Restriction map for
pFN21AE2547
(Circular) (Six-base) MAPSORT of: pFN21AE2547.seq Check: 3242 from: 1 to: 6097
pFN21AE2547 6097 bp
With 182 enzymes: SgfI *
October 24, 2014 11:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3440 1937
Size: 1503 4594
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2327 4160 5544 5812 1632
Size: 503 192 1833 1384 268 1917
Fragments arranged by size:
1917 1833 1384 503 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 4996 5973 1203
Size: 3793 977 1327
Fragments arranged by size:
3793 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AlwNI CAG_nnn'CTG
Cuts at: 3840 5412 3840
Size: 1572 4525
ApaLI G'TGCA_C
Cuts at: 2667 4154 5310 2667
Size: 1487 1156 3454
Fragments arranged by size:
3454 1487 1156
ApoI r'AATT_y
Cuts at: 1493 2057 2301 3406 3632 3784 5893 1493
Size: 564 244 1105 226 152 2109 1697
Fragments arranged by size:
2109 1697 1105 564 244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2255 3422 1870
Size: 73 312 1167 4545
Fragments arranged by size:
4545 1167 312 73
BamHI G'GATC_C
Cuts at: 3290 3427 3794 3290
Size: 137 367 5593
Fragments arranged by size:
5593 367 137
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3418 4873 618
Size: 325 217 627 1631 1455 1842
Fragments arranged by size:
1842 1631 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2858 2917 3065 3416 1391
Size: 1467 59 148 351 4072
Fragments arranged by size:
4072 1467 351 148 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2369 2403 4288 4322 1903
Size: 34 432 34 1885 34 3678
Fragments arranged by size:
3678 1885 432 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4708 136
Size: 107 122 71 631 605 3036 1525
Fragments arranged by size:
3036 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2108 6092 2108
Size: 3984 2113
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 2671 4158 5314 1790
Size: 881 1487 1156 2573
Fragments arranged by size:
2573 1487 1156 881
BmgBI CAC'GTC
Cuts at: 1318 2666 1318
Size: 1348 4749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2747 3828 4786
Size: 416 53 83 399 24 1330 1081 958
Cuts at: 4786 442
Size: 1753
Fragments arranged by size:
1753 1330 1081 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3012 4757 1239
Size: 1773 1745 2579
Fragments arranged by size:
2579 1773 1745
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2952 2980 2988 4760 882
Size: 617 1453 28 8 1772 2219
Fragments arranged by size:
2219 1772 1453 617 28 8
BsaAI yAC'GTr
Cuts at: 493 1204 3888 493
Size: 711 2684 2702
Fragments arranged by size:
2702 2684 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2749 2815 3904 4286
Size: 53 83 186 936 1216 66 1089 382
Cuts at: 4286 4921 275
Size: 635 1451
Fragments arranged by size:
1451 1216 1089 936 635 382 186 83
66 53
BsaWI w'CCGG_w
Cuts at: 3104 4530 4928 5202 5349 5760 3104
Size: 1426 398 274 147 411 3441
Fragments arranged by size:
3441 1426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5872
Fragments arranged by size:
5872 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2871 1186
Size: 1685 4412
BseYI C'CCAG_C
Cuts at: 2165 5300 5796 2165
Size: 3135 496 2466
Fragments arranged by size:
3135 2466 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3547 4308 5336 1540
Size: 275 186 898 648 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 648 275 186
BsiHKAI G_wGCw'C
Cuts at: 2671 2917 4158 4243 5314 2671
Size: 246 1241 85 1071 3454
Fragments arranged by size:
3454 1241 1071 246 85
BsmI GAATG_Cn'
Cuts at: 2142 2167 2402 2558 2799 3346 3608 3701 2142
Size: 25 235 156 241 547 262 93 4538
Fragments arranged by size:
4538 547 262 241 235 156 93 25
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2671 2858 2917 3065 3416 4158 4243
Size: 399 881 187 59 148 351 742 85
Cuts at: 4243 5314 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 881 742 399 351 187 148
85 59
BspEI T'CCGG_A
Cuts at: 3104 5760 3104
Size: 2656 3441
BspHI T'CATG_A
Cuts at: 3247 3986 5716 3247
Size: 739 1730 3628
Fragments arranged by size:
3628 1730 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3452 844
Size: 2608 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2377 3984 1394
Size: 429 554 1607 3507
Fragments arranged by size:
3507 1607 554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4741 4928 1700
Size: 110 2931 187 2869
Fragments arranged by size:
2931 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstXI CCAn_nnnn'nTGG
Cuts at: 3072 3196 3072
Size: 124 5973
BstYI r'GATC_y
Cuts at: 1972 2108 3203 3290 3427 3794 4179 4196 5648
Size: 136 1095 87 137 367 385 17 1452
Cuts at: 5648 5734 5746 6092 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1095 385 367 346 137 136
87 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 2178 2254 2355 3613 4407 4427 2178
Size: 76 101 1258 794 20 3848
Fragments arranged by size:
3848 1258 794 101 76 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 2104 3401 2104
Size: 1297 4800
DraIII CAC_nnn'GTG
Cuts at: 3098 3098
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2896 3544 4433
Size: 54 1425 50 275 18 1066 648 889
Cuts at: 4433 6062 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1066 889 648 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 3544 1537
Size: 275 1084 648 4090
Fragments arranged by size:
4090 1084 648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2325 2770 2830 2934 2974 4027 1353
Size: 729 243 445 60 104 40 1053 3423
Fragments arranged by size:
3423 1053 729 445 243 104 60 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2915 2915
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2327 3012 4160 4757 5544 5812
Size: 393 503 192 685 1148 597 787 268
Cuts at: 5812 1239
Size: 1524
Fragments arranged by size:
1524 1148 787 685 597 503 393 268
192
EcoNI CCTnn'n_nnAGG
Cuts at: 2862 3285 2862
Size: 423 5674
EcoO109I rG'GnC_Cy
Cuts at: 2335 2335
Size: 6097
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 5244 1678
Size: 3566 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 2845 2877 3249
Size: 32 135 32 1055 32 186 32 372
Cuts at: 3249 3281 4738 4770 4812 4844 1373
Size: 32 1457 32 42 32 2626
Fragments arranged by size:
2626 1457 1055 372 186 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3687 669
Size: 988 281 1503 246 3079
Fragments arranged by size:
3079 1503 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2540 748
Size: 1792 4305
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 2292 3696 2292
Size: 1404 4693
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5211 5395 1305
Size: 3906 184 2007
Fragments arranged by size:
3906 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4275
Fragments arranged by size:
4275 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3070 4055 4414 4573 518
Size: 648 1904 985 359 159 2042
Fragments arranged by size:
2042 1904 985 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3229 3513 4190 5338 5583 1606
Size: 1623 284 677 1148 245 2120
Fragments arranged by size:
2120 1623 1148 677 284 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
NdeI CA'TA_TG
Cuts at: 387 3142 387
Size: 2755 3342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 3455 5000 1132
Size: 97 2226 1545 2229
Fragments arranged by size:
2229 2226 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3098 1108
Size: 1990 4107
PfoI T'CCnGG_A
Cuts at: 2547 2884 2547
Size: 337 5760
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PstI C_TGCA'G
Cuts at: 830 2077 2343 3360 3449 830
Size: 1247 266 1017 89 3478
Fragments arranged by size:
3478 1247 1017 266 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
SacI G_AGCT'C
Cuts at: 2917 2917
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 2073 2339 3356 3445 4580 5261 5452
Size: 219 1028 266 1017 89 1135 681 191
Cuts at: 5452 826
Size: 1471
Fragments arranged by size:
1471 1135 1028 1017 681 266 219 191
89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4201 5102 5364 820
Size: 197 926 2258 901 262 1553
Fragments arranged by size:
2258 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SspI AAT'ATT
Cuts at: 5 52 2197 4021 5
Size: 47 2145 1824 2081
Fragments arranged by size:
2145 2081 1824 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 3536 513
Size: 552 939 321 1211 3074
Fragments arranged by size:
3074 1211 939 552 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2368 4124 4141 4294 4479 4924 325
Size: 1255 788 1756 17 153 185 445 1498
Fragments arranged by size:
1756 1498 1255 788 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4343 6055 96
Size: 274 80 33 51 1443 2366 1712 138
Fragments arranged by size:
2366 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2739 2771 6057 6089 2739
Size: 32 3286 32 2747
Fragments arranged by size:
3286 2747 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 2111 2831 3433 2111
Size: 720 602 4775
Fragments arranged by size:
4775 720 602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI