Restriction map for
pFN21AE2550
(Circular) (Six-base) MAPSORT of: pFN21AE2550.seq Check: 3758 from: 1 to: 5854
pFN21AE2550 5854 bp
With 182 enzymes: SgfI *
January 27, 2015 16:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2855 2855
Size: 5854
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3664 278
Size: 53 83 186 936 2128 2468
Fragments arranged by size:
2468 2128 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3197 1937
Size: 1260 4594
Acc65I G'GTAC_C
Cuts at: 3175 3175
Size: 5854
AclI AA'CG_TT
Cuts at: 1563 3981 4354 1563
Size: 2418 373 3063
Fragments arranged by size:
3063 2418 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2897 3917 5301 5569 1632
Size: 1265 1020 1384 268 1917
Fragments arranged by size:
1917 1384 1265 1020 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5657
AflIII A'CryG_T
Cuts at: 1203 4753 5730 1203
Size: 3550 977 1327
Fragments arranged by size:
3550 1327 977
AgeI A'CCGG_T
Cuts at: 4685 4685
Size: 5854
AhdI GACnn_n'nnGTC
Cuts at: 4583 4583
Size: 5854
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5854
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 5822
AlwNI CAG_nnn'CTG
Cuts at: 3049 3112 3597 5169 3049
Size: 63 485 1572 3734
Fragments arranged by size:
3734 1572 485 63
ApaI G_GGCC'C
Cuts at: 2018 2018
Size: 5854
ApaLI G'TGCA_C
Cuts at: 3911 5067 3911
Size: 1156 4698
ApoI r'AATT_y
Cuts at: 1493 2503 3163 3389 3541 5650 1493
Size: 1010 660 226 152 2109 1697
Fragments arranged by size:
2109 1697 1010 660 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4408 160
Size: 872 3376 1606
Fragments arranged by size:
3376 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
AvaI C'yCGr_G
Cuts at: 1870 1943 3179 1870
Size: 73 1236 4545
Fragments arranged by size:
4545 1236 73
BamHI G'GATC_C
Cuts at: 3184 3551 3184
Size: 367 5487
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3149 3175 4630 618
Size: 325 217 627 1362 26 1455 1842
Fragments arranged by size:
1842 1455 1362 627 325 217 26
BanII G_rGCy'C
Cuts at: 1391 2018 2079 2393 2421 2479 3033 3173 1391
Size: 627 61 314 28 58 554 140 4072
Fragments arranged by size:
4072 627 554 314 140 61 58 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2014 2078 2379 2534 928
Size: 971 115 64 301 155 4248
Fragments arranged by size:
4248 971 301 155 115 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4045 4079 1903
Size: 34 2108 34 3678
Fragments arranged by size:
3678 2108 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3746 4956 3746
Size: 1210 4644
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5854
BfrBI ATG'CAT
Cuts at: 2554 2554
Size: 5854
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4465 136
Size: 107 122 71 631 605 2793 1525
Fragments arranged by size:
2793 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3121 5849 3121
Size: 2728 3126
BlpI GC'TnA_GC
Cuts at: 2073 3271 2073
Size: 1198 4656
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2622 2892 3152 3915 5071 1790
Size: 228 604 270 260 763 1156 2573
Fragments arranged by size:
2573 1156 763 604 270 260 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5854
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 3056 3585
Size: 416 53 83 399 24 1041 598 529
Cuts at: 3585 4543 442
Size: 958 1753
Fragments arranged by size:
1753 1041 958 598 529 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2455 2459 4514 1239
Size: 1088 128 4 2055 2579
Fragments arranged by size:
2579 2055 1088 128 4
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3979 4844 5142 3979
Size: 865 298 4691
Fragments arranged by size:
4691 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2252 4517 882
Size: 617 753 2265 2219
Fragments arranged by size:
2265 2219 753 617
BsaAI yAC'GTr
Cuts at: 493 1204 3645 493
Size: 711 2441 2702
Fragments arranged by size:
2702 2441 711
BsaBI GATnn'nnATC
Cuts at: 5848 5848
Size: 5854
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2722 3661 4043 4678
Size: 53 83 186 936 1189 939 382 635
Cuts at: 4678 275
Size: 1451
Fragments arranged by size:
1451 1189 939 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4287 4685 4959 5106 5517 4287
Size: 398 274 147 411 4624
Fragments arranged by size:
4624 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2825 2855 1178
Size: 30 165 30 1422 30 4177
Fragments arranged by size:
4177 1422 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2623 2846 1186
Size: 1437 223 4194
Fragments arranged by size:
4194 1437 223
BseYI C'CCAG_C
Cuts at: 2011 2394 2754 5057 5553 2011
Size: 383 360 2303 496 2312
Fragments arranged by size:
2312 2303 496 383 360
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 1163
Size: 573 411 4870
Fragments arranged by size:
4870 573 411
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3304 4065 5093 1540
Size: 275 186 1303 761 1028 2301
Fragments arranged by size:
2301 1303 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2393 2421 2479 3033 3915 4000 5071 2393
Size: 28 58 554 882 85 1071 3176
Fragments arranged by size:
3176 1071 882 554 85 58 28
BsmI GAATG_Cn'
Cuts at: 2335 3365 3458 2335
Size: 1030 93 4731
Fragments arranged by size:
4731 1030 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2079 2393 2421 2479 2622 2892
Size: 399 228 61 314 28 58 143 270
Cuts at: 2892 3033 3152 3173 3915 4000 5071 1391
Size: 141 119 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 314 270 228 143
141 119 85 61 58 28 21
BspEI T'CCGG_A
Cuts at: 5517 5517
Size: 5854
BspHI T'CATG_A
Cuts at: 3743 5473 3743
Size: 1730 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2855 3034 3209 844
Size: 2011 179 175 3489
Fragments arranged by size:
3489 2011 179 175
BsrBI CCG'CTC
Cuts at: 1394 1823 3741 1394
Size: 429 1918 3507
Fragments arranged by size:
3507 1918 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4349 4523 65
Size: 1183 3101 174 1396
Fragments arranged by size:
3101 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4498 4685 1700
Size: 110 2688 187 2869
Fragments arranged by size:
2869 2688 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5854
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5854
BssSI C'ACGA_G
Cuts at: 3032 3914 4926 3032
Size: 882 1012 3960
Fragments arranged by size:
3960 1012 882
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5854
BstBI TT'CG_AA
Cuts at: 4658 4658
Size: 5854
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5854
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5854
BstYI r'GATC_y
Cuts at: 1972 2120 2364 3121 3184 3551 3936 3953 5405
Size: 148 244 757 63 367 385 17 1452
Cuts at: 5405 5491 5503 5849 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 757 385 367 346 244 148
86 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 2291 2606 2291
Size: 315 5539
BtgI C'CryG_G
Cuts at: 513 1065 2004 2625 513
Size: 552 939 621 3742
Fragments arranged by size:
3742 939 621 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5115
Fragments arranged by size:
5115 683 56
BtsI GCAGTG_nn'
Cuts at: 2765 3370 4164 4184 2765
Size: 605 794 20 4435
Fragments arranged by size:
4435 794 605 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5854
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5819
DraI TTT'AAA
Cuts at: 3158 3158
Size: 5854
DraIII CAC_nnn'GTG
Cuts at: 2887 2887
Size: 5854
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4861 809
Size: 507 3545 1802
Fragments arranged by size:
3545 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2487 2808 3301
Size: 54 1425 50 275 18 657 321 493
Cuts at: 3301 4190 5819 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 657 493 321 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3301 1537
Size: 275 1489 4090
Fragments arranged by size:
4090 1489 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2426 3784 1353
Size: 1073 1358 3423
Fragments arranged by size:
3423 1358 1073
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4441 4815 4961 387
Size: 1064 138 2852 374 146 1280
Fragments arranged by size:
2852 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2391 2419 2477 3031 2391
Size: 28 58 554 5214
Fragments arranged by size:
5214 554 58 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2455 2459 2897 3917 4514 5301
Size: 393 695 128 4 438 1020 597 787
Cuts at: 5301 5569 1239
Size: 268 1524
Fragments arranged by size:
1524 1020 787 695 597 438 393 268
128 4
EcoO109I rG'GnC_Cy
Cuts at: 2287 3046 2287
Size: 759 5095
EcoRI G'AATT_C
Cuts at: 3163 3541 3163
Size: 378 5476
EcoRV GAT'ATC
Cuts at: 2585 2585
Size: 5854
FspI TGC'GCA
Cuts at: 4360 4360
Size: 5854
HaeII r_GCGC'y
Cuts at: 1678 5001 1678
Size: 3323 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2290 2322 2527 2559 2707
Size: 32 135 32 718 32 205 32 148
Cuts at: 2707 2720 2739 2752 3106 3113 3138 3145 4495
Size: 13 19 13 354 7 25 7 1350
Cuts at: 4495 4527 4569 4601 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 718 354 205 148 135 42
32 32 32 32 32 32 25 19
13 13 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3198 3444 669
Size: 988 281 1260 246 3079
Fragments arranged by size:
3079 1260 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5854
HpaI GTT'AAC
Cuts at: 3444 3444
Size: 5854
KpnI G_GTAC'C
Cuts at: 3179 3179
Size: 5854
MfeI C'AATT_G
Cuts at: 3453 3453
Size: 5854
MluI A'CGCG_T
Cuts at: 5730 5730
Size: 5854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4968 5152 1305
Size: 3663 184 2007
Fragments arranged by size:
3663 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2489 2810 10
Size: 54 1425 343 657 321 3054
Fragments arranged by size:
3054 1425 657 343 321 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2669 3084 3812 4171 4330 518
Size: 648 991 512 415 728 359 159 2042
Fragments arranged by size:
2042 991 728 648 512 415 359 159
MspA1I CmG'CkG
Cuts at: 1606 2865 3014 3270 3947 5095 5340 1606
Size: 1259 149 256 677 1148 245 2120
Fragments arranged by size:
2120 1259 1148 677 256 245 149
NcoI C'CATG_G
Cuts at: 513 1065 2004 2625 513
Size: 552 939 621 3742
Fragments arranged by size:
3742 939 621 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5854
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5854
NotI GC'GGCC_GC
Cuts at: 3301 3301
Size: 5854
NsiI A_TGCA'T
Cuts at: 2556 2556
Size: 5854
NspI r_CATG'y
Cuts at: 1132 1229 2558 2679 3212 4757 1132
Size: 97 1329 121 533 1545 2229
Fragments arranged by size:
2229 1545 1329 533 121 97
PasI CC'CwG_GG
Cuts at: 1348 2209 1348
Size: 861 4993
PciI A'CATG_T
Cuts at: 4753 4753
Size: 5854
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 1108
Size: 1329 4525
PfoI T'CCnGG_A
Cuts at: 2059 2343 3048 2059
Size: 284 705 4865
Fragments arranged by size:
4865 705 284
PmeI GTTT'AAAC
Cuts at: 3158 3158
Size: 5854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 3923 3955 5436 5468 2825
Size: 32 1066 32 1481 32 3211
Fragments arranged by size:
3211 1481 1066 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2287 3046 2287
Size: 759 5095
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5854
PsiI TTA'TAA
Cuts at: 2094 3424 2094
Size: 1330 4524
PspOMI G'GGCC_C
Cuts at: 2014 2014
Size: 5854
PstI C_TGCA'G
Cuts at: 830 2571 3019 3045 3206 830
Size: 1741 448 26 161 3478
Fragments arranged by size:
3478 1741 448 161 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5854
PvuII CAG'CTG
Cuts at: 3014 3014
Size: 5854
SacI G_AGCT'C
Cuts at: 2393 2421 2479 3033 2393
Size: 28 58 554 5214
Fragments arranged by size:
5214 554 58 28
SalI G'TCGA_C
Cuts at: 1936 3196 1936
Size: 1260 4594
SapI GCTCTTCn'nnn_
Cuts at: 2426 2426
Size: 5854
SbfI CC_TGCA'GG
Cuts at: 2571 3045 3206 2571
Size: 474 161 5219
Fragments arranged by size:
5219 474 161
ScaI AGT'ACT
Cuts at: 4102 5814 4102
Size: 1712 4142
SfcI C'TryA_G
Cuts at: 826 1045 2567 3015 3041 3202 4337 5018 5209
Size: 219 1522 448 26 161 1135 681 191
Cuts at: 5209 826
Size: 1471
Fragments arranged by size:
1522 1471 1135 681 448 219 191 161
26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5854
SmaI CCC'GGG
Cuts at: 1872 3181 1872
Size: 1309 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3958 4859 5121 820
Size: 197 926 2015 901 262 1553
Fragments arranged by size:
2015 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3645 493
Size: 3152 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5854
SphI G_CATG'C
Cuts at: 1132 2558 3212 1132
Size: 1426 654 3774
Fragments arranged by size:
3774 1426 654
SspI AAT'ATT
Cuts at: 5 52 3778 5
Size: 47 3726 2081
Fragments arranged by size:
3726 2081 47
StuI AGG'CCT
Cuts at: 1517 2651 1517
Size: 1134 4720
StyI C'CwwG_G
Cuts at: 513 1065 2004 2484 2625 2824 3293 513
Size: 552 939 480 141 199 469 3074
Fragments arranged by size:
3074 939 552 480 469 199 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 2794 3075 3881 3898 4051 4236
Size: 1255 1188 26 281 806 17 153 185
Cuts at: 4236 4681 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1188 806 445 281 185 153
26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4100 5812 96
Size: 274 80 33 51 1443 2123 1712 138
Fragments arranged by size:
2123 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5814 5846 5814
Size: 32 5822
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5854
XbaI T'CTAG_A
Cuts at: 3190 3190
Size: 5854
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5854
XmaI C'CCGG_G
Cuts at: 1870 3179 1870
Size: 1309 4545
XmnI GAAnn'nnTTC
Cuts at: 3983 3983
Size: 5854
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3662 276
Size: 53 83 186 936 2128 2468
Fragments arranged by size:
2468 2128 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI