G_CTAG'C
Cuts at: 1056 2159 1056
Size: 1103 6443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2711 2837 2894 2936 2993 3003 3239 3347
Size: 1472 126 57 42 57 10 236 108
Cuts at: 3347 3396 3965 4661 6206 1239
Size: 49 569 696 1545 2579
Fragments arranged by size:
2579 1545 1472 696 569 236 126 108
57 57 49 42 10
Bpu10I CC'TnA_GC
Cuts at: 2676 3137 4301 2676
Size: 461 1164 5921
Fragments arranged by size:
5921 1164 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3290 5671 6536 6834 3290
Size: 2381 865 298 4002
Fragments arranged by size:
4002 2381 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2656 3730 3867 4041 6209 882
Size: 617 1157 1074 137 174 2168 2219
Fragments arranged by size:
2219 2168 1157 1074 617 174 137
BsaAI yAC'GTr
Cuts at: 493 1204 5337 493
Size: 711 4133 2702
Fragments arranged by size:
4133 2702 711
BsaBI GATnn'nnATC
Cuts at: 7540 7540
Size: 7546
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3276 4320 5353 5735
Size: 53 83 186 936 1743 1044 1033 382
Cuts at: 5735 6370 275
Size: 635 1451
Fragments arranged by size:
1743 1451 1044 1033 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2621 3318 4335 5979 6377 6651 6798 7209 2621
Size: 697 1017 1644 398 274 147 411 2958
Fragments arranged by size:
2958 1644 1017 697 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4814 4844 1178
Size: 30 165 30 3411 30 3880
Fragments arranged by size:
3880 3411 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2122 4267 1186
Size: 936 2145 4465
Fragments arranged by size:
4465 2145 936
BseYI C'CCAG_C
Cuts at: 2079 2119 2467 2491 2707 2811 3578 3886 4131
Size: 40 348 24 216 104 767 308 245
Cuts at: 4131 6749 7245 2079
Size: 2618 496 2380
Fragments arranged by size:
2618 2380 767 496 348 308 245 216
104 40 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2562 2852 4520 1163
Size: 573 826 290 1668 4189
Fragments arranged by size:
4189 1668 826 573 290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2077 3319 4996 5757 6785 1540
Size: 275 186 76 1242 1677 761 1028 2301
Fragments arranged by size:
2301 1677 1242 1028 761 275 186 76
BsiHKAI G_wGCw'C
Cuts at: 2481 3090 4845 5607 5692 6763 2481
Size: 609 1755 762 85 1071 3264
Fragments arranged by size:
3264 1755 1071 762 609 85
BsmI GAATG_Cn'
Cuts at: 2423 5057 5150 2423
Size: 2634 93 4819
Fragments arranged by size:
4819 2634 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2411 2481 2928 3090 3217 3377
Size: 399 439 182 70 447 162 127 160
Cuts at: 3377 3986 4307 4761 4845 4865 5607 5692 6763
Size: 609 321 454 84 20 742 85 1071
Cuts at: 6763 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 609 454 447 439 399
321 182 162 160 127 85 84 70
20
BspEI T'CCGG_A
Cuts at: 7209 7209
Size: 7546
BspHI T'CATG_A
Cuts at: 5435 7165 5435
Size: 1730 5816
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3445 3708 4707 4901 844
Size: 2601 263 999 194 3489
Fragments arranged by size:
3489 2601 999 263 194
BsrBI CCG'CTC
Cuts at: 1394 1823 3949 4789 5433 1394
Size: 429 2126 840 644 3507
Fragments arranged by size:
3507 2126 840 644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2535 6041 6215 65
Size: 1183 1287 3506 174 1396
Fragments arranged by size:
3506 1396 1287 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2522 3419 3713 6190 6377 1700
Size: 110 712 897 294 2477 187 2869
Fragments arranged by size:
2869 2477 897 712 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 3972 96
Size: 3876 3670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7546
BssSI C'ACGA_G
Cuts at: 5606 6618 5606
Size: 1012 6534
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3862 794
Size: 3068 4478
BstBI TT'CG_AA
Cuts at: 6350 6350
Size: 7546
BstEII G'GTnAC_C
Cuts at: 1184 2635 1184
Size: 1451 6095
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 3923 4051 2455
Size: 1468 128 5950
Fragments arranged by size:
5950 1468 128
BstYI r'GATC_y
Cuts at: 1972 2163 4507 4876 5243 5628 5645 7097 7183
Size: 191 2344 369 367 385 17 1452 86
Cuts at: 7183 7195 7541 1972
Size: 12 346 1977
Fragments arranged by size:
2344 1977 1452 385 369 367 346 191
86 17 12
BstZ17I GTA'TAC
Cuts at: 4668 4668
Size: 7546
Bsu36I CC'TnA_GG
Cuts at: 2294 2735 2787 3603 4753 2294
Size: 441 52 816 1150 5087
Fragments arranged by size:
5087 1150 816 441 52
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6994
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6807
Fragments arranged by size:
6807 683 56
BtsI GCAGTG_nn'
Cuts at: 2150 2778 3708 3781 3938 5062 5856 5876 2150
Size: 628 930 73 157 1124 794 20 3820
Fragments arranged by size:
3820 1124 930 794 628 157 73 20
ClaI AT'CG_AT
Cuts at: 1554 3768 1554
Size: 2214 5332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7511
DraI TTT'AAA
Cuts at: 4850 4850
Size: 7546
DraIII CAC_nnn'GTG
Cuts at: 3094 3094
Size: 7546
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6553 809
Size: 507 5237 1802
Fragments arranged by size:
5237 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3778 4993 5882
Size: 54 1425 50 275 18 1948 1215 889
Cuts at: 5882 7511 8
Size: 1629 43
Fragments arranged by size:
1948 1629 1425 1215 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4993 1537
Size: 275 3181 4090
Fragments arranged by size:
4090 3181 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2345 4224 5476 1353
Size: 992 1879 1252 3423
Fragments arranged by size:
3423 1879 1252 992
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 2325 6133 6507 6653 387
Size: 1064 138 660 76 3808 374 146 1280
Fragments arranged by size:
3808 1280 1064 660 374 146 138 76
EcoICRI GAG'CTC
Cuts at: 4843 4843
Size: 7546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2606 2711 2744 2837 2849 2894
Size: 393 418 556 105 33 93 12 45
Cuts at: 2894 2936 2993 3003 3239 3347 3396 3965 4248
Size: 42 57 10 236 108 49 569 283
Cuts at: 4248 4277 4661 5609 6206 6993 7261 1239
Size: 29 384 948 597 787 268 1524
Fragments arranged by size:
1524 948 787 597 569 556 418 393
384 283 268 236 108 105 93 57
49 45 42 33 29 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 4703 4781 4703
Size: 78 7468
EcoO109I rG'GnC_Cy
Cuts at: 2225 2226 2408 2495 2938 3061 3890 3982 3983
Size: 1 182 87 443 123 829 92 1
Cuts at: 3983 4576 4757 4758 4804 2225
Size: 593 181 1 46 4967
Fragments arranged by size:
4967 829 593 443 182 181 123 92
87 46 1 1 1
EcoRI G'AATT_C
Cuts at: 4436 4855 5233 4436
Size: 419 378 6749
Fragments arranged by size:
6749 419 378
EcoRV GAT'ATC
Cuts at: 2131 2899 2131
Size: 768 6778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 3136 3190 2467
Size: 669 54 6823
Fragments arranged by size:
6823 669 54
FspI TGC'GCA
Cuts at: 6052 6052
Size: 7546
HaeII r_GCGC'y
Cuts at: 1678 2708 3235 6693 1678
Size: 1030 527 3458 2531
Fragments arranged by size:
3458 2531 1030 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2014 2046 2903 2935 6187
Size: 32 135 32 442 32 857 32 3252
Cuts at: 6187 6219 6261 6293 1373
Size: 32 42 32 2626
Fragments arranged by size:
3252 2626 857 442 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4890 5136 669
Size: 988 281 2952 246 3079
Fragments arranged by size:
3079 2952 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7546
HpaI GTT'AAC
Cuts at: 5136 5136
Size: 7546
KpnI G_GTAC'C
Cuts at: 3602 4871 3602
Size: 1269 6277
MfeI C'AATT_G
Cuts at: 5145 5145
Size: 7546
MluI A'CGCG_T
Cuts at: 7422 7422
Size: 7546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3528 6660 6844 1305
Size: 2223 3132 184 2007
Fragments arranged by size:
3132 2223 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3780 10
Size: 54 1425 343 1948 3776
Fragments arranged by size:
3776 1948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3437 3593 4589 4767 5504 5863 6022
Size: 648 2271 156 996 178 737 359 159
Cuts at: 6022 518
Size: 2042
Fragments arranged by size:
2271 2042 996 737 648 359 178 159
156
MspA1I CmG'CkG
Cuts at: 1606 2079 2584 2743 2815 3196 3283 3346 3859
Size: 473 505 159 72 381 87 63 513
Cuts at: 3859 3886 4131 4504 4962 5639 6787 7032 1606
Size: 27 245 373 458 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 513 505 473 458 381
373 245 245 159 87 72 63 27
NaeI GCC'GGC
Cuts at: 2524 3715 2524
Size: 1191 6355
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6994
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7546
NgoMIV G'CCGG_C
Cuts at: 2522 3713 2522
Size: 1191 6355
NheI G'CTAG_C
Cuts at: 1052 2155 1052
Size: 1103 6443
NotI GC'GGCC_GC
Cuts at: 4993 4993
Size: 7546
NspI r_CATG'y
Cuts at: 1132 1229 4904 6449 1132
Size: 97 3675 1545 2229
Fragments arranged by size:
3675 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7546
PciI A'CATG_T
Cuts at: 6445 6445
Size: 7546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2934 3487 3787 1108
Size: 1826 553 300 4867
Fragments arranged by size:
4867 1826 553 300
PfoI T'CCnGG_A
Cuts at: 2980 3847 4050 4638 2980
Size: 867 203 588 5888
Fragments arranged by size:
5888 867 588 203
PmeI GTTT'AAAC
Cuts at: 4850 4850
Size: 7546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5615 5647 7128 7160 5615
Size: 32 1481 32 6001
Fragments arranged by size:
6001 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2495 2938 3061 2495
Size: 443 123 6980
Fragments arranged by size:
6980 443 123
PshAI GACnn'nnGTC
Cuts at: 1613 2054 3321 1613
Size: 441 1267 5838
Fragments arranged by size:
5838 1267 441
PsiI TTA'TAA
Cuts at: 5116 5116
Size: 7546
PspOMI G'GGCC_C
Cuts at: 2225 2407 3982 4757 2225
Size: 182 1575 775 5014
Fragments arranged by size:
5014 1575 775 182
PstI C_TGCA'G
Cuts at: 830 2886 3351 3678 4092 4898 830
Size: 2056 465 327 414 806 3478
Fragments arranged by size:
3478 2056 806 465 414 327
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7546
PvuII CAG'CTG
Cuts at: 2584 2743 2815 3346 3859 3886 4504 2584
Size: 159 72 531 513 27 618 5626
Fragments arranged by size:
5626 618 531 513 159 72 27
SacI G_AGCT'C
Cuts at: 4845 4845
Size: 7546
SalI G'TCGA_C
Cuts at: 1936 4888 1936
Size: 2952 4594
SanDI GG'GwC_CC
Cuts at: 2938 2938
Size: 7546
SbfI CC_TGCA'GG
Cuts at: 4898 4898
Size: 7546
ScaI AGT'ACT
Cuts at: 5794 7506 5794
Size: 1712 5834
SfcI C'TryA_G
Cuts at: 826 1045 2882 3347 3674 3810 4030 4088 4894
Size: 219 1837 465 327 136 220 58 806
Cuts at: 4894 6029 6710 6901 826
Size: 1135 681 191 1471
Fragments arranged by size:
1837 1471 1135 806 681 465 327 220
219 191 136 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7546
SmaI CCC'GGG
Cuts at: 1872 4568 4873 1872
Size: 2696 305 4545
Fragments arranged by size:
4545 2696 305
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3305 4532 5650 6551 6813 820
Size: 197 926 1362 1227 1118 901 262 1553
Fragments arranged by size:
1553 1362 1227 1118 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5337 493
Size: 4844 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7546
SphI G_CATG'C
Cuts at: 1132 4904 1132
Size: 3772 3774
SspI AAT'ATT
Cuts at: 5 52 5470 5
Size: 47 5418 2081
Fragments arranged by size:
5418 2081 47
StuI AGG'CCT
Cuts at: 1517 4094 1517
Size: 2577 4969
StyI C'CwwG_G
Cuts at: 513 1065 4441 4572 4985 513
Size: 552 3376 131 413 3074
Fragments arranged by size:
3376 3074 552 413 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5573 5590 5743 5928 6373 325
Size: 1255 3993 17 153 185 445 1498
Fragments arranged by size:
3993 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3972 5792 7504
Size: 274 80 33 51 1443 1995 1820 1712
Cuts at: 7504 96
Size: 138
Fragments arranged by size:
1995 1820 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2188 2220 2616 2648 3367 3399 4814 4846 7506
Size: 32 396 32 719 32 1415 32 2660
Cuts at: 7506 7538 2188
Size: 32 2196
Fragments arranged by size:
2660 2196 1415 719 396 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3107 1316
Size: 1791 5755
XbaI T'CTAG_A
Cuts at: 4882 4882
Size: 7546
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7546
XmaI C'CCGG_G
Cuts at: 1870 4566 4871 1870
Size: 2696 305 4545
Fragments arranged by size:
4545 2696 305
XmnI GAAnn'nnTTC
Cuts at: 4634 5675 4634
Size: 1041 6505
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3277 5354 276
Size: 53 83 186 936 1743 2077 2468
Fragments arranged by size:
2468 2077 1743 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI