Restriction map for
pFN21AE2546
(Circular) (Six-base) MAPSORT of: pFN21AE2546.seq Check: 7043 from: 1 to: 8335
pFN21AE2546 8335 bp
With 182 enzymes: SgfI *
October 24, 2014 11:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6145 278
Size: 53 83 186 936 4609 2468
Fragments arranged by size:
4609 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 4324 5160 5678 1937
Size: 1464 923 836 518 4594
Fragments arranged by size:
4594 1464 923 836 518
Acc65I G'GTAC_C
Cuts at: 5656 5656
Size: 8335
AclI AA'CG_TT
Cuts at: 1563 6462 6835 1563
Size: 4899 373 3063
Fragments arranged by size:
4899 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 2577 2936 3237 3299 4332 4395 5366
Size: 551 394 359 301 62 1033 63 971
Cuts at: 5366 6398 7782 8050 1632
Size: 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1033 1032 971 551 394 359
301 268 63 62
AflII C'TTAA_G
Cuts at: 820 1017 4003 820
Size: 197 2986 5152
Fragments arranged by size:
5152 2986 197
AflIII A'CryG_T
Cuts at: 1203 3096 5038 7234 8211 1203
Size: 1893 1942 2196 977 1327
Fragments arranged by size:
2196 1942 1893 1327 977
AgeI A'CCGG_T
Cuts at: 7166 7166
Size: 8335
AhdI GACnn_n'nnGTC
Cuts at: 3397 7064 3397
Size: 3667 4668
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3134 5264 5343 1184
Size: 1950 2130 79 4176
Fragments arranged by size:
4176 2130 1950 79
AlwNI CAG_nnn'CTG
Cuts at: 5336 6078 7650 5336
Size: 742 1572 6021
Fragments arranged by size:
6021 1572 742
ApaI G_GGCC'C
Cuts at: 2087 2087
Size: 8335
ApaLI G'TGCA_C
Cuts at: 6392 7548 6392
Size: 1156 7179
ApoI r'AATT_y
Cuts at: 1493 2170 2285 2454 2983 3250 4388 4854 5135
Size: 677 115 169 529 267 1138 466 281
Cuts at: 5135 5204 5644 5870 6022 8131 1493
Size: 69 440 226 152 2109 1697
Fragments arranged by size:
2109 1697 1138 677 529 466 440 281
267 226 169 152 115 69
AseI AT'TA_AT
Cuts at: 160 1032 2232 3030 6889 160
Size: 872 1200 798 3859 1606
Fragments arranged by size:
3859 1606 1200 872 798
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8335
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 5660 1870
Size: 73 1248 2469 4545
Fragments arranged by size:
4545 2469 1248 73
BamHI G'GATC_C
Cuts at: 5665 6032 5665
Size: 367 7968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4867 5656 7111 618
Size: 325 217 627 3080 789 1455 1842
Fragments arranged by size:
3080 1842 1455 789 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 5654 1391
Size: 696 3567 4072
Fragments arranged by size:
4072 3567 696
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3042 3480 4566 928
Size: 971 1143 438 1086 4697
Fragments arranged by size:
4697 1143 1086 971 438
BbvCI CC'TCA_GC
Cuts at: 2689 4935 5439 2689
Size: 2246 504 5585
Fragments arranged by size:
5585 2246 504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6526 6560 1903
Size: 34 4589 34 3678
Fragments arranged by size:
4589 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6227 7437 6227
Size: 1210 7125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8335
BfrBI ATG'CAT
Cuts at: 2918 2918
Size: 8335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6946 136
Size: 107 122 71 631 605 5274 1525
Fragments arranged by size:
5274 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2158 3302 3448 8330 2158
Size: 1144 146 4882 2163
Fragments arranged by size:
4882 2163 1144 146
BlpI GC'TnA_GC
Cuts at: 2367 5067 5752 2367
Size: 2700 685 4950
Fragments arranged by size:
4950 2700 685
Bme1580I G_kGCm'C
Cuts at: 1790 2087 4777 6396 7552 1790
Size: 297 2690 1619 1156 2573
Fragments arranged by size:
2690 2573 1619 1156 297
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2760 6066 7024
Size: 416 53 83 399 24 1343 3306 958
Cuts at: 7024 442
Size: 1753
Fragments arranged by size:
3306 1753 1343 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3586 5202 6995 1239
Size: 2347 1616 1793 2579
Fragments arranged by size:
2579 2347 1793 1616
Bpu10I CC'TnA_GC
Cuts at: 2689 4935 5439 2689
Size: 2246 504 5585
Fragments arranged by size:
5585 2246 504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2868 6460 7325 7623 2249
Size: 619 3592 865 298 2961
Fragments arranged by size:
3592 2961 865 619 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3749 4703 6998 882
Size: 617 2250 954 2295 2219
Fragments arranged by size:
2295 2250 2219 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 6126 493
Size: 711 4922 2702
Fragments arranged by size:
4922 2702 711
BsaBI GATnn'nnATC
Cuts at: 5516 8329 5516
Size: 2813 5522
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6142 6524 7159 275
Size: 53 83 186 936 4609 382 635 1451
Fragments arranged by size:
4609 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6768 7166 7440 7587 7998 6768
Size: 398 274 147 411 7105
Fragments arranged by size:
7105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5200 5230 1178
Size: 30 165 30 3797 30 4283
Fragments arranged by size:
4283 3797 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2834 3567 5493 5496 1186
Size: 1648 733 1926 3 4025
Fragments arranged by size:
4025 1926 1648 733 3
BseYI C'CCAG_C
Cuts at: 3893 5413 7538 8034 3893
Size: 1520 2125 496 4194
Fragments arranged by size:
4194 2125 1520 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 2878 1163
Size: 573 603 539 6620
Fragments arranged by size:
6620 603 573 539
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5785 6546 7574 1540
Size: 275 186 3784 761 1028 2301
Fragments arranged by size:
3784 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2395 6396 6481 7552 2395
Size: 4001 85 1071 3178
Fragments arranged by size:
4001 3178 1071 85
BsmI GAATG_Cn'
Cuts at: 5846 5939 5846
Size: 93 8242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2395 4777 5654 6396 6481 7552
Size: 399 297 308 2382 877 742 85 1071
Cuts at: 7552 1391
Size: 2174
Fragments arranged by size:
2382 2174 1071 877 742 399 308 297
85
BspEI T'CCGG_A
Cuts at: 7998 7998
Size: 8335
BspHI T'CATG_A
Cuts at: 4290 6224 7954 4290
Size: 1934 1730 4671
Fragments arranged by size:
4671 1934 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5690 844
Size: 4846 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6222 1394
Size: 429 4399 3507
Fragments arranged by size:
4399 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2859 6830 7004 65
Size: 1183 1611 3971 174 1396
Fragments arranged by size:
3971 1611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6979 7166 1700
Size: 110 5169 187 2869
Fragments arranged by size:
5169 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8335
BssSI C'ACGA_G
Cuts at: 6395 7407 6395
Size: 1012 7323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5173 5257 5336 794
Size: 4379 84 79 3793
Fragments arranged by size:
4379 3793 84 79
BstBI TT'CG_AA
Cuts at: 7139 7139
Size: 8335
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8335
BstXI CCAn_nnnn'nTGG
Cuts at: 4977 4977
Size: 8335
BstYI r'GATC_y
Cuts at: 1972 2158 2560 3302 3448 5665 6032 6417 6434
Size: 186 402 742 146 2217 367 385 17
Cuts at: 6434 7886 7972 7984 8330 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2217 1977 1452 742 402 385 367 346
186 146 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6844
Fragments arranged by size:
6844 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7596
Fragments arranged by size:
7596 683 56
BtsI GCAGTG_nn'
Cuts at: 5588 5851 6645 6665 5588
Size: 263 794 20 7258
Fragments arranged by size:
7258 794 263 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3073 3108 529
Size: 35 2509 35 5756
Fragments arranged by size:
5756 2509 35 35
DraI TTT'AAA
Cuts at: 4107 4362 5374 5639 4107
Size: 255 1012 265 6803
Fragments arranged by size:
6803 1012 265 255
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7342 809
Size: 507 6026 1802
Fragments arranged by size:
6026 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3320 5190 5782
Size: 54 1425 50 275 18 1490 1870 592
Cuts at: 5782 6671 8300 8
Size: 889 1629 43
Fragments arranged by size:
1870 1629 1490 1425 889 592 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5782 1537
Size: 275 3970 4090
Fragments arranged by size:
4090 3970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 3213 5636 6265 1353
Size: 1167 693 2423 629 3423
Fragments arranged by size:
3423 2423 1167 693 629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6922 7296 7442 387
Size: 1064 138 5333 374 146 1280
Fragments arranged by size:
5333 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2577 2936 3237 3299 3586 4332
Size: 393 551 394 359 301 62 287 746
Cuts at: 4332 4395 5202 5366 6398 6995 7782 8050 1239
Size: 63 807 164 1032 597 787 268 1524
Fragments arranged by size:
1524 1032 807 787 746 597 551 394
393 359 301 287 268 164 63 62
EcoO109I rG'GnC_Cy
Cuts at: 2083 2084 4939 2083
Size: 1 2855 5479
Fragments arranged by size:
5479 2855 1
EcoRI G'AATT_C
Cuts at: 5644 6022 5644
Size: 378 7957
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3457 3457
Size: 8335
FspI TGC'GCA
Cuts at: 6841 6841
Size: 8335
HaeII r_GCGC'y
Cuts at: 1678 7482 1678
Size: 5804 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2622 2654 2708 2740 3098
Size: 32 135 32 1050 32 54 32 358
Cuts at: 3098 3130 3383 3415 3584 3616 4243 4275 5458
Size: 32 253 32 169 32 627 32 1183
Cuts at: 5458 5490 6976 7008 7050 7082 1373
Size: 32 1486 32 42 32 2626
Fragments arranged by size:
2626 1486 1183 1050 627 358 253 169
135 54 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3226 5679 5925 669
Size: 988 281 1288 2453 246 3079
Fragments arranged by size:
3079 2453 1288 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4219 748
Size: 3471 4864
HpaI GTT'AAC
Cuts at: 3226 5925 3226
Size: 2699 5636
KpnI G_GTAC'C
Cuts at: 5660 5660
Size: 8335
MfeI C'AATT_G
Cuts at: 4791 5934 4791
Size: 1143 7192
MluI A'CGCG_T
Cuts at: 8211 8211
Size: 8335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2371 4160 7449 7633 1305
Size: 1066 1789 3289 184 2007
Fragments arranged by size:
3289 2007 1789 1066 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3322 5192 10
Size: 54 1425 343 1490 1870 3153
Fragments arranged by size:
3153 1870 1490 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3116 3482 5043 6293 6652 6811 518
Size: 648 1950 366 1561 1250 359 159 2042
Fragments arranged by size:
2042 1950 1561 1250 648 366 359 159
MspA1I CmG'CkG
Cuts at: 1606 2693 5066 5222 5333 5342 5348 5360 5751
Size: 1087 2373 156 111 9 6 12 391
Cuts at: 5751 6428 7576 7821 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2373 2120 1148 1087 677 391 245 156
111 12 9 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6844
Fragments arranged by size:
6844 939 552
NdeI CA'TA_TG
Cuts at: 387 2239 3825 387
Size: 1852 1586 4897
Fragments arranged by size:
4897 1852 1586
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8335
NotI GC'GGCC_GC
Cuts at: 5782 5782
Size: 8335
NsiI A_TGCA'T
Cuts at: 2920 2920
Size: 8335
NspI r_CATG'y
Cuts at: 1132 1229 3100 5042 5188 5693 7238 1132
Size: 97 1871 1942 146 505 1545 2229
Fragments arranged by size:
2229 1942 1871 1545 505 146 97
PasI CC'CwG_GG
Cuts at: 1348 2617 1348
Size: 1269 7066
PciI A'CATG_T
Cuts at: 3096 5038 7234 3096
Size: 1942 2196 4197
Fragments arranged by size:
4197 2196 1942
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8335
PfoI T'CCnGG_A
Cuts at: 2312 2395 2312
Size: 83 8252
PmeI GTTT'AAAC
Cuts at: 5639 5639
Size: 8335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6404 6436 7917 7949 6404
Size: 32 1481 32 6790
Fragments arranged by size:
6790 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8335
PsiI TTA'TAA
Cuts at: 4989 5905 4989
Size: 916 7419
PspOMI G'GGCC_C
Cuts at: 2083 2083
Size: 8335
PstI C_TGCA'G
Cuts at: 830 2698 3943 5178 5253 5332 5341 5347 5353
Size: 1868 1245 1235 75 79 9 6 6
Cuts at: 5353 5365 5687 830
Size: 12 322 3478
Fragments arranged by size:
3478 1868 1245 1235 322 79 75 12
9 6 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8335
PvuII CAG'CTG
Cuts at: 2693 5066 5222 5333 5342 5348 5360 2693
Size: 2373 156 111 9 6 12 5668
Fragments arranged by size:
5668 2373 156 111 12 9 6
SalI G'TCGA_C
Cuts at: 1936 5677 1936
Size: 3741 4594
SbfI CC_TGCA'GG
Cuts at: 5687 5687
Size: 8335
ScaI AGT'ACT
Cuts at: 2201 4408 6583 8295 2201
Size: 2207 2175 1712 2241
Fragments arranged by size:
2241 2207 2175 1712
SfcI C'TryA_G
Cuts at: 826 1045 2694 3306 3367 3516 3939 4201 5174
Size: 219 1649 612 61 149 423 262 973
Cuts at: 5174 5249 5328 5337 5343 5349 5361 5683 6818
Size: 75 79 9 6 6 12 322 1135
Cuts at: 6818 7499 7690 826
Size: 681 191 1471
Fragments arranged by size:
1649 1471 1135 973 681 612 423 322
262 219 191 149 79 75 61 12
9 6 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8335
SmaI CCC'GGG
Cuts at: 1872 5662 1872
Size: 3790 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 2883 4003 6439 7340 7602
Size: 197 926 321 619 1120 2436 901 262
Cuts at: 7602 820
Size: 1553
Fragments arranged by size:
2436 1553 1120 926 901 619 321 262
197
SnaBI TAC'GTA
Cuts at: 493 6126 493
Size: 5633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8335
SphI G_CATG'C
Cuts at: 1132 5693 1132
Size: 4561 3774
SspI AAT'ATT
Cuts at: 5 52 4876 6259 5
Size: 47 4824 1383 2081
Fragments arranged by size:
4824 2081 1383 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8335
StyI C'CwwG_G
Cuts at: 513 1065 2004 5774 513
Size: 552 939 3770 3074
Fragments arranged by size:
3770 3074 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5479 6362 6379 6532 6717 7162 325
Size: 1255 3899 883 17 153 185 445 1498
Fragments arranged by size:
3899 1498 1255 883 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 4406 6581
Size: 274 80 33 51 1443 222 2207 2175
Cuts at: 6581 8293 96
Size: 1712 138
Fragments arranged by size:
2207 2175 1712 1443 274 222 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8295 8327 8295
Size: 32 8303
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8335
XbaI T'CTAG_A
Cuts at: 3421 3721 4163 5671 3421
Size: 300 442 1508 6085
Fragments arranged by size:
6085 1508 442 300
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8335
XmaI C'CCGG_G
Cuts at: 1870 5660 1870
Size: 3790 4545
XmnI GAAnn'nnTTC
Cuts at: 2874 6464 2874
Size: 3590 4745
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6143 276
Size: 53 83 186 936 4609 2468
Fragments arranged by size:
4609 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI